Abstract：
:The effects of pressure, up to 5 kbar, on multilamellar vesicles of 1,2-dipalmitoyl-sn-phosphatidylcholine perdeuterated in the acyl chains (DPPC-d62) were examined by using high-pressure NMR techniques. A deuterium probe was built, and the quadrupole splitting was measured against pressure at various temperatures. The experiments were performed on pure lipid bilayers in the liquid-crystalline state and on bilayers in the liquid-crystalline state containing the local anesthetic tetracaine. The results show that the order parameter of all segments of the acyl chains increases with pressure in the liquid-crystalline state. The more highly ordered regions of the chains are affected slightly more than the regions near the methyl ends. The addition of tetracaine increases the disorder of the chains, and pressure reverses the effect of anesthetic on the lipid as seen by the reversal of the changes in line shape and the measured order parameter.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Driscoll DA,Samarasinghe S,Adamy S,Jonas J,Jonas Adoi
10.1021/bi00227a022subject
Has Abstractpub_date
1991-04-02 00:00:00pages
3322-7issue
13eissn
0006-2960issn
1520-4995journal_volume
30pub_type
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