Redesign of cosubstrate specificity and identification of important residues for substrate binding to hChAT.

abstract：:Superoxide reductase (SOR) is an Fe protein that catalyzes the reduction of superoxide to give H(2)O(2). Recently, the mutation of the Glu47 residue into alanine (E47A) in the active site of SOR from Desulfoarculus baarsii has allowed the stabilization of an iron-peroxo species when quickly reacted with H(2)O(2) [Math...

journal_title：Biochemistry

authors： Horner O,Mouesca JM,Oddou JL,Jeandey C,Nivière V,Mattioli TA,Mathé C,Fontecave M,Maldivi P,Bonville P,Halfen JA,Latour JM

更新日期：2004-07-13 00:00:00

abstract：:A homogeneous preparation of transketolase was obtained from spinach leaf; the specific enzyme activity was 9.5 mumolo of glyceraldehyde-3-P formed (mg of protein)-1 min-1, when xylulose-5-P and ribose-5-P were used as the donor and acceptor, respectively, of the ketol residue. Transketolase catalyzed the formation of...

journal_title：Biochemistry

authors： Takabe T,Asami S,Akazawa T

更新日期：1980-08-19 00:00:00

abstract：:A pterin analogue, 5-[(3-azido-6-nitrobenzylidene)amino]-2,6-diamino-4-pyrimidinone (ANBADP), was synthesized as a probe of the pterin binding site of phenylalanine hydroxylase. The photoaffinity label has been found to be a competitive inhibitor of the enzyme with respect to 6,7-dimethyltetrahydropterin, having a Ki ...

journal_title：Biochemistry

authors： Gibbs BS,Benkovic SJ

更新日期：1991-07-09 00:00:00

abstract：:The specificity of subtilisin BPN' toward substrates with large hydrophobic P4 residues has been improved by single amino acid replacements at positions 104 and 107. Mutations were designed to (i) increase the size of the P4 binding pocket by replacing Ile107, which is at the bottom of the S4 pocket, by Val, Ala, and ...

journal_title：Biochemistry

authors： Rheinnecker M,Baker G,Eder J,Fersht AR

更新日期：1993-02-09 00:00:00

abstract：:The S-protein/vitronectin gene was isolated from a human genomic DNA library, and its sequence of about 5.3 kilobases including the adjacent 5' and 3' flanking regions was established. Alignment of the genomic DNA nucleotide sequence and the cDNA sequence indicated that the gene consisted of eight exons and seven intr...

journal_title：Biochemistry

authors： Jenne D,Stanley KK

更新日期：1987-10-20 00:00:00

abstract：:The active-site zinc in human glutathione-dependent formaldehyde dehydrogenase (FDH) undergoes coenzyme-induced displacement and transient coordination to a highly conserved glutamate residue (Glu-67) during the catalytic cycle. The role of this transient coordination of the active-site zinc to Glu-67 in the FDH catal...

journal_title：Biochemistry

authors： Sanghani PC,Davis WI,Zhai L,Robinson H

更新日期：2006-04-18 00:00:00

abstract：:The NADH absorbance spectrum of nicotinoprotein (NADH-containing) alcohol dehydrogenase from Amycolatopsis methanolica has a maximum at 326 nm. Reduced enzyme-bound pyridine dinucleotide could be reversibly oxidized by acetaldehyde. The fluorescence excitation spectrum for NADH bound to the enzyme has a maximum at 325...

journal_title：Biochemistry

authors： Piersma SR,Visser AJ,de Vries S,Duine JA

更新日期：1998-03-03 00:00:00

abstract：:In this paper the interaction of cytoplasmic CopZ and the N-terminal domain of the CopA ATPase from Bacillus subtilis has been studied by NMR through (15)N-(1)H HSQC experiments in order to understand the role of the two proteins in the whole copper trafficking mechanism of the bacteria. It appears that the two protei...

journal_title：Biochemistry

authors： Banci L,Bertini I,Ciofi-Baffoni S,Del Conte R,Gonnelli L

更新日期：2003-02-25 00:00:00

abstract：:Streptomyces griseus trypsin has been isolated from Pronase by ion-exchange chromatography on CM-Sephadex and SE-Sephadex. The isolated enzyme was homogeneous by the criteria tested except for a low degree of contamination by an enzyme with nontryptic activity. The latter could be partially resolved by chromatography ...

journal_title：Biochemistry

authors： Olafson RW,Smillie LB

更新日期：1975-03-25 00:00:00

abstract：:The spin equilibria of several derivatives of human methemoglobin were probed by resonance Raman scattering. The intensity of lines in the Raman spectrum gives a measure of the high-spin (S = 5/2) to low-spin (S = 1/2) ratio which agrees well with the spin equilibria determined from direct magnetic susceptibility meas...

journal_title：Biochemistry

authors： Noble RW,DeYoung A,Rousseau DL

更新日期：1989-06-13 00:00:00

abstract：:Common structural motifs, such as the cupin domains, are found in enzymes performing different biochemical functions while retaining a similar active site configuration and structural scaffold. The soil bacterium Bacillus subtilis has 20 cupin genes (0.5% of the total genome) with up to 14% of its genes in the form of...

journal_title：Biochemistry

authors： Gopal B,Madan LL,Betz SF,Kossiakoff AA

更新日期：2005-01-11 00:00:00

abstract：:Studies were undertaken to assess the postulated involvement of subunit III in the proton-linked functions of cytochrome c oxidase. The effect of pH on the steady-state kinetic [corrected] parameters of subunit III containing and subunit III depleted cytochrome oxidase was determined by using beef heart and rat liver ...

journal_title：Biochemistry

authors： Gregory LC,Ferguson-Miller S

更新日期：1988-08-23 00:00:00

abstract：:Thrombospondin-1 (TSP1) binding to calreticulin (CRT) on the cell surface signals focal adhesion disassembly, leading to the intermediate adhesive phenotype, cell migration, anoikis resistance, and collagen stimulation. Residues Lys 24 and 32 in TSP1 and amino acids 24-26 and 32-34 in CRT have been shown through bioch...

journal_title：Biochemistry

authors： Yan Q,Murphy-Ullrich JE,Song Y

更新日期：2011-02-01 00:00:00

abstract：:Bacillus thuringiensis (Bt) insecticidal toxins bind to receptors on midgut epithelial cells of susceptible insects, and binding triggers biochemical events that lead to insect mortality. Recently, a 100-kDa aminopeptidase N (APN) was isolated from brush border membrane vesicles (BBMV) of Anopheles quadrimaculatus and...

journal_title：Biochemistry

authors： Zhang R,Hua G,Andacht TM,Adang MJ

更新日期：2008-10-28 00:00:00

abstract：:Affinity selection-mass spectrometry (AS-MS) screening of kinesin spindle protein (KSP) followed by enzyme inhibition studies and temperature-dependent circular dichroism (TdCD) characterization was utilized to identify a series of benzimidazole compounds. This series also binds in the presence of Ispinesib, a known a...

journal_title：Biochemistry

authors： Sheth PR,Shipps GW Jr,Seghezzi W,Smith CK,Chuang CC,Sanden D,Basso AD,Vilenchik L,Gray K,Annis DA,Nickbarg E,Ma Y,Lahue B,Herbst R,Le HV

更新日期：2010-09-28 00:00:00

abstract：:Transport of protons and solutes across mitochondrial membranes is essential for many physiological processes. However, neither the proton-pumping respiratory chain complexes nor the mitochondrial secondary active solute transport proteins have been characterized electrophysiologically in their native environment. In ...

journal_title：Biochemistry

authors： Watzke N,Diekert K,Obrdlik P

更新日期：2010-12-07 00:00:00

abstract：:Binding of the nonhydrolyzable ATP analogue 5'-adenylyl-beta, gamma-imidodiphosphate (ADPNP) to Escherichia coli DNA gyrase can lead to a limited noncatalytic supercoiling of DNA. Here we examine the efficiency of coupling between ADPNP binding and the change in linking number either of positively or negatively superc...

journal_title：Biochemistry

authors： Bates AD,O'Dea MH,Gellert M

更新日期：1996-02-06 00:00:00

abstract：:Heat shock protein 90 (Hsp90) is a highly conserved ATP-driven machine involved in client protein maturation, folding, and activation. The chaperone is supported by a set of cochaperones that confer client specificities. One of those proteins is the suppressor of G2 allele of skp1 (Sgt1), which participates together w...

journal_title：Biochemistry

authors： Eckl JM,Drazic A,Rutz DA,Richter K

更新日期：2014-04-22 00:00:00

abstract：:The isothermal equilibrium folding of the unmodified yeast tRNA(Phe) is studied as a function of Na(+), Mg(2+), and urea concentration with hydroxyl radical protection, circular dichroism, and diethyl pyrocarbonate (DEPC) modification. These assays indicate that this tRNA folds in Na(+) alone. Similar to folding in Mg...

journal_title：Biochemistry

authors： Shelton VM,Sosnick TR,Pan T

更新日期：2001-03-27 00:00:00

abstract：:In order to test the proposal [Stehle, T., Claiborne, A., & Schulz, G. E. (1993) Eur. J. Biochem. 211, 221-226] that the active-site His10 of NADH peroxidase functions as an essential acid-base catalyst, we have analyzed mutants in which this residue has been replaced by Gln or Ala. The k(cat) values for both H10Q and...

journal_title：Biochemistry

authors： Crane EJ 3rd,Parsonage D,Claiborne A

更新日期：1996-02-20 00:00:00

abstract：:Decapping is the first committed step in 5'-to-3' RNA decay, and in the cytoplasm of human cells, multiple decapping enzymes regulate the stabilities of distinct subsets of cellular transcripts. However, the complete set of RNAs regulated by any individual decapping enzyme remains incompletely mapped, and no consensus...

journal_title：Biochemistry

authors： Luo Y,Schofield JA,Simon MD,Slavoff SA

更新日期：2020-11-03 00:00:00

abstract：:1H NMR and ligand-binding data were used to assess the role of residue Arg(E10) in ligand stabilization of several site-directed mutants, all carrying the His(E7) to Val substitution, obtained using a synthetic sperm whale myoglobin gene. Arg(E10) was previously found to form a hydrogen bond with the ligand in fluoro-...

journal_title：Biochemistry

authors： Allocatelli CT,Cutruzzolà F,Brancaccio A,Brunori M,Qin J,La Mar GN

更新日期：1993-06-15 00:00:00

abstract：:We have used a site-specific approach to investigate the mutagenic potential of (+)- and (-)-trans-anti-benzo[a]pyrene diol epoxide (BPDE) DNA adducts. Oligodeoxyribonucleotides (5'TCCTCCTG1G2-CCTCTC), modified at the exocyclic amino groups of G1 or G2, were incorporated into a single-stranded shuttle vector and intro...

journal_title：Biochemistry

authors： Moriya M,Spiegel S,Fernandes A,Amin S,Liu T,Geacintov N,Grollman AP

更新日期：1996-12-24 00:00:00

abstract：:The sucrose permease (CscB) and lactose permease (LacY) of Escherichia coli belong to the oligosaccharide/H(+) symporter subfamily of the major facilitator superfamily, and both catalyze sugar/H(+) symport across the cytoplasmic membrane. Thus far, there is no common substrate for the two permeases; CscB transports su...

journal_title：Biochemistry

authors： Sugihara J,Smirnova I,Kasho V,Kaback HR

更新日期：2011-12-27 00:00:00

abstract：:Biotin synthase (BioB) is an iron-sulfur dimeric enzyme which catalyzes the last step in biotin synthesis. The reaction consists of the introduction of a sulfur atom into dethiobiotin. It is shown here that BioB displays a significant cysteine desulfurase activity, providing it with the ability to mobilize sulfur from...

journal_title：Biochemistry

authors： Ollagnier-De-Choudens S,Mulliez E,Hewitson KS,Fontecave M

更新日期：2002-07-23 00:00:00

abstract：:The oxidation pathway of chlorophyll Z (ChlZ) in photosystem II (PSII) at cryogenic temperatures was studied by means of light-induced Fourier transform infrared (FTIR) difference spectroscopy. To examine the involvement of redox-active beta-carotene (Car) in the pathway, two Car molecules in Mn-depleted PSII membrane...

journal_title：Biochemistry

authors： Kitajima Y,Noguchi T

更新日期：2006-02-14 00:00:00

abstract：:Extracellular matrix (ECM) proteins, such as collagen and fibronectin, play vital roles in development and maintenance of hard tissue (bone or tooth) and are, consequently, thoroughly investigated for construction of biomimetic scaffolds in combination with calcium phosphate (CaP) material (the major component of hard...

journal_title：Biochemistry

authors： Chowdhury EH,Nagaoka M,Ogiwara K,Zohra FT,Kutsuzawa K,Tada S,Kitamura C,Akaike T

更新日期：2005-09-20 00:00:00

abstract：:A model for radiation-induced cross-linking of DNA and proteins has been developed. It is based on initial formation of free radicals on a DNA base, i.e., thymine, and on an amino acid, i.e., tyrosine. It was demonstrated that interaction of these radicals is highly favored as measured by their kinetics and the cross-...

journal_title：Biochemistry

authors： Simic MG,Dizdaroglu M

更新日期：1985-01-01 00:00:00

abstract：:The effect of tropomyosin alone on the binding of myosin subfragment 1 (S-1) to actin was compared to that of troponin-tropomyosin, with and without Ca2+. Over a range of ionic strength and with different ligands (adenyl-5'-yl imidodiphosphate, pyrophosphate, and adenosine 5'-diphosphate), tropomyosin confers slight c...

journal_title：Biochemistry

authors： Williams DL Jr,Greene LE

更新日期：1983-05-24 00:00:00

abstract：:The biosynthesis of ansamycin antibiotics, including rifamycin B, involves the synthesis of an aromatic precursor, 3-amino-5-hydroxybenzoic acid (AHBA), which serves as starter for the assembly of the antibiotics' polyketide backbone. The terminal enzyme of AHBA formation, AHBA synthase, is a dimeric, pyridoxal 5'-pho...

journal_title：Biochemistry

authors： Eads JC,Beeby M,Scapin G,Yu TW,Floss HG

更新日期：1999-08-03 00:00:00