The room temperature reaction of carbon monoxide and oxygen with the cytochrome bd quinol oxidase from Escherichia coli.


:When grown under O2-limited conditions, Escherichia coli expresses a cytochrome bd quinol oxidase that has an unusually high affinity for O2. We have studied the reaction of cytochrome bd with CO and O2 by rapid-reaction spectrophotometry. The reduced enzyme forms a photosensitive ferrocytochrome d-CO complex, and following photolysis, CO recombines with the reduced enzyme with a bimolecular rate of 8 x 10(7) M-1 s-1. Reaction of CO-bound enzyme with O2 gives a CO off-rate of 1.6 s-1. The O2 reaction is followed by a flow-flash procedure in which CO-ligated enzyme is mixed with O2, and the reaction commenced by photolysis of cytochrome d-CO. In the presence of O2, two processes are resolved on a time-scale of 300 microseconds. The absorbance at 645 nm first increases at a rate that is dependent on O2 concentration with a value of 2 x 10(9) M-1 s-1. The second phase results in decreased absorbance at 645 nm and increased absorbance at 680 nm. The rate of the second process is independent from O2 concentration above 50 microM O2 and reaches a first-order limit of 1 x 10(4) s-1. A model for the reaction of the cytochrome bd quinol oxidase with O2 is proposed in which an initial ferrocytochrome d-oxy adduct forms, and then decays to a ferryl-oxo species. The oxidation of the low-spin cytochrome b component of the oxidase, monitored at 560 nm, occurs at the same time as the ferryl species forms. We suggest that the suitability of the cytochrome bd quinol oxidase to function at low O2 concentration is conferred by its rapid rate of binding O2.






Hill BC,Hill JJ,Gennis RB




Has Abstract


1994-12-20 00:00:00












  • Post-translational modifications of aquaporin 0 (AQP0) in the normal human lens: spatial and temporal occurrence.

    abstract::Because of the lack of protein turnover in fiber cells of the ocular lens, Aquaporin 0 (AQP0), the most abundant membrane protein in the lens, undergoes extensive post-translational modification with fiber cell age. To map the distribution of modified forms of AQP0 within the lens, normal human lenses ranging in age f...


    pub_type: 杂志文章


    authors: Ball LE,Garland DL,Crouch RK,Schey KL

    更新日期:2004-08-03 00:00:00

  • Translation of collagen messenger RNA in a cell-free system derived from wheat germ.

    abstract::A cell-free system for synthesizing protein from wheat germ was used to translate the messenger RNA extracted from 16-day embryonic chick calvaria. A part of the product had properties similar to collagenous peptides and served as a substrate for prolyl hydroxylase, an enzyme specific for collagen. The level of potass...


    pub_type: 杂志文章


    authors: Benveniste K,Wilczek J,Ruggieri A,Stern R

    更新日期:1976-02-24 00:00:00

  • A mutational analysis of binding interactions in an antigen-antibody protein-protein complex.

    abstract::Alanine scanning mutagenesis, double mutant cycles, and X-ray crystallography were used to characterize the interface between the anti-hen egg white lysozyme (HEL) antibody D1.3 and HEL. Twelve out of the 13 nonglycine contact residues on HEL, as determined by the high-resolution crystal structure of the D1.3-HEL comp...


    pub_type: 杂志文章


    authors: Dall'Acqua W,Goldman ER,Lin W,Teng C,Tsuchiya D,Li H,Ysern X,Braden BC,Li Y,Smith-Gill SJ,Mariuzza RA

    更新日期:1998-06-02 00:00:00

  • Intercalating polycyclic aromatic hydrocarbon-DNA adducts poison DNA religation by Vaccinia topoisomerase and act as roadblocks to digestion by exonuclease III.

    abstract::Polycyclic aromatic hydrocarbon (PAH)-DNA adducts pervert the execution or fidelity of enzymatic DNA transactions and cause mutations and cancer. Here, we examine the effects of intercalating PAH-DNA adducts on the religation reaction of vaccinia DNA topoisomerase, a prototypal type IB topoisomerase (TopIB), and the 3...


    pub_type: 杂志文章


    authors: Yakovleva L,Handy CJ,Yagi H,Sayer JM,Jerina DM,Shuman S

    更新日期:2006-06-20 00:00:00

  • Resonance energy transfer as a direct monitor of GTP-binding protein-effector interactions: activated alpha-transducin binding to the cGMP phosphodiesterase in the bovine phototransduction cascade.

    abstract::Resonance energy-transfer approaches have been used to directly monitor the interactions of the GTP gamma S-bound alpha subunit of transducin (alpha T GTP gamma S) with the retinal cyclic GMP phosphodiesterase (PDE). The PDE was labeled with 5-(iodoacetamido) fluorescein (IAF-PDE) and served as the fluorescence donor ...


    pub_type: 杂志文章


    authors: Erickson JW,Cerione RA

    更新日期:1991-07-23 00:00:00

  • Transbilayer exchange of phosphatidylethanolamine for phosphatidylcholine and N-acetimidoylphosphatidylethanolamine in single-walled bilayer vesicles.

    abstract::A preparation of small single-walled liposome vesicles containing a 9:1 mole ratio of phosphatidylcholine to phosphatidylethanolamine was subjected to reaction with the membrane-impermeable reagent, isethionyl acetimidate hydrochloride. This reagent converted 90% of the external phosphatidylethanolamine groups to the ...


    pub_type: 杂志文章


    authors: Roseman M,Litman BJ,Thompson TE

    更新日期:1975-11-04 00:00:00

  • Electrostatic Constituents of the Interaction of Cardiolipin with Site A of Cytochrome c.

    abstract::Cytochrome c binds to cardiolipin (CL) on the inner mitochondrial membrane during the initial stages of apoptosis where it oxidizes CL, promoting its release into the cytoplasm where it initiates apoptosis. Previous work has identified interaction sites on cytochrome c involved in the cytochrome c-CL interaction. The ...


    pub_type: 杂志文章


    authors: Elmer-Dixon MM,Bowler BE

    更新日期:2018-10-02 00:00:00

  • Calcium modulates the photoassembly of photosystem II (Mn)4-clusters by preventing ligation of nonfunctional high-valency states of manganese.

    abstract::The requirement for Ca2+ in the Mn(2+)-dependent photoactivation of oxygen evolution was re-evaluated using 17 kDa/24 kDa-less photosystem II (PSII) membranes depleted of (Mn)4-clusters by NH2OH extraction. At optimum conditions (1 mM Mn2+/10 microM 2,6-dichlorphenolindophenol (DCIP)/20 mM Ca2+), the light-induced inc...


    pub_type: 杂志文章


    authors: Chen C,Kazimir J,Cheniae GM

    更新日期:1995-10-17 00:00:00

  • Purification and properties of acyl coenzyme A thioesterase II from Rhodopseudomonas sphaeroides.

    abstract::A high molecular weight acyl coenzyme A (acyl-CoA) thioesterase, designated thioesterase II, has been purified 5300-fold from photoheterotrophically grown cells of Rhodopseudomonas sphaeroides. In contrast to R. sphaeroides acyl-CoA thioesterase I [Boyce, S.G., & Lueking, D.R. (1984) Biochemistry 23, 141-147], thioest...


    pub_type: 杂志文章


    authors: Seay T,Lueking DR

    更新日期:1986-05-06 00:00:00

  • Identification of a new class of inhibitors of the voltage-gated potassium channel, Kv1.3, with immunosuppressant properties.

    abstract::The voltage-gated potassium channel, K(v)1.3, is a novel target for development of immunosuppressants. Using a functional (86)Rb(+) efflux assay, a new class of high-affinity K(v)1.3 inhibitors has been identified. The initial active in this series, 4-phenyl-4-[3-(2-methoxyphenyl)-3-oxo-2-azaprop-1-yl]cyclohexanone (P...


    pub_type: 杂志文章


    authors: Schmalhofer WA,Bao J,McManus OB,Green B,Matyskiela M,Wunderler D,Bugianesi RM,Felix JP,Hanner M,Linde-Arias AR,Ponte CG,Velasco L,Koo G,Staruch MJ,Miao S,Parsons WH,Rupprecht K,Slaughter RS,Kaczorowski GJ,Garcia ML

    更新日期:2002-06-18 00:00:00

  • Investigation of the catalytic mechanism of yeast inorganic pyrophosphatase.

    abstract::P1,P2-Bidentate Co(NH3)4PP was found to be a competitive inhibitor of pyrophosphatase vs. MgPP (Kis = 8.7 mM, pH 7) and, in the presence of Mg2+, an active substrate as well. P1,P2-Bidentate Cr(III) complexes of pyrophosphate, imidodiphosphate, and methylenediphosphonate were also competitive inhibitors vs. MgPP (pH 5...


    pub_type: 杂志文章


    authors: Knight WB,Fitts SW,Dunaway-Mariano D

    更新日期:1981-07-07 00:00:00

  • Enhanced protein thermostability by Ala-->Aib replacement.

    abstract::The introduction into peptide chains of alpha-aminoisobutyric acid (Aib) has proven to stabilize the helical structure in short peptides by restricting the available range of polypeptide backbone conformations. In order to evaluate the potential stabilizing effect of Aib at the protein level, we have studied the confo...


    pub_type: 杂志文章


    authors: De Filippis V,De Antoni F,Frigo M,Polverino de Laureto P,Fontana A

    更新日期:1998-02-10 00:00:00

  • Structural principles for the multispecificity of small GTP-binding proteins.

    abstract::The functional diversity of small GTP-binding proteins (G proteins) and their ability to function as molecular switches are based on their interactions with many different proteins. A wealth of structural data has revealed that their partners are often unrelated to each other in sequence and structure, but their bindi...


    pub_type: 杂志文章


    authors: Biou V,Cherfils J

    更新日期:2004-06-08 00:00:00

  • G Protein betagamma dimer formation: Gbeta and Ggamma differentially determine efficiency of in vitro dimer formation.

    abstract::The Gbeta and Ggamma subunit of the heterotrimeric G proteins form a functional dimer that is stable once assembled in vivo or in vitro. The requirements, mechanism, and specificity of dimer formation are still incompletely understood, but represent important biochemical processes involved in the specificity of cellul...


    pub_type: 杂志文章


    authors: Dingus J,Wells CA,Campbell L,Cleator JH,Robinson K,Hildebrandt JD

    更新日期:2005-09-06 00:00:00

  • Heteronuclear 3D NMR studies of water bound to an FK506 binding protein/immunosuppressant complex.

    abstract::From a series of 15N-resolved 3D ROESY-HMQC and 13C-resolved 3D NOESY-HMQC spectra of the FK506 binding protein (FKBP)/ascomycin complex in H2O, the locations of three tightly bound water molecules were identified. These waters are all buried within the interior of the complex and form an integral part of its structur...


    pub_type: 杂志文章


    authors: Xu RX,Meadows RP,Fesik SW

    更新日期:1993-03-16 00:00:00

  • Rat organic anion transporting protein 1A1 (Oatp1a1): purification and phosphopeptide assignment.

    abstract::Rat organic anion transporting protein 1a1 (oatp1a1), a hepatocyte basolateral plasma membrane protein, mediates transport of various amphipathic compounds. Our previous studies indicated that serine phosphorylation of a single tryptic peptide inhibits its transport activity without changing its cell surface content. ...


    pub_type: 杂志文章


    authors: Xiao Y,Nieves E,Angeletti RH,Orr GA,Wolkoff AW

    更新日期:2006-03-14 00:00:00

  • Structural and functional effects of tryptophans inserted into the membrane-binding and substrate-binding sites of human group IIA phospholipase A2.

    abstract::Phospholipase A(2) (PLA(2)) enzymes become activated by binding to biological membranes and hydrolyze phospholipids to free fatty acids and lyso-phospholipids, the precursors of inflammatory mediators. To understand the functional significance of amino acid residues at key positions, we have studied the effects of the...


    pub_type: 杂志文章


    authors: Nemec KN,Pande AH,Qin S,Bieber Urbauer RJ,Tan S,Moe D,Tatulian SA

    更新日期:2006-10-17 00:00:00

  • Evidence for the amphipathic nature and tilted topology of helices 4 and 5 in the closed state of the colicin E1 channel.

    abstract::The membrane-bound closed channel structure of helices 4 and 5 (Lys-406-Asp-446) of the colicin E1 channel domain was investigated by using a site-directed fluorescence labeling technique. A bimane probe was covalently attached to a cysteine residue in a series of single-cysteine mutant proteins to scan each helix in ...


    pub_type: 杂志文章


    authors: Ho D,Merrill AR

    更新日期:2009-02-17 00:00:00

  • Macrophage oxidation of L-arginine to nitrite and nitrate: nitric oxide is an intermediate.

    abstract::Previous studies have shown that murine macrophages immunostimulated with interferon gamma and Escherichia coli lipopolysaccharide synthesize NO2-, NO3-, and citrulline from L-arginine by oxidation of one of the two chemically equivalent guanido nitrogens. The enzymatic activity for this very unusual reaction was foun...


    pub_type: 杂志文章


    authors: Marletta MA,Yoon PS,Iyengar R,Leaf CD,Wishnok JS

    更新日期:1988-11-29 00:00:00

  • Calorimetric studies on the interaction of horse ferricytochrome c and yeast cytochrome c peroxidase.

    abstract::The binding of horse ferricytochrome c to yeast cytochrome c peroxidase at pH 6.0 in 8.7 mM phosphate buffer (0.0100 M ionic strength) is characterized by a small, unfavorable enthalpy change (+1.91 +/- 0.16 kcal mol-1) and a large, positive entropy change (+37 +/- 1 eu). The free energy of binding depends strongly up...


    pub_type: 杂志文章


    authors: Kresheck GC,Vitello LB,Erman JE

    更新日期:1995-07-04 00:00:00

  • Ivermectin and nodulisporic acid receptors in Drosophila melanogaster contain both gamma-aminobutyric acid-gated Rdl and glutamate-gated GluCl alpha chloride channel subunits.

    abstract::35S-labeled derivatives of the insecticides nodulisporic acid and ivermectin were synthesized and demonstrated to bind with high affinity to a population of receptors in Drosophila head membranes that were previously shown to be associated with a glutamate-gated chloride channel. Nodulisporic acid binding was modeled ...


    pub_type: 杂志文章


    authors: Ludmerer SW,Warren VA,Williams BS,Zheng Y,Hunt DC,Ayer MB,Wallace MA,Chaudhary AG,Egan MA,Meinke PT,Dean DC,Garcia ML,Cully DF,Smith MM

    更新日期:2002-05-21 00:00:00

  • Adriamycin and daunorubicin bind in a cooperative manner to deoxyribonucleic acid.

    abstract::Phase partition techniques have been used to measure the binding of the antitumor drugs adriamycin (NSC-123127) and daunorubicin (NSC-82151) to various DNAs. These methods provide reliable equilibrium binding data at the low levels of drug binding that may be expected in vivo. Both adriamycin and daunorubicin exhibit ...


    pub_type: 杂志文章


    authors: Graves DE,Krugh TR

    更新日期:1983-08-02 00:00:00

  • Dissociation of apolipoprotein E oligomers to monomer is required for high-affinity binding to phospholipid vesicles.

    abstract::The apolipoprotein apoE plays a key role in cholesterol and lipid metabolism. There are three isoforms of this protein, one of which, apoE4, is the major risk factor for Alzheimer's disease. At micromolar concentrations all lipid-free apoE isoforms exist primarily as monomers, dimers, and tetramers. However, the molec...


    pub_type: 杂志文章


    authors: Garai K,Baban B,Frieden C

    更新日期:2011-04-05 00:00:00

  • Glutamate 329 located in the fourth transmembrane segment of the alpha-subunit of the rat kidney Na+,K+-ATPase is not an essential residue for active transport of sodium and potassium ions.

    abstract::An allelic variant of the ouabain-insensitive rat kidney Na+,K(+)-ATPase alpha 1-isoform was identified by chance in a cDNA library. The variant differed from the wild-type rat kidney Na+,K(+)-ATPase by a single G to C base substitution in the cDNA, which on the amino acid level gave rise to a glutamine in place of th...


    pub_type: 杂志文章


    authors: Vilsen B

    更新日期:1993-12-07 00:00:00

  • Kinetic mechanism for the model reaction of NADPH-cytochrome P450 oxidoreductase with cytochrome c.

    abstract::The kinetic mechanism of NADPH-cytochrome P450 oxidoreductase (P450R) has been determined for the model reaction with cytochrome c3+. Although initial velocity studies show parallel patterns, consistent with a classical (one-site) ping-pong mechanism that precludes the formation of a ternary NADPH-P450R-cytochrome c3+...


    pub_type: 杂志文章


    authors: Sem DS,Kasper CB

    更新日期:1994-10-11 00:00:00

  • Epigallocatechin-3-gallate Inhibits Cu(II)-Induced β-2-Microglobulin Amyloid Formation by Binding to the Edge of Its β-Sheets.

    abstract::Epigallocatechin-3-gallate (EGCG) is a catechin found in green tea that can inhibit the amyloid formation of a wide variety of proteins. EGCG's ability to prevent or redirect the amyloid formation of so many proteins may reflect a common mechanism of action, and thus, greater molecular-level insight into how it exerts...


    pub_type: 杂志文章


    authors: Marcinko TM,Drews T,Liu T,Vachet RW

    更新日期:2020-03-17 00:00:00

  • Structure and mechanism of an ADP-glucose phosphorylase from Arabidopsis thaliana.

    abstract::The X-ray crystal structure of the At5g18200.1 protein has been determined to a nominal resolution of 2.30 A. The structure has a histidine triad (HIT)-like fold containing two distinct HIT-like motifs. The sequence of At5g18200.1 indicates a distant family relationship to the Escherichia coli galactose-1-P uridylyltr...


    pub_type: 杂志文章


    authors: McCoy JG,Arabshahi A,Bitto E,Bingman CA,Ruzicka FJ,Frey PA,Phillips GN Jr

    更新日期:2006-03-14 00:00:00

  • Enzymic and physicochemical properties of Streptomyces griseus trypsin.

    abstract::Streptomyces griseus trypsin has been isolated from Pronase by ion-exchange chromatography on CM-Sephadex and SE-Sephadex. The isolated enzyme was homogeneous by the criteria tested except for a low degree of contamination by an enzyme with nontryptic activity. The latter could be partially resolved by chromatography ...


    pub_type: 杂志文章


    authors: Olafson RW,Smillie LB

    更新日期:1975-03-25 00:00:00

  • Proton nuclear magnetic resonance investigation of cross-linked asymmetrically modified hemoglobins: influence of the salt bridges on tertiary and quaternary structures of hemoglobin.

    abstract::Asymmetrically modified hemoglobins, [alpha(des-Arg)beta]A[alpha beta]cXL, [alpha(des-Arg-Tyr)beta]A[alpha beta]cXL, [alpha(des-Arg)beta(NES)]A[alpha beta]cXL, and [alpha(des-Arg)beta]A[alpha beta(NES)]cXL, have been prepared from chemically modified human normal adult hemoglobin (Hb A) and mutant hemoglobin C (beta 6...


    pub_type: 杂志文章


    authors: Miura S,Ho C

    更新日期:1984-05-22 00:00:00

  • Synthesis and properties of fluorescent beta-adrenoceptor ligands.

    abstract::We describe the synthesis of bordifluoropyrromethene (BODIPY), fluorescein, and related fluorescent derivatives of the beta-adrenergic ligand CGP 12177. With these probes we screened insect (Sf9) cells stably transformed with the human beta 2-adrenoceptor gene and expressing (2-3.5) x 10(5) human beta 2-adrenoceptors ...


    pub_type: 杂志文章


    authors: Heithier H,Hallmann D,Boege F,Reiländer H,Dees C,Jaeggi KA,Arndt-Jovin D,Jovin TM,Helmreich EJ

    更新日期:1994-08-09 00:00:00