Biochemical properties of phosphatidylcholine-binding proteins that share common antigenic determinants with Fc gamma 2b receptor.

abstract：:Neisseria meningitidis α-1,4-galactosyltransferase C (LgtC) is responsible for the transfer of α-galactose from donor UDP-galactose to the lipooligosaccharide terminal acceptor lactose. Crystal structures of its substrate analogue complexes have provided key insights into the galactosyl transfer mechanism, including a...

journal_title：Biochemistry

authors： Chan PH,Cheung AH,Okon M,Chen HM,Withers SG,McIntosh LP

更新日期：2013-01-15 00:00:00

abstract：:N-Terminal glutaminyl and glutamyl residues of peptides and proteins tend to form pyroglutamic acid (pGlu) by intramolecular cylization. The rate constants for spontaneous cyclization of glutamine (10(-6) s(-1)) and glutamic acid (10(-9) s(-1)) in aqueous solution differ by approximately 3 orders of magnitude at pH 6....

journal_title：Biochemistry

authors： Seifert F,Schulz K,Koch B,Manhart S,Demuth HU,Schilling S

更新日期：2009-12-22 00:00:00

abstract：:Catalysis in protein tyrosine phosphatases (PTPs) involves movement of a protein loop called the WPD loop that brings a conserved aspartic acid into the active site to function as a general acid. Mutation of the tryptophan in the WPD loop of the PTP YopH to any other residue with a planar, aromatic side chain (phenyla...

journal_title：Biochemistry

authors： Moise G,Gallup NM,Alexandrova AN,Hengge AC,Johnson SJ

更新日期：2015-10-27 00:00:00

abstract：:The role of bacterial DnaJ protein as a cochaperone of DnaK is strongly appreciated. Although DnaJ unaccompanied by DnaK can bind unfolded as well as native substrate proteins, its role as an individual chaperone remains elusive. In this study, we demonstrate that DnaJ binds a model non-native substrate with a low nan...

journal_title：Biochemistry

authors： Tiwari S,Kumar V,Jayaraj GG,Maiti S,Mapa K

更新日期：2013-02-12 00:00:00

abstract：:Diverse voltage-gated K+ channels are produced by tetramerization of channel alpha subunits that can be encoded by multiple genes. In GH3 pituitary cells, the Kv1.4 and Kv1.5 subunit genes are constitutively expressed, and dexamethasone selectively up-regulates expression of the latter gene. Here we use subunit-specif...

journal_title：Biochemistry

authors： Takimoto K,Levitan ES

更新日期：1996-11-12 00:00:00

abstract：:Specific binding of glutamate to its carrier was investigated by using cytoplasmic membrane vesicles prepared from Escherichia coli B. The binding activity was specifically affected by the Na+ and H+ concentrations of the medium. Cytoplasmic membrane vesicles from the mutant strain 36-39 that is defective in the Na+-d...

journal_title：Biochemistry

authors： Fujimura T,Yamato I,Anraku Y

更新日期：1983-04-12 00:00:00

abstract：:Upon injury of a blood vessel, activated factor VII (FVIIa) forms a high-affinity complex with its allosteric regulator, tissue factor (TF), and initiates blood clotting. Active site-inhibited factor VIIa (FVIIai) binds to TF with even higher affinity. We compared the interactions of FVIIai and FVIIa with soluble TF (...

journal_title：Biochemistry

authors： Osterlund M,Owenius R,Carlsson K,Carlsson U,Persson E,Lindgren M,Freskgård PO,Svensson M

更新日期：2001-08-07 00:00:00

abstract：:In the ras gene superfamily, codon 12 (-TGGTG-) of the K-ras gene is the most frequently mutated codon in human cancers. Recently, we have found that bulky chemical carcinogens preferentially form DNA adducts at codons 12 and 14 (-CGTAG-) in the K-ras gene in normal human bronchial epithelial (NHBE) cells. Furthermore...

journal_title：Biochemistry

authors： Hu W,Feng Z,Tang MS

更新日期：2003-08-26 00:00:00

abstract：:The sequence of two overlapping cDNA clones for the zinc metalloproteinase hemorrhagic toxin e (also known as atrolysin e, EC 3.4.24.44) from the venom gland of Crotalus atrox, the Western diamondback rattlesnake, is presented. The assembled cDNA sequence is 1975 nucleotides in length and encodes an open reading frame...

journal_title：Biochemistry

authors： Hite LA,Shannon JD,Bjarnason JB,Fox JW

更新日期：1992-07-14 00:00:00

abstract：:Doxorubicin (DOX) is a chemotherapeutic that is used in the treatment of a wide variety of cancers. However, it causes cardiotoxicity partly because of the formation of reactive oxygen species. CYP2J2 is a human cytochrome P450 that is strongly expressed in cardiomyocytes. It converts arachidonic acid (AA) into four d...

journal_title：Biochemistry

authors： Arnold WR,Baylon JL,Tajkhorshid E,Das A

更新日期：2017-12-26 00:00:00

abstract：:The effect of mutating Gly 478 to Ala in rat cytochrome P450 2B1 on the metabolism of N-benzyl-1-aminobenzotriazole was investigated. The 7-ethoxy-4-(trifluoromethyl)coumarin O-deethylation activity of the wild-type enzyme was completely inactivated by incubating with 1 microM BBT. The G478A mutant, however, was not i...

journal_title：Biochemistry

authors： Kent UM,Hanna IH,Szklarz GD,Vaz AD,Halpert JR,Bend JR,Hollenberg PF

更新日期：1997-09-30 00:00:00

abstract：:Escherichia coli (A)BC excinuclease is the major enzyme responsible for removing bulky adducts, such as pyrimidine dimers and 6-4 photoproducts, from DNA. Mutants deficient in this enzyme are extremely sensitive to UV and UV-mimetic agents, but not to oxidizing agents, or ionizing radiation which damages DNA in part b...

journal_title：Biochemistry

authors： Lin JJ,Sancar A

更新日期：1989-10-03 00:00:00

abstract：:Rabbit muscle lactate dehydrogenase (LDH) was coupled to Sepharose in such a way that each molecule is expected to be attached via only one subunit. Dissociation of the bound active enzyme by several methods all yielded immobilized subunit derivatives which were inactive. These derivatives were capable of regenerating...

journal_title：Biochemistry

authors： Chan WW,Mosbach K

更新日期：1976-09-21 00:00:00

abstract：:Our recently reported pressure-jump relaxation kinetics experiments on staphylococcal nuclease folding and unfolding [Vidugiris et al. (1995) Biochemistry 34, 4909] demonstrated that both transitions exhibit positive activation volumes, with that of folding being much larger than that of unfolding. Thus high pressure ...

journal_title：Biochemistry

authors： Vidugiris GJ,Truckses DM,Markley JL,Royer CA

更新日期：1996-03-26 00:00:00

abstract：:It is well-known, but little understood, that the nucleotide sequences between phased A(4-6)-tracts (at 10-11 bp intervals) have only a slight effect on overall curvature. To explore this phenomenon, we have examined the gel-migration properties of sequences containing both A-tracts as well as G-tracts (i.e., sequence...

journal_title：Biochemistry

authors： Merling A,Sagaydakova N,Haran TE

更新日期：2003-05-06 00:00:00

abstract：:Transcripts of Saccharomyces cerevisiae nuclear tRNA genes are normally terminated within a few nucleotides of the tRNA coding region, in contrast to mitochondrially encoded tRNAs, which are contained within polycistronic transcripts and thus require 3'-processing by mitochondrial endonucleases. We show that 3'-proces...

journal_title：Biochemistry

authors： Furter R,Snaith M,Gillespie DE,Hall BD

更新日期：1992-11-10 00:00:00

abstract：:myo-Inositol oxygenase (MIOX) uses iron as its cofactor and dioxygen as its cosubstrate to effect the unique, ring-cleaving, four-electron oxidation of its cyclohexan-(1,2,3,4,5,6-hexa)-ol substrate to d-glucuronate. The nature of the iron cofactor and its interaction with the substrate, myo-inositol (MI), have been p...

journal_title：Biochemistry

authors： Xing G,Hoffart LM,Diao Y,Prabhu KS,Arner RJ,Reddy CC,Krebs C,Bollinger JM Jr

更新日期：2006-05-02 00:00:00

abstract：:The binding of Zn2+ to tubulin and the ability of this cation to promote the polymorphic assembly of the protein were examined. Equilibrium binding showed the existence of more than 60 potential Zn2+ binding sites on the dimer, including a number of high-affinity sites. The number of high-affinity sites, estimated by ...

journal_title：Biochemistry

authors： Eagle GR,Zombola RR,Himes RH

更新日期：1983-01-04 00:00:00

abstract：:Sensory rhodopsin I (SRI) is a seven-transmembrane helix retinylidene protein that mediates color-sensitive phototaxis responses through its bound transducer HtrI in the archaeon Halobacterium salinarum. Deprotonation of the Schiff base attachment site of the chromophore accompanies formation of the SRI signaling stat...

journal_title：Biochemistry

authors： Jung KH,Spudich EN,Dag P,Spudich JL

更新日期：1999-10-05 00:00:00

abstract：:Sensory rhodopsin II (SRII) is unique among the archaeal rhodopsins in having an absorption maximum near 500 nm, blue shifted roughly 70 nm from the other pigments. In addition, SRII displays vibronic structure in the lambda(max) absorption band, whereas the other pigments display fully broadened band maxima. The mole...

journal_title：Biochemistry

authors： Ren L,Martin CH,Wise KJ,Gillespie NB,Luecke H,Lanyi JK,Spudich JL,Birge RR

更新日期：2001-11-20 00:00:00

abstract：:We have analyzed by band-shift assays HeLa cell protein-DNA interactions on a stable cruciform DNA molecule. The stable cruciform was formed by heteroduplexing the HindIII-SphI fragment of SV40 virus DNA that contains the origin of replication with a derivative mutant containing a heterologous substitution at the cent...

journal_title：Biochemistry

authors： Pearson CE,Ruiz MT,Price GB,Zannis-Hadjopoulos M

更新日期：1994-11-29 00:00:00

abstract：:Proteins from thermophiles possess high thermostability. The stabilization mechanisms differ between archaeal and bacterial proteins, whereby archaeal proteins are mainly stabilized via hydrophobic interactions and bacterial proteins by ion pairs. High stability is an important factor in promoting protein evolution, b...

journal_title：Biochemistry

authors： Takano K,Aoi A,Koga Y,Kanaya S

更新日期：2013-07-16 00:00:00

abstract：:While the insertion of a hydrophobic peptide or membrane protein segment into the bilayer can be spontaneous and driven mainly by the hydrophobic effect, anionic lipids, which comprise ca. 20% of biological membranes, provide a source of electrostatic attractions for binding of proteins/peptides into membranes. To unr...

journal_title：Biochemistry

authors： Liu LP,Deber CM

更新日期：1997-05-06 00:00:00

abstract：:Properties of the interaction of bleomycin (Blm) and metallobleomycins [M = Zn, Cu(II), Fe(III), and HO(2)-Co(III)] with site-specific and nonspecific DNA oligomers, d(GGAAGCTTCC)(2) (I) and d(GGAAATTTCC)(2) (II), respectively, were investigated. With both 10-mers association constants increased in the series Blm A(2)...

journal_title：Biochemistry

authors： Li W,Zhao C,Xia C,Antholine WE,Petering DH

更新日期：2001-06-26 00:00:00

abstract：:The structure and dynamic properties of the C-terminal region of the human class alpha glutathione transferase A1-1 have been investigated with high-resolution NMR methods. On the basis of crystallographic and fluorescence measurements, this 13-residue segment of the enzyme is presumed to be disordered in the unligand...

journal_title：Biochemistry

authors： Zhan Y,Rule GS

更新日期：2004-06-15 00:00:00

abstract：:The UV endonucleases [endodeoxyribonuclease (pyrimidine dimer), EC 3.1.25.1] from Micrococcus luteus and bacteriophage T4 possess two catalytic activities specific for the site of cyclobutane pyrimidine dimers in UV-irradiated DNA: a DNA glycosylase that cleaves the 5'-glycosyl bond of the dimerized pyrimidines and an...

journal_title：Biochemistry

authors： Liuzzi M,Weinfeld M,Paterson MC

更新日期：1987-06-16 00:00:00

abstract：:How various anti-amyloidogenic compounds inhibit the formation of Alzheimer's beta-amyloid fibrils (fAbeta) from amyloid beta-peptide (Abeta) and destabilize fAbeta remains poorly understood. Using spectrophotometry, spectrofluorometry, atomic force microscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis...

journal_title：Biochemistry

authors： Hirohata M,Hasegawa K,Tsutsumi-Yasuhara S,Ohhashi Y,Ookoshi T,Ono K,Yamada M,Naiki H

更新日期：2007-02-20 00:00:00

abstract：:Mitochondrial thiolase I from pig heart has been found to have at least two and possibly three reactive sulfhydryl residues at or near the active site [Izbicka-Dimitrijević, E., & Gilbert, H. F. (1982) Biochemistry 21, 6112-6118; Izbicka-Dimitrijević, E., & Gilbert, H. F. (1984) Biochemistry 23, 4318-4324]. In the nat...

journal_title：Biochemistry

authors： Izbicka E,Gilbert HF

更新日期：1984-12-18 00:00:00

abstract：:Hepatic microsomal NADPH-cytochrome P-450 reductase was solubilized from rabbit liver microsomes in the presence of detergents and purified to homogeneity by column chromatography. The purified reductase had a molecular weight of 78 000 and contained 1 mol each of FAD and FMN per mol of enzyme. On reduction with NADPH...

journal_title：Biochemistry

authors： Iyanagi T,Anan FK,Imai Y,Mason HS

更新日期：1978-05-30 00:00:00

abstract：:Intrastrand cross-links represent the majority of modifications in DNA resulting from interaction with the cancer chemotherapeutic drug cis-diamminedichloroplatinum(II) (cis-DDP). These adducts were recently characterized although several discrepancies remained to be resolved. In these studies, [3H]-cis-dichloro(ethyl...

journal_title：Biochemistry

authors： Eastman A

更新日期：1986-07-01 00:00:00