Penicillanic acid sulfone: an unexpected isotope effect in the interaction of 6 alpha- and 6 beta-monodeuterio and of 6,6-dideuterio derivatives with RTEM beta-lactamase from Escherichia coli.

abstract：:Two site-directed mutant enzymes of the class A beta-lactamase from Staphylococcus aureus PC1 were produced with the goal of blocking the site that in the native enzyme is occupied by the proposed hydrolytic water molecule. The crystal structures of these two mutant enzymes, N170Q and N170M, have been determined and r...

journal_title：Biochemistry

authors： Zawadzke LE,Chen CC,Banerjee S,Li Z,Wäsch S,Kapadia G,Moult J,Herzberg O

更新日期：1996-12-24 00:00:00

abstract：:The membrane receptor for immunoglobulin E (IgE) and its ligand, IgE, were irradiated with high-energy electrons. Loss of binding activity was measured for each, and the size of the functional targets was assessed. In both cases, the target size was substantially smaller than the covalent structure of the molecule. Th...

journal_title：Biochemistry

authors： Fewtrell C,Kempner E,Poy G,Metzger H

更新日期：1981-11-10 00:00:00

abstract：:HbPresbyterian (beta 108Asn --> Lys, HbP) contains an additional positive charge (per alpha beta dimer) in the middle of the central cavity and exhibits a lower oxygen affinity than wild-type HbA in the presence of chloride. However, very little is known about the molecular origins of its altered functional properties...

journal_title：Biochemistry

authors： Gottfried DS,Manjula BN,Malavalli A,Acharya AS,Friedman JM

更新日期：1999-08-31 00:00:00

abstract：:High-resolution 2D NMR spectra of the duplex CGCGAATTCGCG with deoxyribose sugars but with the normal phosphodiester linker replaced by an N3' --> P5' phosphoramidate (NP) group have been used to establish a solution structure for the duplex. Distance, angle, and base pair hydrogen-bonding constraints were used to ref...

journal_title：Biochemistry

authors： Ding D,Gryaznov SM,Wilson WD

更新日期：1998-09-01 00:00:00

abstract：:Myocardial contraction is initiated when Ca2+ binds to site II of cardiac troponin C. This 12-residue EF-hand loop (NH2-DEDGSGTVDFDE-COOH) contains six residues (bold) that coordinate Ca2+ binding and six residues that do not appear to influence Ca2+ binding directly. We have introduced six single-cysteine substitutio...

journal_title：Biochemistry

authors： Reece KL,Moss RL

更新日期：2008-05-06 00:00:00

abstract：:The complete amino acid sequence of the 4Fe-4S ferredoxin from the thermophilic bacterium Clostridium thermoaceticum has been determined. The protein is extremely thermostable and is the only known clostridial ferredoxin to contain a single [4Fe-4S] cluster. The sequence totals 63 residues and includes the first trypt...

journal_title：Biochemistry

authors： Elliott JI,Yang SS,Ljungdahl LG,Travis J,Reilly CF

更新日期：1982-07-06 00:00:00

abstract：:Proton and phosphorus-31 nuclear magnetic resonance (1H and 31P NMR) studies of the interaction between a tridecapeptide pheromone, the alpha-factor of Saccharomyces cerevisiae, and sonicated lipid vesicles are reported. 31P NMR studies demonstrate that there is interaction of the peptide with the phosphorus headgroup...

journal_title：Biochemistry

authors： Jelicks LA,Broido MS,Becker JM,Naider FR

更新日期：1989-05-16 00:00:00

abstract：:The binding of a ligand to cell surface receptors initiates a cascade of intracellular signals that generate responses to the external stimuli. Thus, this event plays a pivotal role in the mechanism of transmembrane signaling. Activin is a member of a cytokine family that is involved in diverse biological processes. T...

journal_title：Biochemistry

authors： Greenwald J,Le V,Corrigan A,Fischer W,Komives E,Vale W,Choe S

更新日期：1998-11-24 00:00:00

abstract：:The UV endonucleases [endodeoxyribonuclease (pyrimidine dimer), EC 3.1.25.1] from Micrococcus luteus and bacteriophage T4 possess two catalytic activities specific for the site of cyclobutane pyrimidine dimers in UV-irradiated DNA: a DNA glycosylase that cleaves the 5'-glycosyl bond of the dimerized pyrimidines and an...

journal_title：Biochemistry

authors： Liuzzi M,Weinfeld M,Paterson MC

更新日期：1987-06-16 00:00:00

abstract：:Hemocyanin (Hc) is a dinuclear copper protein that binds oxygen reversibly. The structure of the Cu(II) site in a derivative of hemocyanin known as green half-met (GHM) has been analyzed using the pulsed EPR technique of electron spin-echo envelope modulation (ESEEM) spectroscopy. The derivative, prepared by treating ...

journal_title：Biochemistry

authors： Magliozzo RS,Bubacco L,McCracken J,Jiang F,Beltramini M,Salvato B,Peisach J

更新日期：1995-02-07 00:00:00

abstract：:The committed step in menaquinone biosynthesis is the formation of o-succinylbenzoate (OSB). It is presumed to require the reaction of a seven-carbon intermediate of the shikimate pathway with a succinic semialdehyde-thiamin pyrophosphate (TPP) anion, derived by decarboxylation of 2-ketoglutarate. The following eviden...

journal_title：Biochemistry

authors： Marley MG,Meganathan R,Bentley R

更新日期：1986-03-25 00:00:00

abstract：:A member of the SF2 family of helicases, Escherichia coli RecQ, is involved in the recombination and repair of double-stranded DNA breaks and single-stranded DNA (ssDNA) gaps. Although the unwinding activity of this helicase has been studied biochemically, the mechanism of translocation remains unclear. To this end, u...

journal_title：Biochemistry

authors： Rad B,Kowalczykowski SC

更新日期：2012-04-03 00:00:00

abstract：:Glycosylases specifically recognize and flip their target base out of the DNA helix into the enzyme's active site. Our simulations show that a partially flipped state, already present in free DNA carrying a T:G mispair, becomes the more probable state compared to the closed state after binding of thymine DNA glycosyla...

journal_title：Biochemistry

authors： Kanaan N,Imhof P

更新日期：2018-10-02 00:00:00

abstract：:The crystal structure of netropsin bound to the decamer d(CGCAATTGCG) has been determined at 2.4 A resolution. This is the first example of a crystal structure of netropsin bound to decamer DNA. The central eight bases of each DNA single-strand base pair with a self-complementary strand to form an octamer B-DNA duplex...

journal_title：Biochemistry

authors： Nunn CM,Garman E,Neidle S

更新日期：1997-04-22 00:00:00

abstract：:1H NMR spectral editing techniques can select the distinct signals of lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate and provide a unique way to monitor the biochemical processes in vivo. These metabolite levels reflect the near-equilibrium dehydrogenase activity and therefore the cellular redox state. The ...

journal_title：Biochemistry

authors： Chung Y,Jue T

更新日期：1992-11-17 00:00:00

abstract：:NLRP4 is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family of cytosolic receptors and a member of an inflammation signaling cascade. Here, we present the crystal structure of the NLRP4 pyrin domain (PYD) at 2.3 Å resolution. The NLRP4 PYD is a member of the death domain (DD) superfamily ...

journal_title：Biochemistry

authors： Eibl C,Grigoriu S,Hessenberger M,Wenger J,Puehringer S,Pinheiro AS,Wagner RN,Proell M,Reed JC,Page R,Diederichs K,Peti W

更新日期：2012-09-18 00:00:00

abstract：:We have used flash-detection optical and electron paramagnetic resonance spectroscopy to measure the kinetics and yield per flash of the photooxidation of cytochrome b559 and the yield per flash of the photooxidation of the tyrosine residue YD in Mn-depleted photosystem II (PSII) membranes at room temperature. The ini...

journal_title：Biochemistry

authors： Buser CA,Thompson LK,Diner BA,Brudvig GW

更新日期：1990-09-25 00:00:00

abstract：:cAMP binding of the androgen receptor (AR) from murine skeletal muscle was studied. Testosterone affinity chromatography yielded androgen receptor with about 4000-fold purification. Determination of the cAMP binding in the affinity eluate, by adsorption of protein-cAMP complexes to cellulose ester filters or removal o...

journal_title：Biochemistry

authors： Ofenloch-Hähnle B,Eisele K

更新日期：1988-11-15 00:00:00

abstract：:A covalent complex between tRNA (m5U54)methyltransferase, 5-fluorouridine tRNA(Phe), and S-adenosyl-L-[methyl-3H]methionine was formed in vitro and purified. Previously, it was shown that in this complex the 6-position of fluorouridine-54 is covalently linked to a catalytic nucleophile and the 5-position is bound to t...

journal_title：Biochemistry

authors： Kealey JT,Santi DV

更新日期：1991-10-08 00:00:00

abstract：:Specific lysine residues on the ubiquitin surface were selected during the course of evolution to form different polyubiquitin chain structures that signal diverse cellular processes. A vast number of ubiquitin receptors specifically recognize and decode the signals conferred by these polyubiquitin chains. The mechani...

journal_title：Biochemistry

authors： Levin-Kravets O,Shohat N,Prag G

更新日期：2015-08-04 00:00:00

abstract：:Yah1p, an [Fe 2S 2]-containing ferredoxin located in the matrix of Saccharomyces cerevisiae mitochondria, functions in the synthesis of Fe/S clusters and heme a prosthetic groups. EPR, Mossbauer spectroscopy, and electron microscopy were used to characterize the Fe that accumulates in Yah1p-depleted isolated intact mi...

journal_title：Biochemistry

authors： Miao R,Martinho M,Morales JG,Kim H,Ellis EA,Lill R,Hendrich MP,Münck E,Lindahl PA

更新日期：2008-09-16 00:00:00

abstract：:The interactions of Alzheimer's amyloid beta-peptide with cyclodextrins were studied by (1)H NMR: the translational diffusion coefficient of the peptide and chemical shift changes were studied by the presence of variable concentrations of cyclodextrins. For the full-length peptide, Abeta(1-40), the combined results of...

journal_title：Biochemistry

authors： Danielsson J,Jarvet J,Damberg P,Gräslund A

更新日期：2004-05-25 00:00:00

abstract：:Nuclear Overhauser effect (NOE) measurements are reported for the nonexchangeable base and ribose 1' proton resonances of CCGGAp, ACCGGp, and ACCGGUp. The results permit assignment of these resonances to particular nucleotides in the sequences. The NOE data rule out conformations containing syn glycosidic linkages and...

journal_title：Biochemistry

authors： Petersheim M,Turner DH

更新日期：1983-01-18 00:00:00

abstract：:The interaction of solvent of the substrate binding domain of the bacterial heat shock 70 chaperone protein DnaK was studied in its apo form and with bound hydrophobic substrate peptide, using refined nuclear magnetic resonance experiments. Distinct differences between the two states of the protein were observed. Acco...

journal_title：Biochemistry

authors： Cai S,Stevens SY,Budor AP,Zuiderweg ER

更新日期：2003-09-30 00:00:00

abstract：:The enzyme hydrogenase, from the photosynthetic bacterium Chromatium, was purified to homogeneity after solubilization of the particulate enzyme with deoxycholate. The purification procedure included ammonium sulfate fractionation, treatment with manganous phosphate gel, heating at 63 degrees, DEAE-cellulose chromatog...

journal_title：Biochemistry

authors： Gitlitz PH,Krasna AI

更新日期：1975-06-17 00:00:00

abstract：:The Plasmodium falciparum malarial parasite genome appears to encode one and only one phosphatidylinositol 3'-kinase (PI3K), and sequence analysis suggests that the enzyme is a "class III"- or "Vps34"-type PI3K. PfVps34 has generated excitement as a possible druggable target and potentially a key target of artemisinin...

journal_title：Biochemistry

authors： Hassett MR,Sternberg AR,Riegel BE,Thomas CJ,Roepe PD

更新日期：2017-08-22 00:00:00

abstract：:Trimethylamine dehydrogenase (TMADH) from the bacterium Methylophilus methylotrophus (sp. W(3)A(1)) and its C30A mutant were inactivated with three known inactivators of monoamine oxidase, namely, phenylhydrazine, N-cyclopropyl-alpha-methylbenzylamine, and 1-phenylcyclopropylamine. All three compounds irreversibly ina...

journal_title：Biochemistry

authors： Mitchell DJ,Nikolic D,Jang MH,van Breemen RB,Hille R,Silverman RB

更新日期：2001-07-24 00:00:00

abstract：:Recent evidence adds support to a traditional concept according to which the eukaryotic nucleus is organized into functional domains by scaffold or matrix attachment regions (S/MARs). These regions have previously been predicted to have a high potential for stress-induced duplex destabilization (SIDD). Here we report ...

journal_title：Biochemistry

authors： Goetze S,Gluch A,Benham C,Bode J

更新日期：2003-01-14 00:00:00

abstract：:Resonance Raman and UV-visible absorption spectra show that nickel(II) microperoxidase-11 (NiMP-11) is four-coordinate in aqueous solution in the pH range from 1.0 to 13.0. In aqueous solutions of NiMP-11 in the absence of cetyltrimethylammonium bromide (CTAB), NiMP-11 is aggregated. In CTAB micellar solutions, where ...

journal_title：Biochemistry

authors： Ma JG,Vanderkooi JM,Zhang J,Jia SL,Shelnutt JA

更新日期：1999-03-02 00:00:00

abstract：:Rh(phen)2phi3+ (phen = 1,10-phenanthroline; phi = 9,10-phenanthrenequinone diimine) avidly binds to DNA via intercalation from the major groove and upon photoactivation produces strand scission with single base 5' asymmetry. Enantiomers of Rh(phen)2phi3+, which lack hydrogen-bonding substituents in ancillary positions...

journal_title：Biochemistry

authors： Campisi D,Morii T,Barton JK

更新日期：1994-04-12 00:00:00