Abstract：
:Oligothymidylates covalently linked to iron-porphyrins were synthesized to target the nuclease activity of Fe-porphyrin to complementary polynucleotides. In the presence of oxygen and a reducing agent, oligo(dT)7 bearing the reactive group attached to the 3'-phosphate was shown to be active in the cleavage of poly(dA) and poly(rA) but not poly(dT). When poly(dA) was used as a matrix, the reaction yield was higher at low temperature where the complexes are stable; upon increasing temperature, the reaction yield decreased in agreement with the dissociation of the oligonucleotide-polynucleotide complex as measured by absorption spectroscopy. Thus, oligonucleotides covalently linked to iron-porphyrin derivatives can be used to cleave selectively the target sequence of the oligonucleotide.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Le Doan T,Perrouault L,Helene C,Chassignol M,Thuong NTdoi
10.1021/bi00370a002subject
Has Abstractpub_date
1986-11-04 00:00:00pages
6736-9issue
22eissn
0006-2960issn
1520-4995journal_volume
25pub_type
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