Abstract：
:The lipid fatty acid composition of the cytoplasmic membranes of Escherichia coli can be varied by growing an unsaturated fatty acid auxotroph in the presence of different fatty acid supplements. Electron spin resonance (ESR) studies of spin-label partitioning into the cytoplasmic membranes of different lipid fatty acid compositions as a function of temperature have been interpreted as indicating a broad order-to-disorder transition in the membrane lipids, the end points of the transition depending upon the fatty acid composition. We have utilized x-ray diffraction to confirm the ESR studies for three different fatty acid supplements (oleic, elaidic, and bromostearic). We found that the characteristic end-point temperatures detected by ESR were indeed the end-point temperatures of a broad order-to-disorder transition of the cytoplasmic membrane lipids. In addition, Patterson functions calculated from lamellar x-ray diffraction from partially oriented cytoplasmic membranes indicate a decrease in average membrane thickness upon fatty acid chain melting.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Linden CD,Blasie JK,Fox CFdoi
10.1021/bi00627a015subject
Has Abstractpub_date
1977-04-19 00:00:00pages
1621-5issue
8eissn
0006-2960issn
1520-4995journal_volume
16pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:We have modified the cytochrome b subunit of the cytochrome bc1 complex from the purple bacterium Rhodobacter sphaeroides to introduce two distinctive features of cytochrome b6 f complexes. In the first one, we have split cyt b into two polypeptides thus mimicking the organization of cyt b6 and subunit IV in the b6 f ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9813476
更新日期：1998-11-17 00:00:00
abstract：:The effects of nonylphenol and 3,5-dibutyl-4-hydroxytoluene (BHT) on the activity of the (Ca2(+)-Mg2+)-ATPase of skeletal muscle sarcoplasmic reticulum have been studied. At high concentrations, both inhibit the ATPase activity of the ATPase either in native lipid or in bilayers of dioleoylphosphatidylcholine but, at ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00464a028
更新日期：1990-03-27 00:00:00
abstract：:The E-domain of staphylococcal protein A is one of five homologous IgG-binding domains designated E, D, A, B, and C that comprise the extracellular portion of protein A. The E-domain binds tightly to Fc fragments of IgG and binds certain Fv fragments with micromolar affinity. To explore further the structural features...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961409x
更新日期：1996-12-03 00:00:00
abstract：:Reactivation of organophosphate (OP)-inhibited acetylcholinesterase (AChE) is a key objective in the treatment of OP poisoning. This study with native, wild-type, and mutant recombinant DNA-expressed AChEs, each inhibited by representative OP compounds, establishes a relationship between edrophonium acceleration of ox...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9905720
更新日期：1999-08-03 00:00:00
abstract：:The gene 5 protein (g5p) encoded by filamentous Ff phages is an ssDNA-binding protein, which binds to and sequesters the nascent ssDNA phage genome in the process of phage morphogenesis. The g5p also binds with high affinity to DNA and RNA sequences that form G-quadruplex structures. However, sequences that would form...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi030177g
更新日期：2004-03-09 00:00:00
abstract：:The apolipoproteins of insect lipophorin were dissociated in guanidinium chloride and isolated by gel permeation chromatography. Over 98% of the total lipid in lipophorin was associated with apolipophorin I (apoLp-I), thus suggesting this apolipoprotein to be the lipid binding component of the particle. ApoLp-I was de...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00442a019
更新日期：1989-08-08 00:00:00
abstract：:Calpains are intracellular calcium-activated cysteine proteases whose unregulated proteolysis following the loss of calcium homeostasis can lead to acute degeneration during ischemic episodes and trauma, as well as Alzheimer's disease and cataract formation. The determination of the crystal structure of the proteolyti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060425j
更新日期：2006-06-20 00:00:00
abstract：:Site-directed spin labeling (SDSL), the site-specific incorporation of nitroxide spin-labels into a protein, has allowed us to investigate ligand-induced conformational changes in the ligand-binding domain of human estrogen receptor alpha (hERalpha-LBD). EPR (electron paramagnetic resonance) spectroscopy of the nitrox...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035566p
更新日期：2004-02-24 00:00:00
abstract：:15N-labeled hen lysozyme has been studied by 2D and 3D NMR in order to characterize its dynamic behavior. The resonances of all main-chain amide nitrogen atoms were assigned, as were resonances of nitrogen atoms in 28 side chains. Relaxation measurements for the main-chain and arginine and tryptophan side-chain 15N nu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00012a023
更新日期：1995-03-28 00:00:00
abstract：:cis-3-Chloroacrylic acid dehalogenase (cis-CaaD) from Pseudomonas pavonaceae 170 and a homologue from Corynebacterium glutamicum designated Cg10062 are 34% identical in sequence (54% similar). The former catalyzes a key step in a bacterial catabolic pathway for the nematocide 1,3-dichloropropene, whereas the latter ha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi4004414
更新日期：2013-06-18 00:00:00
abstract：:Eosinophil cationic protein (ECP) is a component of the eosinophil granule matrix. It shows marked toxicity against helminth parasites, bacteria single-stranded RNA viruses, and host epithelial cells. Secretion of human ECP is related to eosinophil-associated allergic, asthmatic, and inflammatory diseases. ECP belongs...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0264521
更新日期：2002-10-08 00:00:00
abstract：:Four mechanisms for the allosteric regulation of the calcium and magnesium ion activated adenosinetriphosphatase (Ca,Mg-ATPase) of sarcoplasmic reticulum were examined. Negative cooperativity in substrate binding was not supported by 3H-labeled 5'-adenylyl methylenediphosphate (AMPPCP) binding, which was best fit by a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00341a049
更新日期：1985-09-24 00:00:00
abstract：:Oxygen binding curves of sol-gel-encapsulated deoxy human adult hemoglobin (HbA) have previously revealed two distinct noncooperative populations with oxygen binding affinities approximately 1000 and 100 times lower than that of the high-affinity R state. The two populations which have been termed the low-affinity (LA...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048531d
更新日期：2004-11-02 00:00:00
abstract：:Carbamoylation and reductive methylation of tubulin have been shown previously to inhibit microtubule assembly, probably by attack on essential internal lysine residues [Mellado, W., Slebe, J., & Maccioni, R.B. (1982) Biochem. J. 203, 675-681; Szasz, J., Burns, R., & Sternlicht, H. (1982) J. Biol. Chem. 257, 3697-3704...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00403a027
更新日期：1988-02-09 00:00:00
abstract：:Conformational distributions of a four-way DNA junction have been examined by time-resolved fluorescence resonance energy transfer (FRET). A series of dye-labeled junctions were synthesized with donor (fluorescein) and acceptor (tetramethylrhodamine) dyes conjugated to the 5' termini of the duplex arms in all six pair...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00213a014
更新日期：1993-12-21 00:00:00
abstract：:The aminoglycoside 6'-N-acetyltransferase AAC(6')-Ii from Enterococcus faecium is an important microbial resistance determinant and a member of the GCN5-related N-acetyltransferase (GNAT) superfamily. We report here the further characterization of this enzyme in terms of the kinetic mechanism of acetyl transfer and id...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034148h
更新日期：2003-06-03 00:00:00
abstract：:The biosynthesis of osteocalcin (OC), a bone-specific, noncollagenous protein, is stringently regulated during differentiation of the osteoblast phenotype. Glucocorticoids, and also 1,25(OH)2D3, mediate the developmental regulation of OC gene transcription. In this study, we established that the -1097 to +23 promoter ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00093a022
更新日期：1993-10-26 00:00:00
abstract：:Despite recognition that the plasma membrane (PM) is comprised of lipid raft domains that are key organizing sites of multiple signaling pathways and other cell functions, limited information is available regarding the structure and function in sterol dynamics of these microdomains. To begin to resolve these issues, M...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0602720
更新日期：2006-10-03 00:00:00
abstract：:The insulin hexamer is an allosteric protein which displays positive and negative cooperativity and half-site reactivity that is modulated by strong homotropic and heterotropic ligand binding interactions at two different loci. These loci consist of phenolic pockets situated on the dimer-dimer interfaces of T-R and R-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9701639
更新日期：1997-08-12 00:00:00
abstract：:Chicken histone H4, labeled separately at Met-84 with N-[[(iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonic acid and 5-(iodoacetamido)fluorescein, was reassociated with unlabeled histones H2A, H2B, and H3 and 146 base pairs of DNA to produce fluorescently labeled nucleosomes having physical characteristics virtually...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00366a010
更新日期：1986-09-09 00:00:00
abstract：:Electronic absorption and resonance Raman spectra, together with rates of arsenolysis, are used to show that the acylated subunits of the rabbit furylacryloylglyceraldehyde-3-phosphate dehydrogenase (FA-rabbit enzyme), but not the FA-sturgeon enzyme, exist as a mixed population of at least two forms. One form of the F...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00515a023
更新日期：1981-06-09 00:00:00
abstract：:Binding of Ca(2+)-activated calmodulin (Ca(2+)-CaM) to neuronal nitric-oxide synthase (nNOS) increases the rate of 2,6-dichloroindolphenol (DCIP) reduction 2-3-fold and that of cytochrome c(3+) 10-20-fold. Parallel initial velocity patterns indicated that both substrates were reduced via two-half reactions in a ping-p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0023495
更新日期：2001-04-17 00:00:00
abstract：:In the present report we studied the interaction between the skeletal muscle ryanodine receptor and the ubiquitous S100A1 Ca2+ binding protein. S100A1 did not affect equilibrium [3H]ryanodine binding to purified rabbit skeletal muscle terminal cisternae at 100 microM free [Ca2+]. At nanomolar free [Ca2+], however, S10...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970160w
更新日期：1997-09-23 00:00:00
abstract：:Nonhomologous end joining (NHEJ) is the primary mechanism by which mammalian cells repair DNA double-strand breaks (DSBs). Proteins known to play a role in NHEJ include the DNA-dependent protein kinase catalytic subunit (DNA-PKcs), the Ku 70/Ku 80 heterodimer (Ku), XRCC4, and DNA ligase IV. One of the main roles of th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0524060
更新日期：2006-04-04 00:00:00
abstract：:LTbetaR is a member of the TNF receptor family of proteins. It binds to two different cell surface ligands, LIGHT, a homotypic trimer, and LTalpha1beta2, a heterotypic trimer. We have measured the affinities of the dimeric IgG fusion protein, LTbetaRIgG, and monomeric LTbetaR protein binding to both LIGHT and LTalpha1...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060210+
更新日期：2006-08-22 00:00:00
abstract：:Unoccupied estrogen receptor (ER) can be extracted from tissues by homogenization with a hypotonic buffer, whereas hormone-occupied ER becomes tightly bound to the nuclear pellet and must be extracted with high-salt-containing buffers. The molecular basis for estrogen-induced tight nuclear binding of ER remains an imp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982190k
更新日期：1999-06-01 00:00:00
abstract：:Volumes of lipid dispersions as a function of temperature have been measured for two different kinds of binary mixtures of lecithins, (1) DMPC and DSPC and (2) DMPC and DC20PC. Emphasis was placed on DMPC-rich compositions so as to resolve ambiguities regarding solid-phase immiscibility in DMPC-DSPC mixtures. Special ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00586a032
更新日期：1979-09-18 00:00:00
abstract：:Despite many studies suggesting the role of enolpyruvate as a bound intermediate in the pyruvate kinase reaction, direct evidence for it has been lacking. By use of a combination of chemical trapping and isolation of a derivative, significant amounts of enzyme-bound enolpyruvate have now been demonstrated. The method ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00217a022
更新日期：1991-01-22 00:00:00
abstract：:Bacterial nucleoid-associated proteins (NAPs) are critical to genome integrity and chromosome maintenance. Post-translational modifications of bacterial NAPs appear to function similarly to their better studied mammalian counterparts. The histone-like NAP HupB from Mycobacterium tuberculosis (Mtb) was previously obser...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b01089
更新日期：2018-02-06 00:00:00
abstract：:S-(4-Bromo-2,3-dioxobutyl)glutathione (S-BDB-G), a reactive analogue of glutathione, has been synthesized and characterized by UV spectroscopy and thin-layer chromatography, as well as by bromide and primary amine analysis. Incubation of S-BDB-G (200 microM) with the 4-4 isoenzyme of rat liver glutathione S-transferas...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00111a006
更新日期：1991-11-26 00:00:00
