Abstract：
:Nine monoclonal antibodies were used to test calcium and EDTA effects on the molecular conformation of ventricular VLC2 within myosin. Antibody epitopes were located in six domains of VLC2 using recombinant proteins. The apparent association constants of these antibodies were measured in solution in the presence of calcium or EDTA. An immunofluorescence study was performed to establish whether the observed effects would occur in more integrated systems, as compared to isolated proteins in solution. Our results showed (1) a slight effect of calcium on isolated VLC2, located in the aa 29-45 domain, (2) a clear-cut effect of calcium on VLC2 within myosin, only in the aa 45-59 domain, and (3) in the presence of EDTA, antibody affinities for VLC2 within myosin similar to the affinities for isolated VLC2. These results are discussed in terms of spatial arrangements and binding mechanisms between HC and VLC2. They suggest that there are two processes for stabilizing HC/VLC2 complex formation: one binding via calcium chelation and another involving hydrophobic interactions.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Eldin P,Cathiard AM,Anoal M,Léger J,Mornet D,Léger JJ,Cornillon Bdoi
10.1021/bi00208a005subject
Has Abstractpub_date
1994-10-25 00:00:00pages
12558-64issue
42eissn
0006-2960issn
1520-4995journal_volume
33pub_type
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