Abstract:
:Biotin synthase, the enzyme which catalyzes the last step of the biosynthesis of biotin, contains only (2Fe-2S)(2+) clusters when isolated under aerobic conditions. Previous results showed that reduction by dithionite or photoreduced deazaflavin converts the (2Fe-2S)(2+) to (4Fe-4S)(2+,+). However, until now, no detailed investigation concerning the fate of the (2Fe-2S)(2+) during reduction under assay conditions (NADPH, flavodoxin, flavodoxin reductase) has been realized. Here, we show by Mössbauer spectroscopy on a partially purified fraction overexpressing the enzyme that, in the presence of a S(2)(-) source and Fe(2+), there is conversion of the predominant (2Fe-2S)(2+) clusters into a 1:1 mixture of (2Fe-2S)(2+) and (4Fe-4S)(2+). No change in this cluster composition was observed in the presence of the physiological reducing system. When the reaction was allowed to proceed by addition of the substrate dethiobiotin, the (4Fe-4S)(2+) was untouched whereas the (2Fe-2S)(2+) was degraded into a new species. This is consistent with the hypothesis that the reduced (4Fe-4S) cluster is involved in mediating the cleavage of AdoMet and that the (2Fe-2S)(2+) is the sulfur source for biotin.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Tse Sum Bui B,Benda R,Schünemann V,Florentin D,Trautwein AX,Marquet Adoi
10.1021/bi034426csubject
Has Abstractpub_date
2003-07-29 00:00:00pages
8791-8issue
29eissn
0006-2960issn
1520-4995journal_volume
42pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::A procedure has been developed to purify foot and mouth disease virus (FMDV) VP1 surface antigens from recombinant Escherichia coli. The VP1 antigens are expressed as fusion proteins derived from the E. coli Trp operon and VP1 surface protein of FMDV. The procedure is capable of recovering greater than 96% of the desi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00321a031
更新日期:1984-12-18 00:00:00
abstract::RalA and RalB are members of the Ras family of small G proteins and are activated downstream of Ras via RalGEFs. The RalGEF-Ral axis represents one of the major effector pathways controlled by Ras and as such is an important pharmacological target. RalA and RalB are approximately 80% identical at the amino acid level;...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi501530u
更新日期:2015-02-17 00:00:00
abstract::The X-ray crystal structure of human soluble epoxide hydrolase (sEH) has been determined at 2.6 A resolution, revealing a domain-swapped quaternary structure identical to that observed for the murine enzyme [Argiriadi, M. A., Morisseau, C., Hammock, B. D., and Christianson, D. W. (1999) Proc. Natl. Acad. Sci. U.S.A. 9...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036189j
更新日期:2004-04-27 00:00:00
abstract::Human melanin-concentrating hormone (hMCH) and many of its analogues are potent but nonspecific ligands for human melanin-concentrating hormone receptors 1 and 2 (hMCH-1R and hMCH-2R). To differentiate between the physiological functions of these receptors, selective antagonists are needed. In this study, analogues of...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0200514
更新日期:2002-05-21 00:00:00
abstract::FixL is a heme-based oxygen-sensing histidine kinase that induces the expression of nitrogen fixation genes under hypoxic conditions. Oxygen dissociation from heme iron in the sensor domain of FixL initiates protein conformational changes that are transmitted to the histidine kinase domain, activating autophosphorylat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00405
更新日期:2016-07-26 00:00:00
abstract::The organization and interaction of chlorophylls (Chl) and the kinetics of the energy transfer in the core antenna of photosystem I (PSI) trimeric and monomeric complexes, isolated from Spirulina platensis with Triton X-100 have been studied by stationary and time-resolved fluorescence. At 295 K both complexes show an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970386z
更新日期:1997-11-11 00:00:00
abstract::The preferential interactions of proteins with solvent components were studied in concentrated aqueous solutions of the sulfate, acetate, and chloride salts of Mg2+, Ba2+, Ca2+, Mn2+ and Ni2+ [except for CaSO4, BaSO4, Mn-(OAc)2, and Ni(OAc)2], and results were compared with those of the Na+ salts. It was found that, f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00320a004
更新日期:1984-12-04 00:00:00
abstract::Vibrational frequencies associated with FeC and CO stretching and FeCO bending modes have been determined via resonance Raman (RR) and infrared (IR) spectroscopy for cytochrome c peroxidase (CCP) mutants prepared by site-directed mutagenesis. These include the bacterial "wild type", CCP(MI), and mutations involving gr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00415a015
更新日期:1988-07-26 00:00:00
abstract::The protein phosphatase 2C (PP2C) family represents one of the four major protein Ser/Thr phosphatase activities in mammalian cells and contains at least 13 distinct gene products. Although PP2C family members regulate a variety of cellular functions, mechanisms of regulation of their activities are largely unknown. H...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800067p
更新日期:2008-07-08 00:00:00
abstract::Cytochrome oxidase proteoliposomes were prepared from bovine heart oxidase. Size distributions determined by quasi-elastic light scattering (QELS) showed that there was a small population of large vesicles (120-200-nm diameter) and a large population of small vesicles (50-100-nm diameter). Trapping cytochrome c inside...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00468a010
更新日期:1990-04-24 00:00:00
abstract::Chemical modification and proteolytic digestion studies have identified a transmembrane glutamic acid residue (E953) of the alpha subunit of the pig kidney Na, K-ATPase as a possible cation binding site [Goldshleger et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 6911-6915]. In addition, an adjacent glutamate (E954) i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00054a012
更新日期:1993-01-26 00:00:00
abstract::The mechanism of bovine intestinal 5'-nucleotide phosphodiesterase was investigated by determining kinetic constants of systematically varied substrates, with emphasis on esters of phosphonic acids (which have much higer Vmax values than conventional phosphodiester substrates), and by pre-steady-state kinetics using b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00625a011
更新日期:1977-03-22 00:00:00
abstract::One of the most fundamental questions in the control of gene expression in mammals is how epigenetic methylation patterns of DNA and histones are established, erased, and recognized. This central process in controlling metazoan gene expression includes coordinated covalent modifications of DNA and its associated histo...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi100213t
更新日期:2010-04-13 00:00:00
abstract::The thermodynamics and kinetics of the subtransition L epsilon----P beta of sonicated unilamellar vesicles of 1-myristoyl-2-stearoylphosphatidylcholine (1M-2S-PC) and of 1-stearoyl-2-myristoylphosphatidylcholine (1S-2M-PC) were studied by equilibrium cooling curves and temperature-jump relaxation spectrometry with an ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00313a009
更新日期:1984-08-28 00:00:00
abstract::Tau is a microtubule associated protein implicated in the pathogenesis of several neurodegenerative diseases. Because of the channel forming properties of other amyloid peptides, we employed planar lipid bilayers and atomic force microscopy to test tau for its ability to form ion permeable channels. Our results demons...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00988
更新日期:2015-12-22 00:00:00
abstract::Phytochromes are widespread red/far-red photosensory proteins well known as critical regulators of photomorphogenesis in plants. It is often assumed that natural selection would have optimized the light sensing efficiency of phytochromes to minimize nonproductive photochemical deexcitation pathways. Surprisingly, the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500108s
更新日期:2014-05-06 00:00:00
abstract::Bacteriophage P22 is a double-stranded DNA containing phage. Its morphogenetic pathway requires the formation of a precursor procapsid that subsequently matures to the capsid. The stability of bacteriophage P22 coat protein in both monomeric and polymeric forms under hydrostatic pressure has been examined previously [...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00004a003
更新日期:1995-01-31 00:00:00
abstract::Nucleolin, also called protein C23, is a RNA-associated protein implicated in the early stages of ribosome assembly. To study the general conformation and map the nucleic acid binding regions, rat nucleolin was subjected to limited proteolysis using trypsin and chymotrypsin in the presence or absence of poly(G). The c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00476a006
更新日期:1990-06-19 00:00:00
abstract::During translation, the small subunit of the ribosome rotates with respect to the large subunit primarily between two states as mRNA is being translated into a protein. At the termination of bacterial translation, class I release factors (RFs) bind to a stop codon in the A-site and catalyze the release of the peptide ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00824
更新日期:2018-04-03 00:00:00
abstract::Monomeric sarcosine oxidase (MSOX) is a prototypical member of a recently recognized family of amine-oxidizing enzymes that all contain covalently bound flavin. Mutation of the covalent flavin attachment site in MSOX produces a catalytically inactive apoprotein (apoCys315Ala) that forms an unstable complex with FAD (K...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0607352
更新日期:2006-08-08 00:00:00
abstract::Inwardly rectifying potassium channels are important in cellular repolarization of many excitable tissues. Amino acid sequence alignment of different mammalian inward rectifier K(+) channels revealed two absolutely conserved cysteine residues in the putative extracellular face, suggesting a possible disulfide bond. Re...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992469g
更新日期:2000-04-25 00:00:00
abstract::Intensive study has been devoted to understanding the kinetic and structural bases underlying the exceptionally high fidelity (low error frequencies) of the typical DNA polymerase. Commonly proposed explanations have included (i) the concept of fidelity check points, in which the correctness of a nascent base pair mat...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi026021i
更新日期:2002-08-27 00:00:00
abstract::The activation of coagulation factor X by tissue factor (TF) and coagulation factor VIIa (VIIa) on a phospholipid surface is thought to be the key step in the initiation of blood coagulation. In this reaction, the product, fXa, is transiently and reversibly bound to the TF-VIIa enzyme complex. This in effect leads to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700136a
更新日期:2007-05-22 00:00:00
abstract::Binding of the coupling ion (Na+ or Li+) and sugars to the purified melibiose permease of Escherichia coli, reconstituted in proteoliposomes, produces selective and cooperative changes of the transporter tryptophan fluorescence. To assess the individual contribution of N- or C-terminal domains of the permease to these...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961372g
更新日期:1996-09-17 00:00:00
abstract::Soluble guanylate cyclase isolated from bovine and rat lung is a heterodimeric hemoprotein composed of alpha1 and beta1 subunits. The heme binding region has been localized to residues 1-385 of the beta1 subunit [beta1(1-385)], while the catalytic site(s) have been localized to the C-terminal region of sGC. There are ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972686m
更新日期:1998-03-31 00:00:00
abstract::Structural properties of heparan sulfate preparations from hog mucosa and beef lung sources were obtained by application of Smith degradation and nitrous acid reactions. Products formed by these reactions indicated that most of the iduronic acid present in these mucopolysaccharides is ester sulfated, whereas N-sulfate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00629a014
更新日期:1977-05-17 00:00:00
abstract::The single-stranded packaged genome (ssDNA) of bacteriophage phi X174 is shown by Raman spectroscopy to lack both the ordered phosphodiester backbone and base stacking, which are demonstrated for unpackaged, protein-free ssDNA. In solutions of moderate ionic strength, unpackaged ssDNA contains 36 +/- 7% of deoxyribosy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00234a004
更新日期:1991-05-21 00:00:00
abstract::In the DNA recognition mode of C(2)H(2)-type zinc fingers, the finger-finger connection region, consisting of the histidine spacing (HX(3-5)H) and linker, would be important for determining the orientation of the zinc finger domains. To clarify the influence of spacing between two ligand histidines in the DNA binding,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035999s
更新日期:2004-05-25 00:00:00
abstract::The structure/function properties of functional groups in the leadzyme have been studied by assaying the activity of analog ribozymes generated by the systematic substitution of modified nucleotides in the internal loop region of the ribozyme. Guanosine analogs introduced at positions 4 and 7 occupied by guanosine in ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962219p
更新日期:1997-03-18 00:00:00
abstract::Here we report that the Methanocaldococcus jannaschii enzyme derived from the MJ0309 gene is an Fe(II) dependent agmatinase (SpeB). This is the first report of an iron-dependent agmatinase. We demonstrate that aerobically isolated recombinant enzyme contains two disulfide bonds and only a trace amount of any metal and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300039f
更新日期:2012-04-10 00:00:00