Metal ion and substrate binding to bovine galactosyltransferase.

abstract：:The dynamics of the single-stranded, helix--coil transition of poly(adenylic acid) have been investigated by using the Raman laser temperature jump technique. The driving forces of this conformational transition have been probed by varying the cosolvent in mixed aqueous solutions. The rate of helix formation correlate...

journal_title：Biochemistry

authors： Dewey TG,Turner DH

更新日期：1980-04-15 00:00:00

abstract：:The interactions of Alzheimer's amyloid beta-peptide with cyclodextrins were studied by (1)H NMR: the translational diffusion coefficient of the peptide and chemical shift changes were studied by the presence of variable concentrations of cyclodextrins. For the full-length peptide, Abeta(1-40), the combined results of...

journal_title：Biochemistry

authors： Danielsson J,Jarvet J,Damberg P,Gräslund A

更新日期：2004-05-25 00:00:00

abstract：:Chicken nucleoside triphosphate diphosphohydrolase 8 (NTPDase8) is a cell surface ectonucleotidase with a large extracellular domain (ECD) containing the active site and is anchored to the membrane by two transmembrane domains (TMDs) at the N- and C-termini. Unlike other cell surface NTPDases that have been characteri...

journal_title：Biochemistry

authors： Li CS,Lee Y,Knowles AF

更新日期：2010-01-12 00:00:00

abstract：:Escherichia coli asparagine synthetase B (AS-B) catalyzes the synthesis of asparagine from aspartate, glutamine, and ATP. A combination of kinetic, isotopic-labeling, and stoichiometry studies have been performed to define the nature of nitrogen transfer mediated by AS-B. The results of initial rate studies were consi...

journal_title：Biochemistry

authors： Boehlein SK,Stewart JD,Walworth ES,Thirumoorthy R,Richards NG,Schuster SM

更新日期：1998-09-22 00:00:00

abstract：:Manganese peroxidase (MnP) from Phanerochaete chrysosporium undergoes a pH-dependent conformational change evidenced by changes in the electronic absorption spectrum. This high- to low-spin alkaline transition occurs at approximately 2 pH units lower in an F190I mutant MnP when compared to the wild-type enzyme. Herein...

journal_title：Biochemistry

authors： Youngs HL,Moënne-Loccoz P,Loehr TM,Gold MH

更新日期：2000-08-15 00:00:00

abstract：:We have recently reported that the Na pump alpha-subunit has cytosolic-oriented oligosaccharides which were sensitive to cleavage by an enzyme specific for hydrolysis of N-linked glycans [Pedemonte et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 9789-9793]. We now describe experiments that characterize the saccharides...

journal_title：Biochemistry

authors： Pedemonte CH,Kaplan JH

更新日期：1992-11-03 00:00:00

abstract：:Subunit a of the Escherichia coli ATP synthase is thought to control access of protons to the ring of c subunits during proton-driven ATP synthesis. In this study, the surface exposure of subunit a in the periplasm has been examined using 3-N-maleimidyl-propionyl biocytin labeling in cells permeabilized by polymyxin B...

journal_title：Biochemistry

authors： Zhang D,Vik SB

更新日期：2003-01-21 00:00:00

abstract：:RNA binding proteins play an important role in regulating alternative pre-mRNA splicing and in turn cellular gene expression. Many of these RNA binding proteins occur as gene families with members sharing a high degree of primary structure identity and domain organization yet have tissue-specific expression patterns a...

journal_title：Biochemistry

authors： Pina JM,Reynaga JM,Truong AAM,Keppetipola NM

更新日期：2018-07-03 00:00:00

abstract：:Elongation factor Tu (EF-Tu) is a highly conserved GTPase that is responsible for supplying the aminoacylated tRNA to the ribosome. Upon binding to the ribosome, EF-Tu undergoes GTP hydrolysis, which drives a major conformational change, triggering the release of aminoacylated tRNA to the ribosome. Using a combination...

journal_title：Biochemistry

authors： Lai J,Ghaemi Z,Luthey-Schulten Z

更新日期：2017-11-14 00:00:00

abstract：:The E-domain of staphylococcal protein A is one of five homologous IgG-binding domains designated E, D, A, B, and C that comprise the extracellular portion of protein A. The E-domain binds tightly to Fc fragments of IgG and binds certain Fv fragments with micromolar affinity. To explore further the structural features...

journal_title：Biochemistry

authors： Starovasnik MA,Skelton NJ,O'Connell MP,Kelley RF,Reilly D,Fairbrother WJ

更新日期：1996-12-03 00:00:00

abstract：:The Fpg protein from Escherichia coli is a multifunctional protein that excises damaged purine bases from DNA to generate aldehydic abasic sites and then catalyzes the successive cleavage of the phosphodiester bonds first on the 3'-side and then on the 5'-side of the abasic site to generate 5'- and 3'-phosphate ends, ...

journal_title：Biochemistry

authors： Bhagwat M,Gerlt JA

更新日期：1996-01-16 00:00:00

abstract：:Escherichia coli tyrosine kinase (Etk) regulates the export of pathogenic capsular polysaccharide (CPS) by intermolecularly autophosphorylating its C-terminal tyrosine cluster. The kinase Etk, however, needs to be first activated by the intramolecular phosphorylation of a tyrosine residue, Y574, next to the active sit...

journal_title：Biochemistry

authors： Lu T,Tan H,Lee D,Chen G,Jia Z

更新日期：2009-08-25 00:00:00

abstract：:Human protein Z is a vitamin K-dependent plasma glycoprotein, deficiency of which leads to a mild bleeding tendency. Protein Z appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. In this study, to characterize the gene for protein Z, its organization and structure...

journal_title：Biochemistry

authors： Fujimaki K,Yamazaki T,Taniwaki M,Ichinose A

更新日期：1998-05-12 00:00:00

abstract：:The three-dimensional structure of d[CGTA'CG], where A' = [2-NH2-A], was determined to atomic (1.35 A) resolution by single isomorphous replacement. The d[CGTA'CG] hexamer crystallizes in space group P3221, and is not isomorphous with other DNA hexanucleotides. Despite completely different crystal packing, the essenti...

journal_title：Biochemistry

authors： Parkinson GN,Arvanitis GM,Lessinger L,Ginell SL,Jones R,Gaffney B,Berman HM

更新日期：1995-11-28 00:00:00

abstract：:Heparin accelerates inhibition of factor XIa (fXIa) by the serpins antithrombin (AT) and C1-inhibitor (C1-INH) by more than 2 orders of magnitude. The mechanism of the heparin-mediated acceleration of fXIa inhibition by these serpins is incompletely understood, as heparin appears to interact with both the catalytic an...

journal_title：Biochemistry

authors： Yang L,Sun MF,Gailani D,Rezaie AR

更新日期：2009-02-24 00:00:00

abstract：:We measure the effects of low concentrations of helix-stabilizing cosolvents, including 2,2,2-trifluoroethanol (TFE), on the thermodynamics and kinetics of folding of the dimeric alpha-helical coiled coil derived from the leucine zipper region of bZIP transcriptional activator GCN4. The change in kinetic behavior upon...

journal_title：Biochemistry

authors： Kentsis A,Sosnick TR

更新日期：1998-10-13 00:00:00

abstract：:Muscarinic cholinergic receptors can appear to be more numerous when labeled by [(3)H]quinuclidinylbenzilate (QNB) than by N-[(3)H]methylscopolamine (NMS). The nature of the implied heterogeneity has been studied with M(2) receptors in detergent-solubilized extracts of porcine atria. The relative capacity for [(3)H]NM...

journal_title：Biochemistry

authors： Park PS,Sum CS,Pawagi AB,Wells JW

更新日期：2002-04-30 00:00:00

abstract：:The origin of the "S3" EPR signal from calcium-depleted photosystem 2 samples has been investigated. This signal is observed after freezing samples under illumination and has been assigned to an interaction between the manganese cluster and an oxidized histidine radical [Boussac et al. (1990) Nature 347; 303-306]. In ...

journal_title：Biochemistry

authors： Hallahan BJ,Nugent JH,Warden JT,Evans MC

更新日期：1992-05-19 00:00:00

abstract：:In vitro transcription assays have been used to study the rate of ribonucleic acid (RNA) synthesis from the Escherichia coli lactose promotor mutant lacL8UV5 contained on a 203-bp (base pair) restriction fragment. The half-life of long (63-base) RNA production from heparin-resistant RNA polymerase-promotor complexes w...

journal_title：Biochemistry

authors： Munson LM,Reznikoff WS

更新日期：1981-04-14 00:00:00

abstract：:Protein disulfide isomerase (PDI) utilizes the active site sequence Cys-Gly-His-Cys (CGHC; E degrees ' = -180 mV) to effect thiol-disulfide interchange during oxidative protein folding. Here, the Cys-Gly-Cys-NH(2) (CGC) peptide is shown to have a disulfide reduction potential (E degrees ' = -167 mV) that is close to t...

journal_title：Biochemistry

authors： Woycechowsky KJ,Raines RT

更新日期：2003-05-13 00:00:00

abstract：:We have measured the base-pair lifetimes in oligodeoxynucleotides containing tracts of A.T base pairs using imino proton magnetic resonance. When the tract contains more than four consecutive A.T base pairs, possibly including a 5'-AT step but not a 5'-TA step, anomalously long lifetimes are observed. For example, the...

journal_title：Biochemistry

authors： Leroy JL,Charretier E,Kochoyan M,Guéron M

更新日期：1988-12-13 00:00:00

abstract：:Oligodeoxyribonucleotides cross-linked by reductively activated mitomycin C (MC) were prepared and purified for the first time. The cross-linked products were structurally characterized by nucleoside and MC-nucleoside adduct analysis. Optimal conditions were established for the cross-linking reaction, resulting in hig...

journal_title：Biochemistry

authors： Borowy-Borowski H,Lipman R,Chowdary D,Tomasz M

更新日期：1990-03-27 00:00:00

abstract：:Amyloid deposition of human islet amyloid polypeptide (hIAPP) in the islets of Langerhans is closely associated with the pathogenesis of type II diabetes mellitus. Despite substantial evidence linking amyloidogenic hIAPP to loss of β-cell mass and decreased pancreatic function, the molecular mechanism of hIAPP cytotox...

journal_title：Biochemistry

authors： Sasahara K,Morigaki K,Okazaki T,Hamada D

更新日期：2012-09-04 00:00:00

abstract：:Restrictocin is a small basic protein produced by the fungus Aspergillus restrictus. It potently inhibits protein synthesis in eukaryotic cells by specifically cleaving a single phosphodiester bond in 28S rRNA. A histidine residue at position 49 in restrictocin has been implicated in its active site. A mutant of restr...

journal_title：Biochemistry

authors： Nayak SK,Batra JK

更新日期：1997-11-04 00:00:00

abstract：:Catalase (hydroperoxidase) HPII of Escherichia coli is the largest catalase so far characterized, existing as a homotetramer of 84 kDa subunits. Each subunit has a core structure that closely resembles small subunit catalases, supplemented with an extended N-terminal sequence and compact flavodoxin-like C-terminal dom...

journal_title：Biochemistry

authors： Chelikani P,Donald LJ,Duckworth HW,Loewen PC

更新日期：2003-05-20 00:00:00

abstract：:The antimycotic drug clotrimazole inhibits the function of the gastric H,K-ATPase in a manner similar to that observed for the Na,K-ATPase. Because of the high hydrophobicity of the compound, the interaction between clotrimazole and the ion pump occurs at the membrane domain in the apolar core of the membrane. The enz...

journal_title：Biochemistry

authors： Witzke A,Lindner K,Munson K,Apell HJ

更新日期：2010-06-01 00:00:00

abstract：:The photoaffinity reagent 6-azido-L-tryptophan was synthesized by chemical methods. It binds reversibly in the dark to the alpha 2 beta 2 complex of tryptophan synthase of Escherichia coli and forms a quinonoid intermediate with enzyme-bound pyridoxal phosphate (lambda max = 476 nm). The absorbance of this chromophore...

journal_title：Biochemistry

authors： Miles EW,Phillips RS

更新日期：1985-08-13 00:00:00

abstract：:Papain-solubilized human class II (HLA-DR) antigens have been purified from cadaveric spleens by ion-exchange chromatography, gel chromatography, and immunosorbent purification. The isolated papain-solubilized antigens comprised two subunits with apparent molecular weights of 23 000 and 30 000, respectively. The circu...

journal_title：Biochemistry

authors： Wiman K,Claesson L,Rask L,Trägårdh L,Peterson PA

更新日期：1982-10-12 00:00:00

abstract：:Calmodulin (CaM), by mediating the stimulation of the activity of two adenylyl cyclases (ACs), plays a key role in integrating the cAMP and Ca(2+) signaling systems. These ACs, AC1 and AC8, by decoding discrete Ca(2+) signals can contribute to fine-tuning intracellular cAMP dynamics, particularly in neurons where they...

journal_title：Biochemistry

authors： Masada N,Schaks S,Jackson SE,Sinz A,Cooper DM

更新日期：2012-10-09 00:00:00

abstract：:Binding of mitochondrial cyclophilin (CyP) to the inner mitochondrial membrane is induced by treatment of mitochondria with thiol reagents or oxidative stress and correlates with a sensitization to [Ca2+] of the cyclosporin A-sensitive mitochondrial permeability transition pore (MTP) [Connern, C. P., & Halestrap, A. P...

journal_title：Biochemistry

authors： Connern CP,Halestrap AP

更新日期：1996-06-25 00:00:00