Abstract：
:Melittin is a 26-residue peptide which undergoes high-affinity calcium-dependent binding by calmodulin [Barnette, M.S., Daly, R., & Weiss, B. (1983) Biochem. Pharmacol. 32, 2929; Comte, M., Maulet, Y., & Cox, J.A. (1983) Biochem. J. 209, 269; Anderson, S.R., & Malencik, D.A. (1986) Calcium Cell Funct. 6, 1]. The results in this paper show that three different types of myosin light chain--the smooth muscle regulatory light chain, the smooth muscle essential light chain, and the skeletal muscle regulatory 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) light chain--also associate with melittin. The resulting complexes have dissociation constants ranging from 1.1 to 2.5 microM in the presence of 0.10 M NaCl and from approximately 50 to approximately 130 nM in solutions of 20 mM 3-(N-morpholino)propanesulfonic acid alone. The regulatory smooth muscle myosin light chain exhibits two equivalent melittin binding sites while each of the others displays only one. The myosin light chains evidently contain elements of structure related to the macromolecular interaction sites present in calmodulin and troponin C but not in parvalbumin. The association of melittin and other peptides with the light chains requires consideration whenever assays of the calmodulin-dependent activity of myosin light chain kinase are used to determine peptide binding by calmodulin. The binding measurements performed on the DTNB light chain and melittin necessitated derivation of the equation relating complex formation to the observed fluorescence anisotropy of a solution containing three fluorescent components. This analysis is generally applicable to equilibria involving the association of two fluorescent molecules emitting in the same wavelength range.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Malencik DA,Anderson SRdoi
10.1021/bi00406a021subject
Has Abstractpub_date
1988-03-22 00:00:00pages
1941-9issue
6eissn
0006-2960issn
1520-4995journal_volume
27pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The ubiquitous calcium-binding protein calmodulin (CaM) regulates a wide variety of cellular events by binding to and activating many distinct target enzymes. The CaM-binding domains of most of these enzymes are contained in a contiguous stretch of amino acids with a length of approximately 20 residues. In this work, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00202a006
更新日期：1994-09-13 00:00:00
abstract：:We have investigated the effects of mixed Na+: Mg2+ ionic solutions on the stability of the nonspecific lac repressor-DNA complex. The effects of Mg2+ are simply interpreted in terms of its role as a competitor (with repressor) for DNA sites. From these studies, the binding constant of the Mg-DNA complex can be determ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00641a005
更新日期：1977-11-01 00:00:00
abstract：:The self-association of rabbit muscle phosphofructokinase at pH 7.0 was investigated by velocity sedimentation. The process was demonstrated to be in a rapid, dynamic equilibrium. The concentration dependence of the weight-average sedimentation coefficient was monitored within the range of 10-750 microgram/mL. The sed...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00513a040
更新日期：1981-05-12 00:00:00
abstract：:Factor VII (proconvertin) has been purified approximately 5 x 10(5)-fold from bovine plasma with an overall yield of 30%. The isolation procedure involves barium sulfate adsorption and elution, DEAE-Sephadex batchwise adsorption and elution, benzamidine-agarose column chromatography, heparin-agarose column chromatogra...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00693a023
更新日期：1975-11-04 00:00:00
abstract：:The increase of the proton motive force mediated by bacteriorhodopsin is compared to the intracellular ATP concentration under steady-state illumination. The membrane potential was measured via the accumulation of the lipophilic ion [14C]triphenylmethylphosphonium and the pH gradient via the accumulation of the weak a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00561a012
更新日期：1980-09-30 00:00:00
abstract：:We describe a reconstitution of light-activated vertebrate photoreceptor GTPase and a purification of the GTP-binding protein (G protein), which is a component of the GTPase and modulates the light-activated phosphodiesterase (PDE) enzyme system. Rod outer segments (ROS) of bull frogs were treated with ethylenediamine...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00528a003
更新日期：1981-12-08 00:00:00
abstract：:The type III secretion system (T3SS) is an essential virulence factor for Shigella flexneri , providing a conduit through which host-altering effectors are injected directly into a host cell to promote uptake. The type III secretion apparatus (T3SA) is composed of a basal body, external needle, and regulatory tip comp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400755f
更新日期：2013-12-10 00:00:00
abstract：:Seven mutant LH1 polypeptides of Rhodobactor sphaeroides have been isolated, and their behaviors in in vitro reconstitution of LH1 and its subunit complex have been characterized. Two mutants were selected to address the increased stability of the subunit complex of Rb. sphaeroides compared with that of Rhodobacter ca...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962386p
更新日期：1997-03-25 00:00:00
abstract：:Dihydrofolate reductase (DHFR) is often used as a model system to study the relation between protein dynamics and catalysis. We have studied a number of variants of the cold-adapted DHFR from Moritella profunda (MpDHFR), in which the catalytically important M20 and FG loops have been altered, and present a comparison ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500508z
更新日期：2014-07-29 00:00:00
abstract：:High-precision studies of oxygen binding in hemoglobin (HbA0) solutions at near-physiological concentrations (2-12 mM heme; pHs 7.0-9.1; various buffers) have led to an unanticipated result: an unmeasurably low contribution from the triply ligated species. We have obtained this result from new differential oxygen-bind...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00387a038
更新日期：1987-06-30 00:00:00
abstract：:Although point mutations usually lead to minor localized changes in protein structure, replacement of conserved Pro-76 with Gly in iso-2-cytochrome c induces a major conformational change. The change in structure results from mutation-induced depression of the pK for transition to an alkaline conformation with altered...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00451a043
更新日期：1989-12-12 00:00:00
abstract：:Although the terms "excessive reactive oxygen species (ROS)" and "oxidative stress" are widely used, the implications of oxidative stress are often misunderstood. ROS are not a single species but a variety of compounds, each with unique biochemical properties and abilities to react with biomolecules. ROS cause activat...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/acs.biochem.6b00703
更新日期：2016-09-27 00:00:00
abstract：:Putative physiological partners of the [2Fe-2S] ferredoxin from Clostridium pasteurianum have been searched by running crude soluble extracts of this bacterium through an affinity column to which the [2Fe-2S] ferredoxin had been covalently bound. Subsequent washing of the column with buffers of increasing ionic streng...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970528p
更新日期：1997-09-30 00:00:00
abstract：:Anatoxin-a and homoanatoxin-a are two potent cyanobacterial neurotoxins. We recently reported the identification of the gene cluster responsible for the biosynthesis of these toxins as well as the in-vitro reconstitution of the first steps of this biosynthesis. We now report experimental evidence supporting the propos...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200892a
更新日期：2011-08-23 00:00:00
abstract：:Biochemical and immunological properties of biosynthetically radiolabeled phosphatidylcholine-(PC-) binding proteins were investigated. The PC-binding proteins were extracted from the detergent lysate of biosynthetically radiolabeled P388D1 cells by affinity chromatography on PC-Sepharose and filtered through a Sephad...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00329a014
更新日期：1985-04-09 00:00:00
abstract：:Rad51 protein, a eukaryotic homologue of RecA protein, forms a filamentous complex with DNA and catalyzes homologous recombination. We have analyzed the structure of Xenopus Rad51 protein (XRad51.1) in solution by small-angle neutron scattering (SANS). The measurements showed that XRad51.1 forms a helical filament ind...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971000n
更新日期：1997-11-04 00:00:00
abstract：:Binding of Ca(2+)-activated calmodulin (Ca(2+)-CaM) to neuronal nitric-oxide synthase (nNOS) increases the rate of 2,6-dichloroindolphenol (DCIP) reduction 2-3-fold and that of cytochrome c(3+) 10-20-fold. Parallel initial velocity patterns indicated that both substrates were reduced via two-half reactions in a ping-p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0023495
更新日期：2001-04-17 00:00:00
abstract：:Efforts to characterize the receptor recognition domain of alpha-macroglobulins have primarily focused on human alpha 2-macroglobulin (alpha 2M). In the present work, the structure and function of the alpha-macroglobulin receptor recognition site were investigated by amino acid sequence analysis, plasma clearance, and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00429a069
更新日期：1989-02-07 00:00:00
abstract：:The ability of cell-penetrating peptides (CPPs) to cross cell membranes has found numerous applications in the delivery of bioactive compounds to the cytosol of living cells. Their internalization mechanisms have been questioned many times, and after 20 years of intense debate, it is now widely accepted that both ener...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi501392n
更新日期：2015-01-20 00:00:00
abstract：:We have examined the ability of the Escherichia coli single-stranded DNA binding protein (SSB) tetramer to form its different binding modes on poly(dC), poly(U), and poly(A) over a range of NaCl and NaF concentrations for comparison with previous studies with poly(dT). In reverse titrations with poly(U) and poly(A) at...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00186a016
更新日期：1994-05-24 00:00:00
abstract：:The origin of the "S3" EPR signal from calcium-depleted photosystem 2 samples has been investigated. This signal is observed after freezing samples under illumination and has been assigned to an interaction between the manganese cluster and an oxidized histidine radical [Boussac et al. (1990) Nature 347; 303-306]. In ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00134a005
更新日期：1992-05-19 00:00:00
abstract：:The toll that Streptococcus pneumoniae exacts on the welfare of humanity is enormous. This organism claims the lives of approximately 3700 people daily, the majority of whom are children below the age of 5, and the situation could worsen due to the increasing incidence of pernicious, multiple-antibiotic-resistant stra...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048075t
更新日期：2004-12-28 00:00:00
abstract：:To investigate the possible role of viral glycoprotein mobility in membrane fusion, fluorescence photobleaching recovery was employed to study the effects of exposure to mildly acidic pH (required to convert many viral fusion proteins to the fusion-active form) on the lateral mobility of influenza hemagglutinin (HA) p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00052a014
更新日期：1993-01-12 00:00:00
abstract：:Several phosphatidylinositol 4,5-bisphosphate (PtdInsP2)-regulated actin-binding proteins and most phosphoinositide-specific phospholipases C (PI-PLCs) comprise a basic amino acid motif (KxxxKxKK, where x denotes any amino acid), which was previously suggested to represent a PtdInsP2-binding site commonly present in t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00015a023
更新日期：1995-04-18 00:00:00
abstract：:3-(Trifluoromethyl)-3-(m-iodophenyl)diazirine (TID) has recently been found to be a noncompetitive inhibitor of the nicotinic acetylcholine receptor (nAcChoR) by both photolabeling and flux assays (White et al., 1991). However, these experiments were done when TID was in equilibrium with the nAcChoR, and thus only its...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00255a018
更新日期：1994-12-27 00:00:00
abstract：:The membrane receptor for immunoglobulin E (IgE) and its ligand, IgE, were irradiated with high-energy electrons. Loss of binding activity was measured for each, and the size of the functional targets was assessed. In both cases, the target size was substantially smaller than the covalent structure of the molecule. Th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00526a011
更新日期：1981-11-10 00:00:00
abstract：:Apurinic/apyrimidinic endonuclease 1 (APE1) is an Mg²⁺-dependent enzyme responsible for incising the DNA backbone 5' to an apurinic/apyrimidinic (AP) site. Here, we use rapid quench flow (RQF) techniques to provide a comprehensive kinetic analysis of the strand-incision activity (k(chemistry)) of APE1 acting on an aut...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401218r
更新日期：2013-10-29 00:00:00
abstract：:Mutations in the gene encoding human ATP:cobalamin adenosyltransferase (hATR) can result in the metabolic disorder known as methylmalonic aciduria (MMA). This enzyme catalyzes the final step in the conversion of cyanocobalamin (vitamin B12) to the essential human cofactor adenosylcobalamin. Here we present the 2.5 A c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061396f
更新日期：2006-12-26 00:00:00
abstract：:Bovine heart ubiquinol-cytochrome c reductase (bc1 complex) was modified with N-(ethoxycarbonyl)-2-ethoxy-1,2-dihydroquinoline (EEDQ), which is a selective reagent for buried carboxyl groups. EEDQ treatment caused a loss of the proton pumping activity of liposome-reconstituted bc1 complex, without effect on the passiv...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9724164
更新日期：1998-02-17 00:00:00
abstract：:The hinge domain encompasses amino acids 51-60 of lactose repressor (LacI) and plays an important role in its regulatory interaction with operator DNA. This segment makes both hinge-DNA and hinge-hinge' contacts that are critical to DNA binding. Furthermore, this small region serves as a central element in communicati...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0114067
更新日期：2001-12-25 00:00:00
