Human erythrocytic purine nucleoside phosphorylase: reaction with sugar-modified nucleoside substrates.

Abstract:

:The kinetic parameters (Km and Vmax) of sugar-modified analogues of inosine and guanosine have been determined with human erythrocytic purine nucleoside phosphorylase (PNP). Steric alterations at the 2' and 3' positions greatly lessened or abolished substrate activity. However, the 5'-deoxy- and 2',5'-dideoxy-beta-D-ribofuranosyl and the alpha-L-lyxosyl analogues were good substrates, indicating that the 5'-hydroxyl and the orientation of the 5'-hydroxy-methyl group are not important for binding. The sugar phosphate analogue, 5-deoxyribose 1-phosphate, was synthesized from 5'-deoxyinosine with immobilized PNP, and its presence was verified by using it in the enzymic synthesis of 5'-deoxyguanosine. The adenosine versions of the 5'-modified analogues were also found to react with adenosine deaminase, albeit at less than 1% of Vmax.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Stoeckler JD,Cambor C,Parks RE Jr

doi

10.1021/bi00542a016

subject

Has Abstract

pub_date

1980-01-08 00:00:00

pages

102-7

issue

1

eissn

0006-2960

issn

1520-4995

journal_volume

19

pub_type

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