Abstract：
:A new strategy to form stable and sequence-specific triple helical DNA structures at mixed purine/pyrimidine sequences using a combination of four C-glycosides (TRIPsides) has been described [Li et al. (2003) J. Am. Chem. Soc. 125, 2084]. The partial realization of the approach is demonstrated by incorporating two of the four TRIPsides into oligomers that can potentially fold into intramolecular triplexes that contain one or two major groove crossovers of the purine Hoogsteen H-bond information. Using temperature-dependent electronic and fluorescence spectroscopy and differential scanning calorimetry, it is demonstrated that stable triplexes form at physiological conditions at non-homopurine targets. In addition, triplexes using the TRIPsides form in a highly sequence specific manner.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Li JS,Shikiya R,Marky LA,Gold Bdoi
10.1021/bi035648dsubject
Has Abstractpub_date
2004-02-17 00:00:00pages
1440-8issue
6eissn
0006-2960issn
1520-4995journal_volume
43pub_type
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