当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Dynamic inhomogeneity in the photodynamics of cyanobacterial phytochrome Cph1.

Dynamic inhomogeneity in the photodynamics of cyanobacterial phytochrome Cph1.

Abstract：

:Phytochromes are widespread red/far-red photosensory proteins well known as critical regulators of photomorphogenesis in plants. It is often assumed that natural selection would have optimized the light sensing efficiency of phytochromes to minimize nonproductive photochemical deexcitation pathways. Surprisingly, the quantum efficiency for the forward Pr-to-Pfr photoconversion of phytochromes seldom exceeds 15%, a value very much lower than that of animal rhodopsins. Exploiting ultrafast excitation wavelength- and temperature-dependent transient absorption spectroscopy, we resolve multiple pathways within the ultrafast photodynamics of the N-terminal PAS-GAF-PHY photosensory core module of cyanobacterial phytochrome Cph1 (termed Cph1Δ) that are primarily responsible for the overall low quantum efficiency. This inhomogeneity primarily reflects a long-lived fluorescent subpopulation that exists in equilibrium with a spectrally distinct, photoactive subpopulation. The fluorescent subpopulation is favored at elevated temperatures, resulting in anomalous excited-state dynamics (slower kinetics at higher temperatures). The spectral and kinetic behavior of the fluorescent subpopulation strongly resembles that of the photochemically compromised and highly fluorescent Y176H variant of Cph1Δ. We present an integrated, heterogeneous model for Cph1Δ that is based on the observed transient and static spectroscopic signals. Understanding the molecular basis for this dynamic inhomogeneity holds potential for rational design of efficient phytochrome-based fluorescent and photoswitchable probes.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Kim PW,Rockwell NC,Martin SS,Lagarias JC,Larsen DS

doi

10.1021/bi500108s

subject

Has Abstract

pub_date

2014-05-06 00:00:00

pages

2818-26

issue

17

eissn

0006-2960

issn

1520-4995

journal_volume

53

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Hypertriglyceridemic VLDL decreases plasminogen binding to endothelial cells and surface-localized fibrinolysis.

    abstract：:The effect of normo (NTG)- and hypertriglyceridemic (HTG)-VLDL on cultured human umbilical vein endothelial cell (HUVEC) surface-localized fibrinolysis was examined following pre-incubation with NTG-, HTG-VLDL, LDL (1-20 micrograms/mL) or buffer (control). Ligand binding assays, using 125I-labeled tcu-PA, t-PA, or Glu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi952032i

    authors： Li XN,Koons JC,Benza RL,Parks JM,Varma VK,Bradley WA,Gianturco SH,Taylor KB,Grammer JR,Tabengwa EM,Booyse FM

    更新日期：1996-05-14 00:00:00

  • Structure of the third cytoplasmic loop of bovine rhodopsin.

    abstract：:The three-dimensional high-resolution structure of rhodopsin is unknown, as is the case for almost all integral membrane patients. As part of an alternative approach to determine of membrane protein structure, we are pursuing the structure of cytoplasmic domains of this G-protein receptor. A peptide, rhoIII, with the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00045a002

    authors： Yeagle PL,Alderfer JL,Albert AD

    更新日期：1995-11-14 00:00:00

  • Spatial proximity of the glycine-rich loop and the SH2 thiol in myosin subfragment 1.

    abstract：:Subfragment 1 (S1) prepared from rabbit skeletal muscle myosin was digested with trypsin to cleave the 95K heavy chain into three pieces, i.e., the 23K, 50K, and 20K fragments. The trypsin-treated S1 was then cross-linked with p-nitrophenyl iodoacetate. The cross-linker bridged one of the reactive thiols (SH2) in the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00408a045

    authors： Sutoh K,Hiratsuka T

    更新日期：1988-04-19 00:00:00

  • Structural analysis of G-DNA in solution: A combination of polarized and depolarized dynamic light scattering with hydrodynamic model calculations.

    abstract：:Specific intra- and intermolecular quadruplex conformations of model G-DNA oligonucleotides have been identified from their translational and rotational diffusion coefficients in aqueous solution. The transport properties were determined by polarized and depolarized dynamic light scattering. A comparison with hydrodyn...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990750p

    authors： Bolten M,Niermann M,Eimer W

    更新日期：1999-09-21 00:00:00

  • Characterization and purification of the monoamine transporter of bovine chromaffin granules.

    abstract：:The monoamine transporter of the chromaffin granule membranes can be specifically labeled by the photoaffinity reagent 7-azido-8-[125I]iodoketanserin. The characteristics of the labeled protein have been investigated. Two-dimensional gel electrophoresis of the labeled membranes indicated a MW of about 70,000 and an is...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00122a012

    authors： Isambert MF,Gasnier B,Botton D,Henry JP

    更新日期：1992-02-25 00:00:00

  • Structural analysis of N-linked oligosaccharides of equine chorionic gonadotropin and lutropin beta-subunits.

    abstract：:Equine chorionic gonadotropin (eCG) and lutropin (eLH) are composed of alpha- and beta-subunits with an identical amino acid sequence but show different biological activities. To elucidate the molecular difference between these gonadotropins, the structure of the N-linked oligosaccharides of each beta-subunit was dete...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00251a012

    authors： Matsui T,Mizuochi T,Titani K,Okinaga T,Hoshi M,Bousfield GR,Sugino H,Ward DN

    更新日期：1994-11-29 00:00:00

  • Basic proline-rich proteins from human parotid saliva: complete covalent structures of proteins IB-1 and IB-6.

    abstract：:The complete amino acid sequences of two basic proline-rich proteins, IB-1 and IB-6, from human parotid saliva have been determined. Fragments for sequence analysis were obtained by enzymatic digestions. The proteins have molecular weights of 9571 (IB-1) and 11,530 (IB-6) and contain 34 and 39 mol % proline, respectiv...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00357a013

    authors： Kauffman D,Hofmann T,Bennick A,Keller P

    更新日期：1986-05-06 00:00:00

  • Ultrafast water dynamics at the interface of the polymerase-DNA binding complex.

    abstract：:DNA polymerases slide on DNA during replication, and the interface must be mobile for various conformational changes. The role of lubricant interfacial water is not understood. In this report, we systematically characterize the water dynamics at the interface and in the active site of a tight binding polymerase (pol β...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500810a

    authors： Yang Y,Qin Y,Ding Q,Bakhtina M,Wang L,Tsai MD,Zhong D

    更新日期：2014-08-26 00:00:00

  • Probing receptor binding activity of interleukin-8 dimer using a disulfide trap.

    abstract：:Interleukin-8 (IL-8), a member of the chemokine superfamily, exists as both monomers and dimers, and mediates its function by binding to neutrophil CXCR1 and CXCR2 receptors that belong to the G protein-coupled receptor class. It is now well established that the monomer functions as a high-affinity ligand, but the bin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0605944

    authors： Rajarathnam K,Prado GN,Fernando H,Clark-Lewis I,Navarro J

    更新日期：2006-06-27 00:00:00

  • Nucleotide Excision Repair and Impact of Site-Specific 5',8-Cyclopurine and Bulky DNA Lesions on the Physical Properties of Nucleosomes.

    abstract：:The nonbulky 5',8-cyclopurine DNA lesions (cP) and the bulky, benzo[ a]pyrene diol epoxide-derived stereoisomeric cis- and trans- N2-guanine adducts (BPDE-dG) are good substrates of the human nucleotide excision repair (NER) mechanism. These DNA lesions were embedded at the In or Out rotational settings near the dyad ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b01066

    authors： Shafirovich V,Kolbanovskiy M,Kropachev K,Liu Z,Cai Y,Terzidis MA,Masi A,Chatgilialoglu C,Amin S,Dadali A,Broyde S,Geacintov NE

    更新日期：2019-02-12 00:00:00

  • Isolation and characterization of a dog serum lipoprotein having apolipoprotein A-I as its predominant protein constituent.

    abstract：:The serum high density lipoproteins (HDL) of normolipemic dogs (beagles) were isolated in the density range of p 1.063 to 1.21 g/ml, and characterized in terms of composition and physical properties (flotation and diffusion coefficients, partial specific volume, molecular weight, electrophoretic mobility, ultraviolet ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00654a022

    authors： Edelstein C,Lewis LL,Shainoff JR,Naito H,Scanu AM

    更新日期：1976-05-04 00:00:00

  • Identification of a lipolysis-stimulated receptor that is distinct from the LDL receptor and the LDL receptor-related protein.

    abstract：:This paper provides further characterization of a receptor that, in cells lacking the LDL receptor (FH fibroblasts), mediates lipoprotein binding, uptake, and degradation when incubated with oleate at concentrations not exceeding albumin binding capacity. This oleate-activated receptor is genetically distinct from the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00171a017

    authors： Yen FT,Mann CJ,Guermani LM,Hannouche NF,Hubert N,Hornick CA,Bordeau VN,Agnani G,Bihain BE

    更新日期：1994-02-08 00:00:00

  • Structure of the cytoplasmic loop between putative helices II and III of the mannitol permease of Escherichia coli: a tryptophan and 5-fluorotryptophan spectroscopy study.

    abstract：:In this work, four single tryptophan (Trp) mutants of the dimeric mannitol transporter of Escherichia coli, EII(mtl), are characterized using Trp and 5-fluoroTrp (5-FTrp) fluorescence spectroscopy. The four positions, 97, 114, 126, and 133, are located in a region shown by recent studies to be involved in the mannitol...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi8020668

    authors： Vos EP,Bokhove M,Hesp BH,Broos J

    更新日期：2009-06-16 00:00:00

  • Specific effects of chloride on the photocycle of E194Q and E204Q mutants of bacteriorhodopsin as measured by FTIR spectroscopy.

    abstract：:Low-temperature Fourier transform infrared spectroscopy has been used to study mutants of Glu194 and Glu204, two amino acids that are involved in proton release to the extracellular side of bacteriorhodopsin. Difference spectra of films of E194Q, E204Q, E194Q/E204Q, E9Q/E194Q/E204Q, and E9Q/E74Q/E194Q/E204Q at 243, 27...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi025654u

    authors： Lazarova T,Sanz C,Sepulcre F,Querol E,Padrós E

    更新日期：2002-06-25 00:00:00

  • Crystal structure of a ternary complex of the alcohol dehydrogenase from Sulfolobus solfataricus.

    abstract：:The crystal structure of a ternary complex of the alcohol dehydrogenase from the archaeon Sulfolobus solfataricus (SsADH) has been determined at 2.3 A. The asymmetric unit contains a dimer with a NADH and a 2-ethoxyethanol molecule bound to each subunit. The comparison with the apo structure of the enzyme reveals that...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035271b

    authors： Esposito L,Bruno I,Sica F,Raia CA,Giordano A,Rossi M,Mazzarella L,Zagari A

    更新日期：2003-12-16 00:00:00

  • Pentenediol-Type Compounds Specifically Bind to Voltage-Dependent Anion Channel 1 in Saccharomyces cerevisiae Mitochondria.

    abstract：:Voltage-dependent anion channel 1 (VDAC1) situated in the outer mitochondrial membrane regulates the transfer of various metabolites and is a key player in mitochondria-mediated apoptosis. Although many small chemicals that modulate the functions of VDAC1 have been reported to date, most, if not all, of them cannot be...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b01209

    authors： Unten Y,Murai M,Yamamoto T,Watanabe A,Ichimaru N,Aburaya S,Aoki W,Shinohara Y,Miyoshi H

    更新日期：2019-02-26 00:00:00

  • Three-dimensional structure of N5-carboxyaminoimidazole ribonucleotide synthetase: a member of the ATP grasp protein superfamily.

    abstract：:Escherichia coli PurK, a dimeric N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) synthetase, catalyzes the conversion of 5-aminoimidazole ribonucleotide (AIR), ATP, and bicarbonate to N5-CAIR, ADP, and Pi. Crystallization of both a sulfate-liganded and the MgADP-liganded E. coli PurK has resulted in structures at 2....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991618s

    authors： Thoden JB,Kappock TJ,Stubbe J,Holden HM

    更新日期：1999-11-23 00:00:00

  • Crystal structure of human fibrinogen.

    abstract：:A crystal structure of human fibrinogen has been determined at approximately 3.3 A resolution. The protein was purified from human blood plasma, first by a cold ethanol precipitation procedure and then by stepwise chromatography on DEAE-cellulose. A product was obtained that was homogeneous on SDS-polyacrylamide gels....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi802205g

    authors： Kollman JM,Pandi L,Sawaya MR,Riley M,Doolittle RF

    更新日期：2009-05-12 00:00:00

  • Functional annotation and three-dimensional structure of an incorrectly annotated dihydroorotase from cog3964 in the amidohydrolase superfamily.

    abstract：:The substrate specificities of two incorrectly annotated enzymes belonging to cog3964 from the amidohydrolase superfamily were determined. This group of enzymes are currently misannotated as either dihydroorotases or adenine deaminases. Atu3266 from Agrobacterium tumefaciens C58 and Oant2987 from Ochrobactrum anthropi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi301483z

    authors： Ornelas A,Korczynska M,Ragumani S,Kumaran D,Narindoshvili T,Shoichet BK,Swaminathan S,Raushel FM

    更新日期：2013-01-08 00:00:00

  • Investigating the structural dynamics of α-1,4-galactosyltransferase C from Neisseria meningitidis by nuclear magnetic resonance spectroscopy.

    abstract：:Neisseria meningitidis α-1,4-galactosyltransferase C (LgtC) is responsible for the transfer of α-galactose from donor UDP-galactose to the lipooligosaccharide terminal acceptor lactose. Crystal structures of its substrate analogue complexes have provided key insights into the galactosyl transfer mechanism, including a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi301317d

    authors： Chan PH,Cheung AH,Okon M,Chen HM,Withers SG,McIntosh LP

    更新日期：2013-01-15 00:00:00

  • A quantitative index of substrate promiscuity.

    abstract：:Catalytic promiscuity is a widespread, but poorly understood, phenomenon among enzymes with particular relevance to the evolution of new functions, drug metabolism, and in vitro biocatalyst engineering. However, there is at present no way to quantitatively measure or compare this important parameter of enzyme function...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701448p

    authors： Nath A,Atkins WM

    更新日期：2008-01-08 00:00:00

  • SecA insertion into phospholipids is stimulated by negatively charged lipids and inhibited by ATP: a monolayer study.

    abstract：:SecA-lipid interactions are believed to be important for the translocation of precursor proteins across the inner membrane of Escherichia coli [Lill, R., Dowhan, W., & Wickner, W. (1990) Cell 60, 271-280]. SecA insertion into the phospholipid bilayer could a role in this process. We investigated this possibility by st...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00119a021

    authors： Breukink E,Demel RA,de Korte-Kool G,de Kruijff B

    更新日期：1992-02-04 00:00:00

  • Ternary complex crystal structures of glycogen phosphorylase with the transition state analogue nojirimycin tetrazole and phosphate in the T and R states.

    abstract：:Catalysis by glycogen phosphorylase involves a mechanism in which binding of one substrate tightens the binding of the other substrate to produce a productive ternary enzyme-substrate complex. In this work the molecular basis for this synergism is probed in crystallographic studies on ternary complexes in which the gl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960072w

    authors： Mitchell EP,Withers SG,Ermert P,Vasella AT,Garman EF,Oikonomakos NG,Johnson LN

    更新日期：1996-06-11 00:00:00

  • Homogeneity and variability in the structure of azurin molecules studied by fluorescence decay and circular polarization.

    abstract：:The fluorescence decay of apoazurin derived from Pseudomonas aeruginosa is monoexponential. By this criterion the population of molecules of apoazurin is homogeneous. The emission anisotropy factor and the absorption anisotropy factor at the red edge of the absorption band assume similar values, showing that the trypt...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00680a018

    authors： Grinvald A,Schlessinger J,Pecht I,Steinberg IZ

    更新日期：1975-05-06 00:00:00

  • Properties of membrane-inserted protein kinase C.

    abstract：:Protein kinase C (PKC) interacted with phospholipid vesicles in a calcium-dependent manner and produced two forms of membrane-associated PKC: a reversibly bound form and a membrane-inserted form. The two forms of PKC were isolated and compared with respect to enzyme stability, cofactor requirements, and phorbol ester ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00420a003

    authors： Bazzi MD,Nelsestuen GL

    更新日期：1988-10-04 00:00:00

  • Low-temperature solid-state 13C NMR studies of the retinal chromophore in rhodopsin.

    abstract：:Magic angle sample spinning (MASS) 13C NMR spectra have been obtained of bovine rhodopsin regenerated with retinal prosthetic groups isotopically enriched with 13C at C-5 and C-14. In order to observe the 13C retinal chromophore resonances, it was necessary to employ low temperatures (-15-----35 degrees C) to restrict...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00380a018

    authors： Smith SO,Palings I,Copié V,Raleigh DP,Courtin J,Pardoen JA,Lugtenburg J,Mathies RA,Griffin RG

    更新日期：1987-03-24 00:00:00

  • Protein Arginine Methyltransferase 8: Tetrameric Structure and Protein Substrate Specificity.

    abstract：:Type I protein arginine methyltransferases (PRMTs) catalyze asymmetric dimethylation of various proteins, and their dysregulations often correlate with tumorigenesis or developmental deficiency. Recent studies have focused on the in vivo substrate identification and the enzyme mechanism with peptide substrates. Howeve...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00995

    authors： Lee WC,Lin WL,Matsui T,Chen ES,Wei TY,Lin WH,Hu H,Zheng YG,Tsai MD,Ho MC

    更新日期：2015-12-29 00:00:00

  • Identification of the cyclosporin-binding site in P-glycoprotein.

    abstract：:The binding site of cyclosporin A to P-glycoprotein was characterized by using a multidrug-resistant Chinese hamster ovary cell line. P-glycoprotein photolabeled with diazirine-cyclosporin A analogue was purified by a two-step process involving continuous elution electrophoresis followed by wheat germ agglutinin-agaro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981992c

    authors： Demeule M,Laplante A,Murphy GF,Wenger RM,Béliveau R

    更新日期：1998-12-22 00:00:00

  • Mechanism of the PII-activated phosphatase activity of Escherichia coli NRII (NtrB): how the different domains of NRII collaborate to act as a phosphatase.

    abstract：:The phosphatase activity of the homodimeric NRII protein of Escherichia coli is activated by the PII protein and requires all three domains of NRII. Mutations in the N-terminal domain (L16R), central domain (A129T), C-terminal domain PII-binding site (S227R), and C-terminal domain ATP-lid (Y302N) of NRII result in dim...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi030065p

    authors： Pioszak AA,Ninfa AJ

    更新日期：2003-07-29 00:00:00

  • Role of catalytic residues in enzymatic mechanisms of homologous ketosteroid isomerases.

    abstract：:Ketosteroid isomerase (KSI) is one of the most proficient enzymes catalyzing an allylic isomerization reaction at a diffusion-controlled rate. In this study of KSI, we have detailed the structures of its active site, the role of various catalytic residues, and have explained the origin of the its fast reactivity by ca...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001629h

    authors： Oh KS,Cha SS,Kim DH,Cho HS,Ha NC,Choi G,Lee JY,Tarakeshwar P,Son HS,Choi KY,Oh BH,Kim KS

    更新日期：2000-11-14 00:00:00