Abstract:
:The effects of nonylphenol and 3,5-dibutyl-4-hydroxytoluene (BHT) on the activity of the (Ca2(+)-Mg2+)-ATPase of skeletal muscle sarcoplasmic reticulum have been studied. At high concentrations, both inhibit the ATPase activity of the ATPase either in native lipid or in bilayers of dioleoylphosphatidylcholine but, at low concentrations, an increase in ATPase activity is observed, particularly for the ATPase reconstituted into dimyristoleoylphosphatidylcholine. Neither nonylphenol nor BHT binds at the lipid-protein interface of the ATPase. Nonylphenol decreases the effective equilibrium constant for phosphorylation of the ATPase by Pi probably through an increase in the effective rate of dephosphorylation of the phosphorylated ATPase. It also decreases the effective rate of the E2-Ca2E1 transition and increases the effective equilibrium constant E2/E1 for the ATPase. Inhibition of ATPase activity follows from the slowing of the E2-E1 transition despite increases in effective rates for dephosphorylation and for the transport step, Ca2E1P-E2P. Since nonylphenol has been shown to affect equilibrium constants for various steps in the reaction pathway of the ATPase, inhibition of activity of the ATPase cannot follow from effects on the fluidity (viscosity) of the membrane, since fluidity alone cannot affect equilibrium properties of the system.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Michelangeli F,Orlowski S,Champeil P,East JM,Lee AGdoi
10.1021/bi00464a028subject
Has Abstractpub_date
1990-03-27 00:00:00pages
3091-101issue
12eissn
0006-2960issn
1520-4995journal_volume
29pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::In eukaryotes, choline acetyltransferase (ChAT) catalyzes the reversible formation of the neurotransmitter acetylcholine from choline and acetyl-CoA. ChAT belongs to a family of CoA-dependent enzymes that also includes the carnitine acyltransferases CrAT, CrOT, and CPTs. In contrast to CrOT and CPTs that are very acti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1007996
更新日期:2010-07-27 00:00:00
abstract::The relaxin-like factor (RLF) also named insulin-like 3 (INSL3) consists of two polypeptide chains linked by two interchain and one intrachain disulfide bond. RLF binds to its receptor (LGR8 also named RXFP2) through the B chain and initiates transmembrane communication by activating the adenylate cyclase through the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300352x
更新日期:2012-05-22 00:00:00
abstract::Detailed information on room-temperature structure and oxidation state of the Photosystem II (PS II) manganese complex is needed to put mechanistic considerations on solid grounds. Because previously this information had not been available, the tetranuclear manganese complex was investigated by X-ray absorption spectr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9924258
更新日期:2000-06-20 00:00:00
abstract::Six affinity reagents containing chemically reactive groups, either on the phosphate residue at the 5'-end or on the 5'- or 3'-end internucleoside phosphate linkages of the oligothymidylate primers, were used to covalently modify the human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT). After covalent ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00065a015
更新日期:1993-04-13 00:00:00
abstract::A series of antisense oligonucleotides (ASOs) were synthesized and tested to define the best target sites within an RNA transcript of collagen for effective inhibition of expression. The test system consisted of mouse NIH 3T3 fibroblasts that were stably transfected with a human minigene for procollagen I so that the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00202a024
更新日期:1994-09-13 00:00:00
abstract::We compared the kinetics and thermodynamics of rhodamine phalloidin binding to actin purified from rabbit skeletal muscle, Acanthamoeba castellanii, and Saccharomyces cerevisiae in 50 mM KCl, 1 mM MgCl2, and pH 7.0 buffer at 22 degrees C. Filaments of S. cerevisiae actin bind rhodamine phalloidin more weakly than Acan...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961047t
更新日期:1996-11-12 00:00:00
abstract::Comparative studies on the salt titration and the related kinetics for poly(dG-dC) X poly(dG-dC) in pH 7.0 and 3.8 solutions clearly suggest that base protonation facilitates the kinetics of B-Z interconversion although the midpoint for such a transition in acidic solution (2.0-2.1 M NaCl) is only slightly lower than ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00320a041
更新日期:1984-12-04 00:00:00
abstract::The Mn4 complex which is involved in water oxidation in photosystem II (PSII) is known to exhibit two types of EPR signals in the S2 state, one of the five redox states of the enzyme cycle: either a multiline signal (S = 1/2) or a signal at g = 4.1 (S = 3/2 or S= 5/2). The S = 1/2 state can be converted to that respon...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9728710
更新日期:1998-03-24 00:00:00
abstract::In the present report we studied the interaction between the skeletal muscle ryanodine receptor and the ubiquitous S100A1 Ca2+ binding protein. S100A1 did not affect equilibrium [3H]ryanodine binding to purified rabbit skeletal muscle terminal cisternae at 100 microM free [Ca2+]. At nanomolar free [Ca2+], however, S10...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970160w
更新日期:1997-09-23 00:00:00
abstract::The labeling of chloroplast coupling factor 1 by 3'-O-(4-benzoyl)benzoyl-ATP (BzATP) was studied. When the enzyme was incubated with approximately 10 microM BzATP and 6 mM MgCl2 at pH 7.9 for approximately 20 min and passed through two Sephadex G-50 centrifuge columns, three BzATP molecules were bound per coupling fac...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00385a038
更新日期:1987-06-02 00:00:00
abstract::The amino acid sequence of the sex steroid binding protein (SBP) from human plasma has been determined. The SBP subunit consists of a 373-residue polypeptide chain containing two disulfide bonds and three oligosaccharide chains. The sequence was solved primarily by analysis of peptides derived by cleavage at either ly...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00371a048
更新日期:1986-11-18 00:00:00
abstract::Stopped-flow total intensity and anisotropy experiments on single tryptophan containing mutants of yeast phosphoglycerate kinase (PGK) located in either the carboxy-terminal domain (W308 and W333), amino-terminal domain (W48 and W122), or "hinge" region (W194 and W399) were performed. The results obtained for single t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00042a028
更新日期:1995-10-24 00:00:00
abstract::The monoamine transporter of the chromaffin granule membranes can be specifically labeled by the photoaffinity reagent 7-azido-8-[125I]iodoketanserin. The characteristics of the labeled protein have been investigated. Two-dimensional gel electrophoresis of the labeled membranes indicated a MW of about 70,000 and an is...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00122a012
更新日期:1992-02-25 00:00:00
abstract::Cytochromes c require covalent attachment of heme via two thioether bonds at conserved CXXCH motifs, a process accomplished in prokaryotes by eight integral membrane proteins (CcmABCDEFGH), termed System I. Heme is trafficked from inside the cell to outside (via CcmABCD) and chaperoned (holoCcmE) to the cytochrome c s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00427
更新日期:2016-06-07 00:00:00
abstract::The properties of the Fe and Mo sites of the iron-molybdenum cofactor of nitrogenase with respect to binding and activation of N(2) have been studied by molecular mechanics calculations on the local protein environment and by density functional theory (DFT) calculations on subsections of the cofactor. The DFT calculat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi025623z
更新日期:2002-11-26 00:00:00
abstract::Protein-protein interactions such as those between small G proteins and their effector proteins control most cell signaling pathways and thereby govern many cellular processes in both normal and disease states. Each small G protein interacts with several effectors, some shared between similar G proteins and others uni...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701539x
更新日期:2007-12-11 00:00:00
abstract::Cystathionine β-synthase (CBS) catalyzes the first step in the transsulfuration pathway in mammals, i.e., the condensation of serine and homocysteine to produce cystathionine and water. Recently, we have reported a steady-state kinetic analysis of the three hydrogen sulfide (H(2)S)-generating reactions that are cataly...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1010893
更新日期:2011-01-25 00:00:00
abstract::Escherichia coli class Ib ribonucleotide reductase (RNR) converts nucleoside 5'-diphosphates to deoxynucleoside 5'-diphosphates in iron-limited and oxidative stress conditions. We have recently demonstrated in vitro that this RNR is active with both diferric-tyrosyl radical (Fe(III)(2)-Y(•)) and dimanganese(III)-Y(•) ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101881d
更新日期:2011-03-15 00:00:00
abstract::Stopped-flow and laser photolysis methods have been used to investigate the rates of electron-transfer reactions of fully reduced riboflavin and the three oxidation states of Clostridium pasteurianum flavodoxin. Both normal and 7,8-dichloroflavin analogues were studied. Redox reagents included oxygen, ferricyanide, fe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00521a005
更新日期:1981-09-01 00:00:00
abstract::The initial step of infection of blood cells with the human immunodeficiency virus, HIV, is the formation of a complex of the viral envelope protein gp120 and its human receptor CD4. We have examined structural features of recombinant soluble CD4 (sCD4) by chemical modification of arginine residues with hydroxyphenylg...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011626k
更新日期:2002-02-12 00:00:00
abstract::The kinetics and mechanism of cleavage of DNA by the insulin-mimetic peroxo-vanadate NH4[VO(O2)2(phen)], pV, are described. In the presence of low energy UV radiation or biologically common reducing agents, pV decomposes into the monomer, dimer, and tetramer of vanadate and an uncharacterized compound of V4+ as shown ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9606253
更新日期:1996-09-24 00:00:00
abstract::From a series of 15N-resolved 3D ROESY-HMQC and 13C-resolved 3D NOESY-HMQC spectra of the FK506 binding protein (FKBP)/ascomycin complex in H2O, the locations of three tightly bound water molecules were identified. These waters are all buried within the interior of the complex and form an integral part of its structur...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00061a004
更新日期:1993-03-16 00:00:00
abstract::Translational initiation factor IF-2 is involved in a multistep pathway leading to the synthesis of the first peptide bond. IF-2 is a guanine nucleotide binding protein (G-protein) and catalyzes GTP hydrolysis in the presence of ribosomes. According to sequence homologies with other G-proteins, particularly EF-Tu, a t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00493a031
更新日期:1990-10-16 00:00:00
abstract::Whether hydrogen bonds between side chains are energetically significant in proteins and peptides has been controversial. A method is given here for measuring these interactions in peptide helices by comparing the helix contents of peptides with 1, 2, or 3 interactions. Results are given for the glutamine--aspartate (...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00041a001
更新日期:1995-10-17 00:00:00
abstract::We have studied the properties and structures of a series of Escherichia coli RNA polymerase ternary complexes formed during the initial steps of RNA chain initiation and elongation. Five different templates were used that contained the bacteriophage T7 A1 promoter or the E. coli Tac or the lac UV5 promoter, as well a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00445a045
更新日期:1989-09-19 00:00:00
abstract::A grand canonical formalism is developed to combine discrete simulations for chemically distinct species in equilibrium. Each simulation is based on a perturbed funneled landscape. The formalism is illustrated using the alkaline-induced transitions of cytochrome c as observed by FTIR spectroscopy and with various othe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi802293m
更新日期:2009-03-24 00:00:00
abstract::AhpF, the flavoprotein reductase component of the Salmonella typhimurium alkyl hydroperoxide reductase system, catalyzes the reduction of an intersubunit disulfide bond in the peroxidatic active site of the system's other component, AhpC, a member of the peroxiredoxin family. Previous studies have shown that AhpF can ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002766h
更新日期:2001-04-03 00:00:00
abstract::G protein-coupled receptor (GPCR) kinases (GRKs) instigate the desensitization of activated GPCRs via phosphorylation that promotes interaction with arrestins, thereby preventing the interaction of GPCRs with heterotrimeric G proteins. A current proposed model of GRK1 activation involves the binding of activated rhodo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300295y
更新日期:2012-04-24 00:00:00
abstract::We have recently reported that the Na pump alpha-subunit has cytosolic-oriented oligosaccharides which were sensitive to cleavage by an enzyme specific for hydrolysis of N-linked glycans [Pedemonte et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 9789-9793]. We now describe experiments that characterize the saccharides...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00158a008
更新日期:1992-11-03 00:00:00
abstract::Oligonucleotide-directed triple helix formation normally requires a long tract of oligopyrimidine.oligopurine sequence. This limitation can be partially overcome by alternate-strand triple helix (or switch triple helix) formation which enables recognition of alternating oligopurine/oligopyrimidine sequences. The prese...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980618+
更新日期:1998-09-22 00:00:00