Abstract：
:The crystal structure of a ternary complex of the alcohol dehydrogenase from the archaeon Sulfolobus solfataricus (SsADH) has been determined at 2.3 A. The asymmetric unit contains a dimer with a NADH and a 2-ethoxyethanol molecule bound to each subunit. The comparison with the apo structure of the enzyme reveals that this medium chain ADH undergoes a substantial conformational change in the apo-holo transition, accompanied by loop movements at the domain interface. The extent of domain closure is similar to that observed for the classical horse liver ADH, although some differences are found which can be related to the different oligomeric states of the enzymes. Compared to its apo form, the SsADH ternary complex shows a change in the ligation state of the active site zinc ion which is no longer bound to Glu69, providing additional evidence of the dynamic role played by the conserved glutamate residue in ADHs. In addition, the structure presented here allows the identification of the substrate site and hence of the residues that are important in the binding of both the substrate and the coenzyme.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Esposito L,Bruno I,Sica F,Raia CA,Giordano A,Rossi M,Mazzarella L,Zagari Adoi
10.1021/bi035271bsubject
Has Abstractpub_date
2003-12-16 00:00:00pages
14397-407issue
49eissn
0006-2960issn
1520-4995journal_volume
42pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Indolethylamine-N-methyltransferase (INMT) is a Class 1 transmethylation enzyme known for its production of N,N-dimethyltryptamine (DMT), a hallucinogen with affinity for various serotonergic, adrenergic, histaminergic, dopaminergic, and sigma-1 receptors. DMT is produced via the action of INMT on the endogenous subst...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500175p
更新日期：2014-05-13 00:00:00
abstract：:The use of derivatives of alpha-thrombin obtained by limited proteolysis, that have only a single peptide bond cleaved, allowed the unequivocal correlation between the change in covalent structure and alteration of the enzymatic properties. beta T-Thrombin contains a single cleavage in the surface loop corresponding t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00406a049
更新日期：1988-03-22 00:00:00
abstract：:Galactosyltransferase (UDPgalactose:glycoprotein galactosyltransferase EC 2.4.1.22) was isolated from swine mesentary lymhromatography on Sepharose 4B colums containing covalently bound p-aminophenyl-beta-D-N-acetylglucosamine. The homogenous enzyme showed a single band on disc gel electrophoresis and had a specific a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00668a009
更新日期：1976-11-16 00:00:00
abstract：:The nuclease hypersensitivity element III(1) (NHE III(1)) of the c-MYC promoter strongly controls the transcriptional activity of the c-MYC oncogene. The purine-rich strand of the NHE III(1) element has been shown to be a silencer element for c-MYC transcription upon formation of a G-quadruplex structure. We have dete...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048242p
更新日期：2005-02-15 00:00:00
abstract：:A 35-amino acid peptide corresponding to the putative "zinc finger" sequence of primase was prepared to study its zinc binding properties. When zinc was added to the peptide, it was found that the fluorescence quantum yield of the single tyrosine increased by 46% and the average lifetime by 34%. The binding stoichiome...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9612778
更新日期：1997-01-21 00:00:00
abstract：:Treatment of cells with the enediyne C-1027 is highly efficient at inducing single- and double-strand DNA breaks. This agent is highly cytotoxic when used at picomolar levels over a period of days. For this study, C-1027 has been used at higher levels for a much shorter time period to look at early cellular responses ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015680c
更新日期：2001-12-04 00:00:00
abstract：:Carbon monoxide dehydrogenase (CODH) from Clostridium thermoaceticum plays a central role in the newly discovered acetyl-CoA pathway [Wood, H.G., Ragsdale, S.W., & Pezacka, E. (1986) FEMS Microbiol. Rev. 39, 345-362]. The enzyme catalyzes the formation of acetyl-CoA from methyl, carbonyl, and CoA groups, and it has sp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00417a045
更新日期：1988-08-23 00:00:00
abstract：:Trimethylamine dehydrogenase (TMADH) from the bacterium Methylophilus methylotrophus (sp. W(3)A(1)) and its C30A mutant were inactivated with three known inactivators of monoamine oxidase, namely, phenylhydrazine, N-cyclopropyl-alpha-methylbenzylamine, and 1-phenylcyclopropylamine. All three compounds irreversibly ina...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0105126
更新日期：2001-07-24 00:00:00
abstract：:We have studied the structure of the CuB site in the binuclear heme-copper center of the fully oxidized form of the quinol-oxidizing cytochrome aa3-600 from Bacillus subtilis by EXAFS and ENDOR spectroscopy. This enzyme is member of the large superfamily of heme-copper respiratory oxidases, which catalyze the reductio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00032a019
更新日期：1995-08-15 00:00:00
abstract：:The two distinct domains of flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase, EC 1.1.2.3) are connected by a typical hinge peptide. To probe the importance of the structural integrity of the hinge region for efficient intraprotein electron transfer, three mutant enzymes have been constructed: H delta 3 [Sharp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi950457z
更新日期：1996-01-23 00:00:00
abstract：:Cysteinyl S-nitrosylation has emerged as an important post-translational modification affecting protein function in health and disease. Great emphasis has been placed on global, unbiased quantification of S-nitrosylated proteins because of physiologic and oxidative stimuli. However, current strategies have been hamper...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200008b
更新日期：2011-06-28 00:00:00
abstract：:Messenger RNA degradation is an important component of overall gene expression. During the final step of eukaryotic mRNA degradation, exoribonuclease 1 (Xrn1) carries out 5' → 3' processive, hydrolytic degradation of RNA molecules using divalent metal ion catalysis. To initiate studies of the 5' → 3' RNA decay machine...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b01035
更新日期：2020-04-21 00:00:00
abstract：:The late intermediates involved in the activation mechanism of bovine rhodopsin are investigated by time-resolved optical absorption spectroscopy. Measurements from 10 μs to 200 ms after photolysis were carried out on membrane suspensions of bovine rhodopsin at a temperature of 15 °C and at pH of 7.3, 8.0, and 8.7. Th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00687
更新日期：2016-09-13 00:00:00
abstract：:Splicing factor RBM10 and its close homologues RBM5 and RBM6 govern the splicing of oncogenes such as Fas, NUMB, and Bcl-X. The molecular architecture of these proteins includes zinc fingers (ZnFs) and RNA recognition motifs (RRMs). Three of these domains in RBM10 that constitute the RNA binding part of this splicing ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b01242
更新日期：2018-03-13 00:00:00
abstract：:Acetohydroxy acid synthase (AHAS) is a thiamin diphosphate (ThDP)-dependent enzyme that catalyzes the first common step in the biosynthesis of branched-chain amino acids, condensation of pyruvate with a second 2-ketoacid to form either acetolactate or acetohydroxybutyrate. AHAS isozyme II from Escherichia coli is spec...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi102051h
更新日期：2011-04-19 00:00:00
abstract：:The reaction between chymotrypsin and N-acetyl-L-phenylalanine p-nitroanilide has been studied at subzero temperatures in fluid aqueous dimethyl sulfoxide solvent. Following initiation of the reaction at temperatures as low as -90 degrees C, a series of four reactions prior to the normal rate-limiting step (acylation)...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00652a031
更新日期：1976-04-06 00:00:00
abstract：:Vibrational Raman spectroscopic experiments have been performed as a function of temperature on aqueous dispersions of synthetic DL-erythro-N-lignoceroylsphingosylphosphocholine [C(24):SPM], a racemic mixture of two highly asymmetric hydrocarbon chain length sphingomyelins. Raman spectral peak-height intensity ratios ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00343a036
更新日期：1985-10-22 00:00:00
abstract：:Ceruloplasmin is unique among the multicopper oxidases in that in addition to the usual copper stoichiometry of one Type 1 copper site and a Type 2/Type 3 trinuclear copper cluster, it contains two other Type 1 sites. This assignment of copper sites, based on copper quantitation, sequence alignment, and crystallograph...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980434v
更新日期：1998-06-30 00:00:00
abstract：:The nitrite anion is known to oxidize and degrade hemoglobin (Hb). Recent literature reports suggest a nitrite reductase activity for Hb, converting nitrite into nitric oxide. Surprisingly, no structural information about Hb-nitrite interactions has been reported. We have determined the crystal structure of the ferric...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801015c
更新日期：2008-08-12 00:00:00
abstract：:The binding of the codon UUC to the isolated anticodon loop of tRNAPhe (yeast) has been studied as a model of codon recognition by a simple adaptor. Fluorescence titrations demonstrate that UUC binds to the isolated anticodon loop with an equilibrium constant of 1.4 X 10(3) M-1 (at 7.2 degrees C). Equilibrium sediment...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00369a005
更新日期：1986-10-21 00:00:00
abstract：:Soybean lipoxygenase 1 was studied using limited proteolysis and active-site labeling to begin the structural characterization of the enzyme in solution. The serine proteases trypsin and chymotrypsin cleaved the large monomeric protein (95 kDa) into two large polypeptides, a C-terminal fragment of about 30 kDa and an ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00148a034
更新日期：1992-08-25 00:00:00
abstract：:The proteasome inhibitors, lactacystin and N-acetyl-leucyl-leucyl-norlucinal, caused a rapid and near-complete loss of approximately 22-23-kDa ubiquitinated nucleoproteins, which we have identified as monoubiquitinated nucleosomal histones H2A and H2B by immunological and two-dimensional electrophoretic techniques. In...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970998j
更新日期：1997-11-25 00:00:00
abstract：:The folding of the peptide chain of the bovine heart oxoglutarate carrier in the inner mitochondrial membrane and in the membrane of reconstituted proteoliposomes has been investigated by enzymatic and immunochemical approaches using proteinase K and polyclonal site-directed antibodies, respectively. Two peptides corr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00178a030
更新日期：1994-03-29 00:00:00
abstract：:The light-driven enzyme NADPH:protochlorophyllide oxidoreductase (POR) catalyzes the reduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide), a key regulatory step in the chlorophyll biosynthesis pathway. As POR is light activated, it allows intermediates in the reaction pathway to be observed by initiat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0268448
更新日期：2003-01-21 00:00:00
abstract：:The bacterial DNA cytosine methyltransferase M.HhaI sequence-specifically modifies DNA in an S-adenosylmethionine dependent reaction. The enzyme stabilizes the target cytosine (GCGC) into an extrahelical position, with a concomitant large movement of an active site loop involving residues 80-99. We used multidimension...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi602662e
更新日期：2007-06-19 00:00:00
abstract：:The crystal structure of the ternary complex citrate synthase-oxaloacetate-carboxymethyl coenzyme A has been solved to a resolution of 1.9 A and refined to a conventional crystallographic R factor of 0.185. The structure resembles a proposed transition state of the condensation reaction and suggests that the condensat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：
更新日期：1990-03-06 00:00:00
abstract：:The properties of a series of new potentiometric membrane probes have been explored. The probes all contain an (aminostyryl)pyridinium chromophore or a more highly conjugated analogue. The spectral properties of the dyes are discussed in terms of the excitation-induced charge shift from the pyridine to the aniline; th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00342a010
更新日期：1985-10-08 00:00:00
abstract：:The location of the sites of methylation of the methyl-accepting chemotaxis proteins (MCP) of Escherichia coli has been investigated by trypsin digestion and mapping of the peptides. The two principal MCPs, MCP I and MCP II, were found to have very similar methyl-labeled peptide patterns. Each produced three major spe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00507a051
更新日期：1981-02-17 00:00:00
abstract：:The structure of PsbQ, one of the three main extrinsic proteins associated with the oxygen-evolving complex (OEC) of higher plants and green algae, is examined by Fourier transform infrared (FTIR) and circular dichroic (CD) spectroscopy and by computational structural prediction methods. This protein, together with tw...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026575l
更新日期：2003-02-04 00:00:00
abstract：:To probe the details of protein heme interactions, we have developed a Raman difference spectroscopic technique, which allows reliable detection of very small, approximately equal to 0.01 cm-1, frequency differences. When this technique is applied to heme proteins, structural differences in the protein which perturb t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00525a030
更新日期：1981-10-27 00:00:00
