Fate of the (2Fe-2S)(2+) cluster of Escherichia coli biotin synthase during reaction: a Mössbauer characterization.


:Biotin synthase, the enzyme which catalyzes the last step of the biosynthesis of biotin, contains only (2Fe-2S)(2+) clusters when isolated under aerobic conditions. Previous results showed that reduction by dithionite or photoreduced deazaflavin converts the (2Fe-2S)(2+) to (4Fe-4S)(2+,+). However, until now, no detailed investigation concerning the fate of the (2Fe-2S)(2+) during reduction under assay conditions (NADPH, flavodoxin, flavodoxin reductase) has been realized. Here, we show by Mössbauer spectroscopy on a partially purified fraction overexpressing the enzyme that, in the presence of a S(2)(-) source and Fe(2+), there is conversion of the predominant (2Fe-2S)(2+) clusters into a 1:1 mixture of (2Fe-2S)(2+) and (4Fe-4S)(2+). No change in this cluster composition was observed in the presence of the physiological reducing system. When the reaction was allowed to proceed by addition of the substrate dethiobiotin, the (4Fe-4S)(2+) was untouched whereas the (2Fe-2S)(2+) was degraded into a new species. This is consistent with the hypothesis that the reduced (4Fe-4S) cluster is involved in mediating the cleavage of AdoMet and that the (2Fe-2S)(2+) is the sulfur source for biotin.






Tse Sum Bui B,Benda R,Schünemann V,Florentin D,Trautwein AX,Marquet A




Has Abstract


2003-07-29 00:00:00












  • Self base pairing in a complementary deoxydinucleoside monophosphate duplex: crystal and molecular structure of deoxycytidylyl-(3'-5')-deoxyguanosine.

    abstract::The molecular structure of ammonium deoxycytidylyl-(3'-5')-deoxyguanosine, crystallized from aqueous acetone near pH 4, was determined for X-ray diffraction data. The crystals were tetragonal, space group P43212 with a = b = 11.078 (1) A and c = 45.826 (4) A. The structure was solved by tangent expansion of phases bas...


    pub_type: 杂志文章


    authors: Cruse WB,Egert E,Kennard O,Sala GB,Salisbury SA,Viswamitra MA

    更新日期:1983-04-12 00:00:00

  • Aromatic C-methyltransferases with antipodal stereoselectivity for structurally diverse phenolic amino acids catalyze the methylation step in the biosynthesis of the actinomycin chromophore.

    abstract::The actinomycin biosynthetic gene cluster of Streptomyces chrysomallus harbors two paralogous genes, acmI and acmL, encoding methyltransferases. To unveil their suspected role in the formation of 3-hydroxy-4-methyl-anthranilic acid (4-MHA), the building block of the actinomycin chromophore, each gene was expressed in ...


    pub_type: 杂志文章


    authors: Crnovcić I,Süssmuth R,Keller U

    更新日期:2010-11-16 00:00:00

  • Beta subunit of (Na+ + K+)-ATPase contains three disulfide bonds.

    abstract::Previous studies of titratable (Na+ + K+)-ATPase sulfhydryl groups have indicated the presence of one disulfide bond per mole of holoenzyme. This single disulfide cross-link was assigned to the beta subunit on the basis of the difference between the number of titrated "free" sulfhydryl groups and the total number of t...


    pub_type: 杂志文章


    authors: Miller RP,Farley RA

    更新日期:1990-02-13 00:00:00

  • Membrane topography of the photosynthetic reaction center polypeptides of Rhodopseudomonas sphaeroides.

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    pub_type: 杂志文章


    authors: Bachmann RC,Gillies K,Takemoto JY

    更新日期:1981-08-04 00:00:00

  • Folding and domain-domain interactions of the chaperone PapD measured by 19F NMR.

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    pub_type: 杂志文章


    authors: Bann JG,Frieden C

    更新日期:2004-11-02 00:00:00

  • Prb1 Protease Activity Is Required for Its Recognition by the F-Box Protein Saf1.

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    pub_type: 杂志文章


    authors: Mark KG,Meza-Gutierrez F,Johnson JR,Newton BW,Krogan NJ,Toczyski DP

    更新日期:2015-07-28 00:00:00

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    pub_type: 杂志文章


    authors: Rothery RA,Weiner JH

    更新日期:1991-08-27 00:00:00

  • Mechanism of heparin activation of antithrombin. Evidence for reactive center loop preinsertion with expulsion upon heparin binding.

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    pub_type: 杂志文章


    authors: Huntington JA,Olson ST,Fan B,Gettins PG

    更新日期:1996-07-02 00:00:00

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    pub_type: 杂志文章


    authors: Hartman FC

    更新日期:1981-02-17 00:00:00

  • Equilibrium binding of [3H]tubocurarine and [3H]acetylcholine by Torpedo postsynaptic membranes: stoichiometry and ligand interactions.

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    pub_type: 杂志文章


    authors: Neubig RR,Cohen JB

    更新日期:1979-11-27 00:00:00

  • Introduction of purified hexosaminidase A into Tay-Sachs leukocytes by means of immunoglobulin-coated liposomes.

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    pub_type: 杂志文章


    authors: Cohen CM,Weissmann G,Hoffstein S,Awasthi YC,Srivastava SK

    更新日期:1976-01-27 00:00:00

  • Oxygen infrared spectra of oxyhemoglobins and oxymyoglobins. Evidence of two major liganded O2 structures.

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    pub_type: 杂志文章


    authors: Potter WT,Tucker MP,Houtchens RA,Caughey WS

    更新日期:1987-07-28 00:00:00

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    pub_type: 杂志文章


    authors: Crane EJ 3rd,Parsonage D,Claiborne A

    更新日期:1996-02-20 00:00:00

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    pub_type: 杂志文章


    authors: Barbar E,Barany G,Woodward C

    更新日期:1995-09-12 00:00:00

  • Pentachlorophenol hydroxylase, a poorly functioning enzyme required for degradation of pentachlorophenol by Sphingobium chlorophenolicum.

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Makde RD,Mahajan SK,Kumar V

    更新日期:2007-02-27 00:00:00

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    authors: Roche CJ,Thomson JA,Crothers DM

    更新日期:1994-02-01 00:00:00

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Klein C,Hollender J,Bender H,Schulz GE

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    pub_type: 杂志文章


    authors: Wang Y,Wu C

    更新日期:2017-12-19 00:00:00

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Bradshaw JM,Waksman G

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    更新日期:2009-12-29 00:00:00

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    pub_type: 杂志文章


    authors: Weng W,Li L,van Bennekum AM,Potter SH,Harrison EH,Blaner WS,Breslow JL,Fisher EA

    更新日期:1999-03-30 00:00:00

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    pub_type: 杂志文章


    authors: Kusnetzow A,Dukkipati A,Babu KR,Singh D,Vought BW,Knox BE,Birge RR

    更新日期:2001-07-03 00:00:00

  • Thermodynamics of reversible and irreversible unfolding and domain interactions of glucoamylase from Aspergillus niger studied by differential scanning and isothermal titration calorimetry.

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    pub_type: 杂志文章


    authors: Christensen T,Svensson B,Sigurskjold BW

    更新日期:1999-05-11 00:00:00