当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Kinetics and equilibria for the reactions of coenzymes with wild type and the Y70F mutant of Escherichia coli aspartate aminotransferase.

Kinetics and equilibria for the reactions of coenzymes with wild type and the Y70F mutant of Escherichia coli aspartate aminotransferase.

Abstract：

:The Y70F mutant of aspartate aminotransferase has reduced affinity for coenzymes compared to the wild type. The equilibrium dissociation constants for pyridoxamine phosphate (PMP) holoenzymes, KPMPdiss, were determined from the association and dissociation rate constants to be 1.3 nM and 30 nM for the wild type and mutant, respectively. This increase in KPMPdiss for Y70F is due to a 27-fold increase in the dissociation rate constant. Pyridoxal phosphate (PLP) association kinetics are complex, with three kinetic processes detectable for wild type and two for Y70F. A directly determined, accurate value of KPLPdiss for wild type enzyme has been difficult to obtain because of the low value of this constant. The values of KPLPdiss for the holoenzymes were determined indirectly through the measured values for KPMPdiss, glutamate-alpha-ketoglutarate half-reaction equilibrium constants, and the equilibrium constant for the transamination of PLP by glutamate catalyzed by Y70F. The values of KPLPdiss obtained by this procedure are 0.4 pM for wild type and 40 pM for Y70F. The increases in KPMPdiss and KPLPdiss for Y70F correspond to delta delta G values of 1.9 and 2.7 kcal/mol, respectively, and are directly attributed to the loss of the hydrogen bond from the phenolic hydroxyl group of Tyr70 to the coenzyme phosphate. The delta G for association of PLP with wild type enzyme is 4.7 kcal/mol more favorable than that for PMP.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Toney MD,Kirsch JF

doi

10.1021/bi00244a014

subject

Has Abstract

pub_date

1991-07-30 00:00:00

pages

7461-6

issue

30

eissn

0006-2960

issn

1520-4995

journal_volume

30

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Revealing Conformational Transitions in G-Protein-Coupled Receptor Rhodopsin upon Phosphorylation.

    abstract：:G-protein-coupled receptors (GPCRs) have evolved as highly specialized cellular machinery that can dictate biological outcomes in response to diverse stimuli. Specifically, they induce multiple pathway responses upon structural perturbations induced at local protein sites. GPCRs utilize a concurrent strategy involving...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00884

    authors： Jatana N,Aswin SK,Rathore S,Thukral L

    更新日期：2020-01-28 00:00:00

  • Conformation of a rigid tetrasaccharide epitope in the capsular polysaccharide of Vibrio cholerae O139.

    abstract：:A newly reported strain of Vibrio cholerae, known as strain O139 Bengal, is the first instance of an encapsulated strain that has caused epidemic cholera. The O-antigenic capsule is the critical antigen for protective immunity. Since mapping of the antigenic epitopes will assist in the development of a protein conjuga...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9910272

    authors： Gunawardena S,Fiore CR,Johnson JA,Bush CA

    更新日期：1999-09-14 00:00:00

  • Characterization and kinetic mechanism of catalytic domain of human vascular endothelial growth factor receptor-2 tyrosine kinase (VEGFR2 TK), a key enzyme in angiogenesis.

    abstract：:Vascular endothelial growth factor (VEGF) is a dimeric protein which induces formation of new blood vessels (angiogenesis) through binding to VEGF-receptor-2 tyrosine kinase (VEGFR2 TK) or KDR (kinase insert domain-containing receptor) on the surface of endothelial cells. Angiogenesis has been shown to be essential fo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981291f

    authors： Parast CV,Mroczkowski B,Pinko C,Misialek S,Khambatta G,Appelt K

    更新日期：1998-11-24 00:00:00

  • Identification of Factors Complicating Bioluminescence Imaging.

    abstract：:In vivo bioluminescence imaging (BLI) has become a standard, non-invasive imaging modality for following gene expression or the fate of proteins and cells in living animals. Currently, bioluminescent reporters used in laboratories are mostly derivatives of two major luciferase families: ATP-dependent insect luciferase...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b01303

    authors： Yeh HW,Wu T,Chen M,Ai HW

    更新日期：2019-03-26 00:00:00

  • Small Molecule Rescue and Glycosidic Conformational Analysis of the Twister Ribozyme.

    abstract：:The number of self-cleaving ribozymes has increased sharply in recent years, giving rise to elaborations of the four known ribozyme catalytic strategies, α, β, γ, and δ. One such extension is utilized by the twister ribozyme, which is hypothesized to conduct δ, or general acid catalysis, via N3 of the syn adenine +1 n...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00742

    authors： Messina KJ,Kierzek R,Tracey MA,Bevilacqua PC

    更新日期：2019-12-03 00:00:00

  • Neuronal Synapses: Microscale Signal Processing Machineries Formed by Phase Separation?

    abstract：:The organization principles underlying non-membrane-bound organelles have started to unravel in the past 10 years. A new biophysical model known as biomolecular condensates has been proposed to explain many aspects of membraneless organelle assembly and regulation. Neurons are extremely complex, and each neuron can co...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/acs.biochem.8b00313

    authors： Feng Z,Zeng M,Chen X,Zhang M

    更新日期：2018-05-01 00:00:00

  • Conformational changes in the amino-terminal helix of the G protein alpha(i1) following dissociation from Gbetagamma subunit and activation.

    abstract：:G protein alpha subunits mediate activation of signaling pathways through G protein-coupled receptors (GPCR) by virtue of GTP-dependent conformational rearrangements. It is known that regions of disorder in crystal structures can be indicative of conformational flexibility within a molecule, and there are several such...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0255726

    authors： Medkova M,Preininger AM,Yu NJ,Hubbell WL,Hamm HE

    更新日期：2002-08-06 00:00:00

  • The Streptomyces-produced antibiotic fosfomycin is a promiscuous substrate for archaeal isopentenyl phosphate kinase.

    abstract：:Isopentenyl phosphate kinase (IPK) catalyzes the phosphorylation of isopentenyl phosphate to form the isoprenoid precursor isopentenyl diphosphate in the archaeal mevalonate pathway. This enzyme is highly homologous to fosfomycin kinase (FomA), an antibiotic resistance enzyme found in a few strains of Streptomyces and...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201662k

    authors： Mabanglo MF,Serohijos AW,Poulter CD

    更新日期：2012-01-31 00:00:00

  • Identification of proximal and distal axial ligands in Leishmania major pseudoperoxidase.

    abstract：:Previous optical and electron paramagnetic resonance (EPR) spectroscopic studies of the newly discovered peroxynitrite scavenging pseudoperoxidase from Leishmania major (LmPP) suggested that ferric LmPP contained a six-coordinate low-spin (6cLS) heme with a thiolate ligand, presumably a cysteine, bound to its heme iro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi401343t

    authors： Saha R,Bose M,Sen Santara S,Roy J,Adak S

    更新日期：2013-12-10 00:00:00

  • The acidic phosphoproteins from Saccharomyces cerevisiae ribosomes. NH2-terminal acetylation is a conserved difference between P1 and P2 proteins.

    abstract：:Isoelectrofocusing gels of acidic ribosomal proteins from most yeast strains reveal the presence of up to 10 bands which are the product of only 4 genes. The proteins have been characterized by NH2-terminal amino acid sequencing, specific antibodies, HPLC, and by taking advantage of acidic protein-defective yeast stra...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00067a010

    authors： Santos C,Ortiz-Reyes B,Naranda T,Remacha M,Ballesta JP

    更新日期：1993-04-27 00:00:00

  • Catalytic reaction profile for alcohol oxidation by galactose oxidase.

    abstract：:Galactose oxidase is a remarkable enzyme containing a metalloradical redox cofactor capable of oxidizing a variety of primary alcohols during enzyme turnover. Recent studies using 1-O-methyl alpha-D-galactopyranoside have revealed an unusually large kinetic isotope effect (KIE) for oxidation of the alpha-deuterated al...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010303l

    authors： Whittaker MM,Whittaker JW

    更新日期：2001-06-19 00:00:00

  • Characterization of Caenorhabditis elegans Nucleosome Assembly Protein 1 Uncovers the Role of Acidic Tails in Histone Binding.

    abstract：:Nucleosome assembly proteins (Naps) influence chromatin dynamics by directly binding to histones. Here we provide a comprehensive structural and biochemical analysis of a Nap protein from Caenorhabditis elegans (CeNap1). CeNap1 naturally lacks the acidic N-terminal tail and has a short C-terminal tail compared to many...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b01033

    authors： Sarkar P,Zhang N,Bhattacharyya S,Salvador K,D'Arcy S

    更新日期：2019-01-15 00:00:00

  • Solution structure of decorin-binding protein A from Borrelia burgdorferi.

    abstract：:Decorin-binding protein A (DBPA) is an important lipoprotein from the bacterium Borrelia burgdorferi, the causative agent of Lyme disease. The absence of DBPA drastically reduces the pathogenic potential of the bacterium, and biochemical evidence indicates DBPA's interactions with the glycosaminoglycan (GAG) portion o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi3007093

    authors： Wang X

    更新日期：2012-10-23 00:00:00

  • The sucrose permease of Escherichia coli: functional significance of cysteine residues and properties of a cysteine-less transporter.

    abstract：:The sucrose (CscB) permease belongs to the oligosaccharide:H(+) symporter family of the Major Facilitator Superfamily and is homologous to the lactose permease from Escherichia coli. Sucrose transport in cells expressing sucrose permease is completely inhibited by N-ethylmaleimide (NEM), suggesting that one or more of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000124o

    authors： Sahin-Tóth M,Frillingos S,Lawrence MC,Kaback HR

    更新日期：2000-05-23 00:00:00

  • Pre-steady-state kinetics of the microtubule-kinesin ATPase.

    abstract：:The pre-steady-state kinetics of the microtubule-kinesin ATPase were investigated by chemical-quench flow methods using the Drosophila kinesin motor domain (K401) expressed in Escherichia coli [Gilbert, S. P., & Johnson, K. A. (1993) Biochemistry 32, 4677-4684]. The results define a minimal mechanism: M.K + ATP in equ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00173a044

    authors： Gilbert SP,Johnson KA

    更新日期：1994-02-22 00:00:00

  • Effect of cholesterol on the membrane interaction of Modelin-5 isoforms.

    abstract：:Modelin-5 isoforms were used to gain an insight into the effects of amidation on antimicrobial selectivity. When tested against Escherichia coli, amidation increased toxicity 10-fold (MIC = 31.25 μM) while showing limited increased hemolytic activity (2% lysis). Our results show that both the amidated and non-amidated...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201267v

    authors： Dennison SR,Phoenix DA

    更新日期：2011-12-20 00:00:00

  • Role of the N-terminal helix I for dimerization and stability of the calcium-binding protein S100B.

    abstract：:Human S100B(beta beta) is a small intracellular EF-hand calcium-binding protein that consists of two noncovalently associated 91-residue beta monomers. The three-dimensional structures of S100B reveal the dimer interface consists of four alpha-helices (I, I' and IV, IV') packed in an X-type bundle. In this study, guan...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0118052

    authors： Ferguson PL,Shaw GS

    更新日期：2002-03-19 00:00:00

  • Hydrogen-deuterium exchange and mass spectrometry reveal the pH-dependent conformational changes of diphtheria toxin T domain.

    abstract：:The translocation (T) domain of diphtheria toxin plays a critical role in moving the catalytic domain across the endosomal membrane. Translocation/insertion is triggered by a decrease in pH in the endosome where conformational changes of T domain occur through several kinetic intermediates to yield a final trans-membr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500893y

    authors： Li J,Rodnin MV,Ladokhin AS,Gross ML

    更新日期：2014-11-04 00:00:00

  • Complete assignment of disulfide bonds in bovine dopamine beta-hydroxylase.

    abstract：:Peptide mapping, chemical sequencing, microbore HPLC/electrospray ionization mass spectrometry (LC/ESI/MS), and matrix-assisted laser desorption mass spectrometry (MALDI/MS) were used to identify the sites of intra- and intermolecular disulfide linkages in bovine dopamine beta-hydroxylase. The enzyme contains 14 cyste...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00204a019

    authors： Robertson JG,Adams GW,Medzihradszky KF,Burlingame AL,Villafranca JJ

    更新日期：1994-09-27 00:00:00

  • Fate of the (2Fe-2S)(2+) cluster of Escherichia coli biotin synthase during reaction: a Mössbauer characterization.

    abstract：:Biotin synthase, the enzyme which catalyzes the last step of the biosynthesis of biotin, contains only (2Fe-2S)(2+) clusters when isolated under aerobic conditions. Previous results showed that reduction by dithionite or photoreduced deazaflavin converts the (2Fe-2S)(2+) to (4Fe-4S)(2+,+). However, until now, no detai...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034426c

    authors： Tse Sum Bui B,Benda R,Schünemann V,Florentin D,Trautwein AX,Marquet A

    更新日期：2003-07-29 00:00:00

  • Localization of the Cl-/HCO3- anion exchanger binding site to the amino-terminal region of carbonic anhydrase II.

    abstract：:Human carbonic anhydrase II (CAII) possesses a binding site for an acidic motif (D887ADD) within the carboxyl-terminal region (Ct) of the human erythrocyte chloride/bicarbonate anion exchanger, AE1. In this study, the amino acid sequence comprising this AE1 binding site was localized to the first 17 residues of CAII, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0015111

    authors： Vince JW,Carlsson U,Reithmeier RA

    更新日期：2000-11-07 00:00:00

  • Regulation of phosphatidate phosphatase activity from the yeast Saccharomyces cerevisiae by phospholipids.

    abstract：:Regulation of Saccharomyces cerevisiae membrane-associated phosphatidate phosphatase (3-sn-phosphatidate phosphohydrolase, EC 3.1.3.4) activity by phospholipids was examined using purified enzyme and Triton X-100/phospholipid-mixed micelles. Anionic phospholipids activated phosphatidate phosphatase activity whereas zw...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi952808f

    authors： Wu WI,Carman GM

    更新日期：1996-03-26 00:00:00

  • The nuclear transport machinery recognizes nucleoplasmin-histone complexes.

    abstract：:The nuclear transport of the chromatin remodeling protein nucleoplasmin and chromatin building histones is mediated by importins. Nucleoplasmin (NP) contains a classical bipartite nuclear localization signal (NLS) that is recognized by the importin α/β heterodimer, while histones present multiple NLS-like motifs that ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi2008867

    authors： Arregi I,Falces J,Bañuelos S,Urbaneja MA,Taneva SG

    更新日期：2011-08-23 00:00:00

  • Hydrolysis of monodisperse phosphatidylcholines by phospholipase A2 occurs on vessel walls and air bubbles.

    abstract：:Hydrolysis of monodisperse short chain phosphatidylcholines, far below their critical micelle concentration, by phospholipase A2 (PLA2) and other interfacial enzymes is characterized. Results show that virtually all the observed hydrolysis by pancreatic and human inflammatory PLA2 occurs on surfaces of the reaction ve...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990194z

    authors： Yu BZ,Berg OG,Jain MK

    更新日期：1999-08-10 00:00:00

  • Tritium isotope effects in adenosylcobalamin-dependent methylmalonyl-CoA mutase.

    abstract：:Methylmalonyl-CoA mutase from Propionibacterium shermanii is an adenosylcobalamin-dependent enzyme which catalyzes the reversible isomerization of methylmalonyl-CoA and succinyl-CoA. The rate of tritium loss from 5'-[3H]adenosylcobalamin during the enzymic reaction and the relative rates of tritium appearance in subst...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961250o

    authors： Meier TW,Thomä NH,Leadlay PF

    更新日期：1996-09-10 00:00:00

  • Conformational changes in aerolysin during the transition from the water-soluble protoxin to the membrane channel.

    abstract：:Proteolytic activation, oligomerization, and membrane insertion are three steps that precede channel formation by the bacterial toxin aerolysin. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and hydrogen-deuterium exchange, the structural changes associated with each step were an...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971216p

    authors： Cabiaux V,Buckley JT,Wattiez R,Ruysschaert JM,Parker MW,van der Goot FG

    更新日期：1997-12-09 00:00:00

  • An appraisal of multiple NADPH binding-site models proposed for cytochrome P450 reductase, NO synthase, and related diflavin reductase systems.

    abstract：:The diflavin reductases exemplified by mammalian cytochrome P450 reductase catalyze NADPH dehydrogenation and electron transfer to an associated monooxygenase. It has recently been proposed that double occupancy of the NADPH dehydrogenation site inhibits the NADPH to FAD hydride transfer step in this series of enzymes...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0360408

    authors： Daff S

    更新日期：2004-04-06 00:00:00

  • Role of the linker region connecting the reductase and heme domains in cytochrome P450BM-3.

    abstract：:Cytochrome P450BM-3 is a fatty acid monooxygenase that contains the catalytic P450 heme domain covalently attached to a diflavin P450 reductase domain. The function of the linker region connecting the C-terminal end of the heme domain to the N-terminal end of the reductase domain has been studied by deleting parts of ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00035a031

    authors： Govindaraj S,Poulos TL

    更新日期：1995-09-05 00:00:00

  • Structure-activity analysis of a Conus peptide blocker of N-type neuronal calcium channels.

    abstract：:The synthetic peptide SNX-111 corresponding to the sequence of the omega-conopeptide MVIIA from the venom of the marine snail Conus magus is a highly potent and selective antagonist of N-type calcium channels. We have synthesized and characterized a large number of analogs of SNX-111 in order to elucidate the structur...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00025a013

    authors： Nadasdi L,Yamashiro D,Chung D,Tarczy-Hornoch K,Adriaenssens P,Ramachandran J

    更新日期：1995-06-27 00:00:00

  • Identification of oxidized amino acid residues in the vicinity of the Mn(4)CaO(5) cluster of Photosystem II: implications for the identification of oxygen channels within the Photosystem.

    abstract：:As a light-driven water-plastoquinone oxidoreductase, Photosystem II produces molecular oxygen as an enzymatic product. Additionally, under a variety of stress conditions, reactive oxygen species are produced at or near the active site for oxygen evolution. In this study, Fourier-transform ion cyclotron resonance mass...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300650n

    authors： Frankel LK,Sallans L,Limbach PA,Bricker TM

    更新日期：2012-08-14 00:00:00