Redesign of cosubstrate specificity and identification of important residues for substrate binding to hChAT.

abstract：:Thyroperoxidase (TPO), a type I transmembrane heme containing glycoprotein, catalyzes iodide organification and thyroid hormone synthesis. One of the two main alternatively spliced forms of this enzyme, TPOzanelli, which is present in Graves's disease thyroid tissue, has a cytoplasmic domain completely modified. In th...

journal_title：Biochemistry

authors： Niccoli-Sire P,Fayadat L,Siffroi-Fernandez S,Malthierry Y,Franc JL

更新日期：2001-02-27 00:00:00

abstract：:L-Aspartate oxidase (Laspo) catalyzes the conversion of L-Asp to iminoaspartate, the first step in the de novo biosynthesis of NAD(+). This bacterial pathway represents a potential drug target since it is absent in mammals. The Laspo R386L mutant was crystallized in the FAD-bound catalytically competent form and its t...

journal_title：Biochemistry

authors： Bossi RT,Negri A,Tedeschi G,Mattevi A

更新日期：2002-03-05 00:00:00

abstract：:Zinc finger nucleases (ZFNs) have been used to direct precise modifications of the genetic information in living cells at high efficiency. An important consideration in the design of ZFNs is the number of zinc fingers that are required for efficient and specific cleavage. We examined dimeric ZFNs composed of [1]+[1], ...

journal_title：Biochemistry

authors： Shimizu Y,Şöllü C,Meckler JF,Adriaenssens A,Zykovich A,Cathomen T,Segal DJ

更新日期：2011-06-07 00:00:00

abstract：:Significant concentrations (1-10 mM) of nickel(II) were found in solution after incubation of the potent carcinogen nickel subsulfide in 0.05 M Tris-HCl, pH 7.4, solutions containing DNA, rat liver microsomes, and NADPH. The presence of NADPH decreased the rate of solubilization of nickel subsulfide. The solubilized n...

journal_title：Biochemistry

authors： Lee JE,Ciccarelli RB,Jennette KW

更新日期：1982-02-16 00:00:00

abstract：:UreE is a homodimeric metallo-chaperone that assists the insertion of Ni(2+) ions in the active site of urease. The crystal structures of UreE from Bacillus pasteurii and Klebsiella aerogenes have been determined, but the details of the nickel-binding site were not elucidated due to solid-state effects that caused dis...

journal_title：Biochemistry

authors： Stola M,Musiani F,Mangani S,Turano P,Safarov N,Zambelli B,Ciurli S

更新日期：2006-05-23 00:00:00

abstract：:The RING or Really Interesting New Gene represents a family of eukaryotic sequences that bind Zn (II) ions and participate in intracellular processes involving protein-protein interaction. Although found in over 400 different proteins, very little is known regarding the structure-function properties of these domains b...

journal_title：Biochemistry

authors： Collino S,Kim IW,Evans JS

更新日期：2008-03-25 00:00:00

abstract：:Initiation of RNA synthesis by Bacillus subtilis RNA polymerase (sigma-43) has been examined at two early promoters of phage phi 29: the A2 promoter, which is a weak promoter, and the G2 promoter, which is a strong promoter. The delta subunit of the polymerase inhibits the rate of initiation at A2, but not G2. In addi...

journal_title：Biochemistry

authors： Dobinson KF,Spiegelman GB

更新日期：1987-12-15 00:00:00

abstract：:The binding modes of four active site-directed, acylating inhibitors of human alpha-thrombin have been determined using X-ray crystallography. These inhibitors (GR157368, GR166081, GR167088, and GR179849) are representatives of a series utilizing a novel 5, 5-trans-lactone template to specifically acylate Ser195 of th...

journal_title：Biochemistry

authors： Jhoti H,Cleasby A,Reid S,Thomas PJ,Weir M,Wonacott A

更新日期：1999-06-22 00:00:00

abstract：:Colibactins are genotoxic secondary metabolites whose biosynthesis is encoded in the clb gene cluster harbored by certain strains of gut commensal Escherichia coli. Using synthetic colibactin analogues, we previously provided evidence that colibactins alkylate DNA by addition of a nucleotide to an electrophilic cyclop...

journal_title：Biochemistry

authors： Xue M,Shine E,Wang W,Crawford JM,Herzon SB

更新日期：2018-11-13 00:00:00

abstract：:The interaction of a divalent metal ion with a leaving 3' oxygen is a central component of several proposed mechanisms of phosphoryl transfer. In support of this are recent kinetic studies showing that thiophilic metal ions (e.g., Mn2+) stimulate the hydrolysis of compounds in which sulfur takes the place of the leavi...

journal_title：Biochemistry

authors： Brautigam CA,Sun S,Piccirilli JA,Steitz TA

更新日期：1999-01-12 00:00:00

abstract：:Molecular dynamics (MD) simulations of Thermus thermophilus chorismate mutase substrate complex (TtCM x S) have been carried out at 298 K, 333 K, and the temperature of optimum activity: 343 K. The enzyme exists as trimeric subunits with active sites shared between two neighboring subunits. Two features distinguish in...

journal_title：Biochemistry

authors： Zhang X,Bruice TC

更新日期：2006-07-18 00:00:00

abstract：:It has been shown by genetic analysis that the external and internal invertases from Saccharomyces cerevisiae share a common structural gene [Taussig, R., & Carlson, M. (1983) Nucleic Acids Res. 11, 1943-1954]. However, the only amino acid composition of these two forms of invertase reported to date has revealed exten...

journal_title：Biochemistry

authors： Chu FK,Takase K,Guarino D,Maley F

更新日期：1985-10-22 00:00:00

abstract：:Aquaporin-4 (AQP4) is a water channel found at high concentrations around blood vessels in the brain and is organized into elaborate assemblies called square arrays. The natural functions of AQP4 and the square arrays remain unknown, but under pathophysiological conditions, AQP4 has been shown to influence brain edema...

journal_title：Biochemistry

authors： Strand L,Moe SE,Solbu TT,Vaadal M,Holen T

更新日期：2009-06-30 00:00:00

abstract：:Initial rate kinetic studies and reduced coenzyme binding studies with bovine glutamate dehydrogenase have shown that an enzyme-NAD(P)H-glutamate abortive complex is a major participant in the overall enzyme-catalyzed reaction. The allosteric regulator ADP is shown to activate the enzyme by destabilizing this abortive...

journal_title：Biochemistry

authors： George A,Bell JE

更新日期：1980-12-23 00:00:00

abstract：:Limited digestion of polysomal RNA with pancreatic ribonuclease releases a structure consisting of poly(A) associated with other polyribonucleotide sequences. This complex can be purified by oligo(dT)-cellulose chromatography. Heating for formamide treatment causes the dissociation of fragments free of poly(A) from th...

journal_title：Biochemistry

authors： Jeffery WR,Brawerman G

更新日期：1975-07-29 00:00:00

abstract：:Infrared (vibrational) circular dichroism (VCD) has been observed for the DNA models d(CG)5, poly(dG-dC).poly(dG-dC), poly(dG).poly(dC), poly(dA-dT).poly(dA-dT), and poly(dA).poly(dT) in the B-conformation in buffered, aqueous solution. The observed results are quantitatively interpreted in terms of the exciton model ...

journal_title：Biochemistry

authors： Zhong WX,Gulotta M,Goss DJ,Diem M

更新日期：1990-08-14 00:00:00

abstract：:The human translin protein binds a variety of sequences (chromosomal breakpoint consensus sequences, their sequence variants, as well as nonbreakpoint sequences such as simple AT and GC repeats) at nanomolar protein concentration when short single strands ( approximately 20-30mers) are used as DNA targets. The protein...

journal_title：Biochemistry

authors： Sengupta K,Rao BJ

更新日期：2002-12-24 00:00:00

abstract：:The folding kinetics of a 16-residue beta-hairpin (trpzip4) and five mutants were studied by a laser-induced temperature-jump infrared method. Our results indicate that mutations which affect the strength of the hydrophobic cluster lead to a decrease in the thermal stability of the beta-hairpin, as a result of increas...

journal_title：Biochemistry

authors： Du D,Tucker MJ,Gai F

更新日期：2006-02-28 00:00:00

abstract：:Galactose oxidase is a remarkable enzyme containing a metalloradical redox cofactor capable of oxidizing a variety of primary alcohols during enzyme turnover. Recent studies using 1-O-methyl alpha-D-galactopyranoside have revealed an unusually large kinetic isotope effect (KIE) for oxidation of the alpha-deuterated al...

journal_title：Biochemistry

authors： Whittaker MM,Whittaker JW

更新日期：2001-06-19 00:00:00

abstract：:In a previous report, the cDNA for human proteinase inhibitor 8 (PI8) was first identified, isolated, and subcloned into a mammalian expression vector and expressed in baby hamster kidney cells. Initial studies indicated that PI8 was able to inhibit the amidolytic activity of trypsin and form an SDS-stable approximate...

journal_title：Biochemistry

authors： Dahlen JR,Foster DC,Kisiel W

更新日期：1997-12-02 00:00:00

abstract：:Protein prenylation involves the attachment of a hydrophobic isoprenoid moiety to the C-terminus of proteins. Several small GTPases, including members of the Ras and Rho subfamilies, require prenylation for their normal and pathological functions. Recent work has suggested that SmgGDS proteins regulate the prenylation...

journal_title：Biochemistry

authors： Jennings BC,Lawton AJ,Rizk Z,Fierke CA

更新日期：2018-07-24 00:00:00

abstract：:Galectin-3 is a member of a large family of beta-galactoside-binding animal lectins and is composed of a carboxyl-terminal lectin domain connected to an amino-terminal nonlectin part. Previous experimental results suggest that, when bound to multivalent glycoconjugates, galectin-3 self-associates through intermolecula...

journal_title：Biochemistry

authors： Yang RY,Hill PN,Hsu DK,Liu FT

更新日期：1998-03-24 00:00:00

abstract：:Human acidic mammalian chitinase (AMCase), a member of the family 18 glycosyl hydrolases, is one of the important proteins involved in Th2-mediated inflammation and has been implicated in asthma and allergic diseases. Inhibition of AMCase results in decreased airway inflammation and airway hyper-responsiveness in a mo...

journal_title：Biochemistry

authors： Chou YT,Yao S,Czerwinski R,Fleming M,Krykbaev R,Xuan D,Zhou H,Brooks J,Fitz L,Strand J,Presman E,Lin L,Aulabaugh A,Huang X

更新日期：2006-04-11 00:00:00

abstract：:We have synthesized Deltalac-acetogenins that are new acetogenin mimics possessing two n-alkyl tails without an alpha,beta-unsaturated gamma-lactone ring and suggested that their inhibition mechanism may be different from that of common acetogenins [Hamada et al. (2004) Biochemistry 43, 3651-3658]. To elucidate the in...

journal_title：Biochemistry

authors： Ichimaru N,Murai M,Abe M,Hamada T,Yamada Y,Makino S,Nishioka T,Makabe H,Makino A,Kobayashi T,Miyoshi H

更新日期：2005-01-18 00:00:00

abstract：:It has been shown (Poole et al., 1991) that deletion of residues 44-49 from the sequence of staphylococcal nuclease (E43 SNase) results in an enzyme (E43 delta SNase) that is significantly more active than D43 SNase, an enzyme that differs from the wild-type enzyme by deletion of a single methylene group. In addition,...

journal_title：Biochemistry

authors： Baldisseri DM,Torchia DA,Poole LB,Gerlt JA

更新日期：1991-04-16 00:00:00

abstract：:The mechanisms of MRP1-drug binding and transport are not clear. In this study, we have characterized the interaction between MRP1 and rhodamine 123 (Rh123) using the photoreactive-iodinated analogue, [(125)I]iodoaryl azido-rhodamine 123 (or IAARh123). Photoaffinity labeling of plasma membranes from HeLa cells transfe...

journal_title：Biochemistry

authors： Daoud R,Kast C,Gros P,Georges E

更新日期：2000-12-19 00:00:00

abstract：:Nuclear Overhauser effect (NOE) measurements are reported for the nonexchangeable base and ribose 1' proton resonances of CCGGAp, ACCGGp, and ACCGGUp. The results permit assignment of these resonances to particular nucleotides in the sequences. The NOE data rule out conformations containing syn glycosidic linkages and...

journal_title：Biochemistry

authors： Petersheim M,Turner DH

更新日期：1983-01-18 00:00:00

abstract：:Pseudokinases play important roles in signal transduction and cellular processes similar to those of catalytically competent kinases. However, pseudokinase pharmacological tractability and conformational space accessibility are poorly understood. Pseudokinases have only recently been suggested to adopt "inactive" conf...

journal_title：Biochemistry

authors： Smith RHB,Khan ZM,Ung PM,Scopton AP,Silber L,Mack SM,Real AM,Schlessinger A,Dar AC

更新日期：2021-01-13 00:00:00

abstract：:In an effort to characterize the bimolecular interface between parathyroid hormone (PTH) and its human receptor PTH1-Rc (hPTH1-Rc), we previously identified two contact sites in the receptor: one for position 1 and another for position 13 (located at the ends of the principal activation domain) in PTH(1-34). The prese...

journal_title：Biochemistry

authors： Greenberg Z,Bisello A,Mierke DF,Rosenblatt M,Chorev M

更新日期：2000-07-18 00:00:00

abstract：:The mechanism of chromophore biosynthesis in green fluorescent protein (GFP) is triggered by a spontaneous main chain cyclization reaction of residues 65-67. Here, we demonstrate that the initially colorless Y66L variant, designed to trap chromophore precursor states, is oxidatively modified to generate yellow chromop...

journal_title：Biochemistry

authors： Rosenow MA,Patel HN,Wachter RM

更新日期：2005-06-14 00:00:00