Characterization and purification of the monoamine transporter of bovine chromaffin granules.

Abstract:

:The monoamine transporter of the chromaffin granule membranes can be specifically labeled by the photoaffinity reagent 7-azido-8-[125I]iodoketanserin. The characteristics of the labeled protein have been investigated. Two-dimensional gel electrophoresis of the labeled membranes indicated a MW of about 70,000 and an isoelectric point ranging from 3.8 to 4.6. No clear protein spot was associated with the radioactive material, which migrated between glycoproteins GPII and GPIV. The diffuse aspect of the radioactive material indicated a heterogeneity, which was not modified after a second electrophoresis. This heterogeneity was, at least partially, due to glycosylation of the transporter; neuraminidase treatment increased the protein pI up to 6.3, whereas digestion with N-glycopeptidase markedly decreased the apparent MW, from 70,000 to 50,000. SDS-polyacrylamide gel electrophoresis showed that, at low acrylamide concentrations, the labeled material migrated more rapidly than predicted from the mobility of the markers of molecular weight, a behavior which indicated a marked hydrophobicity of the transporter. The labeled protein was purified to homogeneity by a combination of chromatography on DEAE-cellulose at pH 4.5, on immobilized wheat germ agglutinin, and on hydroxylapatite in the presence of SDS. During this purification, the specific radioactivity was increased by a factor of 300-500, with a yield of 10-20%.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Isambert MF,Gasnier B,Botton D,Henry JP

doi

10.1021/bi00122a012

subject

Has Abstract

pub_date

1992-02-25 00:00:00

pages

1980-6

issue

7

eissn

0006-2960

issn

1520-4995

journal_volume

31

pub_type

杂志文章
  • Syntaxin 2 and SNAP-23 are required for regulated surfactant secretion.

    abstract::The secretion of lung surfactant in alveolar type II cells is a complex process involving the fusion of lamellar bodies with the plasma membrane. This process is somewhat different from the exocytosis of hormones and neurotransmitters. For example, it is a relatively slower process, and lamellar bodies are very large ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi036338y

    authors: Abonyo BO,Gou D,Wang P,Narasaraju T,Wang Z,Liu L

    更新日期:2004-03-30 00:00:00

  • Engineered disulfide linking the hinge regions within lactose repressor dimer increases operator affinity, decreases sequence selectivity, and alters allostery.

    abstract::The hinge domain encompasses amino acids 51-60 of lactose repressor (LacI) and plays an important role in its regulatory interaction with operator DNA. This segment makes both hinge-DNA and hinge-hinge' contacts that are critical to DNA binding. Furthermore, this small region serves as a central element in communicati...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0114067

    authors: Falcon CM,Matthews KS

    更新日期:2001-12-25 00:00:00

  • Scaffold/matrix-attached regions act upon transcription in a context-dependent manner.

    abstract::Scaffold/matrix-attached regions (S/MARs) are cis-acting elements with a function outside transcribed regions and in introns. Although they usually augment transcriptional rates, their action is highly context-dependent. We cloned an 800 bp S/MAR element from the upstream border of the human interferon-beta domain at ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi960930o

    authors: Schübeler D,Mielke C,Maass K,Bode J

    更新日期:1996-08-27 00:00:00

  • Bidirectional Transformation of a Metamorphic Protein between the Water-Soluble and Transmembrane Native States.

    abstract::The bidirectional transformation of a protein between its native water-soluble and integral transmembrane conformations is demonstrated for FraC, a hemolytic protein of the family of pore-forming toxins. In the presence of biological membranes, the water-soluble conformation of FraC undergoes a remarkable structural r...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.5b01112

    authors: Tanaka K,Caaveiro JM,Tsumoto K

    更新日期:2015-11-24 00:00:00

  • Proton nuclear magnetic resonance and biochemical studies of oxygenation of human adult hemoglobin in deuterium oxide.

    abstract::The proton nuclear magnetic resonance spectrum of human adult deoxyhemoglobin in D2O in the region from 6 to 20 ppm downfield from the proton resonance of residual water shows a number of hyperfine shifted proton resonances that are due to groups on or near the alpha and beta hemes. The sensitivity of these resonances...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00590a031

    authors: Viggiano G,Ho NT,Ho C

    更新日期:1979-11-13 00:00:00

  • Stabilization of triple-helical nucleic acids by basic oligopeptides.

    abstract::Intermolecular triplex DNA is stabilized by metal cations and polyamines which reduce repulsion between the negatively charged phosphates of the three nucleic acid strands. We use a quantitative chemical-probing assay involving protection of duplex guanines in a homopyrimidine.homopurine (Py.Pu) sequence from dimethyl...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00045a033

    authors: Potaman VN,Sinden RR

    更新日期:1995-11-14 00:00:00

  • Glycosylinositol phospholipid anchors of the scrapie and cellular prion proteins contain sialic acid.

    abstract::The only identified component of the scrapie prion is PrPSc, a glycosylinositol phospholipid (GPI)-linked protein that is derived from the cellular isoform (PrPC) by an as yet unknown posttranslational event. Analysis of the PrPSc GPI has revealed six different glycoforms, three of which are unprecedented. Two of the ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00136a600

    authors: Stahl N,Baldwin MA,Hecker R,Pan KM,Burlingame AL,Prusiner SB

    更新日期:1992-06-02 00:00:00

  • Assembly of A beta amyloid protofibrils: an in vitro model for a possible early event in Alzheimer's disease.

    abstract::Amyloid fibrils comprising primarily the peptides A beta 40 and A beta 42 are a defining feature of the Alzheimer's disease (AD) brain, and convergent evidence suggests that the process of their formation plays a central role in the AD pathogenic pathway. Elucidation of fibril assembly is critical for the discovery of...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9904149

    authors: Harper JD,Wong SS,Lieber CM,Lansbury PT Jr

    更新日期:1999-07-13 00:00:00

  • Phosphoinositide-specific phospholipase C-delta 1: effect of monolayer surface pressure and electrostatic surface potentials on activity.

    abstract::We added phospholipase C-delta 1 (PLC-delta) to the aqueous subphase beneath monolayers formed from mixtures of phosphatidylinositol 4,5-bisphosphate (2% PIP2), phosphatidylserine (33% PS), and phosphatidylcholine (65% PC) and then measured the initial rate of hydrolysis of PIP2 after addition of 10 microM free calciu...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00166a006

    authors: Rebecchi M,Boguslavsky V,Boguslavsky L,McLaughlin S

    更新日期:1992-12-29 00:00:00

  • DNA repair catalyzed by Escherichia coli DNA photolyase containing only reduced flavin: elimination of the enzyme's second chromophore by reduction with sodium borohydride.

    abstract::DNA photolyase from Escherichia coli contains FAD plus a partially characterized, second chromophore. In vivo, the flavin is fully reduced (FADH2), but oxidation to a stable, blue radical (FADH.) occurs during enzyme isolation. The second chromophore is irreversibly reduced by reaction of the enzyme with sodium borohy...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00395a034

    authors: Jorns MS,Wang B,Jordan SP

    更新日期:1987-10-20 00:00:00

  • Effect of surface curvature on stability, thermodynamic behavior, and osmotic activity of dipalmitoylphosphatidylcholine single lamellar vesicles.

    abstract::The size and surface curvature dependence of the properties and stability of single lamellar vesicles have been investigated by using a variety of physicochemical techniques. Dipalmitoylphosphatidylcholine single lamellar vesicles of sizes ranging between 200 and 900 A in diameter have been prepared by the French pres...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00515a024

    authors: Lichtenberg D,Freire E,Schmidt CF,Barenholz Y,Felgner PL,Thompson TE

    更新日期:1981-06-09 00:00:00

  • Either the carboxyl- or the amino-terminal region of the human ecto-ATPase (E-NTPDase 2) confers detergent and temperature sensitivity to the chicken ecto-ATP-diphosphohydrolase (E-NTPDase 8).

    abstract::Human ecto-ATPase (E-NTPDase 2) and chicken ecto-ATP-diphosphohydrolase (E-NTPDase 8) are cell surface nucleotidases with two transmembranous domains, one each at the N- and C-termini. Hydrolysis of substrates occurs in active sites residing in their extracellular domains. Human ecto-ATPase activity is decreased by NP...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi050019k

    authors: Mukasa T,Lee Y,Knowles AF

    更新日期:2005-08-23 00:00:00

  • Structural basis for the quinone reduction in the bc1 complex: a comparative analysis of crystal structures of mitochondrial cytochrome bc1 with bound substrate and inhibitors at the Qi site.

    abstract::Cytochrome bc(1) is an integral membrane protein complex essential to cellular respiration and photosynthesis. The Q cycle reaction mechanism of bc(1) postulates a separated quinone reduction (Q(i)) and quinol oxidation (Q(o)) site. In a complete catalytic cycle, a quinone molecule at the Q(i) site receives two electr...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0341814

    authors: Gao X,Wen X,Esser L,Quinn B,Yu L,Yu CA,Xia D

    更新日期:2003-08-05 00:00:00

  • Arginine 177 is involved in Mn(II) binding by manganese peroxidase.

    abstract::Site-directed mutations R177A and R177K in the gene encoding manganese peroxidase isozyme 1 (mnp1) from Phanerochaete chrysosporium were generated. The mutant enzymes were expressed in P. chrysosporium during primary metabolic growth under the control of the glyceraldehyde-3-phosphate dehydrogenase gene promoter, puri...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi990943c

    authors: Sollewijn Gelpke MD,Moënne-Loccoz P,Gold MH

    更新日期:1999-08-31 00:00:00

  • Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha-subunit from rat brain.

    abstract::Rat brain and kidney cDNA libraries were constructed and screened with a cDNA insert corresponding to the mRNA for the sheep kidney Na+,K+-ATPase catalytic subunit. The alpha-subunit cDNAs isolated from the kidney library were derived from a single class of messenger RNA, and the brain cDNAs were derived from three cl...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00373a001

    authors: Shull GE,Greeb J,Lingrel JB

    更新日期:1986-12-16 00:00:00

  • Acetylthiocholine binds to asp74 at the peripheral site of human acetylcholinesterase as the first step in the catalytic pathway.

    abstract::Studies of ligand binding to acetylcholinesterase (AChE) have demonstrated two sites of interaction. An acyl-enzyme intermediate is formed at the acylation site, and catalytic activity can be inhibited by ligand binding to a peripheral site. The three-dimensional structures of AChE-ligand complexes reveal a narrow and...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi000210o

    authors: Mallender WD,Szegletes T,Rosenberry TL

    更新日期:2000-07-04 00:00:00

  • A study of protein-sodium dodecyl sulfate complexes by transient electric birefringence.

    abstract::The method of transient electric birefringence has been applied to study the conformation of protein-sodium dodecyl sulfate complexes. A model of a deformable prolate ellipsoid has been proposed for the protein-dodecyl sulfate complex. This model is compared to the models proposed by J. A. Reynolds and C. Tanford (197...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00685a030

    authors: Wright AK,Thompson MR,Miller RL

    更新日期:1975-07-15 00:00:00

  • Effect of structural modifications on the activity of the leadzyme.

    abstract::The structure/function properties of functional groups in the leadzyme have been studied by assaying the activity of analog ribozymes generated by the systematic substitution of modified nucleotides in the internal loop region of the ribozyme. Guanosine analogs introduced at positions 4 and 7 occupied by guanosine in ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi962219p

    authors: Chartrand P,Usman N,Cedergren R

    更新日期:1997-03-18 00:00:00

  • Dynamics, energetics, and structure in protein folding.

    abstract::For many decades, protein folding experimentalists have worked with no information about the time scales of relevant protein folding motions and without methods for estimating the height of folding barriers. Protein folding experiments have been interpreted using chemical models in which the folding process is charact...

    journal_title:Biochemistry

    pub_type: 杂志文章,评审

    doi:10.1021/bi060643c

    authors: Naganathan AN,Doshi U,Fung A,Sadqi M,Muñoz V

    更新日期:2006-07-18 00:00:00

  • Conformation of immunoglobulin M. 2. Nanosecond fluorescence depolarization analysis of segmental flexibility in anti-epsilon-l-dimethylamino-5-naphthalenesulfonyl-L-lysine anti-immunoglobulin from horse, pig, and shark.

    abstract::The rotational motions of immunoglobulin M (IgM) were investigated by the nanosecond fluorescence depolarization technique. The fluorophore epsilon-1-dimethylamino-5-naphthalenesulfonyl-L-lysine (DNS-lysine) was specifically bound in the combining sites of anti-DNS IgM antibodies from the horse, pig, and nurse shark. ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00660a033

    authors: Holowka DA,Cathou RE

    更新日期:1976-07-27 00:00:00

  • Primer length dependence of binding of DNA polymerase I Klenow fragment to template-primer complexes containing site-specific bulky lesions.

    abstract::The binding of the benzo[a]pyrene metabolite anti-BPDE (r7, t8-dihydroxy-t9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene) to the N(2) group of 2'-deoxyguanosine residues (dG) is known to adversely affect the Michaelis-Menten primer extension kinetics catalyzed by DNA Pol I and other polymerases. In this work, the impact...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi990614k

    authors: Rechkoblit O,Amin S,Geacintov NE

    更新日期:1999-09-07 00:00:00

  • Sulfated glycoproteins, glycolipids, and glycosaminoglycans from synaptic plasma and myelin membranes: isolation and characterization of sulfated glycopeptides.

    abstract::In this report we provide biochemical evidence that a highly purified synaptic plasma membrane fraction derived from rat brain, after intraventricular injection of 35S-labeled sodium sulfate, is enriched in a number of large sulfated glycoproteins compared with a purified myelin fraction studied concurrently. A fracti...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00670a005

    authors: Simpson DL,Thorne DR,Loch HH

    更新日期:1976-12-14 00:00:00

  • The crystal structure of human procathepsin K.

    abstract::Cathepsin K is a cysteine protease present in human osteoclasts that plays an important role in bone resorption. Cathepsin K is synthesized as an inactive proenzyme and activated under conditions of low pH. Autoproteolytic processing of the N-terminal 99 amino acid propeptide produces the active, mature form of cathep...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9822271

    authors: LaLonde JM,Zhao B,Janson CA,D'Alessio KJ,McQueney MS,Orsini MJ,Debouck CM,Smith WW

    更新日期:1999-01-19 00:00:00

  • Substrate-induced closing of the active site revealed by the crystal structure of pantothenate synthetase from Staphylococcus aureus.

    abstract::Pantothenate synthetase (PS, EC 6.3.2.1) is the last enzyme in the pantothenate biosynthesis pathway, a metabolic pathway identified as a potential target for new antimicrobials. PS catalyzes the ATP-dependent condensation of pantoate and beta-alanine to form pantothenate. Here we report the overexpression, purificati...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi1004206

    authors: Satoh A,Konishi S,Tamura H,Stickland HG,Whitney HM,Smith AG,Matsumura H,Inoue T

    更新日期:2010-08-03 00:00:00

  • Mechanism of the reaction catalyzed by isoaspartyl dipeptidase from Escherichia coli.

    abstract::Isoaspartyl dipeptidase (IAD) is a member of the amidohydrolase superfamily and catalyzes the hydrolytic cleavage of beta-aspartyl dipeptides. Structural studies of the wild-type enzyme have demonstrated that the active site consists of a binuclear metal center positioned at the C-terminal end of a (beta/alpha)(8)-bar...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi050008r

    authors: Martí-Arbona R,Fresquet V,Thoden JB,Davis ML,Holden HM,Raushel FM

    更新日期:2005-05-17 00:00:00

  • Isolation of high-affinity peptide antagonists of 14-3-3 proteins by phage display.

    abstract::The 14-3-3 proteins interact with diverse cellular molecules involved in various signal transduction pathways controlling cell proliferation, transformation, and apoptosis. To aid our investigation of the biological function of 14-3-3 proteins, we have set out to identify high-affinity antagonists. By screening phage ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi991353h

    authors: Wang B,Yang H,Liu YC,Jelinek T,Zhang L,Ruoslahti E,Fu H

    更新日期:1999-09-21 00:00:00

  • Cooperative stabilization of microtubule dynamics by EB1 and CLIP-170 involves displacement of stably bound P(i) at microtubule ends.

    abstract::End binding protein 1 (EB1) and cytoplasmic linker protein of 170 kDa (CLIP-170) are two well-studied microtubule plus-end-tracking proteins (+TIPs) that target growing microtubule plus ends in the form of comet tails and regulate microtubule dynamics. However, the mechanism by which they regulate microtubule dynamics...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi300038t

    authors: Lopus M,Manatschal C,Buey RM,Bjelić S,Miller HP,Steinmetz MO,Wilson L

    更新日期:2012-04-10 00:00:00

  • A structural basis for the regulation of an H-NOX-associated cyclic-di-GMP synthase/phosphodiesterase enzyme by nitric oxide-bound H-NOX.

    abstract::Biofilms are surface-attached communities of bacteria enclosed in a polysaccharide matrix. Bacteria in a biofilm are extremely resistant to antibiotics. Several recent reports have linked the signaling molecule nitric oxide (NO) with biofilm dispersal. We have previously reported that an H-NOX (heme-nitric oxide/oxyge...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi401597m

    authors: Lahiri T,Luan B,Raleigh DP,Boon EM

    更新日期:2014-04-08 00:00:00

  • Phospholipase D2 directly interacts with aldolase via Its PH domain.

    abstract::Mammalian phospholipase D (PLD) has been implicated in the cellular signal transduction pathways leading to diverse physiological events and known to be regulated by many cellular factors. To identify the proteins that interact with PLD, we performed a protein overlay assay with fractions obtained from the sequential ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi015700a

    authors: Kim JH,Lee S,Kim JH,Lee TG,Hirata M,Suh PG,Ryu SH

    更新日期:2002-03-12 00:00:00

  • Role of mixed oxidation states in the oxidation of hemerythrin species by ferricyanide ion.

    abstract::The oxidation of deoxy- and oxyhemerythrin to methemerythrin by Fe(CN)6(3-) has been examined. The first step produces (semi-met)O, a half-oxidized form of hemerythrin which has different spectral and kinetic properties from (semi-met)R which is produced by one-electron reduction of methemerythrin. Further oxidation o...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00559a003

    authors: Bradić Z,Harrington PC,Wilkins RG,Yoneda G

    更新日期:1980-09-02 00:00:00