Membrane topology of the di- and tripeptide transport protein of Lactococcus lactis.

abstract：:The respiratory chain complexes can arrange into multienzyme assemblies, so-called supercomplexes. We present the first 3D map of a respiratory chain supercomplex. It was determined by random conical tilt electron microscopy analysis of a bovine supercomplex consisting of complex I, dimeric complex III, and complex IV...

journal_title：Biochemistry

authors： Schäfer E,Dencher NA,Vonck J,Parcej DN

更新日期：2007-11-06 00:00:00

abstract：:Reaction center (RC) complexes isolated from a Zn-bacteriochlorophyll (BChl) a-containing purple bacterium, Acidiphilium rubrum, were characterized by absorption, circular dichroism, and magnetic circular dichroism (MCD) spectroscopy. The oxidized-minus-reduced difference spectra indicated that, in this RC, the Zn-BCh...

journal_title：Biochemistry

authors： Mimuro M,Kobayashi M,Shimada K,Uezono K,Nozawa T

更新日期：2000-04-11 00:00:00

abstract：:In Gram-negative bacteria, the first step of lipid A biosynthesis is catalyzed by UDP-N-acetylglucosamine acyltransferase (LpxA) through the transfer of a R-3-hydroxyacyl chain from the acyl carrier protein (ACP) to the 3-hydroxyl group of UDP-GlcNAc. Previous studies suggest that LpxA is a critical determinant of the...

journal_title：Biochemistry

authors： Smith EW,Zhang X,Behzadi C,Andrews LD,Cohen F,Chen Y

更新日期：2015-09-29 00:00:00

abstract：:Telomerase is a ribonucleoprotein that participates in the maintenance of telomere length. Its activity is up-regulated in many tumor types, suggesting that it may be a novel target for chemotherapy. The RNA component of telomerase contains an active site that plays at least two roles&sbd;binding telomere ends and tem...

journal_title：Biochemistry

authors： Hamilton SE,Pitts AE,Katipally RR,Jia X,Rutter JP,Davies BA,Shay JW,Wright WE,Corey DR

更新日期：1997-09-30 00:00:00

abstract：:The amino acid composition and sequence of EIIMtl is known [Lee, C. A., & Saier, M. H., Jr. (1983) J. Biol. Chem. 258, 10761-10767]. This information was combined, in the present study, with quantitative amino acid analysis to determine the molar concentration of the enzyme. The stoichiometry of phosphoryl group incor...

journal_title：Biochemistry

authors： Pas HH,ten Hoeve-Duurkens RH,Robillard GT

更新日期：1988-07-26 00:00:00

abstract：:Chicken liver phosphoenolpyruvate carboxykinase (PEPCK) is activated by Cr2+ as the sole activator under anaerobic conditions. PEPCK was modified with Cr3+, starting with either Cr2+ or Cr3+. Cr3+ has the distinct advantage of being a paramagnetic cation that could serve as a paramagnetic probe. Activators Mn2+, Mg2+,...

journal_title：Biochemistry

authors： Hlavaty JJ,Nowak T

更新日期：1998-06-02 00:00:00

abstract：:Out of over 60 single-Cys mutants in putative periplasmic loops in lactose permease, three mutants [Tyr101 --> Cys (loop III/IV), Leu313 --> Cys (loop IX/X), and Ser375 --> Cys (loop XI/XII)] spontaneously form disulfide-linked dimers, indicating that these loops are located on the periphery of the 12-helix bundle tha...

journal_title：Biochemistry

authors： Sun J,Kaback HR

更新日期：1997-09-30 00:00:00

abstract：:Release of neurotransmitter from synaptic vesicles requires the Ca2+/phospholipid-binding protein synaptotagmin 1. There is considerable evidence that cooperation between the tandem C2 domains of synaptotagmin is a requirement of regulated exocytosis; however, high-resolution structural evidence for this interaction h...

journal_title：Biochemistry

authors： Fuson KL,Montes M,Robert JJ,Sutton RB

更新日期：2007-11-13 00:00:00

abstract：:A novel and efficient enzyme kinetics assay using electrospray ionization mass spectrometry was developed and applied to the bacterial carbohydrate sulfotransferase (NodST). NodST catalyzes the sulfuryl group transfer from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to chitobiose, generating 3'-phosphoadenosine 5'-ph...

journal_title：Biochemistry

authors： Pi N,Armstrong JI,Bertozzi CR,Leary JA

更新日期：2002-11-05 00:00:00

abstract：:Human angiotensin-converting enzyme (ACE) has two homologous domains, the N and C domains, with differing substrate preferences. X-ray crystal structures of the C and N domains complexed with various inhibitors have allowed identification of active site residues that might be important for the molecular basis of this ...

journal_title：Biochemistry

authors： Watermeyer JM,Kröger WL,O'Neill HG,Sewell BT,Sturrock ED

更新日期：2008-06-03 00:00:00

abstract：:The intestinal fatty acid binding protein (IFABP) is a small (15 kDa) protein consisting mostly of 10 antiparallel beta-strands (A-J) and a small helical region that serves as a portal for the ligand. Two beta-sheet structures (strands A-E and F-J) surround a cavity into which the ligand binds. In this work, we invest...

journal_title：Biochemistry

authors： Rajabzadeh M,Kao J,Frieden C

更新日期：2003-10-28 00:00:00

abstract：:1H NMR studies on the nonselective endothelin receptor agonist sarafotoxin SRTb have identified a helix between residues Asp 8 and His 16, and a beta-turn involving residues Cys 3 to Met 6; however, the biologically important C-terminal five residues were found to be conformationally variable. The average RMSD, measur...

journal_title：Biochemistry

authors： Atkins AR,Martin RC,Smith R

更新日期：1995-02-14 00:00:00

abstract：:Factor V and factor Va binding to single bilayer phospholipid vesicles was investigated by light-scattering intensity measurements. This technique allows the measurement of free and phospholipid-bound protein concentrations from which equilibrium constants can be obtained. As controls, the Ca2+-dependent phospholipid ...

journal_title：Biochemistry

authors： Bloom JW,Nesheim ME,Mann KG

更新日期：1979-10-02 00:00:00

abstract：:Tau is a microtubule associated protein implicated in the pathogenesis of several neurodegenerative diseases. Because of the channel forming properties of other amyloid peptides, we employed planar lipid bilayers and atomic force microscopy to test tau for its ability to form ion permeable channels. Our results demons...

journal_title：Biochemistry

authors： Patel N,Ramachandran S,Azimov R,Kagan BL,Lal R

更新日期：2015-12-22 00:00:00

abstract：:Various agents are able to stimulate the EGF receptor protein tyrosine kinase in the absence of ligand binding. To characterize their mechanism of action, we investigated their effects on the kinase activity of the intracellular domain of the EGF receptor (EGFR-IC). EGFR-IC (67 kDa) lacking the extracellular domain an...

journal_title：Biochemistry

authors： Mohammadi M,Honegger A,Sorokin A,Ullrich A,Schlessinger J,Hurwitz DR

更新日期：1993-08-31 00:00:00

abstract：:Thermodynamic parameters of helix formation were measured spectroscopically for 16 oligoribonucleotides containing either internal GU mismatches or the corresponding AU pairs. Internal GU mismatches stabilize each helix, but not as much as the corresponding AU pairs. The differences in the enthalpy and entropy changes...

journal_title：Biochemistry

authors： Sugimoto N,Kierzek R,Freier SM,Turner DH

更新日期：1986-09-23 00:00:00

abstract：:The ligand-binding dynamics and the reaction with O(2) of the fully (five-electron) reduced cytochrome caa(3) from the thermohalophilic bacterium Rhodothermus (R.) marinus were investigated. The enzyme is a proton pump which has all the residues of the proton-transfer pathways found in the mitochondrial-like enzymes c...

journal_title：Biochemistry

authors： Sigurdson H,Namslauer A,Pereira MM,Teixeira M,Brzezinski P

更新日期：2001-09-04 00:00:00

abstract：:The successive high-resolution differential scanning calorimetric (DSC) thermograms for aqueous dispersions of a homologous series of mixed-chain phosphatidylcholines, C(X):C(X + 6)PC, have been recorded and analyzed. In this series of saturated mixed-chain phosphatidylcholines, the total number of carbon atoms in the...

journal_title：Biochemistry

authors： Wang ZQ,Lin HN,Huang CH

更新日期：1990-07-31 00:00:00

abstract：:The bacterial chemoreceptor complex governs signal detection and the upstream elements of chemotactic behavior, but the detailed molecular mechanism is still unclear. We have assembled nativelike functional arrays of an aspartate receptor cytoplasmic fragment (CF) with its two cytoplasmic protein partners (CheA and Ch...

journal_title：Biochemistry

authors： Harris MJ,Struppe JO,Wylie BJ,McDermott AE,Thompson LK

更新日期：2016-07-05 00:00:00

abstract：:Ricin A-chain (RTA) catalyzes the hydrolytic depurination of a specific adenosine at position 4324 of 28S rRNA. Kinetic isotope effects on the hydrolysis of a small 10mer stem-tetraloop oligonucleotide substrate established the mechanism of the reaction as D(N)*A(N), involving an oxacarbenium ion intermediate in a hig...

journal_title：Biochemistry

authors： Roday S,Amukele T,Evans GB,Tyler PC,Furneaux RH,Schramm VL

更新日期：2004-05-04 00:00:00

abstract：:By superimposing data obtained by photo-cross-linking RGD-containing ligands to the human alpha(V)beta(3) integrin onto the crystal structure of the ectopic domain of this receptor (Xiong et al. (2001) Science 294, 339-345), we have identified the binding site for the RGD triad within this integrin. We synthesized thr...

journal_title：Biochemistry

authors： Yahalom D,Wittelsberger A,Mierke DF,Rosenblatt M,Alexander JM,Chorev M

更新日期：2002-07-02 00:00:00

abstract：:Protein folding is a process in which an extended polypeptide chain acquires compact packing through the formation of specific secondary and tertiary structures and hydrophobic interactions. Although much attention has been paid to secondary and tertiary structures, there is no definitive view about the relationship b...

journal_title：Biochemistry

authors： Nishii I,Kataoka M,Tokunaga F,Goto Y

更新日期：1994-04-26 00:00:00

abstract：:F-STOP is a microtubule-associated protein that stabilizes microtubules in a calmodulin (CaM)-dependent manner. All members of the stable tubule only polypeptide (STOP) family have a central domain that contains nearly identical multiple repeats, and a CaM binding motif is present in multiple copies within this domain...

journal_title：Biochemistry

authors： Bouvier D,Vanhaverbeke C,Simorre JP,Arlaud GJ,Bally I,Forge V,Margolis RL,Gans P,Kleman JP

更新日期：2003-10-07 00:00:00

abstract：:Retinitis pigmentosa (RP), a neurodegenerative disorder, can arise from single point mutations in rhodopsin, leading to a cascade of protein instability, misfolding, aggregation, rod cell death, retinal degeneration, and ultimately blindness. Divalent cations, such as zinc and copper, have allosteric effects on misfol...

journal_title：Biochemistry

authors： Gleim S,Stojanovic A,Arehart E,Byington D,Hwa J

更新日期：2009-03-03 00:00:00

abstract：:Nine monoclonal antibodies were used to test calcium and EDTA effects on the molecular conformation of ventricular VLC2 within myosin. Antibody epitopes were located in six domains of VLC2 using recombinant proteins. The apparent association constants of these antibodies were measured in solution in the presence of ca...

journal_title：Biochemistry

authors： Eldin P,Cathiard AM,Anoal M,Léger J,Mornet D,Léger JJ,Cornillon B

更新日期：1994-10-25 00:00:00

abstract：:In animals, the post-translational hydroxylation of hypoxia inducible factor (HIF) is a central mechanism for regulating gene expression in an oxygen-dependent manner. The oxygenase-catalyzed trans-4-prolyl hydroxylation of HIF-alpha increases its affinity for the von Hippel Lindau protein elongin B/C (VCB) ubiquitin ...

journal_title：Biochemistry

authors： Illingworth CJ,Loenarz C,Schofield CJ,Domene C

更新日期：2010-08-17 00:00:00

abstract：:Two site-directed mutant enzymes of the class A beta-lactamase from Staphylococcus aureus PC1 were produced with the goal of blocking the site that in the native enzyme is occupied by the proposed hydrolytic water molecule. The crystal structures of these two mutant enzymes, N170Q and N170M, have been determined and r...

journal_title：Biochemistry

authors： Zawadzke LE,Chen CC,Banerjee S,Li Z,Wäsch S,Kapadia G,Moult J,Herzberg O

更新日期：1996-12-24 00:00:00

abstract：:Slow conformational changes have been proposed to be responsible for the kinetic positive cooperativity of glucokinase (GK) with glucose. Induced-fit and preexisting equilibrium kinetic models have been previously suggested. In the present study, equilibrium and pre-steady-state fluorescence spectroscopy has been used...

journal_title：Biochemistry

authors： Antoine M,Boutin JA,Ferry G

更新日期：2009-06-16 00:00:00

abstract：:The solution structure of the C-terminal fragment 255-316 of thermolysin has been determined by two-dimensional proton NMR methods. For this disulfide-free fragment there was a previous proposal according to which it would fold into a stable helical structure forming a dimer at concentrations above 0.06 mM. A complete...

journal_title：Biochemistry

authors： Rico M,Jiménez MA,González C,De Filippis V,Fontana A

更新日期：1994-12-13 00:00:00

abstract：:L-Aspartate oxidase (Laspo) catalyzes the conversion of L-Asp to iminoaspartate, the first step in the de novo biosynthesis of NAD(+). This bacterial pathway represents a potential drug target since it is absent in mammals. The Laspo R386L mutant was crystallized in the FAD-bound catalytically competent form and its t...

journal_title：Biochemistry

authors： Bossi RT,Negri A,Tedeschi G,Mattevi A

更新日期：2002-03-05 00:00:00