Role of 2',5'-oligo(adenylic acid) polymerase in the degradation of ribonucleic acid linked to double-stranded ribonucleic acid by extracts of interferon-treated cells.

abstract：:Many DNA polymerases select their natural substrates, deoxy- as opposed to ribonucleoside triphosphates, with a selectivity greater than 10000-fold. The function of a highly conserved residue, Tyr416, in the palm domain of the parental enzyme, an exo(-) derivative of RB69 DNA polymerase (gp43), a member of the pol alp...

journal_title：Biochemistry

authors： Yang G,Franklin M,Li J,Lin TC,Konigsberg W

更新日期：2002-08-13 00:00:00

abstract：:Fesselin is a proline-rich actin-binding protein that was isolated from avian smooth muscle. Fesselin bundles actin and accelerates actin polymerization by facilitating nucleation. We now show that this polymerization of actin can be regulated by Ca(2+)-calmodulin. Fesselin was shown to bind to immobilized calmodulin ...

journal_title：Biochemistry

authors： Schroeter M,Chalovich JM

更新日期：2004-11-02 00:00:00

abstract：:Using solution-state NMR spectroscopy, new insights into the early events governing amelogenin supramolecular self-assembly have been identified using sodium chloride and calcium chloride to trigger the association. Two-dimensional 1H-15N HSQC spectra were recorded for 15N- and 13C-labeled murine amelogenin as a funct...

journal_title：Biochemistry

authors： Buchko GW,Tarasevich BJ,Bekhazi J,Snead ML,Shaw WJ

更新日期：2008-12-16 00:00:00

abstract：:Three spin-labeled derivatives of the voltage-gated peptide alamethicin were prepared with nitroxides at the C-terminal phenyalaninol, and at positions 9 and 15 in the amino acid sequence. In addition, three spin-labeled derivatives of an analog of alamethicin where alpha-methylalanine residues are replaced by leucine...

journal_title：Biochemistry

authors： Barranger-Mathys M,Cafiso DS

更新日期：1996-01-16 00:00:00

abstract：:In eukaryotes, choline acetyltransferase (ChAT) catalyzes the reversible formation of the neurotransmitter acetylcholine from choline and acetyl-CoA. ChAT belongs to a family of CoA-dependent enzymes that also includes the carnitine acyltransferases CrAT, CrOT, and CPTs. In contrast to CrOT and CPTs that are very acti...

journal_title：Biochemistry

authors： Green KD,Porter VR,Zhang Y,Garneau-Tsodikova S

更新日期：2010-07-27 00:00:00

abstract：:Catalytic promiscuity is a widespread, but poorly understood, phenomenon among enzymes with particular relevance to the evolution of new functions, drug metabolism, and in vitro biocatalyst engineering. However, there is at present no way to quantitatively measure or compare this important parameter of enzyme function...

journal_title：Biochemistry

authors： Nath A,Atkins WM

更新日期：2008-01-08 00:00:00

abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of d-glyceraldehyde 3-phosphate (GAP) into d-glycerate 1,3-bisphosphate (1,3-diPG) in the presence of NAD(+) and inorganic phosphate (P(i)). Within the active site, two anion-binding sites were ascribed to the binding o...

journal_title：Biochemistry

authors： Castilho MS,Pavão F,Oliva G,Ladame S,Willson M,Périé J

更新日期：2003-06-17 00:00:00

abstract：:The high molecular weight folate binding protein of rat liver cytosol has been purified to apparent homogeneity. Purification was achieved by using a combination of gel filtration, O-(diethylaminoethyl)cellulose chromatography, and affinity chromatography. This folate binding protein was initially identified during pu...

journal_title：Biochemistry

authors： Cook RJ,Wagner C

更新日期：1982-08-31 00:00:00

abstract：:The effect of long-term storage on the electronic absorption spectrum and the kinetic properties of cytochrome c peroxidase has been investigated. No detectable differences were observed between freshly isolated enzyme and enzyme stored below -20 degrees C, in the crystalline state, for up to 41 months. The electronic...

journal_title：Biochemistry

authors： Vitello LB,Huang M,Erman JE

更新日期：1990-05-08 00:00:00

abstract：:The dependence of calcineurin on Ca2+ for activity is the result of the concerted action of calmodulin, which increases the turnover rate of the enzyme and modulates its response to Ca2+ transients, and of calcineurin B, which decreases the Km of the enzyme for its substrate. The calmodulin-stimulated protein phosphat...

journal_title：Biochemistry

authors： Stemmer PM,Klee CB

更新日期：1994-06-07 00:00:00

abstract：:Ammonia binds directly to the oxygen-evolving complex of photosystem II (PSII) upon formation of the S2 intermediate, as evidenced by electron paramagnetic resonance spectroscopy. We explore the binding mode by using quantum mechanics/molecular mechanics methods and simulations of extended X-ray absorption fine struct...

journal_title：Biochemistry

authors： Askerka M,Vinyard DJ,Brudvig GW,Batista VS

更新日期：2015-09-29 00:00:00

abstract：:Telomerase is a ribonucleoprotein that participates in the maintenance of telomere length. Its activity is up-regulated in many tumor types, suggesting that it may be a novel target for chemotherapy. The RNA component of telomerase contains an active site that plays at least two roles&sbd;binding telomere ends and tem...

journal_title：Biochemistry

authors： Hamilton SE,Pitts AE,Katipally RR,Jia X,Rutter JP,Davies BA,Shay JW,Wright WE,Corey DR

更新日期：1997-09-30 00:00:00

abstract：:The fluorescent nucleotide analogue of ADP, 1,N6-ethenoadenosine diphosphate (epsilon ADP), has been used to probe the active site of myosin subfragment 1 (SF1). The Mg complex of ADP was shown to be trapped stoichiometrically at the active site by a variety of thiol cross-linking agents having sulfur to sulfur spanni...

journal_title：Biochemistry

authors： Perkins WJ,Wells JA,Yount RG

更新日期：1984-08-14 00:00:00

abstract：:A cutinase-type polyesterase from Saccharomonospora viridis AHK190 (Cut190) has been shown to degrade the inner block of polyethylene terephthalate. A unique feature of Cut190 is that its function and stability are regulated by Ca2+ binding. Our previous crystal structure analysis of Cut190S226P showed that one Ca2+ b...

journal_title：Biochemistry

authors： Numoto N,Kamiya N,Bekker GJ,Yamagami Y,Inaba S,Ishii K,Uchiyama S,Kawai F,Ito N,Oda M

更新日期：2018-09-11 00:00:00

abstract：:The dynamics of the single-stranded, helix--coil transition of poly(adenylic acid) have been investigated by using the Raman laser temperature jump technique. The driving forces of this conformational transition have been probed by varying the cosolvent in mixed aqueous solutions. The rate of helix formation correlate...

journal_title：Biochemistry

authors： Dewey TG,Turner DH

更新日期：1980-04-15 00:00:00

abstract：:Previous work has indicated that following the rapid adenosine 5'-triphosphate (ATP) induced dissociation of the microtubule-dynein complex, the rate-limiting step in the ATPase cycle is product release [Johnson, K. A. (1983) J. Biol. Chem. 258, 13825-13832], which occurs at a rate of approximately 2-6 s-1. In this re...

journal_title：Biochemistry

authors： Omoto CK,Johnson KA

更新日期：1986-01-28 00:00:00

abstract：:The plasma membrane serotonin transporter (SERT) has an important role in terminating serotonergic neurotransmission by re-uptake of 5-HT from the synaptic cleft. The expression of SERT on the cell surface is therefore a critical factor. In this study, we examined the role of the carboxyl terminus of SERT in trafficki...

journal_title：Biochemistry

authors： Larsen MB,Fjorback AW,Wiborg O

更新日期：2006-01-31 00:00:00

abstract：:Sf9 cells infected with a recombinant baculovirus containing the gene for human prorenin were cultured in the presence of [3H]mannose. In vivo labeled prorenin was isolated by immunoprecipitation from the culture medium and digested with Pronase. The oligosaccharide structures on the resulting glycopeptides were analy...

journal_title：Biochemistry

authors： Aeed PA,Elhammer AP

更新日期：1994-07-26 00:00:00

abstract：:Human S-adenosylmethionine decarboxylase (AdoMetDC) was expressed in high yield in Escherichia coli using the pIN-III(lppP-5) expression vector and purified to apparent homogeneity using affinity chromatography on methylglyoxal bis(guanylhydrazone)-Sepharose. The inactivation of the purified enzyme by 5'-deoxy-5'-[(3-...

journal_title：Biochemistry

authors： Shantz LM,Stanley BA,Secrist JA 3rd,Pegg AE

更新日期：1992-07-28 00:00:00

abstract：:To identify interactions between Ste2p, a G protein-coupled receptor of the yeast Saccharomyces cerevisiae, and its tridecapeptide ligand, alpha-factor (WHWLQLKPGQPMY), a variety of alpha-factor analogues were used in conjunction with site-directed mutagenesis of a targeted portion of Ste2p transmembrane domain six. A...

journal_title：Biochemistry

authors： Lee BK,Lee YH,Hauser M,Son CD,Khare S,Naider F,Becker JM

更新日期：2002-11-19 00:00:00

abstract：:The metal-dependent deacetylase LpxC catalyzes the first committed step of lipid A biosynthesis in Gram-negative bacteria. Accordingly, LpxC is an attractive target for the development of inhibitors that may serve as potential new antibiotics for the treatment of Gram-negative bacterial infections. Here, we report the...

journal_title：Biochemistry

authors： Gennadios HA,Whittington DA,Li X,Fierke CA,Christianson DW

更新日期：2006-07-04 00:00:00

abstract：:Site-directed mutagenesis and high-resolution two-dimensional (2D) proton nuclear magnetic resonance (NMR) were used to probe the structural and functional roles of a highly conserved residue, Asp-49, in the interfacial catalysis by bovine pancreatic phospholipase A2 (PLA2, overexpressed in Escherichia coli). Accordin...

journal_title：Biochemistry

authors： Li Y,Yu BZ,Zhu H,Jain MK,Tsai MD

更新日期：1994-12-13 00:00:00

abstract：:Protein prenylation involves the attachment of a hydrophobic isoprenoid moiety to the C-terminus of proteins. Several small GTPases, including members of the Ras and Rho subfamilies, require prenylation for their normal and pathological functions. Recent work has suggested that SmgGDS proteins regulate the prenylation...

journal_title：Biochemistry

authors： Jennings BC,Lawton AJ,Rizk Z,Fierke CA

更新日期：2018-07-24 00:00:00

abstract：:Stability increments of terminal unpaired nucleotides (dangling ends) and terminal base pairs on the core helixes AUGCAU and UGCGCA are reported. Enthalpy, entropy, and free energy changes of helix formation were measured spectrophotometrically for 18 oligoribonucleotides containing the core sequences. The results ind...

journal_title：Biochemistry

authors： Sugimoto N,Kierzek R,Turner DH

更新日期：1987-07-14 00:00:00

abstract：:The C2A domain of synaptotagmin 7 (Syt7) is a Ca(2+) and membrane binding module that docks and inserts into cellular membranes in response to elevated intracellular Ca(2+) concentrations. Like other C2 domains, Syt7 C2A binds Ca(2+) and membranes primarily through three loop regions; however, it docks at Ca(2+) conce...

journal_title：Biochemistry

authors： Chon NL,Osterberg JR,Henderson J,Khan HM,Reuter N,Knight JD,Lin H

更新日期：2015-09-22 00:00:00

abstract：:A bifunctional molecule was genetically engineered which contained an amino-terminal effector domain that bound immunoglobulin Fc (fragment B of staphylococcal protein A) and a carboxyl-terminal domain that bound digoxin [a single-chain Fv (sFv)]. Effector and sFv binding properties were virtually identical with those...

journal_title：Biochemistry

authors： Tai MS,Mudgett-Hunter M,Levinson D,Wu GM,Haber E,Oppermann H,Huston JS

更新日期：1990-09-04 00:00:00

abstract：:The human ferritin L-chain cDNA was cloned into a vector for overproduction in Escherichia coli, under the regulation of a lambda promoter. The plasmid obtained contains the full L-chain coding region modified at the first two codons. It is able to direct the synthesis of the L-chain which can constitute up to 15% of ...

journal_title：Biochemistry

authors： Levi S,Salfeld J,Franceschinelli F,Cozzi A,Dorner MH,Arosio P

更新日期：1989-06-13 00:00:00

abstract：:The linkage between substrate and regulatory effector binding to separate sites on allosteric enzymes results in shifts in their sigmoidal kinetics to regulate metabolism. Control of branched chain amino acid biosynthesis in Escherichia coli occurs in part through shifts in the sigmoidal dependence of alpha-ketobutyra...

journal_title：Biochemistry

authors： Eisenstein E,Yu HD,Fisher KE,Iacuzio DA,Ducote KR,Schwarz FP

更新日期：1995-07-25 00:00:00

abstract：:Positively charged cyclic hexapeptide Phe-Arg-Cys-Arg-Cys-Phe-CONH2 (FRCRCFa) represents a group of conformationally constrained peptides that block the cardiac sarcolemma Na(+)-Ca2+ exchanger [Khananshvili et al. (1995b) J. Biol. Chem. 270, 16182-16188]. Here, we study the kinetic mechanisms of FRCRCFa-induced inhibi...

journal_title：Biochemistry

authors： Khananshvili D,Baazov D,Weil-Maslansky E,Shaulov G,Mester B

更新日期：1996-12-10 00:00:00

abstract：:The FAD-dependent alpha-glycerophosphate oxidase (GlpO) from Enterococcus casseliflavus and Streptococcus sp. was originally studied as a soluble flavoprotein oxidase; surprisingly, the GlpO sequence is 30-43% identical to those of the alpha-glycerophosphate dehydrogenases (GlpDs) from mitochondrial and bacterial sour...

journal_title：Biochemistry

authors： Colussi T,Parsonage D,Boles W,Matsuoka T,Mallett TC,Karplus PA,Claiborne A

更新日期：2008-01-22 00:00:00