Abstract:
:The recruitment and organization of clathrin at endocytic sites first to form coated pits and then clathrin-coated vesicles depend on interactions between the clathrin N-terminal domain (TD) and multiple clathrin binding sequences on the cargo adaptor and accessory proteins that are concentrated at such sites. Up to four distinct protein binding sites have been proposed to be present on the clathrin TD, with each site proposed to interact with a distinct clathrin binding motif. However, an understanding of how such interactions contribute to clathrin coat assembly must take into account observations that any three of these four sites on clathrin TD can be mutationally ablated without causing loss of clathrin-mediated endocytosis. To take an unbiased approach to mapping binding sites for clathrin-box motifs on clathrin TD, we used isothermal titration calorimetry (ITC) and nuclear magnetic resonance spectroscopy. Our ITC experiments revealed that a canonical clathrin-box motif peptide from the AP-2 adaptor binds to clathrin TD with a stoichiometry of 3:1. Assignment of 90% of the total visible amide resonances in the TROSY-HSQC spectrum of (13)C-, (2)H-, and (15)N-labeled TD40 allowed us to map these three binding sites by analyzing the chemical shift changes as clathrin-box motif peptides were titrated into clathrin TD. We found that three different clathrin-box motif peptides can each simultaneously bind not only to the previously characterized clathrin-box site but also to the W-box site and the β-arrestin splice loop site on a single TD. The promiscuity of these binding sites can help explain why their mutation does not lead to larger effects on clathrin function and suggests a mechanism by which clathrin may be transferred between different proteins during the course of an endocytic event.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Zhuo Y,Cano KE,Wang L,Ilangovan U,Hinck AP,Sousa R,Lafer EMdoi
10.1021/acs.biochem.5b00065subject
Has Abstractpub_date
2015-04-28 00:00:00pages
2571-80issue
16eissn
0006-2960issn
1520-4995journal_volume
54pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::VanX, one of the five proteins required for the vancomycin-resistant phenotype in clinically pathogenic Enterococci, is a zinc-containing d-Ala-d-Ala dipeptidase. To identify potential zinc ligands and begin defining the active site residues, we have mutated the 2 cysteine, 5 histidine, and 4 of the 28 aspartate and g...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970543u
更新日期:1997-08-26 00:00:00
abstract::Calcium trapped within sonicated and resealed bovine rod outer segment disks is released upon light exposure with a stoichiometry of 0.75 +/- 0.05 calcium for each rhodopsin bleached. The amount of calcium liberated is proportional to the amount of bleaching in the range of 20 to 100% bleaching and is relatively insen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00626a025
更新日期:1977-04-05 00:00:00
abstract::Human hemoglobin produced in the Escherichia coli coexpression system of Hernan et al. [(1992) Biochemistry 31, 8619-8628] has been transformed into a functionally homogeneous protein whose properties closely approximate those of normal hemoglobin A. Both of the alpha and beta chains of this hemoglobin contain a valin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981986g
更新日期:1999-01-19 00:00:00
abstract::Extensive studies of the physiological protein-protein electron-transfer (ET) complex between yeast cytochrome c peroxidase (CcP) and cytochrome c (Cc) have left unresolved questions about how formation and dissociation of binary and ternary complexes influence ET. We probe this issue through a study of the photocycle...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500888y
更新日期:2015-02-10 00:00:00
abstract::A PS I membrane preparation from a PS II deficient mutant of Synechocystis sp. PCC 6803 (psb DI/DII/C) was investigated by picosecond photovoltage and fluorescence measurements. The photovoltage kinetics show two distinct phases. At low excitation energies the fast phase correlates with the fluorescence decay time con...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00195a001
更新日期:1994-07-26 00:00:00
abstract::Fusion of a prokaryotic promoter to a yeast tRNA gene provides a means for uncoupling analyses of mutations affecting splicing from requirements for transcription and other processing steps. For this purpose, a phage lambda promoter was fused to the Saccharomyces cerevisiae tRNATyr(SUP3a) coding sequence. This fusion ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00240a017
更新日期:1991-07-02 00:00:00
abstract::Thyroxine and analogues inhibit rat liver aminoacyl-tRNA synthetase activity for phenylalanine and tyrosine. A high yield purification of the major cytoplasmic form of phenylalanyl-tRNA synthetase (C1) and its characterization is reported. Polyribosome-bound and other sedimentable forms are found to be indistinguishab...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00647a017
更新日期:1976-01-27 00:00:00
abstract::The complete amino acid sequence of ragweed pollen allergen Ra3 has been determined. The molecule consists of 101 amino acid residues and to date is the only allergen isolated from Ambrosia elatior (short ragweed) which contains carbohydrate. This particular preparation of allergen has a single, unique amino acid sequ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00566a010
更新日期:1980-12-09 00:00:00
abstract::In Campylobacter jejuni 2,4-diacetamido-2,4,6-trideoxy-alpha-d-glucopyranose, termed N,N'-diacetylbacillosamine (Bac2,4diNAc), is the first carbohydrate in the glycoprotein N-linked heptasaccharide. With uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) as a starting point, two enzymes of the general protein glycos...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061456h
更新日期:2006-11-14 00:00:00
abstract::Supported phospholipid bilayers prepared by Langmuir-Blodgett techniques were introduced recently as a new model membrane system [Tamm, L.K., & McConnell, H.M. (1985) Biophys. J. 47, 105-113]. Here, supported bilayers are applied to study the lateral diffusion and lateral distribution of membrane-bound monoclonal anti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00405a009
更新日期:1988-03-08 00:00:00
abstract::CTP synthetase [EC 6.3.4.2, UTP:ammonia ligase (ADP-forming)] from the yeast Saccharomyces cerevisiae catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to the C-4 position of UTP to form CTP. In this work, we demonstrated that CTP synthetase utilized dUTP as a substrate to synthesize dCTP. The ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9920127
更新日期:1999-12-14 00:00:00
abstract::The slowly hydrolyzed ATP analog adenosine 5'-(gamma-thiotriphosphate) (ATP gamma S) has been used in many studies of the muscle motor protein myosin in order to form a stable "weak binding" state analogous to the actin-S1-ATP complex However, the results from studies using ATP gamma S do not always agree with the res...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00049a018
更新日期:1995-12-12 00:00:00
abstract::A study of the regular secondary structure elements of recombinant human interleukin-1 beta has been carried out using NMR spectroscopy. Using a randomly 15N labeled sample, a number of heteronuclear three- and two-dimensional NMR experiments have been performed, which have enabled a complete analysis of short-, mediu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00471a023
更新日期:1990-05-15 00:00:00
abstract::Tularemia is a severe infectious disease in humans caused by the Gram-negative bacterium Francisella tularensis (Ft). Because of its low infectious dose, high mortality rate, and the threat of its large-scale dissemination in weaponized form, development of vaccines and immunotherapeutics against Ft is essential. Ft l...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201450v
更新日期:2011-12-20 00:00:00
abstract::Filamins are a family of actin cross-linking proteins that are primarily localized in the cortical cytoplasm of all mammalian cells. Until now, three major isoforms (filamins a, b, and c) have been identified, that were shown to be differentially expressed and/or localized in different tissues. An amino-terminal doubl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026501+
更新日期:2003-01-21 00:00:00
abstract::To study the effects of methylation damage on DNA replication in vitro, the plasmid pSVori containing the SV40 origin of replication was reacted with N-methyl-N-nitrosourea and used as a substrate for SV40 T antigen dependent replication by HeLa cell extracts. The plasmid was methylated with a range of N-methyl-N-nitr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00212a035
更新日期:1993-12-14 00:00:00
abstract::We introduced a stop codon in place of the ATT codon encoding Ile283 (numbered from the Met initiation codon) in the petA gene from Chlamydomonas reinhardtii. The resulting protein was expected to be truncated on its carboxy-terminus end, lacking the last 35 amino acids. This region of the polypeptide sequence encompa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00022a021
更新日期:1995-06-06 00:00:00
abstract::Spectroscopic methods are used to probe the interactions of several anilinoacridine analogues with calf thymus DNA over a wide range of temperatures and sodium chloride concentrations. The structurally similar compounds m-AMSA, AMSA (both active as antitumor agents), and o-AMSA (inactive as an antitumor agent) have be...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00231a025
更新日期:1991-04-30 00:00:00
abstract::We have investigated some properties related to interaction with DNA and recognition of AT-rich sequences of netropsin-oxazolopyridocarbazole (Net-OPC) (Mrani et al., 1990), which is a hybrid groove-binder-intercalator. The hybrid molecule Net-OPC binds to poly[d(A-T)] at two different sites with Kapp values close to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00220a029
更新日期:1991-02-12 00:00:00
abstract::Kinetic models are presented that allow the Na(+),K(+)-ATPase steady-state turnover number to be estimated at given intra- and extracellular concentrations of Na(+), K(+), and ATP. Based on experimental transient kinetic data, the models utilize either three or four steps of the Albers-Post scheme, that is, E(2) --> E...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0355443
更新日期:2004-03-02 00:00:00
abstract::The membrane-bound quinoprotein glucose dehydrogenase (mGDH) in Escherichia coli contains pyrroloquinoline quinone (PQQ) and participates in the direct oxidation of D-glucose to D-gluconate by transferring electrons to ubiquinone (UQ). To elucidate the mechanism of ubiquinone reduction by mGDH, we applied a pulse radi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051347n
更新日期:2005-10-18 00:00:00
abstract::The left-handed polyproline II (P(II)) helix is a structure that has been given a great deal of attention lately because of its role in a wide variety of physiologically important processes and potential significance in protein unfolded states. Recent work by several authors has shown that residues besides proline can...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049922v
更新日期:2004-05-18 00:00:00
abstract::In bacteria, the transcription of virulence genes is usually controlled by a cell density-dependent process known as "quorum sensing" (QS). QS relies on small diffusible signaling molecules that cross the bacterial cell wall and activate target transcription factors after a threshold concentration has been reached. Be...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900123j
更新日期:2009-11-03 00:00:00
abstract::The structure formed from the DNA oligonucleotide d(G4T4G4) (Oxy-1.5), which contains the Oxytricha telomere repeat T4G4, has been investigated by two-dimensional 1H and 31P NMR spectroscopy. Sequence-specific assignments have been obtained for the 1H and 31P resonances, using a combination of methods including compar...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00084a040
更新日期:1993-08-24 00:00:00
abstract::Toxic shock syndrome toxin-1 (TSST-1), the potent staphylococcal exoprotein linked to most cases of the toxic shock syndrome, is a V beta-restricted T-cell mitogen (a so-called "superantigen"). TSST-ovine (TSST-O) is a natural variant of TSST-1, and is produced by certain ovine mastitis-associated strains of Staphyloc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00209a016
更新日期:1994-11-01 00:00:00
abstract::The explosion of protein sequence information requires that current strategies for function assignment evolve to complement experimental approaches with computationally based function prediction. This necessitates the development of strategies based on the identification of sequence markers in the form of specificity ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201181q
更新日期:2011-10-18 00:00:00
abstract::Competitive inhibition of the monophenolase reaction of tyrosinase by a variety of compounds was investigated with respect to temperature and pH. Derivatives of benzoic acid as well as p-nitrophenol were the best inhibitors. Toluene and several N-heterocycles were all weak inhibitors. Thermodynamic parameters of tolue...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00185a010
更新日期:1994-05-17 00:00:00
abstract::Manganese superoxide dismutase (MnSOD) cycles between the Mn(II) and Mn(III) states during the catalyzed disproportionation of O(2)(*-), a catalysis that is limited at micromolar levels of superoxide by a peroxide-inhibited complex with the metal. We have investigated the role in catalysis and inhibition of the conser...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011047f
更新日期:2001-10-09 00:00:00
abstract::Electrogenic events associated with the activity of the melibiose permease (MelB), a transporter from Escherichia coli, were investigated. Proteoliposomes containing purified MelB were adsorbed to a solid supported lipid membrane, activated by a substrate concentration jump, and transient currents were measured. When ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0489053
更新日期:2004-10-05 00:00:00
abstract::We previously showed that beta-amyloid precursor protein (APP) is cleaved not only in the middle of the membrane (gamma-cleavage) but also at novel cleavage sites close to the membrane/cytoplasmic boundary (epsilon-cleavage), releasing APP intracellular domains (AICDs) 49-99 and 50-99. To learn more about the relation...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049399k
更新日期:2004-10-26 00:00:00