Abstract:
:Hepatic microsomal NADPH-cytochrome P-450 reductase was solubilized from rabbit liver microsomes in the presence of detergents and purified to homogeneity by column chromatography. The purified reductase had a molecular weight of 78 000 and contained 1 mol each of FAD and FMN per mol of enzyme. On reduction with NADPH in the presence of molecular oxygen, an 02-stable semiquinone containing one flavin free radical per two flavins was formed, in agreement with previous work on purified trypsin-solubilized reductase. The reduction of oxidized enzyme by NADPH, and autoxidation of NADPH-reduced enzyme by air, proceeded by both one-electron equivalent and two-electron equivalent mechanisms. The reductase reduced cytochrome P-450 (from phenobarbital-treated rabbits) and cytochrome P-448 (from 3-methylcholanthrene-treated rabbits). The rate of reduction of cytochrome P-450 increased in the presence of a substrate, benzphetamine, but that of cytochrome P-448 did not.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Iyanagi T,Anan FK,Imai Y,Mason HSdoi
10.1021/bi00604a032subject
Has Abstractpub_date
1978-05-30 00:00:00pages
2224-30issue
11eissn
0006-2960issn
1520-4995journal_volume
17pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Imino proton resonances in the downfield region (10-14 ppm) of the 500-MHz 1H NMR spectrum of Torulopsis utilis 5S RNA are identified (A X U, G X C, or G X U) and assigned to base pairs in helices I, IV, and V via analysis of homonuclear Overhauser enhancements (NOE) from intact T. utilis 5S RNA, its RNase T1 and RNas...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00366a021
更新日期:1986-09-09 00:00:00
abstract::We have used a photochemical method to map covalent binding sites of the carcinogen benzo[a]pyrenediol epoxide (BPDE) within DNA from the transcriptional control region of the chicken adult beta-globin gene. Our preliminary low-resolution mapping has demonstrated that this region contains highly preferred BPDE binding...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00358a045
更新日期:1986-05-20 00:00:00
abstract::The allosteric activation of the T127-->L mutant of 3',5'-cyclic adenosine monophosphate (cAMP) receptor protein (CRP) by cAMP changes from an exothermic, independent two-site binding mechanism at pH 7.0 to an endothermic, interacting two-site binding mechanism at pH 5.2, similar to that observed for CRP at pH 7.0 and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000225m
更新日期:2000-06-20 00:00:00
abstract::The calcium sensitivity of vertebrate skeletal muscle myosin has been investigated. Adenosinetriphosphatase (ATPase) activity was assayed in a reconstituted system composed of either purified rabbit myosin plus actin or myosin plus actin, tropomyosin, and troponin. The calcium sensitivity of actomyosin Mg-ATPase activ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00279a004
更新日期:1983-05-10 00:00:00
abstract::Heme uptake and utilization by pathogenic bacteria are critical for virulence and disease, since heme and heme proteins are a major source of iron within the host. Although the role of outer membrane heme receptors in this process has been extensively characterized at the genetic and biochemical level, the role of the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061722r
更新日期:2007-03-20 00:00:00
abstract::An endonuclease II preparation from Escherichia coli makes single strand breaks in DNA which has been treated with the carcinogen 7-bromomethyl-12-methylbenz[alpha]anthracene. In addition, the enzyme preparation excises N6-(12-methylbenz[alpha]anthracenyl-7-methyl)adenine and N2-(12-methylbenz[alpha]anthracenyl-7-met...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00697a002
更新日期:1975-12-30 00:00:00
abstract::The nitrophorins are a family of proteins that use ferric heme to transport nitric oxide (NO) from the salivary glands of blood-sucking insects to their victims, resulting in vasodilation and reduced blood coagulation. We have refined atomic resolution structures of nitrophorin 4 (NP4) from Rhodnius prolixus complexed...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0109257
更新日期:2001-09-25 00:00:00
abstract::To understand the role of disulfide bridges in protein stability, the thermodynamic changes in the denaturation of two mutant human lysozymes lacking a disulfide bridge between Cys-77 and Cys-95 (C77A and C77/95A) were analyzed using differential scanning calorimetry (DSC). At pH 3.0 and 57 degrees C, the stabilities ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00150a028
更新日期:1992-09-08 00:00:00
abstract::The interactions of Alzheimer's amyloid beta-peptide with cyclodextrins were studied by (1)H NMR: the translational diffusion coefficient of the peptide and chemical shift changes were studied by the presence of variable concentrations of cyclodextrins. For the full-length peptide, Abeta(1-40), the combined results of...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036254p
更新日期:2004-05-25 00:00:00
abstract::The binding of the inhibitor 8-nitroxanthine to urate oxidase has been investigated by Raman and UV-visible absorption spectroscopy. The absorption maximum of 8-nitroxanthine shifts from 380 to 400 nm upon binding to the enzyme, demonstrating that the electronic structure of the ligand is perturbed. It has been propos...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0507837
更新日期:2005-08-30 00:00:00
abstract::The melanocortin-4 (MC4) receptor is a potential therapeutic target for obesity and cachexia, for which nonpeptide agonists and antagonists are being developed, respectively. The aim of this study was to identify molecular interactions between the MC4 receptor and nonpeptide ligands, and to compare the mechanism of bi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051316s
更新日期:2005-11-08 00:00:00
abstract::The LAGLIDADG homing endonucleases include free-standing homodimers, pseudosymmetric monomers, and related enzyme domains embedded within inteins. DNA-bound structures of homodimeric I-CreI and monomeric I-SceI indicate that three catalytic divalent metal ions are distributed across a pair of overlapping active sites,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048970c
更新日期:2004-11-09 00:00:00
abstract::CDP-ribitol synthase is a bifunctional reductase and cytidylyltransferase that catalyzes the transformation of D-ribulose 5-phosphate, NADPH, and CTP to CDP-ribitol, a repeating unit present in the virulence-associated polysaccharide capsules of Haemophilus influenzae types a and b [Follens, A., et al. (1999) J. Bacte...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002745n
更新日期:2001-04-24 00:00:00
abstract::Calmodulin forms a covalent, one to one, complex with 3H-labeled norchlorpromazine isothiocyanate. Complex formation was monitored by high-performance liquid chromatography using a CN reverse-phase column which resolves calmodulin, the calmodulin-norchlorpromazine adduct, and norchlorpromazine isothiocyanate. Formatio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00293a003
更新日期:1983-11-22 00:00:00
abstract::Protein misfolding and aggregation is a ubiquitous phenomenon associated with a wide range of diseases. The synuclein family comprises three small naturally unfolded proteins implicated in neurodegenerative diseases and some forms of cancer. α-Synuclein is a soluble protein that forms toxic inclusions associated with ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300478w
更新日期:2012-06-12 00:00:00
abstract::Prosthetic groups such as heme, chlorophyll, and cobalamin (vitamin B(12)) are characterized by their branched biosynthetic pathway and unique metal insertion steps. The metal ion chelatases can be broadly classed either as single-subunit ATP-independent enzymes, such as the anaerobic cobalt chelatase and the protopor...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9906773
更新日期:1999-08-17 00:00:00
abstract::Thermodynamic parameters of helix formation were measured spectroscopically for 16 oligoribonucleotides containing either internal GU mismatches or the corresponding AU pairs. Internal GU mismatches stabilize each helix, but not as much as the corresponding AU pairs. The differences in the enthalpy and entropy changes...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00367a061
更新日期:1986-09-23 00:00:00
abstract::DnaB helicase is responsible for unwinding duplex DNA during chromosomal DNA replication and is an essential component of the DNA replication apparatus in Escherichia coli. We have analyzed the mechanism of binding of single-stranded DNA (ssDNA) by the DnaB x DnaC complex and DnaB helicase. Binding of ssDNA to DnaB he...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060118d
更新日期:2006-09-26 00:00:00
abstract::Cyanobacteriochromes (CBCRs) are cyanobacterial photoreceptors that exhibit photochromism between two states: a thermally stable dark-adapted state and a metastable light-adapted state with bound linear tetrapyrrole (bilin) chromophores possessing 15Z and 15E configurations, respectively. The photodynamics of canonica...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00796
更新日期:2021-01-13 00:00:00
abstract::Ion channel peptides have been prepared by solid-phase peptide synthesis, labeled with 15N at selected sites, and reconstituted into oriented lipid bilayers. The (Leu-Ser-Ser-Leu-Leu-Ser-Leu)3-CONH2 peptide has previously been shown to exhibit well-defined and discrete ionic conductances when investigated by single-ch...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050866n
更新日期:2005-09-13 00:00:00
abstract::The highly cooperative binding of fd gene 5 to single-stranded DNA was studied kinetically by rapid photo-cross-linking and stopped-flow UV absorption measurements. The observed change in absorbance was shown to be due to the binding by direct evidence of rapid photo-cross-linking of the bound proteins to fd DNA. The ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00294a028
更新日期:1983-12-06 00:00:00
abstract::The tertiary structure of apolipoprotein (apo) A-I and the contributions of structural domains to the properties of the protein molecule are not well defined. We used a series of engineered human and mouse apoA-I molecules in a range of physical-biochemical measurements to address this issue. Circular dichroism measur...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi702332b
更新日期:2008-02-19 00:00:00
abstract::Typical of many transcriptional regulatory proteins, the lambdoid bacteriophage repressors bind cooperatively to multiple sites on DNA. This cooperative binding is essential for establishment and maintenance of phage lysogeny. In the phage, two repressor homodimers, one bound at each of the adjacent operator sites, in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi027318j
更新日期:2003-04-15 00:00:00
abstract::Serine transacetylase from Salmonella typhimurium was purified to near homogeneity. A complete initial velocity study in both reaction directions suggests that the enzyme catalyzes the conversion of acetyl CoA and L-serine to O-acetyl-L-serine (OAS) and coenzyme A (CoASH) by a ping pong bi bi kinetic mechanism. Initia...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00175a040
更新日期:1994-03-08 00:00:00
abstract::When grown under O2-limited conditions, Escherichia coli expresses a cytochrome bd quinol oxidase that has an unusually high affinity for O2. We have studied the reaction of cytochrome bd with CO and O2 by rapid-reaction spectrophotometry. The reduced enzyme forms a photosensitive ferrocytochrome d-CO complex, and fol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00254a021
更新日期:1994-12-20 00:00:00
abstract::The cytochrome d complex is a two-subunit, membrane-bound terminal oxidase in the aerobic respiratory chain of Escherichia coli. The enzyme catalyzes the two-electron oxidation of ubiquinol and the four-electron reduction of oxygen to water. Previous work demonstrated that the site for ubiquinol oxidation was selectiv...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00228a007
更新日期:1991-04-09 00:00:00
abstract::Previous studies of Escherichia coli dihydrofolate reductase (ecDHFR) have demonstrated that residue G121, which is 19 A from the catalytic center, is involved in catalysis, and long distance dynamical motions were implied. Specifically, the ecDHFR mutant G121V has been extensively studied by various experimental and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0518242
更新日期:2006-02-07 00:00:00
abstract::The unfolding and refolding properties of human lysozyme and two amyloidogenic variants (Ile56Thr and Asp67His) have been studied by stopped-flow fluorescence and hydrogen exchange pulse labeling coupled with mass spectrometry. The unfolding of each protein in 5.4 M guanidine hydrochloride (GuHCl) is well described as...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi983037t
更新日期:1999-05-18 00:00:00
abstract::The C2 domain is a Ca2+-dependent, membrane-targeting motif originally discovered in protein kinase C and recently identified in numerous eukaryotic signal-transducing proteins, including cytosolic phospholipase A2 (cPLA2) of the vertebrate inflammation pathway. Intracellular Ca2+ signals recruit the C2 domain of cPLA...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9717340
更新日期:1997-10-07 00:00:00
abstract::Epidermal growth factor receptor (EGFR) exemplifies the family of receptor tyrosine kinases that mediate numerous cellular processes, including growth, proliferation, and differentiation. Moreover, gene amplification and EGFR mutations have been identified in a number of human malignancies, making this receptor an imp...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi301441e
更新日期:2012-12-18 00:00:00