Interaction of the pore-forming domain of colicin A with phospholipid vesicles.

abstract：:The intense intrinsic fluorescence emissions from several clinically relevant camptothecin drugs have been exploited in order to determine (1) the structural basis of drug binding to lipid bilayers, (2) the lipid bilayer stability of each drug's alpha-hydroxylactone moiety, a pharmacophore which is essential for antit...

journal_title：Biochemistry

authors： Burke TG,Mishra AK,Wani MC,Wall ME

更新日期：1993-05-25 00:00:00

abstract：:It has previously been shown that 9S, untransformed progestin, estrogen, androgen, and glucocorticoid receptor complexes in rabbit uterine and liver cytosols contain a 59-kDa protein [Tai, P. K., Maeda, Y., Nakao, K., Wakim, N. G., Duhring, J. L., & Faber, L. E. (1986) Biochemistry 25, 5269-5275]. In this work we show...

journal_title：Biochemistry

authors： Sanchez ER,Faber LE,Henzel WJ,Pratt WB

更新日期：1990-05-29 00:00:00

abstract：:In cells, RNAs likely adopt numerous intermediate conformations prior to formation of functional RNA-protein complexes. We used single-nucleotide resolution selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) to probe the structure of Escherichia coli 16S rRNA in healthy growing bacteria. SHAPE-direct...

journal_title：Biochemistry

authors： McGinnis JL,Weeks KM

更新日期：2014-05-20 00:00:00

abstract：:Neutral oligosaccharides, which accounted for 74% of the total N-linked sugar chains released by hydrazinolysis of rat small intestinal aminopeptidase N, were investigated on a structural basis. They are mainly composed of complex-type sugar chains with tri- and tetraantennary structures, and small amounts of high man...

journal_title：Biochemistry

authors： Takasaki S,Erickson RH,Kim YS,Kochibe N,Kobata A

更新日期：1991-09-17 00:00:00

abstract：:We have investigated the loss of histone H1 from chromatin [D'Anna, J. A., Gurley, L. R., & Tobey, R. A. (1982) Biochemistry 21, 3991-4001] and the structure of chromatin from Chinese hamster (line CHO) cells blocked in early S phase by sequential use of isoleucine deprivation G1 block and 1 mM hydroxyurea (HU) blocka...

journal_title：Biochemistry

authors： D'Anna JA,Prentice DA

更新日期：1983-11-22 00:00:00

abstract：:High-risk human papillomavirus E6 participates in tumorigenic progression, mainly by its ability to promote p53 degradation. HPV transcripts show a complex splicing pattern, where E6* is the most abundant transcript in high-risk HPV types, comprising the first 50 amino acids of E6. No structural or biochemical informa...

journal_title：Biochemistry

authors： Heer A,Alonso LG,de Prat-Gay G

更新日期：2011-03-01 00:00:00

abstract：:Glutaraldehyde treatment of electroplax membrane preparations of Na,K-ATPase leads to irreversible changes in the enzymic behavior of the protein, which are not due to modification of the active site. When the glutaraldehyde treatment is carried out in a medium containing K+ and without Na+, the "K+-modified enzyme" s...

journal_title：Biochemistry

authors： Chipman DM,Lev A

更新日期：1983-09-13 00:00:00

abstract：:ATP, its nonhydrolyzable analogue, AMP-PNP, and albumin were found to promote the dissociation of rhodopsin kinase from rod outer segments (ROS) containing photoactivated-rhodopsin (Rho*). These features were embodied in a protocol for the recovery of rhodopsin kinase from incubations containing ROS which had been sub...

journal_title：Biochemistry

authors： Dean KR,Akhtar M

更新日期：1996-05-14 00:00:00

abstract：:The infectious isoform of the prion protein (PrPSc) is derived from cellular PrP (PrPC) in a conversion reaction involving a dramatic reorganization of secondary and tertiary structure. While our understanding of the pathogenic role of PrPSc has grown, the normal physiologic function of PrPC still remains unclear. Usi...

journal_title：Biochemistry

authors： Stöckel J,Safar J,Wallace AC,Cohen FE,Prusiner SB

更新日期：1998-05-19 00:00:00

abstract：:We have employed high-performance liquid chromatography (HPLC) to investigate the syntheses of histones H1 and H1o as synchronized cells traverse from mitosis to S phase. Chinese hamster (line CHO) cells were synchronized by mitotic selection, and, at appropriate times, they were pulse labeled for 1 h with [3H]lysine....

journal_title：Biochemistry

authors： D'Anna JA,Thayer MM,Tobey RA,Gurley LR

更新日期：1985-04-09 00:00:00

abstract：:Tat, an essential human immunodeficiency virus type 1 protein interacts with the transactivation response element (TAR) and stimulates transcription from the viral long-terminal repeat (LTR). Blockage of Tat-TAR interaction halts viral transcription and hence replication. We have found that polyamide nucleic acid (PNA...

journal_title：Biochemistry

authors： Mayhood T,Kaushik N,Pandey PK,Kashanchi F,Deng L,Pandey VN

更新日期：2000-09-26 00:00:00

abstract：:The ATPase activity of the (Ca2+-Mg2+)-ATPase reconstituted into bilayers of phosphatidylcholines depends on the fatty acyl chain length of the phospholipids. It is shown that the fluorescence response to Ca2+ of the ATPase modified with fluorescein isothiocyanate is also dependent on phospholipid structure and is int...

journal_title：Biochemistry

authors： Froud RJ,East JM,Jones OT,Lee AG

更新日期：1986-11-18 00:00:00

abstract：:To better understand origin recognition and initiation of DNA replication, we have examined by NMR complexes formed between the origin-binding domain of SV40 T antigen (T-ag-obd), the initiator protein of the SV40 virus, and cognate and noncognate DNA oligomers. The results reveal two structural effects associated wit...

journal_title：Biochemistry

authors： Bradshaw EM,Sanford DG,Luo X,Sudmeier JL,Gurard-Levin ZA,Bullock PA,Bachovchin WW

更新日期：2004-06-08 00:00:00

abstract：:The pyridoxal 5'-phosphate dependent histidine decarboxylase from Morganella morganii shows a nitrogen isotope effect k14/k15 = 0.9770 +/- 0.0021, a carbon isotope effect k12/k13 = 1.0308 +/- 0.0006, and a carbon isotope effect for L-[alpha-2H]histidine of 1.0333 +/- 0.0001 at pH 6.3, 37 degrees C. These results indic...

journal_title：Biochemistry

authors： Abell LM,O'Leary MH

更新日期：1988-08-09 00:00:00

abstract：:Achieving a satisfactory biochemical explanation for the opportunistic underwater adhesion of marine invertebrates such as mussels and barnacles requires a detailed characterization of proteins extracted from holdfast structures produced by these organisms. Mefp-5 is an adhesive protein derived from the foot of the co...

journal_title：Biochemistry

authors： Waite JH,Qin X

更新日期：2001-03-06 00:00:00

abstract：:Histone H10 differs substantially from H1 in a 37-residue region of the primary structure that is conserved between the usual H1 histones of fish and mammals. Instead, H10 is homologous to H5 in this region. Histones H10 and H5 might be classified together in a histone category distinct from the usual H1. ...

journal_title：Biochemistry

authors： Pehrson JR,Cole RD

更新日期：1981-04-14 00:00:00

abstract：:X-ray absorption spectroscopy has been used to investigate binding of selenohomocysteine to cobalamin-independent (MetE) and cobalamin-dependent (MetH) methionine synthase enzymes of Escherichia coli. We have shown previously [Peariso et al. (1998) J. Am. Chem. Soc. 120, 8410-8416] that the Zn sites in both enzymes sh...

journal_title：Biochemistry

authors： Peariso K,Zhou ZS,Smith AE,Matthews RG,Penner-Hahn JE

更新日期：2001-01-30 00:00:00

abstract：:The solvent polarity dependence of the interaction between thiamin and tryptophan was studied by spectrophotometric methods. The ultraviolet (UV) data clearly indicate that the interaction is weakened when the complex is transferred from water to aqueous ethanol or aqueous dioxane. The interaction of thiamin and trypt...

journal_title：Biochemistry

authors： Farzami B,Mariam YH,Jordan F

更新日期：1977-03-22 00:00:00

abstract：:The formation of amyloid fibril is associated with major human diseases, including Alzheimer's disease, prion diseases, and type 2 diabetes. Methods for efficient inhibition of amyloid fibril formation are therefore highly clinically important. A principal approach for the inhibition of amyloid formation is based on t...

journal_title：Biochemistry

authors： Porat Y,Mazor Y,Efrat S,Gazit E

更新日期：2004-11-16 00:00:00

abstract：:Many investigations have revealed that ribosome numbers increase in parallel with the growth rate of cells. Here we show that the absolute level of protein synthesis may not be the only factor influencing rRNA synthesis in a nondividing eukaryotic cell. Under conditions of complete (greater than 99%) inhibition of pro...

journal_title：Biochemistry

authors： Yamamoto KK,Pellegrini M

更新日期：1990-12-18 00:00:00

abstract：:Analogues of tri- and tetrapeptide substrates of carboxypeptidase A in which the scissile peptide linkage is replaced with a phosphonate moiety (-PO2--O-) were synthesized and evaluated as inhibitors of the enzyme. The inhibitors terminated with either L-lactate or L-phenyllactate [designated (O) Ala and (O) Phe, resp...

journal_title：Biochemistry

authors： Hanson JE,Kaplan AP,Bartlett PA

更新日期：1989-07-25 00:00:00

abstract：:Minibrain kinase/dual-specificity tyrosine phosphorylation regulated kinase 1A (MNB/DYRK1A) is a proline/arginine-directed serine/threonine kinase implicated in the learning deficits of Down syndrome. Epigallocatechin-3-gallate (EGCG), the major tea polyphenolic compound, is a potent MNB/DYRK1A inhibitor. In this stud...

journal_title：Biochemistry

authors： Adayev T,Chen-Hwang MC,Murakami N,Wegiel J,Hwang YW

更新日期：2006-10-03 00:00:00

abstract：:The effect of normo (NTG)- and hypertriglyceridemic (HTG)-VLDL on cultured human umbilical vein endothelial cell (HUVEC) surface-localized fibrinolysis was examined following pre-incubation with NTG-, HTG-VLDL, LDL (1-20 micrograms/mL) or buffer (control). Ligand binding assays, using 125I-labeled tcu-PA, t-PA, or Glu...

journal_title：Biochemistry

authors： Li XN,Koons JC,Benza RL,Parks JM,Varma VK,Bradley WA,Gianturco SH,Taylor KB,Grammer JR,Tabengwa EM,Booyse FM

更新日期：1996-05-14 00:00:00

abstract：:A 150-kDa DNA-dependent ATPase composed of 83/68-kDa subunits was previously reported to cofractionate with a 21S complex of enzymes for DNA synthesis from HeLa cells (Vishwanatha, J. K., & Baril, E. F. (1990) Biochemistry 29, 8753-8759). The DNA-dependent ATPase was purified to electrophoretic homogeneity from a HeLa...

journal_title：Biochemistry

authors： Cao QP,Pitt S,Leszyk J,Baril EF

更新日期：1994-07-19 00:00:00

abstract：:The bacteriophage T7 DNA helicase/primase (gene 4 protein) is a ring-like hexamer that encircles ssDNA and requires forked DNA to catalyze DNA unwinding. We report that optimal rates of unwinding of forked DNA require ssDNA tails of 55 nucleotides on the 5'-to-3' strand and 15 nucleotides on the 3'-to-5' strand. Surpr...

journal_title：Biochemistry

authors： Hacker KJ,Johnson KA

更新日期：1997-11-18 00:00:00

abstract：:Six rhodopsin mutants containing disulfide cross-links between different cytoplasmic regions were prepared: disulfide bond 1, between Cys65 (interhelical loop I-II) and Cys316 (end of helix VII); disulfide bond 2, between Cys246 (end of helix VI) and Cys312 (end of helix VII); disulfide bond 3, between Cys139 (end of ...

journal_title：Biochemistry

authors： Cai K,Klein-Seetharaman J,Hwa J,Hubbell WL,Khorana HG

更新日期：1999-09-28 00:00:00

abstract：:Numerous studies have concluded that the interaction of the amyloid β-peptide (Aβ) and cellular membranes contributes to the toxicity and cell death observed in the progression of Alzheimer's disease. Aggregated Aβ species disrupt membranes, leading to physical instability and ion leakage. Further, the presence of Aβ ...

journal_title：Biochemistry

authors： Lemkul JA,Bevan DR

更新日期：2013-07-23 00:00:00

abstract：:The melibiose permease of Salmonella typhimurium (MelB(St)) catalyzes symport of melibiose with Na(+), Li(+), or H(+), and bioinformatics analysis indicates that a conserved Gly117 (helix IV) is part of the Na(+)-binding site. We mutated Gly117 to Ala, Pro, Trp, or Arg; the effects on melibiose transport and binding o...

journal_title：Biochemistry

authors： Guan L,Jakkula SV,Hodkoff AA,Su Y

更新日期：2012-04-03 00:00:00

abstract：:Multisite mutagenesis of single-chain and monomeric forms of human interleukin 5 (IL-5) was performed to investigate mechanistic features of receptor activation and the possibility of differentiating sites of activation from those for receptor interaction. The normally dimeric human IL-5 contains two domains, each con...

journal_title：Biochemistry

authors： Plugariu CG,Wu SJ,Zhang W,Chaiken I

更新日期：2000-12-05 00:00:00

abstract：:The laminin alpha3 chain is mainly expressed at the skin, and its C-terminal G domain has a critical role in multiple biological functions. We screened for biologically active sites on the mouse laminin alpha3 chain G domain using 107 synthetic peptides on coated plates and conjugated to Sepharose beads with HT1080 hu...

journal_title：Biochemistry

authors： Urushibata S,Katagiri F,Takaki S,Yamada Y,Fujimori C,Hozumi K,Kikkawa Y,Kadoya Y,Nomizu M

更新日期：2009-11-10 00:00:00