Specificity of the bacteriophage PBS2 induced inhibitor of uracil-DNA glycosylase.

abstract：:For the elucidation of the mechanism of steroid hormone receptor activation, the hydrodynamic properties of the unactivated and activated forms of the nonproteolyzed glucocorticoid receptor from the mouse AtT-20 pituitary tumor cell line were determined. The unactivated, molybdate-stabilized receptor has the following...

journal_title：Biochemistry

authors： Vedeckis WV

更新日期：1983-04-12 00:00:00

abstract：:Six structural repeat motifs of 58 amino acids are found in the sequence of both mouse and human properdins. Twelve more examples of the motif are available from the sequences of thrombospondin, the terminal complement components, and the thrombospondin-related anonymous protein. The averaged Robson and Chou-Fasman se...

journal_title：Biochemistry

authors： Perkins SJ,Nealis AS,Haris PI,Chapman D,Goundis D,Reid KB

更新日期：1989-09-05 00:00:00

abstract：:The crystal structures of metyrapone- and 1-, 2-, and 4-phenylimidazole-inhibited complexes of cytochrome P-450cam have been refined to a nominal resolution of 2.1 A and compared with the 1.63-A camphor-bound structure. With the exception of 2-phenylimidazole, each of the inhibitors forms an N-Fe bond with the heme ir...

journal_title：Biochemistry

authors： Poulos TL,Howard AJ

更新日期：1987-12-15 00:00:00

abstract：:The intact photosystem I of maize containing its full antenna complement (PSI-200) has been purified and fractionated into the core and outer antenna (LHCI) components. It is demonstrated by absorption and fluorescence spectroscopy that at least 80% of the long wavelength absorbing antenna pigments (red forms) are loc...

journal_title：Biochemistry

authors： Croce R,Zucchelli G,Garlaschi FM,Jennings RC

更新日期：1998-12-15 00:00:00

abstract：:Molecular interactions between the tumor suppressor p53 and the transcriptional coactivators CBP/p300 are critical for the regulation of p53 transactivation and stability. The transactivation domain (TAD) of p53 binds directly to several CBP/p300 domains (TAZ1, TAZ2, NCBD, and KIX). Here we map the interaction between...

journal_title：Biochemistry

authors： Lee CW,Arai M,Martinez-Yamout MA,Dyson HJ,Wright PE

更新日期：2009-03-17 00:00:00

abstract：:In order to compare the interfacial behavior of the polyheme cytochromes c which belong to the cytochrome c3 superfamily, the monomolecular film technique was used to determine whether and how these metalloproteins interact with (phospho)lipids). Measurements of the variations of surface pressure and surface potential...

journal_title：Biochemistry

authors： Florens L,Ivanova M,Dolla A,Czjzek M,Haser R,Verger R,Bruschi M

更新日期：1995-09-12 00:00:00

abstract：:The generation of multiprotein complexes at receptors and adapter proteins is crucial for the activation of intracellular signaling pathways. In this study, we used multiple biochemical and biophysical methods to examine the binding properties of several SH2 and SH3 domain-containing signaling proteins as they interac...

journal_title：Biochemistry

authors： Houtman JC,Higashimoto Y,Dimasi N,Cho S,Yamaguchi H,Bowden B,Regan C,Malchiodi EL,Mariuzza R,Schuck P,Appella E,Samelson LE

更新日期：2004-04-13 00:00:00

abstract：:The DNA binding domain (DBD) of p53 folds by a complex mechanism that involves parallel pathways and multiple intermediates, both on- and off-pathway. This heterogeneity renders DBD particularly susceptible to misfolding and aggregation. The origins of parallel folding mechanisms are not well understood. DBD folding h...

journal_title：Biochemistry

authors： Butler JS,Loh SN

更新日期：2007-03-13 00:00:00

abstract：:The bidirectional transformation of a protein between its native water-soluble and integral transmembrane conformations is demonstrated for FraC, a hemolytic protein of the family of pore-forming toxins. In the presence of biological membranes, the water-soluble conformation of FraC undergoes a remarkable structural r...

journal_title：Biochemistry

authors： Tanaka K,Caaveiro JM,Tsumoto K

更新日期：2015-11-24 00:00:00

abstract：:The fungal neurotoxin alpha-cyclopiazonic acid (CPA), a nanomolar inhibitor of Ca(2+)-ATPase with a unique pentacyclic indole tetramic acid scaffold, is assembled by a three enzyme pathway CpaS, CpaD, and CpaO in Aspergillus sp. We recently characterized the first pathway-specific enzyme CpaS, a hybrid two module poly...

journal_title：Biochemistry

authors： Liu X,Walsh CT

更新日期：2009-11-24 00:00:00

abstract：:The selective paramagnetic relaxation of oligonucleotide protons of d(GTGCAC)2 by delta- and lambda-Ni(phen)3(3+) and delta- and lambda-Cr(phen)3(3+) has been examined to obtain some structural insight into the noncovalent binding of tris(phenanthroline) metal complexes to DNA. The experiments demonstrate that the rel...

journal_title：Biochemistry

authors： Rehmann JP,Barton JK

更新日期：1990-02-20 00:00:00

abstract：:Administration of pregnenolone-16 alpha-carbonitrile (PCN) to adult female rats caused a 2-fold increase in total liver microsomal cytochrome P-450 along with 5-7-fold increases in four in vitro monooxygenase activities considered diagnostic for the major PCN-inducible cytochrome P-450 isozyme. However, upon administr...

journal_title：Biochemistry

authors： Graves PE,Kaminsky LS,Halpert J

更新日期：1987-06-30 00:00:00

abstract：:Deuterium magnetic resonance spectra (55.26 MHz) of cholesterol-3 alpha-d1 and epicholesterol-3 beta-d1 in dipalmitoylglycerophosphocholine (DPPC) liposomes were measured as a function of sterol-to-phospholipid ratio below (24 degrees C) and above (60 degrees C) the phase transition temperature of DPPC. From the quadr...

journal_title：Biochemistry

authors： Murari R,Murari MP,Baumann WJ

更新日期：1986-03-11 00:00:00

abstract：:The rotational motions of immunoglobulin M (IgM) were investigated by the nanosecond fluorescence depolarization technique. The fluorophore epsilon-1-dimethylamino-5-naphthalenesulfonyl-L-lysine (DNS-lysine) was specifically bound in the combining sites of anti-DNS IgM antibodies from the horse, pig, and nurse shark. ...

journal_title：Biochemistry

authors： Holowka DA,Cathou RE

更新日期：1976-07-27 00:00:00

abstract：:It is known that the overall charge of a protein can change as the molecule approaches a charged object like another protein or a cell membrane. We have formalized this mechanism using a statistical mechanical framework and show how this rather overlooked interaction increases the attraction between protein molecules....

journal_title：Biochemistry

authors： Lund M,Jönsson B

更新日期：2005-04-19 00:00:00

abstract：:Protein disulfide-isomerase, a protein localized to the lumen of the endoplasmic reticulum of eukaryotic cells, catalyzes the posttranslational formation and rearrangement of protein disulfide bonds. As isolated from bovine liver, the enzyme contains 0.8 free sulfhydryl group per mole of protein monomer and 3.1 disulf...

journal_title：Biochemistry

authors： Gilbert HF

更新日期：1989-09-05 00:00:00

abstract：:The bacterial second messenger signaling molecule bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) controls important biological processes such as biofilm formation, virulence response, and motility. This second messenger is sensed by macromolecular targets inside the cell, both protein and RNA, which ind...

journal_title：Biochemistry

authors： Shanahan CA,Gaffney BL,Jones RA,Strobel SA

更新日期：2013-01-15 00:00:00

abstract：:Nonribosomal peptide synthetases (NRPSs) are megasynthetases that require complex and specific interactions between multiple domains and proteins to functionally produce a metabolite. MbtH-like proteins (MLPs) are integral components of many NRPSs and interact directly with the adenylation domain of the megasynthetase...

journal_title：Biochemistry

authors： Schomer RA,Park H,Barkei JJ,Thomas MG

更新日期：2018-07-17 00:00:00

abstract：:Formylglycinamide ribonucleotide amidotransferase (FGAR-AT) catalyzes the ATP- and glutamine-dependent formation of formylglycinamidine ribonucleotide, ADP, P(i), and glutamate in the fourth step of de novo purine biosynthesis. Like all amidotransferases (ATs), FGAR-AT is proposed to channel ammonia between a glutamin...

journal_title：Biochemistry

authors： Hoskins AA,Anand R,Ealick SE,Stubbe J

更新日期：2004-08-17 00:00:00

abstract：:Equilibrium binding isotherms have been measured as a function of temperature for the interaction of 2- and 8-aminoadenosine with poly(U) by continuous, constant-volume ultrafiltration. An analytical method is described for determining the entire isotherm in a single experiment on a preequilibrated ligand--polymer sol...

journal_title：Biochemistry

authors： Roy KB,Miles HT

更新日期：1982-01-05 00:00:00

abstract：:Lateral mobilities of fluorescent cell surface probes have been measured on normal (3T3) and transformed (SV3T3) cultured mouse fibroblasts. There is little discernible difference in the mobilities of a lipid analogue (diI), a fluorescent ganglioside derivative (GM1), and tetramethylrhodamine-labeled succinylated conc...

journal_title：Biochemistry

authors： Eldridge CA,Elson EL,Webb WW

更新日期：1980-05-13 00:00:00

abstract：:High-resolution proton nuclear magnetic resonance (1H NMR) measurements were made on myosin, heavy meromyosin (HMM), myosin subfragment 1 (S1), light meromyosin (LMM), and actin. A strong signal from amino acid side chains undergoing motions too fast to be accounted for by simple rotations of groups on a rigid backbon...

journal_title：Biochemistry

authors： Highsmith S,Akasaka K,Konrad M,Goody R,Holmes K,Wade-Jardetzky N,Jardetzky O

更新日期：1979-09-18 00:00:00

abstract：:The human pathogen Staphylococcus aureus acquires heme iron from hemoglobin (Hb) via the action of a series of iron-regulated surface determinant (Isd) proteins. The cell wall anchored IsdB protein is recognized as the predominant Hb receptor, and is comprised of two NEAr transporter (NEAT) domains that act in concert...

journal_title：Biochemistry

authors： Fonner BA,Tripet BP,Eilers BJ,Stanisich J,Sullivan-Springhetti RK,Moore R,Liu M,Lei B,Copié V

更新日期：2014-06-24 00:00:00

abstract：:The binding of the R17 coat protein to synthetic RNAs containing one or two coat protein binding sites was characterized by using nitrocellulose filter and gel-retention assays. RNAs with two available sites bound coat protein in a cooperative manner, resulting in a higher affinity and reduced sensitivity to pH, ionic...

journal_title：Biochemistry

authors： Witherell GW,Wu HN,Uhlenbeck OC

更新日期：1990-12-18 00:00:00

abstract：:The ratio of the second-order rate constants for reduction of dihydroxyacetone phosphate (DHAP) and of the neutral truncated substrate glycolaldehyde (GLY) by glycerol 3-phosphate dehydrogenase (NAD (+), GPDH) saturated with NADH is (1.0 x 10 (6) M (-1) s (-1))/(8.7 x 10 (-3) M (-1) s (-1)) = 1.1 x 10 (8), which was u...

journal_title：Biochemistry

authors： Tsang WY,Amyes TL,Richard JP

更新日期：2008-04-22 00:00:00

abstract：:Glycophorin A is a sialoglycoprotein isolated from human erythrocyte membranes which seems to exist as stable dimeric complexes in the presence of sodium dodecyl sulfate. When analyzed by dodecyl sulfate acrylamide electrophoresis this molecule forms two PAS-stainable bands (PAS-U and PAS-2) which are reversibly inter...

journal_title：Biochemistry

authors： Furthmayr H,Marchesi VT

更新日期：1976-03-09 00:00:00

abstract：:Time-resolved resonance Raman spectroscopy has been employed to monitor geminate heme-CO rebinding in photolyzed HbCO. The excitation frequency was tuned to enhance the scattering from rebound heme sites 20-500 ns subsequent to CO photolysis. The behavior of vFe-C during ligand rebinding has important ramifications co...

journal_title：Biochemistry

authors： Schneebeck MC,Vigil LE,Friedman JM,Chavez MD,Ondrias MR

更新日期：1993-02-09 00:00:00

abstract：:Database searches indicated that the genome of Bacillus subtilis contains three different genes encoding RNase H homologues. The ypdQ gene encodes an RNase HI homologue with 132 amino acid residues, whereas the rnh and ysgB genes encode RNase HII homologues with 255 and 313 amino acid residues, respectively. RNases HI...

journal_title：Biochemistry

authors： Ohtani N,Haruki M,Morikawa M,Crouch RJ,Itaya M,Kanaya S

更新日期：1999-01-12 00:00:00

abstract：:As part of a search for peptides that have specificity for selected protein kinases, the possibility that adenosine cyclic 3',5'-phosphate dependent protein kinase (A-kinase) recognizes the hydrogen-bonding potential of its peptide substrates was investigated. A-Kinase catalyzes the phosphorylation of five N alpha-met...

journal_title：Biochemistry

authors： Thomas NE,Bramson HN,Miller WT,Kaiser ET

更新日期：1987-07-14 00:00:00

abstract：:The highly processive motor, myosin V, has an extremely long neck containing six calmodulin-binding IQ motifs that allows it to take multiple 36 nm steps corresponding to the pseudo-repeat of actin. To further investigate how myosin V moves processively on actin filaments, we altered the length of the neck by adding o...

journal_title：Biochemistry

authors： Sakamoto T,Yildez A,Selvin PR,Sellers JR

更新日期：2005-12-13 00:00:00