Effect of basic compounds on the polymerization of clathrin.

abstract：:The activity of phospholipase A2 from snake venom to hydrolyze bilayers of phosphatidylcholines is greatly enhanced by the presence of the hydrolysis products, lysolecithin and fatty acid, in the bilayer. The fluorescence of several probes of membrane structure was used to monitor changes in bilayer physical propertie...

journal_title：Biochemistry

authors： Sheffield MJ,Baker BL,Li D,Owen NL,Baker ML,Bell JD

更新日期：1995-06-20 00:00:00

abstract：:Most A/T specific heterocyclic diamidine derivatives need at least four A/T base pairs for tight binding to the DNA minor groove. Addition of a GC base pair to A/T sequences typically causes a large decrease in binding constant. The ability to target biologically important sequences of DNA could be significantly incre...

journal_title：Biochemistry

authors： Rahimian M,Kumar A,Say M,Bakunov SA,Boykin DW,Tidwell RR,Wilson WD

更新日期：2009-02-24 00:00:00

abstract：:Iron K-edge X-ray spectroscopy (XANES and EXAFS) was used to study iron coordination in frozen solutions of soybean lipoxygenase-1 (SLO). The intensity of the 1s-->3d pre-edge transition of native iron(II) lipoxygenase is greater than what was found for six-coordinate high-spin iron(II) model complexes, but comparable...

journal_title：Biochemistry

authors： Scarrow RC,Trimitsis MG,Buck CP,Grove GN,Cowling RA,Nelson MJ

更新日期：1994-12-20 00:00:00

abstract：:GlgE (EC 2.4.99.16) is an α-maltose 1-phosphate:(1→4)-α-d-glucan 4-α-d-maltosyltransferase of the CAZy glycoside hydrolase 13_3 family. It is the defining enzyme of a bacterial α-glucan biosynthetic pathway and is a genetically validated anti-tuberculosis target. It catalyzes the α-retaining transfer of maltosyl units...

journal_title：Biochemistry

authors： Syson K,Stevenson CE,Rashid AM,Saalbach G,Tang M,Tuukkanen A,Svergun DI,Withers SG,Lawson DM,Bornemann S

更新日期：2014-04-22 00:00:00

abstract：:Myosins are a superfamily of actin-binding motor proteins with significant variations in kinetic properties (such as actin binding affinity) between different isoforms. It remains unknown how such kinetic variations arise from the structural and dynamic tuning of the actin-myosin interface at the amino acid residue le...

journal_title：Biochemistry

authors： Li M,Zheng W

更新日期：2013-11-26 00:00:00

abstract：:An antibody population that reacted with the high-affinity lysine binding site of human plasminogen was elicited by immunizing rabbits with an elastase degradation product containing kringles 1-3 (EDP I). This antibody was immunopurified by affinity chromatography on plasminogen-Sepharose and elution with 0.2 M 6-amin...

journal_title：Biochemistry

authors： Miles LA,Plow EF

更新日期：1986-11-04 00:00:00

abstract：:Various omega-halogenated carboxy acids and amides were evaluated as potential active-site-directed reagents for alcohol dehydrogenase. 2-Bromoacetamide and bromoacetic and 3-bromopropionic acids inactivated the enzyme; AMP, NAD+, and NADH markedly decreased the rate of inactivation. Some omega-halogenated carboxyamid...

journal_title：Biochemistry

authors： Fries RW,Bohlken DP,Blakley RT,Plapp BV

更新日期：1975-11-18 00:00:00

abstract：:The di-heme enzyme MauG catalyzes the oxidative biosynthesis of a tryptophan tryptophylquinone cofactor on a precursor of the enzyme methylamine dehydrogenase (preMADH). Reaction of H2O2 with the diferric form of MauG, or reaction of O2 with diferrous MauG, forms the catalytic intermediate known as bis-Fe(IV), which a...

journal_title：Biochemistry

authors： Yukl ET,Williamson HR,Higgins L,Davidson VL,Wilmot CM

更新日期：2013-12-31 00:00:00

abstract：:The substrate specificity of the human enzyme glutathione reductase was changed from its natural substrate glutathione to trypanothione [N1,N8-bis(glutathionyl)spermidine] by site-directed mutagenesis of two residues. The glutathione analogue, trypanothione, is the natural substrate for trypanothione reductase, an enz...

journal_title：Biochemistry

authors： Bradley M,Bücheler US,Walsh CT

更新日期：1991-06-25 00:00:00

abstract：:Penicillanic acid sulfone (1) is both a substrate and an inactivator of the RTEM beta-lactamase. About 7000 hydrolytic events occur before enzyme inactivation. The 6,6-dideuterio sulfone shows a 3-fold acceleration of both the hydrolysis reaction and the enzyme inactivation. The kinetic and spectroscopic results are n...

journal_title：Biochemistry

authors： Brenner DG,Knowles JR

更新日期：1981-06-23 00:00:00

abstract：:A 15-residue fragment within the major continuous domain of gp120 from HIV-1 that can bind independently to the CD4 receptor has been shown to have the property of behaving as a solvent polarity-dependent conformational switch. The switch behavior (cooperative transition from beta-sheet to helical conformation as a fu...

journal_title：Biochemistry

authors： Graf von Stosch A,Kinzel V,Reed J

更新日期：1996-01-16 00:00:00

abstract：:The effect of mutating Gly 478 to Ala in rat cytochrome P450 2B1 on the metabolism of N-benzyl-1-aminobenzotriazole was investigated. The 7-ethoxy-4-(trifluoromethyl)coumarin O-deethylation activity of the wild-type enzyme was completely inactivated by incubating with 1 microM BBT. The G478A mutant, however, was not i...

journal_title：Biochemistry

authors： Kent UM,Hanna IH,Szklarz GD,Vaz AD,Halpert JR,Bend JR,Hollenberg PF

更新日期：1997-09-30 00:00:00

abstract：:Using the baculovirus/Sf9 cell expression system, we have incorporated 99% 15N-enriched [alpha,epsilon-15N2]-L-lysine into the rod visual pigment rhodopsin. We have subsequently investigated the protonated Schiff base (pSB) linkage in the [alpha, epsilon-15N2]Lys-rhodopsin with cross-polarization magic angle spinning ...

journal_title：Biochemistry

authors： Creemers AF,Klaassen CH,Bovee-Geurts PH,Kelle R,Kragl U,Raap J,de Grip WJ,Lugtenburg J,de Groot HJ

更新日期：1999-06-01 00:00:00

abstract：:The late intermediates involved in the activation mechanism of bovine rhodopsin are investigated by time-resolved optical absorption spectroscopy. Measurements from 10 μs to 200 ms after photolysis were carried out on membrane suspensions of bovine rhodopsin at a temperature of 15 °C and at pH of 7.3, 8.0, and 8.7. Th...

journal_title：Biochemistry

authors： Szundi I,Funatogawa C,Kliger DS

更新日期：2016-09-13 00:00:00

abstract：:The interaction of the 20-kDa pore-forming domain of colicin A with phospholipid vesicles was investigated by gel permeation chromatography, analytical centrifugation, and electron microscopy. Under the experimental conditions of this study, this peptide was found to interact only with vesicles containing negatively c...

journal_title：Biochemistry

authors： Massotte D,Dasseux JL,Sauve P,Cyrklaff M,Leonard K,Pattus F

更新日期：1989-09-19 00:00:00

abstract：:The X-ray crystal structure of human soluble epoxide hydrolase (sEH) has been determined at 2.6 A resolution, revealing a domain-swapped quaternary structure identical to that observed for the murine enzyme [Argiriadi, M. A., Morisseau, C., Hammock, B. D., and Christianson, D. W. (1999) Proc. Natl. Acad. Sci. U.S.A. 9...

journal_title：Biochemistry

authors： Gomez GA,Morisseau C,Hammock BD,Christianson DW

更新日期：2004-04-27 00:00:00

abstract：:Bovine milk galactosyltransferase was examined by ESR and NMR proton relaxation measurements to determine the stoichiometry and nature of manganese and UDP-Gal substrate binding. The ESR and NMR data clearly showed the binding of two (Mn(II) per mol of enzyme in the ternary complex (enzyme-manganese-UDP-Gal). The affi...

journal_title：Biochemistry

authors： Andree PJ,Berliner LJ

更新日期：1980-03-04 00:00:00

abstract：:To elucidate early stages in protein folding, we have adopted a fragment reconstitution method for small proteins. This approach is expected to provide nuclei for protein folding and to allow us to investigate folding mechanisms. In previous work [Kobayashi, N., et al. (1995) FEBS Lett. 366, 99-103.] we demonstrated t...

journal_title：Biochemistry

authors： Honda S,Kobayashi N,Munekata E,Uedaira H

更新日期：1999-01-26 00:00:00

abstract：:Quasi-elastic laser light scattering is used to examine the cholera toxin mediated agglutination of ganglioside GM1 containing phospholipid vesicles and Gm1-coated polystyrene spheres. We find that the ability of cholera toxin to agglutinate Gm1-containing phospholipid vesicles depends markedly on the lipid compositio...

journal_title：Biochemistry

authors： Dwyer JD,Bloomfield VA

更新日期：1982-06-22 00:00:00

abstract：:We have investigated the topology of the band 3 protein of the human erythrocyte membrane by a combination of chemical labeling and proteolytic cleavage. The N-terminal third of the membrane-bound domain of band 3 is a 17 000-dalton chymotryptic fragment that is known to traverse the membrane an odd number of times. A...

journal_title：Biochemistry

authors： Jennings ML,Nicknish JS

更新日期：1984-12-18 00:00:00

abstract：:Internal loops in RNA are important for folding and function. Consecutive noncanonical pairs can form in internal loops having at least two nucleotides on each side. Thermodynamic and structural insights into such internal loops should improve approximations for their stabilities and predictions of secondary and three...

journal_title：Biochemistry

authors： Chen G,Turner DH

更新日期：2006-03-28 00:00:00

abstract：:The amino acid composition and sequence of EIIMtl is known [Lee, C. A., & Saier, M. H., Jr. (1983) J. Biol. Chem. 258, 10761-10767]. This information was combined, in the present study, with quantitative amino acid analysis to determine the molar concentration of the enzyme. The stoichiometry of phosphoryl group incor...

journal_title：Biochemistry

authors： Pas HH,ten Hoeve-Duurkens RH,Robillard GT

更新日期：1988-07-26 00:00:00

abstract：:Endogenous protein kinase inhibitors are essential for a wide range of physiological functions. These endogenous inhibitors may mimic peptide substrates as in the case of the heat-stable protein kinase inhibitor (PKI), or they may mimic nucleotide triphosphates. Natural product inhibitors, endogenous to the unique org...

journal_title：Biochemistry

authors： Narayana N,Diller TC,Koide K,Bunnage ME,Nicolaou KC,Brunton LL,Xuong NH,Ten Eyck LF,Taylor SS

更新日期：1999-02-23 00:00:00

abstract：:Human neutrophil cathepsin G or bovine chymotrypsin proteolytically cleaved human alpha-thrombin at the B-chain Trp148-Thr149 bond generating a new form, zeta-thrombin. While incubation of alpha-thrombin with cathepsin G at pH 7.4 and 37 degrees C resulted in a partial loss of fibrinogen clotting activity, 86 +/- 13% ...

journal_title：Biochemistry

authors： Brezniak DV,Brower MS,Witting JI,Walz DA,Fenton JW 2nd

更新日期：1990-04-10 00:00:00

abstract：:ncd is a kinesin-related motor protein from Drosophila that moves in the opposite direction along microtubules to kinesin. To learn more about the ncd mechanism, ncd motor domain (R335-K700) was expressed in Escherichia coli and its enzymatic characteristics were studied. The ncd motor domain was purified from the cel...

journal_title：Biochemistry

authors： Shimizu T,Sablin E,Vale RD,Fletterick R,Pechatnikova E,Taylor EW

更新日期：1995-10-10 00:00:00

abstract：:The effects of solution variables on the in vitro reconstitution of calf brain tubulin, purified by the method of Weisenberg et al. (Weisenberg, R. C., Borisy, G. G., and Taylor, E. W. (1968), Biochemistry 7, 4466-4479; Weisenberg, R. C., and Timasheff, S. N. (1970), Biochemistry 9, 4110-4116), as modified by Lee et a...

journal_title：Biochemistry

authors： Lee JC,Timasheff SN

更新日期：1977-04-19 00:00:00

abstract：:Through a ligand-directed tosyl (LDT) chemistry strategy using the synthetic acetogenin ligand AL1, we succeeded in the pinpoint alkynylation (-C≡CH) of Asp160 in the 49 kDa subunit of bovine complex I, which may be located in the inner part of the putative quinone binding cavity of the enzyme [Masuya, T., et al. (201...

journal_title：Biochemistry

authors： Masuya T,Murai M,Morisaka H,Miyoshi H

更新日期：2014-12-16 00:00:00

abstract：:The catalytic and structural properties of divalent metal ion cofactor binding sites in the dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE) from Haemophilus influenzae were investigated. Co(II)-substituted DapE enzyme was 25% more active than the Zn(II)-loaded form of the enzyme. Interestingly, Mn...

journal_title：Biochemistry

authors： Bienvenue DL,Gilner DM,Davis RS,Bennett B,Holz RC

更新日期：2003-09-16 00:00:00

abstract：:Maduropeptin (MDP) is a recently isolated antitumor antibiotic, consisting of an enediyne-containing chromophore embedded in a highly acidic protein. This holoantibiotic damages duplex DNA in vitro, producing a mixture of single- and double-strand breaks at selected sites. The chromophore, isolated as the methanol add...

journal_title：Biochemistry

authors： Zein N,Solomon W,Colson KL,Schroeder DR

更新日期：1995-09-12 00:00:00

abstract：:Cytochrome b5 reductase (cb5r), a member of the ferredoxin:NADP+ reductase family of flavoprotein transhydrogenases, catalyzes the NADH-dependent reduction of cytochrome b5. Within this family, a conserved "GxGxxP" sequence motif has been implicated in binding reduced pyridine nucleotides. However, Glycine 179, a cons...

journal_title：Biochemistry

authors： Roma GW,Crowley LJ,Davis CA,Barber MJ

更新日期：2005-10-18 00:00:00