E2P phosphoforms of Na,K-ATPase. I. Comparison of phosphointermediates formed from ATP and Pi by their reactivity toward hydroxylamine and vanadate.

abstract：:Isotope exchange studies show that beef liver fructokinase has a random kinetic mechanism in which release of fructose from the enzyme is slower than that catalytic reaction. The stickiness of fructose in the presence of MgATP is confirmed by isotope partition studies, which show it to be released 0.53 times as fast a...

journal_title：Biochemistry

authors： Raushel FM,Cleland WW

更新日期：1977-05-17 00:00:00

abstract：:7,8-diaminopelargonic acid (DAPA) synthase (EC 2.6.1.62) is a pyridoxal phosphate (PLP)-dependent transaminase that catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form DAPA in the antepenultimate step in the biosynthesis of biotin. The wild...

journal_title：Biochemistry

authors： Eliot AC,Sandmark J,Schneider G,Kirsch JF

更新日期：2002-10-22 00:00:00

abstract：:Although point mutations usually lead to minor localized changes in protein structure, replacement of conserved Pro-76 with Gly in iso-2-cytochrome c induces a major conformational change. The change in structure results from mutation-induced depression of the pK for transition to an alkaline conformation with altered...

journal_title：Biochemistry

authors： Nall BT,Zuniga EH,White TB,Wood LC,Ramdas L

更新日期：1989-12-12 00:00:00

abstract：:Chemical deglycosylation of gonadotropins with hydrogen fluoride (HF) has facilitated the investigation of the structure-function relationship of the individual peptide and oligosaccharide moieties in the mechanism of hormone action. These studies have dealt almost exclusively with lutropin or human choriogonadotropin...

journal_title：Biochemistry

authors： Calvo FO,Keutmann HT,Bergert ER,Ryan RJ

更新日期：1986-07-01 00:00:00

abstract：:The antimycotic drug clotrimazole inhibits the function of the gastric H,K-ATPase in a manner similar to that observed for the Na,K-ATPase. Because of the high hydrophobicity of the compound, the interaction between clotrimazole and the ion pump occurs at the membrane domain in the apolar core of the membrane. The enz...

journal_title：Biochemistry

authors： Witzke A,Lindner K,Munson K,Apell HJ

更新日期：2010-06-01 00:00:00

abstract：:In this study, we have investigated the effect of hydrophobic mismatch between the thickness of the membrane and a transmembrane segment of a protein that directly inserts into the membrane bilayer. For this purpose we used mutants of the single-spanning Pf3 coat protein that can spontaneously insert into Escherichia ...

journal_title：Biochemistry

authors： Ridder AN,van de Hoef W,Stam J,Kuhn A,de Kruijff B,Killian JA

更新日期：2002-04-16 00:00:00

abstract：:Cystathionine gamma-synthase (CGS) is a pyridoxal phosphate-dependent enzyme that catalyzes a gamma-replacement reaction, in which the succinyl group of an O-succinyl-L-homoserine (L-OSHS) is displaced by the thiol of L-cysteine to form L-cystathionine, in the first step of the bacterial transsulfuration pathway. The ...

journal_title：Biochemistry

authors： Aitken SM,Kim DH,Kirsch JF

更新日期：2003-09-30 00:00:00

abstract：:Pseudomonas aeruginosa PAO1 encodes two outer membrane receptors, PhuR (Pseudomonas heme uptake) and HasR (heme assimilation system). The HasR receptor acquires heme through interaction with a secreted hemophore, HasAp. The non-hemophore-dependent PhuR is encoded along with proteins required for heme translocation int...

journal_title：Biochemistry

authors： Smith AD,Modi AR,Sun S,Dawson JH,Wilks A

更新日期：2015-04-28 00:00:00

abstract：:The structure formed from the DNA oligonucleotide d(G4T4G4) (Oxy-1.5), which contains the Oxytricha telomere repeat T4G4, has been investigated by two-dimensional 1H and 31P NMR spectroscopy. Sequence-specific assignments have been obtained for the 1H and 31P resonances, using a combination of methods including compar...

journal_title：Biochemistry

authors： Smith FW,Feigon J

更新日期：1993-08-24 00:00:00

abstract：:RNA covalently linked to double-stranded RNA (dsRNA) is preferentially degraded in extracts of interferon-treated HeLa cells [Nilsen, T. W., & Baglioni, C. (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 2600-2604]. The size of the dsRNA required for this preferential degradation has been determined by annealing poly(I) of k...

journal_title：Biochemistry

authors： Nilsen TW,Weissman SG,Baglioni C

更新日期：1980-11-25 00:00:00

abstract：:Peptide nucleic acids (PNAs) are nonionic DNA/RNA mimics that can recognize complementary sequences by Watson-Crick base pairing. The neutral PNA backbone facilitates the recognition of duplex DNA by strand invasion, suggesting that antigene PNAs (agPNAs) can be important tools for exploring the structure and function...

journal_title：Biochemistry

authors： Hu J,Corey DR

更新日期：2007-06-26 00:00:00

abstract：:The proton nuclear magnetic resonance spectrum of human adult deoxyhemoglobin in D2O in the region from 6 to 20 ppm downfield from the proton resonance of residual water shows a number of hyperfine shifted proton resonances that are due to groups on or near the alpha and beta hemes. The sensitivity of these resonances...

journal_title：Biochemistry

authors： Viggiano G,Ho NT,Ho C

更新日期：1979-11-13 00:00:00

abstract：:Infrared spectroscopy has been used to monitor residual ordered structure in the denatured state of wild-type and two mutants of iso-1-cytochrome c. The technique used involves a careful digital subtraction procedure that removes spectral contributions from buffer, water vapor, and the denaturant guanidine hydrochlori...

journal_title：Biochemistry

authors： Bowler BE,Dong A,Caughey WS

更新日期：1994-03-08 00:00:00

abstract：:S-Glutathionylation is a redox-regulated modification that uncouples endothelial nitric oxide synthase (eNOS), switching its function from nitric oxide (NO) synthesis to (•)O2(-) generation, and serves to regulate vascular function. While in vitro or in vivo eNOS S-glutathionylation with modification of Cys689 and Cys...

journal_title：Biochemistry

authors： Chen CA,De Pascali F,Basye A,Hemann C,Zweier JL

更新日期：2013-09-24 00:00:00

abstract：:Site-directed mutagenesis has been used to construct three recombinant mutant hemoglobins (rHbs), rHb(beta L105W), rHb(alpha D94A/betaL105W), and rHb(alpha D94A). rHb(beta L105W) is designed to form a new hydrogen bond from beta 105Trp to alpha 94Asp in the alpha(1)beta(2) subunit interface to lower the oxygen binding...

journal_title：Biochemistry

authors： Fang TY,Simplaceanu V,Tsai CH,Ho NT,Ho C

更新日期：2000-11-14 00:00:00

abstract：:DBA/2 mouse spleen cells were stimulated in vitro by (a) alloantigen (mitomycin-treated CBA/J splenocytes), (b) the T cell mitogen concanavalin A (Con A), and (c) the B cell mitogen lipopolysaccharide (LPS). The cultures were pulsed for 10 h with (14)C-labeled galactose and glucosamine. Radiolabeled glycosphingolipids...

journal_title：Biochemistry

authors： Gruner KR,van Eijk RV,Mühlradt PF

更新日期：1981-08-04 00:00:00

abstract：:Collagen is a multifunctional protein, serving as a structural scaffold and a modulator of cellular responses. Prior work has identified distinct regions from several collagen types that promote cell adhesion, spreading, migration, and signal transduction. One of these regions, alpha1(IV)1263-1277 from type IV collage...

journal_title：Biochemistry

authors： Malkar NB,Lauer-Fields JL,Borgia JA,Fields GB

更新日期：2002-05-14 00:00:00

abstract：:The dual specificity mitogen-activated protein kinase phosphatase MKP3 downregulates mitogenic signaling through dephosphorylation of extracellular signal-regulated kinase (ERK). Like other MKPs, MKP3 consists of a noncatalytic N-terminal domain and a catalytic C-terminal domain. ERK binding to the N-terminal noncatal...

journal_title：Biochemistry

authors： Kim Y,Rice AE,Denu JM

更新日期：2003-12-30 00:00:00

abstract：:In the presence of Ca2+ and calmodulin (CM), purified smooth muscle myosin light chain kinase (MLCKase) was found to undergo autophosphorylation at a rate that was about 200-fold slower than its catalytic activity. Up to 1.7 mol of phosphate were incorporated per mole of kinase. Lower levels of incorporation could be ...

journal_title：Biochemistry

authors： Sobieszek A

更新日期：1995-09-19 00:00:00

abstract：:A type II topoisomerase is essential for decatenating DNA replication products, and it accomplishes this task by passing one DNA duplex through a transient break in a second duplex. The B' domain of topoisomerase II contains three highly conserved motifs, EGDSA, PL(R/K)GK(I/L/M)LNVR, and IMTD(Q/A)DXD. We have investig...

journal_title：Biochemistry

authors： West KL,Meczes EL,Thorn R,Turnbull RM,Marshall R,Austin CA

更新日期：2000-02-15 00:00:00

abstract：:In this paper, we report kinetic studies for the chymotryptic activity of the 20S proteasome. Major observations include the following: (1) Reaction progress curves that are recorded at concentrations of Suc-Leu-Leu-Val-Tyr-AMC greater than about 40 microM are biphasic and characterized by initial velocities that deca...

journal_title：Biochemistry

authors： Stein RL,Melandri F,Dick L

更新日期：1996-04-02 00:00:00

abstract：:Escherichia coli MutS protein, which is required for mismatch repair, has a slow ATPase activity that obeys Michalelis-Menten kinetics. At 37 degrees C, the steady-state turnover rate for ATP hydrolysis is 1.0 +/- 0.3 min(-1) per monomer equivalent with a K(m) of 33 +/- 6 microM. Hydrolysis is competitively inhibited ...

journal_title：Biochemistry

authors： Bjornson KP,Allen DJ,Modrich P

更新日期：2000-03-21 00:00:00

abstract：:Anatoxin-a and homoanatoxin-a are two potent cyanobacterial neurotoxins. We recently reported the identification of the gene cluster responsible for the biosynthesis of these toxins in cyanobacteria and proposed a biosynthetic scheme starting from L-proline and involving three polyketide synthases for which the starte...

journal_title：Biochemistry

authors： Méjean A,Mann S,Vassiliadis G,Lombard B,Loew D,Ploux O

更新日期：2010-01-12 00:00:00

abstract：:Peptides and peptide-like molecules as a class have very poor permeability through biological membranes, which severely compromises their potential effectiveness as therapeutic agents. In order to gain insight into the problem of delivering peptide and protein drugs and to establish a model in which the effects of sys...

journal_title：Biochemistry

authors： Gray RA,Vander Velde DG,Burke CJ,Manning MC,Middaugh CR,Borchardt RT

更新日期：1994-02-15 00:00:00

abstract：:Due to their prevalence and roles in biological systems, single mismatches adjacent to G-U pairs are important RNA structural elements. Since there are only limited experimental values for the stability of single mismatches adjacent to G-U pairs, current algorithms using free energy minimization to predict RNA seconda...

journal_title：Biochemistry

authors： Davis AR,Znosko BM

更新日期：2008-09-23 00:00:00

abstract：:In the absence of any external electron donor, the "peroxy" intermediate of cytochrome c oxidase (CcO-607) is converted to the ferryl form (CcO-580) and subsequently to oxidized enzyme. The rate of conversion of CcO-607 to the CcO-580 form is pH dependent between pH 3.0 and pH 7.6. A plot of the logarithm of the rate ...

journal_title：Biochemistry

authors： Fabian M,Palmer G

更新日期：2001-02-13 00:00:00

abstract：:Epidermal growth factor receptor (EGFR) exemplifies the family of receptor tyrosine kinases that mediate numerous cellular processes, including growth, proliferation, and differentiation. Moreover, gene amplification and EGFR mutations have been identified in a number of human malignancies, making this receptor an imp...

journal_title：Biochemistry

authors： Truong TH,Carroll KS

更新日期：2012-12-18 00:00:00

abstract：:Hemoglobins extracted from fishes that live in temperate waters show little or no dissociation even in the liganded form, unlike human hemoglobin (HbA). To establish whether cold adaptation influences the tendency to dissociate, the dimer-tetramer association constants (L(2,4)) of the carbonmonoxy derivatives of repre...

journal_title：Biochemistry

authors： Giangiacomo L,D'Avino R,di Prisco G,Chiancone E

更新日期：2001-03-13 00:00:00

abstract：:Purified subunits of intermediate filaments obtained from a variety of tissues and cell types contain O-phosphoserine and, in some cases, smaller amounts of O-phosphothreonine. The O-phosphoserine content was estimated by reaction of performic acid oxidized subunits with methylamine in NaOH. Decamin of BHK-21 and CHO ...

journal_title：Biochemistry

authors： Steinert PM,Wantz ML,Idler WW

更新日期：1982-01-05 00:00:00

abstract：:We have used a new fluorescent ATP analogue, 3'-(7-diethylaminocoumarin-3-carbonylamino)-3'-deoxyadenosine-5'-triphosphate (deac-aminoATP), to study the ATP hydrolysis mechanism of the single headed myosinV-S1. Our study demonstrates that deac-aminoATP is an excellent substrate for these studies. Although the deac-ami...

journal_title：Biochemistry

authors： Forgacs E,Cartwright S,Kovács M,Sakamoto T,Sellers JR,Corrie JE,Webb MR,White HD

更新日期：2006-10-31 00:00:00