Abstract：
:Filamins are a family of actin cross-linking proteins that are primarily localized in the cortical cytoplasm of all mammalian cells. Until now, three major isoforms (filamins a, b, and c) have been identified, that were shown to be differentially expressed and/or localized in different tissues. An amino-terminal double CH-domain actin binding domain, and a dimerization region in the carboxy-terminal portion of the protein are the molecular basis for its actin cross-linking activity. Chemical cross-linking of bacterially expressed recombinant proteins was used to demonstrate that in all three filamin isoforms the most carboxy-terminally situated immunoglobulinlike domain is required and sufficient for dimerization. The efficiency of the dimerization was increased upon inclusion of the preceding hinge 2 region, indicating a function for this region in the regulation of dimerization. By mixing recombinant proteins derived from different filamin isoforms, we found that heterodimer formation is possible between filamins b and c but not between filamin a and the other two filamins. This selectivity of dimerization might provide a further molecular explanation for the differential intracellular sorting of filamin isoforms and their distinct properties.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Himmel M,Van Der Ven PF,Stöcklein W,Fürst DOdoi
10.1021/bi026501+subject
Has Abstractpub_date
2003-01-21 00:00:00pages
430-9issue
2eissn
0006-2960issn
1520-4995journal_volume
42pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:By employing a general biosynthetic method for the elaboration of proteins containing unnatural amino acid analogues, we incorporated (aminooxy)acetic acid into positions 10 and 27 of Escherichia coli dihydrofolate reductase. Introduction of the modified amino acid into DHFR was accomplished in an in vitro protein bio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi020352d
更新日期：2002-09-24 00:00:00
abstract：:N,N'-dicyclohexylcarbodiimide (DCCD) has been reported to inhibit proton translocation by cytochrome bc(1) and b(6)f complexes without significantly altering the rate of electron transport, a process referred to as decoupling. To understand the possible role of DCCD in inhibiting the protonogenic reactions of cytochro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001482u
更新日期：2000-12-26 00:00:00
abstract：:The mature form of subtilisin is an unusual example of a monomeric protein with a high kinetic barrier to folding and unfolding. Using site-directed mutagenesis of subtilisin BPN', we are attempting to determine the physical and energetic nature of the kinetic barrier. The high-affinity calcium-binding site A has been...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00090a012
更新日期：1993-10-05 00:00:00
abstract：:Type I protein arginine methyltransferases (PRMTs) catalyze asymmetric dimethylation of various proteins, and their dysregulations often correlate with tumorigenesis or developmental deficiency. Recent studies have focused on the in vivo substrate identification and the enzyme mechanism with peptide substrates. Howeve...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00995
更新日期：2015-12-29 00:00:00
abstract：:In order to investigate the changes of protonation or environment of carboxylic residues occurring upon photoreduction of the secondary quinone acceptor (QB) in the reaction center (RC) of the photosynthetic bacteria Rhodobacter sphaeroides 2.4.1., we have performed light-induced Fourier transform infrared (FTIR) spec...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00045a013
更新日期：1995-11-14 00:00:00
abstract：:The newly discovered oncogenic protein gankyrin, which contains six ankyrin repeats, has been reported to be involved in the phosphorylation and degradation of the retinoblastoma gene product, Rb. Using in vitro systems, we have identified a peptide fragment of gankyrin, 176LHLACDEERN185, which is responsible for bind...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011550s
更新日期：2002-03-26 00:00:00
abstract：:Fourier transform infrared (FTIR) spectroscopy and a library of FTIR marker bands have been used to examine the structure and relative flexibilities conferred by different flanking sequences on the EcoRI binding site. This approach allowed us to examine unique peaks and subtle changes in the spectra of d(AAAGAATTCTTT)...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8015235
更新日期：2009-02-17 00:00:00
abstract：:The beta(2) subunit of a class Ia or Ib ribonucleotide reductase (RNR) is activated when its carboxylate-bridged Fe(2)(II/II) cluster reacts with O(2) to oxidize a nearby tyrosine (Y) residue to a stable radical (Y(*)). During turnover, the Y(*) in beta(2) is thought to reversibly oxidize a cysteine (C) in the alpha(2...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi8017625
更新日期：2008-12-30 00:00:00
abstract：:The plasma membrane serotonin transporter (SERT) has an important role in terminating serotonergic neurotransmission by re-uptake of 5-HT from the synaptic cleft. The expression of SERT on the cell surface is therefore a critical factor. In this study, we examined the role of the carboxyl terminus of SERT in trafficki...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0508688
更新日期：2006-01-31 00:00:00
abstract：:The kinetic parameters (Km and Vmax) of sugar-modified analogues of inosine and guanosine have been determined with human erythrocytic purine nucleoside phosphorylase (PNP). Steric alterations at the 2' and 3' positions greatly lessened or abolished substrate activity. However, the 5'-deoxy- and 2',5'-dideoxy-beta-D-r...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00542a016
更新日期：1980-01-08 00:00:00
abstract：:Proteogenomic identification of translated small open reading frames in humans has revealed thousands of microproteins, or polypeptides of fewer than 100 amino acids, that were previously invisible to geneticists. Hundreds of microproteins have been shown to be essential for cell growth and proliferation, and many reg...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00672
更新日期：2020-10-27 00:00:00
abstract：:The production of proteins via recombinant DNA technology often requires the in vitro folding of inclusion bodies, which are protein aggregates. To create a more efficient redox buffer for the in vitro folding of disulfide containing proteins, aromatic thiols were investigated for their ability to increase the folding...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034305c
更新日期：2003-10-14 00:00:00
abstract：:D(--)-beta-Hydroxybutyrate dehydrogenase of beef heart mitochondria is inhibited by modifiers of disulfides, thiols, and vicinal dithiols [Phelps, D. C., & Hatefi, Y. (1981) Biochemistry 20 (preceding paper in this issue)]. The vicinal dithiol can be reversibly oxidized by diamide, resulting in activity inhibition, an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00506a002
更新日期：1981-02-03 00:00:00
abstract：:We added phospholipase C-delta 1 (PLC-delta) to the aqueous subphase beneath monolayers formed from mixtures of phosphatidylinositol 4,5-bisphosphate (2% PIP2), phosphatidylserine (33% PS), and phosphatidylcholine (65% PC) and then measured the initial rate of hydrolysis of PIP2 after addition of 10 microM free calciu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00166a006
更新日期：1992-12-29 00:00:00
abstract：:The binding of Ca2+ and the resulting change in catalytic specificity that allows phosphorylation of the calcium ATPase of sarcoplasmic reticulum by ATP were examined by measuring the amount of phosphoenzyme formation from [32P]ATP, or 45Ca incorporation into vesicles, after the simultaneous addition of ATP and EGTA a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00423a018
更新日期：1988-11-15 00:00:00
abstract：:The dynamics of the backbone and (some of) the side chains of lac headpiece (1-56; lac HP56) have been studied for the free protein and for its complex with lac half-operator DNA by 15N T1 and T1p relaxation measurements combined with [1H-15N] NOE experiments. For the structurally well-defined part of the free lac HP5...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961670d
更新日期：1997-01-07 00:00:00
abstract：:RalA and RalB are members of the Ras family of small G proteins and are activated downstream of Ras via RalGEFs. The RalGEF-Ral axis represents one of the major effector pathways controlled by Ras and as such is an important pharmacological target. RalA and RalB are approximately 80% identical at the amino acid level;...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501530u
更新日期：2015-02-17 00:00:00
abstract：:G-Actin is a globular protein (Mr 42 300) known to have three cysteine residues that are at least partially exposed and chemically reactive (Cys-10, -284, and -374). When G-actin was reacted with 3-bromo-1,1,1-trifluoropropanone, three resolvable 19F resonances were observed in the 19F NMR spectrum. This fluorinated G...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00356a050
更新日期：1986-04-22 00:00:00
abstract：:Aminopeptidases catalyze the hydrolysis of amino acid residues from the amino terminus of peptide substrates. They are found in most cells and tissues, and their activity has been implicated in myriad fundamental biochemical and physiological processes. Nevertheless, little is known about the structure of the aminopep...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00131a034
更新日期：1992-04-28 00:00:00
abstract：:Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) is a thermostable archaeal enzyme and a member of the error-prone and lesion-bypass Y-family. In this paper, for the first time, the fidelity of a Y-family polymerase, Dpo4, was determined using pre-steady-state kinetic analysis of the incorporation of a single nucle...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0357457
更新日期：2004-02-24 00:00:00
abstract：:Oligodeoxynucleotides modified site-specifically with dG-(+)-trans- and dG-(+)-cis-anti-BPDE (7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene) or dG-(-)-trans- and dG-(-)-cis-anti-BPDE were used as templates in primer extension reactions catalyzed by the Klenow fragment of Escherichia coli DNA polymerase I....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00080a027
更新日期：1993-07-27 00:00:00
abstract：:A fluorescence quenching method was developed for determining partition coefficients and diffusional rates of small molecules in cell membranes. This method involves quenching the fluorescence of carbazole-labeled membranes by hydrophobic molecules that partition into membranes. Cell membrane phospholipids of mouse LM...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00316a021
更新日期：1984-10-09 00:00:00
abstract：:A method is presented for the rapid determination of substrate selection in a manner that is not restricted to conditions of metabolic and isotopic steady state. Competition between several substrates can be assessed directly and continuously in a single experiment, allowing the effect of interventions to be studied. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00481a002
更新日期：1990-07-24 00:00:00
abstract：:We have prepared the following cadmium-113-substituted derivatives of bovine superoxide dismutase and recorded the nuclear magnetic resonance (NMR) spectrum of the cadmium: 2Cd(II), in which Cd(II) is presumed to bind to the Zn(II) site and the copper site is unoccupied, and 2Cd(II)--2Cu(I), which is analogous to the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00544a031
更新日期：1980-02-05 00:00:00
abstract：:To clarify the function of the hydrophilic carboxyl-terminal tail of human erythrocyte membrane band 3 protein (HEM-B3), we purified two peptides, C1 (Ala893-Val911) and KS4 (Gly647-Arg656), from human erythrocyte band 3 protein preparations. Purified C1 peptides at concentrations from 5 to 80 microM were incubated wi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035281c
更新日期：2004-02-17 00:00:00
abstract：:Group VIA phospholipase A(2) (iPLA(2)beta) is expressed in phagocytes, vascular cells, pancreatic islet beta-cells, neurons, and other cells and plays roles in transcriptional regulation, cell proliferation, apoptosis, secretion, and other events. A bromoenol lactone (BEL) suicide substrate used to study iPLA(2)beta f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060502a
更新日期：2006-05-23 00:00:00
abstract：:Sulfenic acid is formed upon oxidation of thiols and is a central intermediate in the redox modulation of an increasing number of proteins. Methods for quantifying or even detecting sulfenic acid are scarce. Herein, the reagent 7-chloro-4-nitrobenz-2-oxa-1,3-diazole was determined not to be suitable as a chromophoric ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701520y
更新日期：2008-01-08 00:00:00
abstract：:A new strategy to form stable and sequence-specific triple helical DNA structures at mixed purine/pyrimidine sequences using a combination of four C-glycosides (TRIPsides) has been described [Li et al. (2003) J. Am. Chem. Soc. 125, 2084]. The partial realization of the approach is demonstrated by incorporating two of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035648d
更新日期：2004-02-17 00:00:00
abstract：:The chemical and enzymatic syntheses of bacteriophage lambda pseudo operator DNA are described. The 17 base-paired duplex contains the DNA which has been proposed as the binding site for cI repressor protein. The synthetic duplex is twofold symmetric and represents the best possible nucleotide summation of the six pro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00638a013
更新日期：1977-09-20 00:00:00
abstract：:The low level of enzymatic activity of certain alpha 2-macroglobulin-proteinase complexes could be important to the function of factor VIII/von Willebrand glycoprotein since it is especially sensitive to proteolytic cleavage. To test this possibility, complexes of alpha 2-macroglobulin with plasmin, trypsin, and throm...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00275a018
更新日期：1983-03-15 00:00:00
