Proposed mechanism for the condensation reaction of citrate synthase: 1.9-A structure of the ternary complex with oxaloacetate and carboxymethyl coenzyme A.

abstract：:The two key structural features of alpha 2-macroglobulin (alpha 2M) involved in inhibitory caging of proteases are the thiol ester and the bait region. This paper examines the environment of the hydrolyzed thiol ester in methylamine-treated human alpha 2M and the separation between the bait region and the thiol ester ...

journal_title：Biochemistry

authors： Gettins P,Beth AH,Cunningham LW

更新日期：1988-04-19 00:00:00

abstract：:Modelin-5 isoforms were used to gain an insight into the effects of amidation on antimicrobial selectivity. When tested against Escherichia coli, amidation increased toxicity 10-fold (MIC = 31.25 μM) while showing limited increased hemolytic activity (2% lysis). Our results show that both the amidated and non-amidated...

journal_title：Biochemistry

authors： Dennison SR,Phoenix DA

更新日期：2011-12-20 00:00:00

abstract：:While within a human host the opportunistic pathogen Toxoplasma gondii relies heavily on glycolysis for its energy needs. Lactate dehydrogenase (LDH), the terminal enzyme in anaerobic glycolysis necessary for NAD(+) regeneration, therefore represents an attractive therapeutic target. The tachyzoite stage lactate dehyd...

journal_title：Biochemistry

authors： Kavanagh KL,Elling RA,Wilson DK

更新日期：2004-02-03 00:00:00

abstract：:Cell-free extracts from the HTl clone of cultured Chinese hamster lung cells efficiently promote the incorporation of proline into newly synthesized material, 50% of which is digestible to small peptides by highly purified bacterial collagenase. The synthesis of the these products occurs under optimal protein synthesi...

journal_title：Biochemistry

authors： Haralson MA,Frey KL,Mitchell WM

更新日期：1978-03-07 00:00:00

abstract：:Intrinsic disorder is at the center of biochemical regulation and is particularly overrepresented among the often multifunctional viral proteins. Replication and transcription of the respiratory syncytial virus (RSV) relies on a RNA polymerase complex with a phosphoprotein cofactor P as the structural scaffold, which ...

journal_title：Biochemistry

authors： Noval MG,Esperante SA,Molina IG,Chemes LB,Prat-Gay Gd

更新日期：2016-03-15 00:00:00

abstract：:By suspending bovine rhodopsin in trehalose-water glass films, it is possible to trap photostates in the light-activation process. Because of the unusually high vitrification temperature of trehalose-water mixtures, this trapping can be accomplished at room temperature. This allows for a facile investigation of the sp...

journal_title：Biochemistry

authors： Sikora S,Little AS,Dewey TG

更新日期：1994-04-19 00:00:00

abstract：:We have investigated the effects of mixed Na+: Mg2+ ionic solutions on the stability of the nonspecific lac repressor-DNA complex. The effects of Mg2+ are simply interpreted in terms of its role as a competitor (with repressor) for DNA sites. From these studies, the binding constant of the Mg-DNA complex can be determ...

journal_title：Biochemistry

authors： Record MT Jr,deHaseth PL,Lohman TM

更新日期：1977-11-01 00:00:00

abstract：:Unoccupied estrogen receptor (ER) can be extracted from tissues by homogenization with a hypotonic buffer, whereas hormone-occupied ER becomes tightly bound to the nuclear pellet and must be extracted with high-salt-containing buffers. The molecular basis for estrogen-induced tight nuclear binding of ER remains an imp...

journal_title：Biochemistry

authors： Fritsch M,Aluker M,Murdoch FE

更新日期：1999-06-01 00:00:00

abstract：:Escherichia coli asparagine synthetase B (AS-B) catalyzes the synthesis of asparagine from aspartate, glutamine, and ATP. A combination of kinetic, isotopic-labeling, and stoichiometry studies have been performed to define the nature of nitrogen transfer mediated by AS-B. The results of initial rate studies were consi...

journal_title：Biochemistry

authors： Boehlein SK,Stewart JD,Walworth ES,Thirumoorthy R,Richards NG,Schuster SM

更新日期：1998-09-22 00:00:00

abstract：:The reactions of fully reduced and mixed-valence bovine heart cytochrome c oxidase with dioxygen have been reinvestigated in the absence and presence of metal ions (Zn(2+), Ni(2+), and Cd(2+)) by time-resolved optical absorption spectroscopy using the CO flow-flash technique. The time-resolved data were recorded on a ...

journal_title：Biochemistry

authors： Szundi I,Cappuccio J,Einarsdóttir O

更新日期：2004-12-21 00:00:00

abstract：:When they are translated, steroid receptors are assembled into a multiprotein complex containing hsp90, p23, an immunophilin, and often some hsp70. Some of the receptors, such as that for progesterone, have nuclear localization signals that are functional in the absence of hormone, and they move into the nucleus where...

journal_title：Biochemistry

authors： Czar MJ,Galigniana MD,Silverstein AM,Pratt WB

更新日期：1997-06-24 00:00:00

abstract：:The flavin-dependent quiescin-sulfhydryl oxidase (QSOX) inserts disulfide bridges into unfolded reduced proteins with the reduction of molecular oxygen to form hydrogen peroxide. This work investigates how QSOX and protein disulfide isomerase (PDI) cooperate in vitro to generate native pairings in two unfolded reduced...

journal_title：Biochemistry

authors： Rancy PC,Thorpe C

更新日期：2008-11-18 00:00:00

abstract：:Direct chemical titrations of reduced, purified cytochrome aa3 were monitored at 604 nm. Anaerobic oxidation by potassium ferricyanide or 1,1'-bis(hydroxymethyl)-ferricinium ion was compared with the natural substrate, molecular oxygen. The four electrons from reduced cytochrome aa3 were donated to one oxygen molecule...

journal_title：Biochemistry

authors： Schroedl NA,Hartzell CR

更新日期：1977-04-05 00:00:00

abstract：:The interaction of solvent of the substrate binding domain of the bacterial heat shock 70 chaperone protein DnaK was studied in its apo form and with bound hydrophobic substrate peptide, using refined nuclear magnetic resonance experiments. Distinct differences between the two states of the protein were observed. Acco...

journal_title：Biochemistry

authors： Cai S,Stevens SY,Budor AP,Zuiderweg ER

更新日期：2003-09-30 00:00:00

abstract：:Locked nucleic acid (LNA) is a nucleic acid analogue containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation. LNA oligonucleotides display unprecedented hybridization affinity toward complementary single-stranded RNA and complementary single- or double...

journal_title：Biochemistry

authors： Vester B,Wengel J

更新日期：2004-10-26 00:00:00

abstract：:ATP-dependent Mg2+ accumulation in isolated mitochondria occurs predominantly in the matrix and inner membrane compartments. In mitochondria contaminated with lysosomes, the time course and magnitude of ATP-dependent Mg2+ accumulation are influenced by various cytoplasmic substances, besides substrates of the citric a...

journal_title：Biochemistry

authors： Kun E

更新日期：1976-06-01 00:00:00

abstract：:The members of the glutamine amidotransferase (GATase) family catalyze the incorporation of ammonia within numerous metabolic pathways and can be categorized in two classes. Here, we concentrated on class I GATases, which are heteromeric enzyme complexes consisting of synthase subunits and glutaminase subunits with a ...

journal_title：Biochemistry

authors： Semmelmann F,Hupfeld E,Heizinger L,Merkl R,Sterner R

更新日期：2019-06-04 00:00:00

abstract：:Transient contacts between denatured polypeptide chains are likely to play an important part in the initial stages of protein aggregation and fibrillation. To analyze the nature of such contacts, we have carried out a protein engineering study of the 102-residue protein U1A, which aggregates transiently in the wild-ty...

journal_title：Biochemistry

authors： Otzen DE,Miron S,Akke M,Oliveberg M

更新日期：2004-10-19 00:00:00

abstract：:The Z and E isomers of 6-(methoxymethylene)-penicillanic acid have been synthesized, and their interaction with the RTEM beta-lactamase has been studied. The Z isomer is an inhibitor and an inactivator of the enzyme, and there is some similarity between its behavior and that of other mechanism-based inactivators such ...

journal_title：Biochemistry

authors： Brenner DG,Knowles JR

更新日期：1984-11-20 00:00:00

abstract：:Peptidyl derivatives of diphenyl (alpha-aminoalkyl)phosphonates have been synthesized and are effective and specific inhibitors of serine proteases at low concentrations. Z-PheP(OPh)2 irreversibly reacts with chymotrypsin (kobsd/[I] = 1200 M-1 s-1) and does not react with two elastases. The best inhibitor for most chy...

journal_title：Biochemistry

authors： Oleksyszyn J,Powers JC

更新日期：1991-01-15 00:00:00

abstract：:Activator of G protein signaling 3 (AGS3) activates the Gbetagamma mating pathway in yeast in a manner that is independent of heptahelical receptors. It competes with Gbetagamma subunits to bind GDP-bound Gi/o(alpha) subunits via four repeated G protein regulatory (GPR) domains in the carboxyl-terminal half of the mol...

journal_title：Biochemistry

authors： Ma H,Peterson YK,Bernard ML,Lanier SM,Graber SG

更新日期：2003-07-08 00:00:00

abstract：:Remodeling of photosynthetic machinery induced by growing spinach plants under low light intensities reveals an up-regulation of light-harvesting complexes and down-regulation of photosystem II and cytochrome b6f complexes in intact thylakoids and isolated grana membranes. The antenna size of PSII increased by 40-60% ...

journal_title：Biochemistry

authors： Kirchhoff H,Haase W,Wegner S,Danielsson R,Ackermann R,Albertsson PA

更新日期：2007-10-02 00:00:00

abstract：:Following a 1-h [3H]uridine pulse of cells of a human colon carcinoma line, 15% of the radioactivity in heterogeneous nuclear RNA associated with both chromatin and nuclear ribonucleoprotein was not digested to acid soluble fragments during a 2-h incubation with staphylococcal nuclease (EC 3.1.4.7). These [3H]uridine-...

journal_title：Biochemistry

authors： Augenlicht LH,McCormick M,Lipkin M

更新日期：1976-08-24 00:00:00

abstract：:Decapping is the first committed step in 5'-to-3' RNA decay, and in the cytoplasm of human cells, multiple decapping enzymes regulate the stabilities of distinct subsets of cellular transcripts. However, the complete set of RNAs regulated by any individual decapping enzyme remains incompletely mapped, and no consensus...

journal_title：Biochemistry

authors： Luo Y,Schofield JA,Simon MD,Slavoff SA

更新日期：2020-11-03 00:00:00

abstract：:Light-induced activation of the LOV2-Jα domain of the photoreceptor phototropin from oat is believed to involve the detachment of the Jα helix from the central β-sheet and its subsequent unfolding. The dynamics of these conformational changes were monitored by time-resolved emission spectroscopy with 100 ns time resol...

journal_title：Biochemistry

authors： Hoersch D,Bolourchian F,Otto H,Heyn MP,Bogomolni RA

更新日期：2010-12-28 00:00:00

abstract：:The sensitivity of the fluorescence energy of 2-p-toluidinylnaphthalene-6-sulfonate (TNS) to apparent solvent polarity is considerably greater in methanol-water mixtures and lower primary alcohols than in higher primary alcohols or solvents containing no hydroxyl groups. It is suggested that the behavior of TNS in low...

journal_title：Biochemistry

authors： Greene FC

更新日期：1975-02-25 00:00:00

abstract：:The present study was undertaken for the purpose of evaluating the solvent perturbation technique as a method to identify enzyme catalytic residues. For establishment of expected directions and sizes of pKa perturbations for different types of acids in different classes of solvents, a study of the pKa of a series of a...

journal_title：Biochemistry

authors： Grace S,Dunaway-Mariano D

更新日期：1983-08-30 00:00:00

abstract：:Novel inhibitors of lupin diadenosine 5',5'''-P(1),P(4)-tetraphosphate (Ap(4)A) hydrolase have been identified by in silico screening of a large virtual chemical library. Compounds were ranked on the basis of a consensus from six scoring functions. From the top 100 ranked compounds six were selected and initially scre...

journal_title：Biochemistry

authors： Branson KM,Mertens HD,Swarbrick JD,Fletcher JI,Kedzierski L,Gayler KR,Gooley PR,Smith BJ

更新日期：2009-08-18 00:00:00

abstract：:P-Glycoprotein (Pgp) expression in cell lines derived from tumors arising from cells which normally express Pgp can be increased by sodium butyrate and other differentiating agents. Although the Pgp level increased 25-fold after sodium butyrate treatment in SW620 human colon carcinoma cells, the intracellular accumula...

journal_title：Biochemistry

authors： Bates SE,Currier SJ,Alvarez M,Fojo AT

更新日期：1992-07-21 00:00:00

abstract：:The role of reversible phosphorylation in histamine-induced Ca2+ oscillations in HeLa cells has been investigated by using various activators and inhibitors of protein kinases and phosphatases. Electroporation was employed to introduce impermeable materials into single cells, which proved to be a useful and convenient...

journal_title：Biochemistry

authors： Zhu DM,Tekle E,Chock PB,Huang CY

更新日期：1996-06-04 00:00:00