Abstract:
:Antisense oligodeoxyribonucleoside methylphosphonates targeted against various regions of mRNA or precursor mRNA are selective inhibitors of mRNA expression both in cell-free systems and in cells in culture. The efficiency with which methylphosphonate oligomers interact with mRNA, and thus inhibit translation, can be considerably increased by introducing photoactivatable psoralen derivatives capable of cross-linking with the mRNA. Oligonucleoside methylphosphonates complementary to coding regions of rabbit alpha- or beta-globin mRNA were derivatized with 4'-(aminoalkyl)-4,5',8-trimethylpsoralens by attaching the psoralen group to the 5' end of the oligomer via a nuclease-resistant phosphoramidate linkage. The distance between the psoralen group and the 5' end of the oligomer can be adjusted by changing the number of methylene groups in the aminoalkyl linker arm. The psoralen-derivatized oligomers specifically cross-link to their complementary sequences on the targeted mRNA. For example, an oligomer complementary to nucleotides 56-67 of alpha-globin mRNA specifically cross-linked to alpha-globin mRNA upon irradiation of a solution of the oligomer and rabbit globin mRNA at 4 degrees C. Oligomers derivatized with 4'-[[N-(2-amino-ethyl)amino]methyl]-4,5',8-trimethylpsoralen gave the highest extent of cross-linking to mRNA. The extent of cross-linking was also determined by the chain length of the oligomer and the structure of the oligomer binding site. Oligomers complementary to regions of mRNA that are sensitive to hydrolysis by single-strand-specific nucleases cross-linked to an approximately 10-30-fold greater extent than oligomers complementary to regions that are insensitive to nuclease hydrolysis.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Kean JM,Murakami A,Blake KR,Cushman CD,Miller PSdoi
10.1021/bi00426a008subject
Has Abstractpub_date
1988-12-27 00:00:00pages
9113-21issue
26eissn
0006-2960issn
1520-4995journal_volume
27pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::This study was undertaken to identify the alpha-helical domains of human apoE that mediate cellular cholesterol efflux and HDL assembly via ATP-binding cassette transporter A1 (ABCA1). The C-terminal (CT) domain (residues 222-299) of apoE was found to stimulate ABCA1-dependent cholesterol efflux in a manner similar to...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi602407r
更新日期:2007-03-13 00:00:00
abstract::The side chains of histidine and aspartate residues form a hydrogen bond in the active sites of many enzymes. In serine proteases, the His...Asp hydrogen bond of the catalytic triad is known to contribute greatly to catalysis, perhaps via the formation of a low-barrier hydrogen bond. In bovine pancreatic ribonuclease ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972766q
更新日期:1998-06-23 00:00:00
abstract::Detailed 31P NMR measurements have been conducted on pig platelet dense granules and aqueous mixtures of ATP, ADP, MgCl2, and 5HT. The resonance line widths of the dense granule nucleotides were temperature independent above approximately 30 degrees C; below this temperature they exhibit a strong temperature dependenc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00298a004
更新日期:1984-01-31 00:00:00
abstract::The role of pyridoxal 5'-phosphate (PLP) in the radical-mediated amino group migration catalyzed by lysine 2,3-aminomutase from Clostridia SB4 has been investigated by electron spin echo envelope modulation (ESEEM) spectroscopy. This pulsed electron paramagnetic resonance (EPR) method was used to estimate the distance...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00031a033
更新日期:1995-08-08 00:00:00
abstract::In this study, we have investigated the influence of regions outside the DNA-binding domain of the human glucocorticoid receptor on high-affinity DNA binding. We find that the DNA-binding domain shows a 10-fold lower affinity for a palindromic DNA-binding site than the intact receptor. The N-terminal part of the recep...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00152a047
更新日期:1992-09-22 00:00:00
abstract::The kinetics of human polymerase beta (pol beta) binding to the single-stranded DNA, in the (pol beta)(16) and (pol beta)(5) binding modes, that differ in the number of occluded nucleotide residues in the protein-DNA complexes, have been examined, using the fluorescence stopped-flow technique. This is the first determ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011173j
更新日期:2001-10-02 00:00:00
abstract::Vitamin K dependent carboxylation of an exogenous peptide substrate and endogenous protein substrates, vitamin K epoxidation, and reduction of vitamin K epoxide were measured in subcellular fractions from rat liver. The rough microsomal fraction was highly enriched in all four activities; lower levels were found in sm...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00547a019
更新日期:1980-03-18 00:00:00
abstract::Centrioles are essential components of the animal centrosome and play crucial roles in the formation of cilia and flagella. They are cylindrical structures composed of nine triplet microtubules organized around a central cartwheel. Recent studies have identified spindle assembly abnormal protein SAS-6 as a critical co...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00978
更新日期:2015-10-20 00:00:00
abstract::Under mildly acidic conditions, annexin 12 (ANX) inserts into lipid membranes to form a transbilayer pore [Langen, R., et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 14060]. In this study, we have addressed the question of the oligomeric state of ANX in this transbilayer conformation by means of Forster-type resonanc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047805u
更新日期:2005-03-08 00:00:00
abstract::Plasma kallikrein (Fletcher factor) is a hepatic serine proteinase that participated in the early phase of blood coagulation. From two genomic libraries, we succeeded to isolate four overlapping clones representing the entire rat plasma kallikrein gene. Using selective DNA sequencing, polymerase chain reactions, and r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00220a027
更新日期:1991-02-12 00:00:00
abstract::Because oxygen and selenium are in the same group (Family VI) in the periodic table, the site-specific mutagenesis at the atomic level by replacing RNA oxygen with selenium can provide insights on the structure and function of catalytic RNAs. We report here the first Se-derivatized ribozymes transcribed with all nucle...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060455m
更新日期:2006-07-25 00:00:00
abstract::BtuB is a TonB-dependent transport protein that binds and carries vitamin B(12) across the outer membrane of Gram negative bacteria such as Escherichia coli. Previous work has demonstrated that the Ton box, a highly conserved segment near the N-terminus of the protein, undergoes an order-to-disorder transition upon th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061051x
更新日期:2006-09-12 00:00:00
abstract::Uronate isomerase (URI) catalyzes the reversible isomerization of D-glucuronate to D-fructuronate and of D-galacturonate to D-tagaturonate. URI is a member of the amidohydrolase superfamily (AHS), a highly divergent group of enzymes that catalyze primarily hydrolytic reactions. The chemical mechanism and active site s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901046x
更新日期:2009-09-22 00:00:00
abstract::The crystal structures of the guanosine 5'-monophosphate (GMP) and inosine 5'-monophosphate (IMP) complexes of Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase (HGPRT) have been determined at 1.65 and 1.90 A resolution. These complexes, which crystallize in space groups P2(1) (a = 65.45 A, b = 90.84 A,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990507q
更新日期:1999-11-02 00:00:00
abstract::Previous studies have established that the mechanism of species-specific sponge cell reaggregation in vitro involves at least three components: a species-specific aggregation factor, its cell surface receptors, and Ca2+ ions. Here we report about the binding of Microciona 125I-labeled aggregation factor to cells and t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00546a032
更新日期:1980-03-04 00:00:00
abstract::The structure and folding of a novel human insulin mutant, [Thr(B27) --> Pro, Pro(B28) --> Thr]insulin (PT insulin), in aqueous solution and in mixtures of water and 2,2,2-trifluoroethanol (TFE) have been studied by NMR spectroscopy. It was found that PT insulin has a highly flexible structure in pure water and is pre...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0108150
更新日期:2001-09-04 00:00:00
abstract::Group VIA phospholipase A(2) (iPLA(2)beta) is expressed in phagocytes, vascular cells, pancreatic islet beta-cells, neurons, and other cells and plays roles in transcriptional regulation, cell proliferation, apoptosis, secretion, and other events. A bromoenol lactone (BEL) suicide substrate used to study iPLA(2)beta f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060502a
更新日期:2006-05-23 00:00:00
abstract::It is well-known that the rapid flow of water into and out of cells is controlled by membrane proteins called aquaporins (AQPs). However, the mechanisms that allow cells to quickly respond to a changing osmotic environment are less well established. Using GFP-AQP fusion proteins expressed in HEK293 cells, we demonstra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi902068b
更新日期:2010-02-09 00:00:00
abstract::The phosphoryl-binding elements in the GDP-binding domain of elongation factor Tu were studied by heteronuclear proton observe methods. Five proton resonances were found below 10.5 ppm. Two of these were assigned to the amide groups of Lys 24 and Gly 83. These are conserved residues in each of the consensus sequences....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00109a010
更新日期:1991-11-12 00:00:00
abstract::An important subject for elucidating membrane protein (MP) folding is how transmembrane helices (TMHs) insert into and dissociate from membranes. We investigated helix dissociation kinetics and insertion topology by means of intervesicular transfer of the fluorophore-labeled completely hydrophobic model transmembrane ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026191v
更新日期:2002-10-15 00:00:00
abstract::Abortive transcription initiation can be rate-limiting for promoter escape and therefore represents a barrier to productive gene expression. The mechanism for abortive initiation is unknown, but the amount of abortive transcript is known to vary with the composition of the initial transcribed sequence (ITS). Here, we ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00272
更新日期:2015-07-21 00:00:00
abstract::A wide range of two-dimensional 1H NMR experiments have been used to completely assign the 500-MHz 1H NMR spectrum of recombinant Ca2+-saturated bovine calbindin D9k (76 amino acids, Mr = 8500). In solution, calbindin D9k exists as an equilibrium mixture of isoforms with trans (75%) and cis (25%) isomers of the peptid...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00443a043
更新日期:1989-08-22 00:00:00
abstract::The kinetic and equilibrium CO binding properties of hemoglobin (Hb) M Iwate (alpha2 87 His leads to Tyr beta2) have been investigated. The results show that the alpha(Met) beta2 (CO) tetramer of this protein has a low affinity for CO, as indicated by the stopped-flow and flash-photolysis kinetic, as well as the CO bi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00669a022
更新日期:1976-11-30 00:00:00
abstract::Studies of intragenomic homologues in bacterial genomes can provide valuable insights into functional divergence. Three GTP cyclohydrolase II homologues in the Streptomyces coelicolor genome have been shown to catalyze two related but distinct reactions [Spoonamore, J. E., Dahlgran, A. L., Jacobsen, N. E., and Bandari...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi702263z
更新日期:2008-02-26 00:00:00
abstract::Charybdotoxin (ChTX) and iberiotoxin (IbTX) are highly charged peptidyl toxins which exhibit 68% sequence identity and share a similar three-dimensional structure. Despite these structural similarities, IbTX and ChTX differ in their selectivity for two types of potassium channels; large conductance calcium-activated p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00060a030
更新日期:1993-03-09 00:00:00
abstract::The effects of pressure, up to 5 kbar, on multilamellar vesicles of 1,2-dipalmitoyl-sn-phosphatidylcholine perdeuterated in the acyl chains (DPPC-d62) were examined by using high-pressure NMR techniques. A deuterium probe was built, and the quadrupole splitting was measured against pressure at various temperatures. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00227a022
更新日期:1991-04-02 00:00:00
abstract::The low level of enzymatic activity of certain alpha 2-macroglobulin-proteinase complexes could be important to the function of factor VIII/von Willebrand glycoprotein since it is especially sensitive to proteolytic cleavage. To test this possibility, complexes of alpha 2-macroglobulin with plasmin, trypsin, and throm...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00275a018
更新日期:1983-03-15 00:00:00
abstract::Taurine/alpha-ketoglutarate (alphaKG) dioxygenase (TauD), an archetype alphaKG-dependent hydroxylase, is a non-heme mononuclear Fe(II) enzyme that couples the oxidative decarboxylation of alphaKG with the conversion of taurine to aminoacetaldehyde and sulfite. The crystal structure of taurine-alphaKG-Fe(II)TauD is kno...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048746n
更新日期:2005-03-15 00:00:00
abstract::The kinetic parameters (Km and Vmax) of sugar-modified analogues of inosine and guanosine have been determined with human erythrocytic purine nucleoside phosphorylase (PNP). Steric alterations at the 2' and 3' positions greatly lessened or abolished substrate activity. However, the 5'-deoxy- and 2',5'-dideoxy-beta-D-r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00542a016
更新日期:1980-01-08 00:00:00
abstract::The systems of tRNA glycylation belong to the most complex aminoacylation systems since neither the oligomeric structure of glycyl-tRNA synthetases (GlyRS) nor the discriminator bases in tRNAGly are conserved in the phylae. To better understand the structure-function relationship in glycylation systems of various orig...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991392t
更新日期:1999-10-05 00:00:00