Mapping the bimolecular interface of the parathyroid hormone (PTH)-PTH1 receptor complex: spatial proximity between Lys(27) (of the hormone principal binding domain) and leu(261) (of the first extracellular loop) of the human PTH1 receptor.

abstract：:Previous studies have shown that the short-motif electroplax Na channel is phosphorylated in vitro by cyclic AMP-dependent protein kinase (PKA) at serines 6 or 7 and 1776 and threonine 17 (Emerick & Agnew, 1989). We here show that phosphatase treatment of solubilized, purified Na channels enhanced subsequent PKA label...

journal_title：Biochemistry

authors： Emerick MC,Shenkel S,Agnew WS

更新日期：1993-09-14 00:00:00

abstract：:13C NMR has been used to demonstrate the metabolism of dilute solutions of labeled formaldehyde by Escherichia coli to methanol, formate, carbon dioxide, and several other unidentified metabolites which contain labeled CH2 groups. Aeration of bacterial suspensions within the spectrometer dramatically increased the rat...

journal_title：Biochemistry

authors： Hunter BK,Nicholls KM,Sanders JK

更新日期：1984-01-31 00:00:00

abstract：:The nonbulky 5',8-cyclopurine DNA lesions (cP) and the bulky, benzo[ a]pyrene diol epoxide-derived stereoisomeric cis- and trans- N2-guanine adducts (BPDE-dG) are good substrates of the human nucleotide excision repair (NER) mechanism. These DNA lesions were embedded at the In or Out rotational settings near the dyad ...

journal_title：Biochemistry

authors： Shafirovich V,Kolbanovskiy M,Kropachev K,Liu Z,Cai Y,Terzidis MA,Masi A,Chatgilialoglu C,Amin S,Dadali A,Broyde S,Geacintov NE

更新日期：2019-02-12 00:00:00

abstract：:Sulfhydryl-reactive derivatives of glucosamine were synthesized as potentially transportable affinity labels of the human erythrocyte hexose carrier. N-Maleoylglycyl derivatives of either 6- or 2-amino-2-deoxy-D-glucopyranose were the most potent inhibitors of 3-O-methylglucose uptake, with concentrations of half-maxi...

journal_title：Biochemistry

authors： May JM

更新日期：1989-02-21 00:00:00

abstract：:Hepatic mixed-function oxidase metabolism of the ubiquitous pollutant polychlorinated biphenyls (PCBs) is implicated in their toxification and detoxification. We used dichlorobiphenyls (DCBs) as models to investigate the effect of the chloro substituent sites on this metabolism experimentally and by molecular orbital ...

journal_title：Biochemistry

authors： Kaminsky LS,Kennedy MW,Adams SM,Guengerich FP

更新日期：1981-12-22 00:00:00

abstract：:Gelatinase B is a matrix metalloproteinase (MMP-9) involved in tissue remodeling, development, cancer, and inflammation. Neutrophils produce three major forms of (pro)gelatinase B: 92 kDa monomers, homodimers, and complexes of gelatinase B covalently bound to neutrophil gelatinase B-associated lipocalin (NGAL). In con...

journal_title：Biochemistry

authors： Rudd PM,Mattu TS,Masure S,Bratt T,Van den Steen PE,Wormald MR,Küster B,Harvey DJ,Borregaard N,Van Damme J,Dwek RA,Opdenakker G

更新日期：1999-10-19 00:00:00

abstract：:Furanacryloyl-Phe-Gly-Gly has been shown to be a convenient substrate for angiotensin converting enzyme (dipeptidyl carboxypeptidase, EC 3.4.15.1). A detailed kinetic analysis of the hydrolysis of this substrate indicates normal Michaelis-Menten behavior with kcat = 19000 min-1 and KM = 3.0 x 10(-4) M determined at pH...

journal_title：Biochemistry

authors： Bünning P,Holmquist B,Riordan JF

更新日期：1983-01-04 00:00:00

abstract：:Heme uptake and utilization by pathogenic bacteria are critical for virulence and disease, since heme and heme proteins are a major source of iron within the host. Although the role of outer membrane heme receptors in this process has been extensively characterized at the genetic and biochemical level, the role of the...

journal_title：Biochemistry

authors： Kaur AP,Wilks A

更新日期：2007-03-20 00:00:00

abstract：:The structural determinants of substrate specificity of human liver cytochrome P450 2C8 (CYP2C8) were investigated using site-directed mutants chosen on the basis of a preliminary substrate pharmacophore and a three-dimensional (3D) model. Analysis of the structural features common to CYP2C8 substrates exhibiting a mi...

journal_title：Biochemistry

authors： Melet A,Marques-Soares C,Schoch GA,Macherey AC,Jaouen M,Dansette PM,Sari MA,Johnson EF,Mansuy D

更新日期：2004-12-14 00:00:00

abstract：:The ErbB family of tyrosine kinase receptors is a key element in preserving cell growth homeostasis. This family is comprised of four single-transmembrane domain proteins designated ErbB-1-4. Ligand binding initiates dimerization followed by tyrosine phosphorylation and signaling, which when uncontrolled lead to cance...

journal_title：Biochemistry

authors： Bublil EM,Cohen T,Arnusch CJ,Peleg A,Pines G,Lavi S,Yarden Y,Shai Y

更新日期：2016-10-04 00:00:00

abstract：:Heme-regulated eIF2alpha kinase [heme-regulated inhibitor (HRI)] plays a critical role in the regulation of protein synthesis by heme iron. The kinase active site is located in the C-terminal domain, whereas the N-terminal domain is suggested to regulate catalysis in response to heme binding. Here, we found that the r...

journal_title：Biochemistry

authors： Miksanova M,Igarashi J,Minami M,Sagami I,Yamauchi S,Kurokawa H,Shimizu T

更新日期：2006-08-15 00:00:00

abstract：:Recombinant human 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthetase, isoform 1 (brain), was purified to near-homogeneity from an Escherichia coli expression system and kinetically characterized. The native enzyme, a dimer with each 71 kDa subunit containing an adenosine triphosphate (ATP) sulfurylase and an aden...

journal_title：Biochemistry

authors： Lansdon EB,Fisher AJ,Segel IH

更新日期：2004-04-13 00:00:00

abstract：:The kinetics of human polymerase beta (pol beta) binding to the single-stranded DNA, in the (pol beta)(16) and (pol beta)(5) binding modes, that differ in the number of occluded nucleotide residues in the protein-DNA complexes, have been examined, using the fluorescence stopped-flow technique. This is the first determ...

journal_title：Biochemistry

authors： Rajendran S,Jezewska MJ,Bujalowski W

更新日期：2001-10-02 00:00:00

abstract：:The modulatory action of Ca2+-calmodulin on multiple targets is inhibited by trifluoperazine, which competes with target proteins for calmodulin binding. The structure of calmodulin crystallized with two trifluoperazine molecules is determined by X-ray crystallography at 2.74 A resolution. The X-ray data together with...

journal_title：Biochemistry

authors： Vertessy BG,Harmat V,Böcskei Z,Náray-Szabó G,Orosz F,Ovádi J

更新日期：1998-11-03 00:00:00

abstract：:Using the thrombin-cut [68-30 kilodalton (kDa)] myosin subfragment 1 (S-1) whose heavy chain has been selectively split within the central 50-kDa region, at Lys-560, with concomitant specific alterations of the ATPase and actin binding properties [Chaussepied, P., Mornet, D., Audemard, E., Derancourt, J., & Kassab, R....

journal_title：Biochemistry

authors： Chaussepied P,Mornet D,Kassab R

更新日期：1986-10-21 00:00:00

abstract：:The lipid fatty acid composition of the cytoplasmic membranes of Escherichia coli can be varied by growing an unsaturated fatty acid auxotroph in the presence of different fatty acid supplements. Electron spin resonance (ESR) studies of spin-label partitioning into the cytoplasmic membranes of different lipid fatty ac...

journal_title：Biochemistry

authors： Linden CD,Blasie JK,Fox CF

更新日期：1977-04-19 00:00:00

abstract：:Amyloid diseases make up a set of fatal disorders in which proteins aggregate to form fibrils that deposit in tissues throughout the body. Amyloid-associated diseases are challenging to study because amyloid formation occurs on time scales that span several orders of magnitude and involve heterogeneous, interconvertin...

journal_title：Biochemistry

authors： Childers MC,Daggett V

更新日期：2019-11-05 00:00:00

abstract：:It was previously observed that two steps of the reaction of eukaryotic DNA topoisomerase I (topoisomerization and cleavage) depend upon the conformation of the DNA substrate: in both instances the supercoiled form is a more efficient substrate than the relaxed one. This paper reports the analysis of two other steps o...

journal_title：Biochemistry

authors： Caserta M,Amadei A,Camilloni G,Di Mauro E

更新日期：1990-09-04 00:00:00

abstract：:Many biological processes are now known to be regulated by Ca2+ via calmodulin (CM). Although a general mechanistic model by which Ca2+ and calmodulin modulate many of these activities has been proposed, an accurate quantitative model is not available. A detailed analysis of skeletal muscle myosin light chain kinase a...

journal_title：Biochemistry

authors： Blumenthal DK,Stull JT

更新日期：1980-11-25 00:00:00

abstract：:Human S-adenosylmethionine decarboxylase (AdoMetDC) was expressed in high yield in Escherichia coli using the pIN-III(lppP-5) expression vector and purified to apparent homogeneity using affinity chromatography on methylglyoxal bis(guanylhydrazone)-Sepharose. The inactivation of the purified enzyme by 5'-deoxy-5'-[(3-...

journal_title：Biochemistry

authors： Shantz LM,Stanley BA,Secrist JA 3rd,Pegg AE

更新日期：1992-07-28 00:00:00

abstract：:Following a 1-h [3H]uridine pulse of cells of a human colon carcinoma line, 15% of the radioactivity in heterogeneous nuclear RNA associated with both chromatin and nuclear ribonucleoprotein was not digested to acid soluble fragments during a 2-h incubation with staphylococcal nuclease (EC 3.1.4.7). These [3H]uridine-...

journal_title：Biochemistry

authors： Augenlicht LH,McCormick M,Lipkin M

更新日期：1976-08-24 00:00:00

abstract：:Firefly luciferase is 80-90% inactivated within 3 h upon incubation with the adenine nucleotide analogue p-fluorosulfonylbenzoyl-5'-adenosine (FSBA). Although 4 mol of 14C-FSBA/mol of enzyme is irreversibly bound during inactivation, only 1 mol of 14C-FSBA appears to be specifically directed to an adenine nucleotide b...

journal_title：Biochemistry

authors： Lee Y,Esch FS,DeLuca MA

更新日期：1981-03-03 00:00:00

abstract：:A computational method has been developed to predict inhibitor binding energy for untested inhibitor molecules. A neural network is trained from the electrostatic potential surfaces of known inhibitors and their binding energies. The algorithm is then able to predict, with high accuracy, the binding energy of unknown ...

journal_title：Biochemistry

authors： Braunheim BB,Miles RW,Schramm VL,Schwartz SD

更新日期：1999-12-07 00:00:00

abstract：:High-resolution nitrite soaked oxidized and reduced crystal structures of two active site mutants, D98N and H255N, of nitrite reductase (NIR) from Alcaligenes faecalis S-6 were determined to better than 2.0 A resolution. In the oxidized D98N nitrite-soaked structures, nitrite is coordinated to the type II copper via i...

journal_title：Biochemistry

authors： Boulanger MJ,Murphy ME

更新日期：2001-08-07 00:00:00

abstract：:Methods were developed for isolation of bovine cardiac titin in the apparently full-sized T1 form. The pure protein could be obtained either in the denatured state after chromatography of myofibril extracts on Sephacryl S-1000 or in the native state using hydroxylapatite chromatography. Titin-specific chicken polyclon...

journal_title：Biochemistry

authors： Pan KM,Damodaran S,Greaser ML

更新日期：1994-07-12 00:00:00

abstract：:Our previous studies revealed that interaction of fibrin with the very low density lipoprotein (VLDL) receptor plays a prominent role in transendothelial migration of leukocytes and thereby inflammation. The major goal of our subsequent studies is to establish the structural basis for this interaction. As the first st...

journal_title：Biochemistry

authors： Banerjee K,Yakovlev S,Gruschus JM,Medved L,Tjandra N

更新日期：2018-07-31 00:00:00

abstract：:Onconase (ONC) from Rana pipiens is the smallest member of the ribonuclease A (RNase A) superfamily. Despite a tertiary structure similar to RNase A, ONC is distinguished by an extremely high thermodynamic stability. In the present paper we have probed the significance of three structural regions, which exhibit struct...

journal_title：Biochemistry

authors： Arnold U,Schulenburg C,Schmidt D,Ulbrich-Hofmann R

更新日期：2006-03-21 00:00:00

abstract：:We investigated the dependence of certain properties of bile salt/lecithin mixed micelles on the molar ratio of the two components. UV absorption suggested that the micellar system is fundamentally altered on passing through a bile salt:lecithin molar ratio of 1.8:1. Differential scanning calorimetry confirmed this tr...

journal_title：Biochemistry

authors： Claffey WJ,Holzbach RT

更新日期：1981-01-20 00:00:00

abstract：:The plasma membrane serotonin transporter (SERT) has an important role in terminating serotonergic neurotransmission by re-uptake of 5-HT from the synaptic cleft. The expression of SERT on the cell surface is therefore a critical factor. In this study, we examined the role of the carboxyl terminus of SERT in trafficki...

journal_title：Biochemistry

authors： Larsen MB,Fjorback AW,Wiborg O

更新日期：2006-01-31 00:00:00

abstract：:Colchicine induces a weak assembly-independent GTPase activity in calf brain tubulin [David-Pfeuty, T., Erickson, H. P., & Pantaloni, D. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 5372-5376; Andreu, J. M., & Timasheff, S. N. (1981) Arch. Biochem. Biophys. 211, 151-157]. Kinetic analysis shows a turnover number of 2 x 10...

journal_title：Biochemistry

authors： Perez-Ramirez B,Shearwin KE,Timasheff SN

更新日期：1994-05-24 00:00:00