Measuring the strength of side-chain hydrogen bonds in peptide helices: the Gln.Asp (i, i + 4) interaction.

abstract：:Substitution of Co(II) for the catalytic site Zn(II) of horse liver alcohol dehydrogenase (LADH) yields an active enzyme derivative, CoIIE, with characteristic Co(II) charge-transfer and d-d electronic transitions that are sensitive to the events which take place during catalysis [Koerber, S. C., MacGibbon, A. K. H., ...

journal_title：Biochemistry

authors： Sartorius C,Gerber M,Zeppezauer M,Dunn MF

更新日期：1987-02-10 00:00:00

abstract：:Two nucleoside diphosphate analogs, 3'-C-methyl-ADP and 3'-C-methyl-UDP, have been tested as substrate and/or allosteric effectors using the adenosylcobalamin-dependent ribonucleoside diphosphate reductase of Corynebacterium nephridii. Neither analog was a substrate for the reductase. However, they did function as all...

journal_title：Biochemistry

authors： Ong SP,Nelson LS,Hogenkamp HP

更新日期：1992-11-17 00:00:00

abstract：:Previous studies have established that the mechanism of species-specific sponge cell reaggregation in vitro involves at least three components: a species-specific aggregation factor, its cell surface receptors, and Ca2+ ions. Here we report about the binding of Microciona 125I-labeled aggregation factor to cells and t...

journal_title：Biochemistry

authors： Jumblatt JE,Schlup V,Burger MM

更新日期：1980-03-04 00:00:00

abstract：:Proteins from the enhanced intracellular survival (Eis) family are versatile acetyltransferases that acetylate amines at multiple positions of several aminoglycosides (AGs). Their upregulation confers drug resistance. Homologues of Eis are present in diverse bacteria, including many pathogens. Eis from Mycobacterium t...

journal_title：Biochemistry

authors： Green KD,Biswas T,Chang C,Wu R,Chen W,Janes BK,Chalupska D,Gornicki P,Hanna PC,Tsodikov OV,Joachimiak A,Garneau-Tsodikova S

更新日期：2015-05-26 00:00:00

abstract：:The mechanisms of interaction of the recombinant N-terminal portion of bactericidal/permeability-increasing protein, rBPI21, with various planar asymmetric and symmetric bilayer membranes, including the lipid matrix of the outer membrane of Gram-negative bacteria, were investigated via electrical measurements. For the...

journal_title：Biochemistry

authors： Wiese A,Brandenburg K,Carroll SF,Rietschel ET,Seydel U

更新日期：1997-08-19 00:00:00

abstract：:Trimethylamine dehydrogenase (TMADH) from the bacterium Methylophilus methylotrophus (sp. W(3)A(1)) and its C30A mutant were inactivated with three known inactivators of monoamine oxidase, namely, phenylhydrazine, N-cyclopropyl-alpha-methylbenzylamine, and 1-phenylcyclopropylamine. All three compounds irreversibly ina...

journal_title：Biochemistry

authors： Mitchell DJ,Nikolic D,Jang MH,van Breemen RB,Hille R,Silverman RB

更新日期：2001-07-24 00:00:00

abstract：:The interaction of poly(N epsilon, N epsilon, N epsilon-trimethyl-L-lysine) ([Lys(Me3)]n) and poly(N delta, N delta, N delta-trimethyl-L-ornithine) ([Orn(Me3)]n) with polynucleotides was studied by thermal denaturation, viscosity, and dissociation by salt. The methylated polymers decrease the viscosity of DNA in propo...

journal_title：Biochemistry

authors： Granados EN,Bello J

更新日期：1980-07-08 00:00:00

abstract：:The serotonin transporter (SERT) is a member of a highly homologous family of proteins responsible for the reuptake of biogenic amines from the synaptic cleft. We took advantage of native restriction sites in SERT to construct a chimeric transporter containing a small (34 amino acid) region of the norepinephrine trans...

journal_title：Biochemistry

authors： Stephan MM,Chen MA,Penado KM,Rudnick G

更新日期：1997-02-11 00:00:00

abstract：:The heme binding sites of rabbit histidine-rich glycoprotein (HRG), 94 kDa, were studied with rose bengal (RB), a fluorescein derivative that associates with histidine residues. Difference absorbance spectra indicate that HRG binds RB at two thermodynamically preferred sites (Kd approximately 2 microM) that are spectr...

journal_title：Biochemistry

authors： Burch MK,Morgan WT

更新日期：1985-10-08 00:00:00

abstract：:There is considerable evidence that long-range interactions stabilize residual protein structure under denaturing conditions. However, evaluation of the effect of a specific contact on structure in the denatured state has been difficult. Iso-1-cytochrome c variants with a Lys54 → His mutation form a particularly stabl...

journal_title：Biochemistry

authors： Danielson TA,Stine JM,Dar TA,Briknarova K,Bowler BE

更新日期：2017-12-26 00:00:00

abstract：:The flavin-dependent quiescin-sulfhydryl oxidase (QSOX) inserts disulfide bridges into unfolded reduced proteins with the reduction of molecular oxygen to form hydrogen peroxide. This work investigates how QSOX and protein disulfide isomerase (PDI) cooperate in vitro to generate native pairings in two unfolded reduced...

journal_title：Biochemistry

authors： Rancy PC,Thorpe C

更新日期：2008-11-18 00:00:00

abstract：:Mycoplasmas are the smallest known microorganisms, with drastically reduced genome sizes. One of the essential biochemical pathways lost in mycoplasmas is methylation-mediated DNA repair (MMR), which is responsible for correction of base substitutions, insertions, and deletions in both bacteria and higher organisms. W...

journal_title：Biochemistry

authors： Kamashev D,Oberto J,Serebryakova M,Gorbachev A,Zhukova Y,Levitskii S,Mazur AK,Govorun V

更新日期：2011-10-11 00:00:00

abstract：:Matrix metalloproteinases (MMPs) are a family of enzymes that are up-regulated in many diseases, including osteoarthritis (OA) and rheumatoid arthritis (RA). Here we report on a novel technique that can be used to simultaneously measure activity levels for a panel of enzymes, such as the MMPs. The technique, termed th...

journal_title：Biochemistry

authors： Rasmussen FH,Yeung N,Kiefer L,Murphy G,Lopez-Otin C,Vitek MP,Moss ML

更新日期：2004-03-23 00:00:00

abstract：:Calcium trapped within sonicated and resealed bovine rod outer segment disks is released upon light exposure with a stoichiometry of 0.75 +/- 0.05 calcium for each rhodopsin bleached. The amount of calcium liberated is proportional to the amount of bleaching in the range of 20 to 100% bleaching and is relatively insen...

journal_title：Biochemistry

authors： Smith HG Jr,Fager RS,Litman RJ

更新日期：1977-04-05 00:00:00

abstract：:The metabolism of iron-sulfur ([Fe-S]) clusters requires a complex set of machinery that is still being defined. Mutants of Salmonella enterica lacking apbC have nutritional and biochemical properties indicative of defects in [Fe-S] cluster metabolism. ApbC is a 40.8 kDa homodimeric ATPase and as purified contains lit...

journal_title：Biochemistry

authors： Boyd JM,Pierik AJ,Netz DJ,Lill R,Downs DM

更新日期：2008-08-05 00:00:00

abstract：:The X-ray structures of the ligand free (apo) and the Mg(2+)*o-succinylbenzoate (OSB) product complex of o-succinylbenzoate synthase (OSBS) from Escherichia coli have been solved to 1.65 and 1.77 A resolution, respectively. The structure of apo OSBS was solved by multiple isomorphous replacement in space group P2(1)2(...

journal_title：Biochemistry

authors： Thompson TB,Garrett JB,Taylor EA,Meganathan R,Gerlt JA,Rayment I

更新日期：2000-09-05 00:00:00

abstract：:Heme-regulated eIF2alpha kinase [heme-regulated inhibitor (HRI)] plays a critical role in the regulation of protein synthesis by heme iron. The kinase active site is located in the C-terminal domain, whereas the N-terminal domain is suggested to regulate catalysis in response to heme binding. Here, we found that the r...

journal_title：Biochemistry

authors： Miksanova M,Igarashi J,Minami M,Sagami I,Yamauchi S,Kurokawa H,Shimizu T

更新日期：2006-08-15 00:00:00

abstract：:Cytochrome P450 3A4 (CYP3A4) inhibitors ritonavir and cobicistat, currently administered to HIV patients as pharmacoenhancers, were designed on the basis of the chemical structure/activity relationships rather than the CYP3A4 crystal structure. To better understand the structural basis for CYP3A4 inhibition and the li...

journal_title：Biochemistry

authors： Sevrioukova IF,Poulos TL

更新日期：2013-07-02 00:00:00

abstract：:l-Aspartate oxidase, encoded by the nadB gene, is the first enzyme in the de novo synthesis of NAD+ in bacteria. This FAD-dependent enzyme catalyzes the oxidation of l-aspartate to generate iminoaspartate and reduced flavin. Distinct from most amino acid oxidases, it can use either molecular oxygen or fumarate to reox...

journal_title：Biochemistry

authors： Chow C,Hegde S,Blanchard JS

更新日期：2017-08-08 00:00:00

abstract：:Standard preparations of Escherichia coli RNA polymerase harbor a 70 kDa protein with NTPase (beta-gamma cleavage) activity that is not a recognized polymerase subunit. The NTPase activity of this component, before and after separation from the polymerase, is strongly dependent on the presence of DNA; single-stranded ...

journal_title：Biochemistry

authors： Butzow JJ,Garland C,Van Lee L,Eichhorn GL

更新日期：1997-12-02 00:00:00

abstract：:High signal-to-noise Fourier transform infrared (FTIR) spectra of the 5-hydroxytryptamine (serotonin) receptor (5-HT(3)R) and the nicotinic acetylcholine receptor (nAChR) were obtained by microscope FTIR spectroscopy using micrometer-sized, fully hydrated protein films. Because this novel procedure requires only nanog...

journal_title：Biochemistry

authors： Rigler P,Ulrich WP,Hovius R,Ilegems E,Pick H,Vogel H

更新日期：2003-12-02 00:00:00

abstract：:Photoreceptor guanylyl cyclase (ROS-GC), converting GTP into cGMP and pyrophosphate, is a key enzyme in the regulation of the visual transduction cascade. ROS-GC requires GC-activating proteins (GCAPs) and low free [Ca] for full activity. We found that when choline or potassium were the major cations present, light ca...

journal_title：Biochemistry

authors： Wolbring G,Baehr W,Palczewski K,Schnetkamp PP

更新日期：1999-03-02 00:00:00

abstract：:In an attempt to understand the mechanism by which transcription factors compete with histone octamers for cognate binding sites in chromatin, the effect of the histone binding protein nucleoplasmin on the binding of TFIIIA to nucleosomal 5S rRNA genes was tested. In this study, it was shown that, despite the previous...

journal_title：Biochemistry

authors： Howe L,Itoh T,Katagiri C,Ausio J

更新日期：1998-02-03 00:00:00

abstract：:Antisense oligodeoxyribonucleoside methylphosphonates targeted against various regions of mRNA or precursor mRNA are selective inhibitors of mRNA expression both in cell-free systems and in cells in culture. The efficiency with which methylphosphonate oligomers interact with mRNA, and thus inhibit translation, can be ...

journal_title：Biochemistry

authors： Kean JM,Murakami A,Blake KR,Cushman CD,Miller PS

更新日期：1988-12-27 00:00:00

abstract：:Significant concentrations (1-10 mM) of nickel(II) were found in solution after incubation of the potent carcinogen nickel subsulfide in 0.05 M Tris-HCl, pH 7.4, solutions containing DNA, rat liver microsomes, and NADPH. The presence of NADPH decreased the rate of solubilization of nickel subsulfide. The solubilized n...

journal_title：Biochemistry

authors： Lee JE,Ciccarelli RB,Jennette KW

更新日期：1982-02-16 00:00:00

abstract：:The flexibility of individual amino acid side chains of pancreatic phospholipase A2 in aqueous and micellar solutions was studied with deuterium nuclear magnetic resonance (2H NMR). Bovine pancreatic phospholipase A2 was selectively deuterated at the aromatic ring systems of Trp-3 and Phe-5 and porcine pancreatic phos...

journal_title：Biochemistry

authors： Allegrini PR,van Scharrenburg GJ,Slotboom AJ,de Haas GH,Seelig J

更新日期：1985-06-18 00:00:00

abstract：:Thirty single cysteine substitution mutants of T4 lysozyme have been prepared and spin-labeled with a sulfhydryl-specific nitroxide reagent in order to systematically investigate the relationship between nitroxide side-chain mobility and protein structure. The perturbation caused by replacement of a native residue wit...

journal_title：Biochemistry

authors： Mchaourab HS,Lietzow MA,Hideg K,Hubbell WL

更新日期：1996-06-18 00:00:00

abstract：:In aqueous glycerol solutions, the rate constant for the formation of compound I of horseradish peroxidase from hydrogen peroxide is independent of the viscosity. However, whem m-chloroperbenzoic acid is used to form compound I, the rate is viscosity dependent, indicating that this reaction is diffusion controlled. Th...

journal_title：Biochemistry

authors： Dunford HB,Hewson WD

更新日期：1977-06-28 00:00:00

abstract：:Both metal and flavin-dependent sulfhydryl oxidases catalyze the net generation of disulfide bonds with the reduction of oxygen to hydrogen peroxide. The first mammalian sulfhydryl oxidase to be described was an iron-dependent enzyme isolated from bovine milk whey (Janolino, V.G., and Swaisgood, H.E. (1975) J. Biol. C...

journal_title：Biochemistry

authors： Jaje J,Wolcott HN,Fadugba O,Cripps D,Yang AJ,Mather IH,Thorpe C

更新日期：2007-11-13 00:00:00

abstract：:DNA sequences containing homopurine d(G1-3A)n tracts are known to be capable of adopting non-B-DNA conformations. The structural polymorphism of these sequences is a direct consequence of the structural properties of the homopurine d(G1-3A)n tracts. Depending on the conditions, d(GA)n DNA sequences can form antiparall...

journal_title：Biochemistry

authors： Huertas D,Azorín F

更新日期：1996-10-08 00:00:00