Biologically active sequences in the mouse laminin alpha3 chain G domain.

abstract：:Plasminogen is known to undergo an extremely large conformational change when it binds ligands; the two well-established conformations are either closed (absence of external ligand) or open (presence of external ligand). We show here that plasminogen is more complicated than can be accommodated by a two-state, closed/...

journal_title：Biochemistry

authors： Kornblatt JA,Schuck P

更新日期：2005-10-04 00:00:00

abstract：:Factor XIIIa (a2') is a homodimeric transglutaminase that is formed via limited alpha-thrombin-catalyzed proteolysis of the platelet (a2) or plasma (a2b2) factor XIII zymogen in a reaction that results in proteolytic removal of a 37-aminoacyl residue peptide from the N-terminus of the a chains and exposure of the acti...

journal_title：Biochemistry

authors： Hornyak TJ,Shafer JA

更新日期：1992-01-21 00:00:00

abstract：:Murine class alpha glutathione S-transferase subunit types A2 (mGSTA2-2) and A1 (mGSTA1-1) have high catalytic efficiency for glutathione (GSH) conjugation of the ultimate carcinogenic metabolite of benzo[a]pyrene, (+)-anti-7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene, [(+)-anti-BPDE]. Only 10 residues dif...

journal_title：Biochemistry

authors： Gu Y,Xiao B,Wargo HL,Bucher MH,Singh SV,Ji X

更新日期：2003-02-04 00:00:00

abstract：:Previous investigations have shown that bovine growth hormone (bGH, somatotropin) unfolds through a reversible multistate process with at least one stable equilibrium intermediate. In extending our knowledge of the folding process for bGH, we demonstrate that a self-associated form of partially denatured bGH is formed...

journal_title：Biochemistry

authors： Havel HA,Kauffman EW,Plaisted SM,Brems DN

更新日期：1986-10-21 00:00:00

abstract：:Beta-catenin phosphorylation at serine 45 (Ser45), threonine 41 (Thr41), Ser37, and Ser33 is critical for beta-catenin degradation, and regulation of beta-catenin phosphorylation is a central part of the canonical Wnt signaling pathway. Beta-catenin mutations at Ser45, Thr41, Ser37, and Ser33 perturb beta-catenin degr...

journal_title：Biochemistry

authors： Wu G,He X

更新日期：2006-04-25 00:00:00

abstract：:Oligothymidylates covalently linked to iron-porphyrins were synthesized to target the nuclease activity of Fe-porphyrin to complementary polynucleotides. In the presence of oxygen and a reducing agent, oligo(dT)7 bearing the reactive group attached to the 3'-phosphate was shown to be active in the cleavage of poly(dA)...

journal_title：Biochemistry

authors： Le Doan T,Perrouault L,Helene C,Chassignol M,Thuong NT

更新日期：1986-11-04 00:00:00

abstract：:Myeloperoxidase plays a fundamental role in oxidant production by neutrophils. The enzyme uses hydrogen peroxide to oxidize chloride (Cl-), bromide (Br-), iodide (I-), and the pseudohalide thiocyanate (SCN-) to their respective hypohalous acids. This study for the first time presents transient kinetic measurements of ...

journal_title：Biochemistry

authors： Furtmüller PG,Burner U,Obinger C

更新日期：1998-12-22 00:00:00

abstract：:The fungal neurotoxin alpha-cyclopiazonic acid (CPA), a nanomolar inhibitor of Ca2+-ATPase, has a pentacyclic indole tetramic acid scaffold that arises from one molecule of tryptophan, acetyl-CoA, malonyl-CoA, and dimethylallyl pyrophosphate by consecutive action of three enzymes, CpaS, CpaD, and CpaO. CpaS is a hybri...

journal_title：Biochemistry

authors： Liu X,Walsh CT

更新日期：2009-09-15 00:00:00

abstract：:RS1, also known as retinoschisin, is an extracellular discoidin domain-containing protein that has been implicated in maintaining the cellular organization and synaptic structure of the vertebrate retina. Mutations in the gene encoding RS1 are responsible for X-linked retinoschisis, a retinal degenerative disease char...

journal_title：Biochemistry

authors： Dyka FM,Wu WW,Pfeifer TA,Molday LL,Grigliatti TA,Molday RS

更新日期：2008-09-02 00:00:00

abstract：:CD6 is a member of the scavenger receptor cysteine rich protein superfamily (SRCRSF). This family includes many cell surface proteins whose three-dimensional structures and functions are presently not well understood. The extracellular region of CD6 includes 3 SRCR domains. The membrane proximal SRCR domain specifical...

journal_title：Biochemistry

authors： Bodian DL,Skonier JE,Bowen MA,Neubauer M,Siadak AW,Aruffo A,Bajorath J

更新日期：1997-03-04 00:00:00

abstract：:The structures of two RNA duplexes, whose sequences correspond to portions of the ColE1 plasmid copy control RNA I and RNA II, have been determined. Crystals containing the 18mers 5'-CA CCGUUGGUAGCGGUGC-3' and 5'-CACCGCUACCAACGGUGC-3' diffract to 1.20 A resolution while those containing the 19mers 5'-GCACCGUUGGUAGCGGU...

journal_title：Biochemistry

authors： Klosterman PS,Shah SA,Steitz TA

更新日期：1999-11-09 00:00:00

abstract：:Mutation of polycystin-1 (PC1) is the major cause of autosomal dominant polycystic kidney disease. PC1 has a predicted molecular mass of ~460 kDa comprising a long multidomain extracellular N-terminal region, 11 transmembrane regions, and a short C-terminal region. Because of its size, PC1 has proven difficult to hand...

journal_title：Biochemistry

authors： Oatley P,Stewart AP,Sandford R,Edwardson JM

更新日期：2012-04-03 00:00:00

abstract：:The folding of the two-domain bacterial chaperone PapD has been studied to develop an understanding of the relationship between individual domain folding and the formation of domain-domain interactions. PapD contains six phenylalanine residues, four in the N-terminal domain and two in the C-terminal domain. To examine...

journal_title：Biochemistry

authors： Bann JG,Frieden C

更新日期：2004-11-02 00:00:00

abstract：:We studied the interactions between the SH2 domain of growth factor receptor binding protein 2 (Grb2) and ErbB receptor-derived phosphotyrosyl peptides using molecular dynamics, free energy calculations, and surface plasmon resonance (SPR) analysis. Binding free energies for nine phosphotyrosyl peptides were calculate...

journal_title：Biochemistry

authors： Suenaga A,Hatakeyama M,Ichikawa M,Yu X,Futatsugi N,Narumi T,Fukui K,Terada T,Taiji M,Shirouzu M,Yokoyama S,Konagaya A

更新日期：2003-05-13 00:00:00

abstract：:Classical molecular dynamics simulations were utilized to investigate the structural and dynamical properties of water in the active site of ketosteroid isomerase (KSI) to provide insight into the role of these water molecules in the enzyme-catalyzed reaction. This reaction is thought to proceed via a dienolate interm...

journal_title：Biochemistry

authors： Hanoian P,Hammes-Schiffer S

更新日期：2011-08-09 00:00:00

abstract：:Lipid-protein interactions in bovine rod outer segment disk membranes have been studied by using a series of eight stearic acid spin-label probes which were labeled at different carbon atom positions in the chain. In randomly oriented membrane dispersions, the electron spin resonance (ESR) spectra of the C-8, C-9, C-1...

journal_title：Biochemistry

authors： Pates RD,Marsh D

更新日期：1987-01-13 00:00:00

abstract：:Ribonucleotide reductase (RDPR) from Escherichia coli is composed of two subunits, R1 and R2, both of which are required to catalyze the conversion of nucleotides to deoxynucleotides. This reduction process is accompanied by oxidation of two cysteines within the active site to a disulfide. One of these putative active...

journal_title：Biochemistry

authors： Mao SS,Holler TP,Bollinger JM Jr,Yu GX,Johnston MI,Stubbe J

更新日期：1992-10-13 00:00:00

abstract：:We detect internal water molecules in a membrane-embedded receptor-transducer complex and demonstrate water structure changes during formation of the signaling state. Time-resolved FTIR spectroscopy reveals stimulus-induced repositioning of one or more structurally active water molecules to a significantly more hydrop...

journal_title：Biochemistry

authors： Bergo VB,Spudich EN,Spudich JL,Rothschild KJ

更新日期：2009-02-10 00:00:00

abstract：:Heme uptake and utilization by pathogenic bacteria are critical for virulence and disease, since heme and heme proteins are a major source of iron within the host. Although the role of outer membrane heme receptors in this process has been extensively characterized at the genetic and biochemical level, the role of the...

journal_title：Biochemistry

authors： Kaur AP,Wilks A

更新日期：2007-03-20 00:00:00

abstract：:Hydrogen bonds which form between a hydrogen bond donor and an aromatic ring as acceptor are thought to contribute to the stability and function of proteins. We have tested the function of such an interaction in a highly homologous pair of proteins, cellular retinol-binding protein (CRBP) and cellular retinol-binding ...

journal_title：Biochemistry

authors： Jamison RS,Kakkad B,Ebert DH,Newcomer ME,Ong DE

更新日期：1995-09-05 00:00:00

abstract：:Bacterial bioluminescence is a phenomenon resulting from the reaction of a two-component FMN-dependent aldehyde monooxygenase system, which comprises a bacterial luciferase and a flavin reductase. Bacterial luciferase (LuxAB) is one of the most extensively investigated two-component monooxygenases, while its reductase...

journal_title：Biochemistry

authors： Nijvipakul S,Ballou DP,Chaiyen P

更新日期：2010-11-02 00:00:00

abstract：:Ultraviolet resonance Raman (UV RR) spectra are reported for ferricytochrome c from tuna and horse heart at pH 1.6, 7, 10, and 13, representing distinct conformational states of the protein (states II, III, IV, and V, respectively). The spectra were obtained with pulsed laser excitation at 200 and 218 nm, via H2 Raman...

journal_title：Biochemistry

authors： Copeland RA,Spiro TG

更新日期：1985-08-27 00:00:00

abstract：:The reaction catalyzed by orotidine 5'-monophosphate decarboxylase (OMPDC) is accompanied by exceptional values for rate enhancement (k(cat)/k(non) = 7.1 × 10(16)) and catalytic proficiency [(k(cat)/K(M))/k(non) = 4.8 × 10(22) M(-1)]. Although a stabilized vinyl carbanion/carbene intermediate is located on the reactio...

journal_title：Biochemistry

authors： Iiams V,Desai BJ,Fedorov AA,Fedorov EV,Almo SC,Gerlt JA

更新日期：2011-10-04 00:00:00

abstract：:The mechanisms of MRP1-drug binding and transport are not clear. In this study, we have characterized the interaction between MRP1 and rhodamine 123 (Rh123) using the photoreactive-iodinated analogue, [(125)I]iodoaryl azido-rhodamine 123 (or IAARh123). Photoaffinity labeling of plasma membranes from HeLa cells transfe...

journal_title：Biochemistry

authors： Daoud R,Kast C,Gros P,Georges E

更新日期：2000-12-19 00:00:00

abstract：:Calpains are intracellular calcium-activated cysteine proteases whose unregulated proteolysis following the loss of calcium homeostasis can lead to acute degeneration during ischemic episodes and trauma, as well as Alzheimer's disease and cataract formation. The determination of the crystal structure of the proteolyti...

journal_title：Biochemistry

authors： Cuerrier D,Moldoveanu T,Inoue J,Davies PL,Campbell RL

更新日期：2006-06-20 00:00:00

abstract：:The enzyme form of the calcium adenosinetriphosphatase of sarcoplasmic reticulum (CaATPase) that is stable in the presence of calcium, cE.Ca2, has a binding site for the catalytic Mg2+ ion with a dissociation constant of 0.94 +/- 0.15 mM at 25 degrees C, pH 7.0, and 100 mM KCl. This is approximately 10 times smaller t...

journal_title：Biochemistry

authors： Reinstein J,Jencks WP

更新日期：1993-07-06 00:00:00

abstract：:The photoreceptor protein rhodopsin has been reconstituted with a single phospholipid species, dimyristoylphosphatidylcholine, at a range of different lipid/protein ratios, and the exchange rate at the lipid-protein interface has been determined from the electron spin resonance spectra of spin-labeled phosphatidylchol...

journal_title：Biochemistry

authors： Ryba NJ,Horváth LI,Watts A,Marsh D

更新日期：1987-06-02 00:00:00

abstract：:The acetylenic analogue of adenosine 9-(5',6'-dideoxy-beta-D-ribo-hex-5'-ynofuranosyl)adenine has been synthesized, and its behavior as an inhibitor of bovine S-adenosylhomocysteine hydrolase has been examined. Incubation of the enzyme with excess inhibitor caused a time-dependent, irreversible inactivation of the enz...

journal_title：Biochemistry

authors： Parry RJ,Muscate A,Askonas LJ

更新日期：1991-10-15 00:00:00

abstract：:The microtubule-associated protein tau, in a hyperphosphorylated form, aggregates into insoluble paired-helical filaments (PHFs) in Alzheimer's disease (AD) and other tauopathies. In AD, there is approximately 8 mol of phosphate per mole of tau distributed among approximately 30 PHF phosphorylation sites as compared t...

journal_title：Biochemistry

authors： Sun Q,Gamblin TC

更新日期：2009-06-30 00:00:00

abstract：:N-Methylpurine-DNA glycosylase (MPG), a ubiquitous DNA repair enzyme, is responsible for the removal of a wide variety of alkylated base lesions in DNA, e.g., N-alkylpurines and cyclic ethenoadducts of adenine, guanine, and cytosine. These lesions, some of which are mutagenic and toxic, are generated endogenously or b...

journal_title：Biochemistry

authors： Roy R,Biswas T,Hazra TK,Roy G,Grabowski DT,Izumi T,Srinivasan G,Mitra S

更新日期：1998-01-13 00:00:00