Kinetics of ATP-dependent Mg2+ flux in mitochondria.

abstract：:With knowledge of individual transfer free energies of chemical groups that become newly exposed on protein denaturation and assuming the group transfer free energy contributions are additive, it should be possible to predict the stability of a protein in the presence of denaturant. Unfortunately, several unresolved i...

journal_title：Biochemistry

authors： Auton M,Bolen DW

更新日期：2004-02-10 00:00:00

abstract：:Human carbonic anhydrase II (CAII) possesses a binding site for an acidic motif (D887ADD) within the carboxyl-terminal region (Ct) of the human erythrocyte chloride/bicarbonate anion exchanger, AE1. In this study, the amino acid sequence comprising this AE1 binding site was localized to the first 17 residues of CAII, ...

journal_title：Biochemistry

authors： Vince JW,Carlsson U,Reithmeier RA

更新日期：2000-11-07 00:00:00

abstract：:DNA polymerase (pol) β is a small eukaryotic DNA polymerase composed of two domains. Each domain contributes an enzymatic activity (DNA synthesis and deoxyribose phosphate lyase) during the repair of simple base lesions. These domains are termed the polymerase and lyase domains, respectively. Pol β has been an excelle...

journal_title：Biochemistry

authors： Beard WA,Wilson SH

更新日期：2014-05-06 00:00:00

abstract：:NMR studies of protein denatured states provide insights into potential initiation sites for folding that may be too transient to be observed kinetically. We have characterized the structure and dynamics of the acid-denatured state of protein G by using a F30H mutant of G(B1) which is on the margin of stability. At 5 ...

journal_title：Biochemistry

authors： Sari N,Alexander P,Bryan PN,Orban J

更新日期：2000-02-08 00:00:00

abstract：:The beta chain 15-42 sequence of the fibrin(ogen) E region was implicated in heparin binding [Odrljin et al. (1996) Blood 88, 2050-2061]; whether heparin binds to other fibrin(ogen) regions remains to be clarified. To address this question, we studied the interaction of heparin with fibrinogen, fibrin, and their major...

journal_title：Biochemistry

authors： Yakovlev S,Gorlatov S,Ingham K,Medved L

更新日期：2003-07-01 00:00:00

abstract：:In order to define structural regions in thrombin that interact with hirudin, the N alpha-dinitrofluorobenzyl analogue of an undecapeptide was synthesized corresponding to residues 54-64 of hirudin [GDFEEIPEEY(O35SO3)L (DNFB-[35S]Hir54-64)]. DNFB-[35S]Hir54-64 was reacted at a 10-fold molar excess with human alpha-thr...

journal_title：Biochemistry

authors： Bourdon P,Fenton JW 2nd,Maraganore JM

更新日期：1990-07-10 00:00:00

abstract：:A conserved, crystallographically defined bile acid binding site was originally identified in the membrane domain of mammalian and bacterial cytochrome c oxidase (CcO). Current studies show other amphipathic molecules including detergents, fatty acids, steroids, and porphyrins bind to this site and affect the already ...

journal_title：Biochemistry

authors： Hiser C,Buhrow L,Liu J,Kuhn L,Ferguson-Miller S

更新日期：2013-02-26 00:00:00

abstract：:This work shows that the voltage across membranes in two very different preparations, lipid vesicles in suspension and individual HeLa cells under a microscope, is linearly related to the ratio of fluorescence excited from the two wings of the absorption spectrum of a voltage-sensitive dye. The dye di-4-ANEPPS [1-(3-s...

journal_title：Biochemistry

authors： Montana V,Farkas DL,Loew LM

更新日期：1989-05-30 00:00:00

abstract：:In this communication, we present data which describe optimum conditions for reverse transcription of large ribonucleic acid (RNA) templates into deoxyribonucleic acid (DNA) transcripts by the avian retrovirus reverse transcriptase in vitro. In contrast to previous studies, we have optimized all of the reaction compon...

journal_title：Biochemistry

authors： Retzel EF,Collett MS,Faras AJ

更新日期：1980-02-05 00:00:00

abstract：:The pK(a) values measured previously for the internal Lys-66, Asp-66, and Glu-66 in variants of a highly stable form of staphylococcal nuclease are shifted by as many as 5 pK(a) units relative to normal pK(a) values in water. These shifts cannot be reproduced with continuum electrostatics calculations with static stru...

journal_title：Biochemistry

authors： Karp DA,Stahley MR,García-Moreno B

更新日期：2010-05-18 00:00:00

abstract：:GlgE (EC 2.4.99.16) is an α-maltose 1-phosphate:(1→4)-α-d-glucan 4-α-d-maltosyltransferase of the CAZy glycoside hydrolase 13_3 family. It is the defining enzyme of a bacterial α-glucan biosynthetic pathway and is a genetically validated anti-tuberculosis target. It catalyzes the α-retaining transfer of maltosyl units...

journal_title：Biochemistry

authors： Syson K,Stevenson CE,Rashid AM,Saalbach G,Tang M,Tuukkanen A,Svergun DI,Withers SG,Lawson DM,Bornemann S

更新日期：2014-04-22 00:00:00

abstract：:Proteorhodopsins are a recently discovered class of microbial rhodopsins, ubiquitous in marine bacteria. Over 4000 variants have thus far been discovered, distributed throughout the oceans of the world. Most variants fall into one of two major groups, green- or blue-absorbing proteorhodopsin (GPR and BPR, respectively...

journal_title：Biochemistry

authors： Kralj JM,Bergo VB,Amsden JJ,Spudich EN,Spudich JL,Rothschild KJ

更新日期：2008-03-18 00:00:00

abstract：:Bypass synthesis by DNA polymerase I was studied using synthetic 40-nucleotide-long gapped duplex DNAs each containing a site-specific abasic site analog, as a model system for mutagenesis associated with DNA lesions. Bypass synthesis proceeded in two general stages: a fast polymerization stage that terminated opposit...

journal_title：Biochemistry

authors： Paz-Elizur T,Takeshita M,Livneh Z

更新日期：1997-02-18 00:00:00

abstract：:Ultraviolet irradiation of calmodulin in the presence of calcium results in either the intramolecular cross-linking of Tyr99 and Tyr138 [Malencik, D.A., & Anderson, S.R. (1986) Biochemistry 25, 709] or, when [Tyr8]substance P is bound, the generation of peptide-calmodulin adducts. The latter consist of two chromatogra...

journal_title：Biochemistry

authors： Malencik DA,Anderson SR

更新日期：1988-02-09 00:00:00

abstract：:To identify the specific component(s) in the target membrane involved in fusion of vesicular stomatitis virus (VSV), we examined the interaction of the virus with human erythrocyte membranes with asymmetric and symmetric bilayer distributions of phospholipids. Fusion was monitored spectrofluorometrically by the octade...

journal_title：Biochemistry

authors： Herrmann A,Clague MJ,Puri A,Morris SJ,Blumenthal R,Grimaldi S

更新日期：1990-05-01 00:00:00

abstract：:Optical detection of triplet-state magnetic resonance (ODMR) is employed to study the complexes formed between gene 32 protein (GP32), a single-stranded DNA-binding protein from bacteriophage T4, and the heavy-atom-derivatized polynucleotides poly(5-HgU) and poly(5-BrU). The triplet-state properties of some of the try...

journal_title：Biochemistry

authors： Khamis MI,Maki AH

更新日期：1986-10-07 00:00:00

abstract：:LTbetaR is a member of the TNF receptor family of proteins. It binds to two different cell surface ligands, LIGHT, a homotypic trimer, and LTalpha1beta2, a heterotypic trimer. We have measured the affinities of the dimeric IgG fusion protein, LTbetaRIgG, and monomeric LTbetaR protein binding to both LIGHT and LTalpha1...

journal_title：Biochemistry

authors： Eldredge J,Berkowitz S,Corin AF,Day ES,Hayes D,Meier W,Strauch K,Zafari M,Tadi M,Farrington GK

更新日期：2006-08-22 00:00:00

abstract：:13C NMR has been used to observe the equilibrium complex of [5,5-2H,5-13C]-5-aminolevulinate [( 5,5-2H,5-13C]ALA) bound to porphobilinogen (PBG) synthase (5-aminolevulinate dehydratase), a 280,000-dalton protein. [5,5-2H,5-13C]ALA (chemical shift 46.9 ppm in D2O) was prepared from [5-13C]ALA through enolization in deu...

journal_title：Biochemistry

authors： Jaffe EK,Markham GD

更新日期：1988-06-14 00:00:00

abstract：:It has been suggested that dimeric tubulin can participate in the signal transduction process through its association with the GTP-binding (G) proteins Gs and Gi1. Using the photoaffinity GTP analog, azidoanilido-GTP, it has been shown that the transfer of nucleotide from tubulin to G alpha s and G alpha i1 is the key...

journal_title：Biochemistry

authors： Roychowdhury S,Rasenick MM

更新日期：1994-08-16 00:00:00

abstract：:We consider the effects that different lipid surfaces have upon the denaturation and subsequent formation of amyloid fibrils of bovine insulin. The adsorption and unfolding kinetics of insulin being adsorbed onto the different lipid surfaces under denaturing conditions are studied using FTIR ATR spectroscopy and are c...

journal_title：Biochemistry

authors： Sharp JS,Forrest JA,Jones RA

更新日期：2002-12-31 00:00:00

abstract：:Mutation of Pro94 to phenylalanine or alanine significantly alters the redox properties of the type I copper center of amicyanin. Each mutation increases the redox midpoint potential (E(m)) value by at least 140 mV and shifts the pK(a) for the pH dependence of the E(m) value to a more acidic value. Atomic resolution (...

journal_title：Biochemistry

authors： Carrell CJ,Sun D,Jiang S,Davidson VL,Mathews FS

更新日期：2004-07-27 00:00:00

abstract：:The recruitment and organization of clathrin at endocytic sites first to form coated pits and then clathrin-coated vesicles depend on interactions between the clathrin N-terminal domain (TD) and multiple clathrin binding sequences on the cargo adaptor and accessory proteins that are concentrated at such sites. Up to f...

journal_title：Biochemistry

authors： Zhuo Y,Cano KE,Wang L,Ilangovan U,Hinck AP,Sousa R,Lafer EM

更新日期：2015-04-28 00:00:00

abstract：:There has been intense interest in the development of factor Xa inhibitors for the treatment of thrombotic diseases. Our laboratory has developed a series of novel non-amidine inhibitors of factor Xa. This paper presents two crystal structures of compounds from this series bound to factor Xa. The first structure is de...

journal_title：Biochemistry

authors： Adler M,Kochanny MJ,Ye B,Rumennik G,Light DR,Biancalana S,Whitlow M

更新日期：2002-12-31 00:00:00

abstract：:Comparative studies on the salt titration and the related kinetics for poly(dG-dC) X poly(dG-dC) in pH 7.0 and 3.8 solutions clearly suggest that base protonation facilitates the kinetics of B-Z interconversion although the midpoint for such a transition in acidic solution (2.0-2.1 M NaCl) is only slightly lower than ...

journal_title：Biochemistry

authors： Chen FM

更新日期：1984-12-04 00:00:00

abstract：:Although tryptophan synthase catalyzes a number of pyridoxal phosphate dependent beta-elimination and beta-replacement reactions that are also catalyzed by tryptophanase, a principal and puzzling difference between the two enzymes lies in the apparent inability of tryptophan synthase to catalyze beta-elimination of in...

journal_title：Biochemistry

authors： Ahmed SA,Martin B,Miles EW

更新日期：1986-07-29 00:00:00

abstract：:Proteins from thermophiles possess high thermostability. The stabilization mechanisms differ between archaeal and bacterial proteins, whereby archaeal proteins are mainly stabilized via hydrophobic interactions and bacterial proteins by ion pairs. High stability is an important factor in promoting protein evolution, b...

journal_title：Biochemistry

authors： Takano K,Aoi A,Koga Y,Kanaya S

更新日期：2013-07-16 00:00:00

abstract：:Formylglycinamide ribonucleotide amidotransferase (FGAR-AT) catalyzes the ATP- and glutamine-dependent formation of formylglycinamidine ribonucleotide, ADP, P(i), and glutamate in the fourth step of de novo purine biosynthesis. Like all amidotransferases (ATs), FGAR-AT is proposed to channel ammonia between a glutamin...

journal_title：Biochemistry

authors： Hoskins AA,Anand R,Ealick SE,Stubbe J

更新日期：2004-08-17 00:00:00

abstract：:The activity of serine carboxypeptidases is dependent on a catalytic triad, an oxyanion hole, and a binding site equivalent to those found in the serine endopeptidases. The action of carboxypeptidase Y on substrates containing amino acids, alcohols, and amines as leaving groups is described. It is demonstrated that th...

journal_title：Biochemistry

authors： Stennicke HR,Mortensen UH,Breddam K

更新日期：1996-06-04 00:00:00

abstract：:We have studied the Cu(II), Co(II), and Fe(III) complexes of the antineoplastic drug bleomycin by using electron spin--echo envelope spectroscopy. For all three complexes, nitrogen coordination of the metal ions is demonstrated. For the Cu(II)-- and Co(II)--drug complexes, we have been able to identify imidazole as a ...

journal_title：Biochemistry

authors： Burger RM,Adler AD,Horwitz SB,Mims WB,Peisach J

更新日期：1981-03-17 00:00:00

abstract：:Helix-junction-helix (HJH) motifs are flexible building blocks of RNA architecture that help define the orientation and dynamics of helical domains. They are also frequently involved in adaptive recognition of proteins and small molecules and in the formation of tertiary contacts. Here, we use a battery of nuclear mag...

journal_title：Biochemistry

authors： Merriman DK,Xue Y,Yang S,Kimsey IJ,Shakya A,Clay M,Al-Hashimi HM

更新日期：2016-08-16 00:00:00