Nuclear overhauser studies of CCGGAp, ACCGGp, and ACCGGUp.

abstract：:This work describes the biochemical characterization of the catalytic domain of Ira2p, a Saccharomyces cerevisiae GTPase-activating protein (GAP) regulating the RAS gene products. A fragment of 383 residues (amino acids 1644-2026) was produced in Escherichia coli as glutathione S-transferase fusion protein (GST-Ira2p-...

journal_title：Biochemistry

authors： Parrini MC,Jacquet E,Bernardi A,Jacquet M,Parmeggiani A

更新日期：1995-10-24 00:00:00

abstract：:The interaction of cholesterol with phospholipids has been studied with a variety of techniques; however, the possible consequences of such interactions in vivo have not been demonstrated. In this study, the cholesterol-dependent absorbance spectrum of cytochrome P-450scc was used to monitor cholesterol availability i...

journal_title：Biochemistry

authors： Stevens VL,Lambeth JD,Merrill AH Jr

更新日期：1986-07-29 00:00:00

abstract：:R67 dihydrofolate reductase (DHFR) is an R-plasmid encoded enzyme that confers resistance to the antibacterial drug trimethoprim. This enzyme is not homologous in sequence or structure to chromosomal DHFRs. Equilibrium folding of tetrameric R67 DHFR was studied and found to be fully reversible. Formation of an inactiv...

journal_title：Biochemistry

authors： Zhuang P,Eisenstein E,Howell EE

更新日期：1994-04-12 00:00:00

abstract：:Melittin is a 26-residue peptide which undergoes high-affinity calcium-dependent binding by calmodulin [Barnette, M.S., Daly, R., & Weiss, B. (1983) Biochem. Pharmacol. 32, 2929; Comte, M., Maulet, Y., & Cox, J.A. (1983) Biochem. J. 209, 269; Anderson, S.R., & Malencik, D.A. (1986) Calcium Cell Funct. 6, 1]. The resul...

journal_title：Biochemistry

authors： Malencik DA,Anderson SR

更新日期：1988-03-22 00:00:00

abstract：:Histidine-containing protein, HPr, is a phosphocarrier protein that is part of the bacterial phosphoenolpyruvate:sugar phosphotransferase system. HPr is phosphorylated by enzyme I, and P-HPr transfers the phosphoryl group to the IIA domain of a number of sugar-specific enzyme II complexes. Autocatalytic phosphoryl tra...

journal_title：Biochemistry

authors： Anderson JW,Waygood EB

更新日期：1993-06-08 00:00:00

abstract：:The folding of the enzyme indole-3-glycerol-phosphate synthase (IGPS), a member of the (alpha/beta)8 fold family, has been studied. At least two folding intermediates have been detected using spectroscopic and activity measurements in combination with gel filtration chromatography. These two intermediates are produced...

journal_title：Biochemistry

authors： Sánchez del Pino MM,Fersht AR

更新日期：1997-05-06 00:00:00

abstract：:An imidazole spin-label was used to study the role of tyrosyl residues in the reassociation process for the nucleosome core particle. The nucleosome core particle, containing 145 base pairs of DNA and a histone core (two each of the four histones H2S, H2B, H3, and H4), was isolated from chicken erythrocytes. Native pa...

journal_title：Biochemistry

authors： Chan DC,Piette LH

更新日期：1982-06-08 00:00:00

abstract：:Because of the lack of protein turnover in fiber cells of the ocular lens, Aquaporin 0 (AQP0), the most abundant membrane protein in the lens, undergoes extensive post-translational modification with fiber cell age. To map the distribution of modified forms of AQP0 within the lens, normal human lenses ranging in age f...

journal_title：Biochemistry

authors： Ball LE,Garland DL,Crouch RK,Schey KL

更新日期：2004-08-03 00:00:00

abstract：:Potent inhibitors of human cysteine proteases of the papain family have been made and assayed versus a number of relevant family members. We describe the synthesis of peptide alpha-ketoheterocyclic inhibitors that occupy binding subsites S1'-S3 of the cysteine protease substrate recognition cleft and that form a rever...

journal_title：Biochemistry

authors： McGrath ME,Sprengeler PA,Hill CM,Martichonok V,Cheung H,Somoza JR,Palmer JT,Janc JW

更新日期：2003-12-30 00:00:00

abstract：:The size and surface curvature dependence of the properties and stability of single lamellar vesicles have been investigated by using a variety of physicochemical techniques. Dipalmitoylphosphatidylcholine single lamellar vesicles of sizes ranging between 200 and 900 A in diameter have been prepared by the French pres...

journal_title：Biochemistry

authors： Lichtenberg D,Freire E,Schmidt CF,Barenholz Y,Felgner PL,Thompson TE

更新日期：1981-06-09 00:00:00

abstract：:Gelatinase B is a matrix metalloproteinase (MMP-9) involved in tissue remodeling, development, cancer, and inflammation. Neutrophils produce three major forms of (pro)gelatinase B: 92 kDa monomers, homodimers, and complexes of gelatinase B covalently bound to neutrophil gelatinase B-associated lipocalin (NGAL). In con...

journal_title：Biochemistry

authors： Rudd PM,Mattu TS,Masure S,Bratt T,Van den Steen PE,Wormald MR,Küster B,Harvey DJ,Borregaard N,Van Damme J,Dwek RA,Opdenakker G

更新日期：1999-10-19 00:00:00

abstract：:The question of the possible identity of catalytic and regulatory proton pathways in the chloroplast FoF1 ATPase has been studied using different energy-transfer inhibitors. Venturicidin, a reversible inhibitor of Fo, affects neither the delta mu H(+)-dependent thiol reduction of the membrane-bound chloroplast ATPase ...

journal_title：Biochemistry

authors： Valerio M,de Kouchkovsky Y,Haraux F

更新日期：1992-05-05 00:00:00

abstract：:Pepstatin is a slow and tight-binding inhibitor of pepsin. Preincubating enzyme and inhibitor in H2O and in D2O in the absence of substrate generates an inverse solvent isotope effect of Dk = 0.69 +/- 0.06 on the apparent first-order rate constant for the decay in enzymatic activity. Proton inventory analysis of the i...

journal_title：Biochemistry

authors： Cho YK,Rebholz KL,Northrop DB

更新日期：1994-08-16 00:00:00

abstract：:Three activities on the pathway of purine biosynthesis de novo in chicken liver, namely, glycinamide ribonucleotide synthetase, glycinamide ribonucleotide transformylase, and aminoimidazole ribonucleotide synthetase, have been found to reside on the same polypeptide chain. Three diverse purification schemes, utilizing...

journal_title：Biochemistry

authors： Daubner SC,Schrimsher JL,Schendel FJ,Young M,Henikoff S,Patterson D,Stubbe J,Benkovic SJ

更新日期：1985-12-03 00:00:00

abstract：:Ionizing radiation induces clustered DNA damage sites which have been shown to challenge the repair mechanism(s) of the cell. Evidence demonstrating that base excision repair is compromised during the repair of an abasic (AP) site present within a clustered damage site is presented. Simple bistranded clustered damage ...

journal_title：Biochemistry

authors： Lomax ME,Cunniffe S,O'Neill P

更新日期：2004-08-31 00:00:00

abstract：:1-Aminocyclopropanephosphonate (ACPP) was synthesized, and its effects on the pyridoxal 5'-phosphate linked enzymes 1-aminocyclopropanecarboxylate (ACPC) deaminase from Pseudomonas sp. ACPC and alanine racemase from Bacillus stearothermophilus were studied. ACPP was found to be a potent inhibitor of both enzymes with ...

journal_title：Biochemistry

authors： Erion MD,Walsh CT

更新日期：1987-06-16 00:00:00

abstract：:The N-terminal formamido group on imidazole- and pyrrole-containing polyamides causes stacked polyamides to bind in the minor groove of DNA in the staggered motif, and it also increases the binding affinity compared to those of non-formamido compounds. To further investigate the role of the N-terminal acylamido in aff...

journal_title：Biochemistry

authors： Westrate L,Mackay H,Brown T,Nguyen B,Kluza J,Wilson WD,Lee M,Hartley JA

更新日期：2009-06-23 00:00:00

abstract：:Factor VIII (fVIII) is synthesized as a single chain having a domainal sequence A1-A2-B-A3-C1-C2. Analysis of the proteolyic cleavage of fVIII by thrombin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) identifies three fragments designated fVIIIA1, fVIIIA2, and fVIIIA3-C1-C2 with fragment(s) d...

journal_title：Biochemistry

authors： Lollar P,Parker CG

更新日期：1989-01-24 00:00:00

abstract：:Yeast actin mutants with acidic residues at the N terminus either neutralized (DNEQ) or deleted (delta-DSE) were used to assess the role of N-terminal acidic residues in the interactions of actin with myosin in the contractile cycle. Cosedimentation experiments revealed an approximately 3-fold decrease in the binding ...

journal_title：Biochemistry

authors： Miller CJ,Wong WW,Bobkova E,Rubenstein PA,Reisler E

更新日期：1996-12-24 00:00:00

abstract：:Copper thiosemicarbazones cause considerable oxidative stress. This effect may be related to their cytotoxicity. In the present work, the chemical and cellular properties of a new ligand, pyridoxal thiosemicarbazone (H2T), and its copper(II) chelate (CuT) are assessed. CuT is toxic to cultured Ehrlich ascites tumor ce...

journal_title：Biochemistry

authors： Byrnes RW,Mohan M,Antholine WE,Xu RX,Petering DH

更新日期：1990-07-31 00:00:00

abstract：:The EcoRV restriction/modification system consists of two enzymes that recognize the DNA sequence GATATC. The EcoRV restriction endonuclease cleaves DNA at this site, but the DNA of Escherichia coli carrying the EcoRV system is protected from this reaction by the EcoRV methyltransferase. However, in vitro, the EcoRV n...

journal_title：Biochemistry

authors： Taylor JD,Goodall AJ,Vermote CL,Halford SE

更新日期：1990-12-04 00:00:00

abstract：:The three-dimensional solution structure of the repeating -PO4-6Gal beta 1-4Man alpha 1- disaccharide fragment of the lipophosphoglycan (LPG) derived from Leishmania donovani has been determined by use of a combination of homo- and heteronuclear NMR spin coupling constant measurements together with restrained molecula...

journal_title：Biochemistry

authors： Homans SW,Mehlert A,Turco SJ

更新日期：1992-01-28 00:00:00

abstract：:Phase-sensitive 2D 1H/1H COSY spectra can be used to identify the structures of individual pure specimens of the aminoglycoside antibiotic amikacin and its N-hemisuccinyl derivatives. However, even at 500 MHz the 2D chemical shift dispersion does not allow for unambiguous assignment of all cross-peaks. By use of 2D re...

journal_title：Biochemistry

authors： Andersen NH,Eaton HL,Nguyen KT,Hartzell C,Nelson RJ,Priest JH

更新日期：1988-04-19 00:00:00

abstract：:Beta-2-microglobulin (beta2m) deposits as amyloid fibrils in the musculoskeletal system of patients undergoing long-term dialysis treatment as a result of kidney failure. Previous work has shown that Cu(II) binding causes beta2m to organize into nativelike dimers and tetramers that precede amyloid formation. Cu(II) is...

journal_title：Biochemistry

authors： Srikanth R,Mendoza VL,Bridgewater JD,Zhang G,Vachet RW

更新日期：2009-10-20 00:00:00

abstract：:NADP(H) binding is essential for fast electron transfer through the flavoprotein domain of the fusion protein P450BM3. Here we characterize the interaction of NADP(H) with the oxidized and partially reduced enzyme and the effect of this interaction on the redox properties of flavin cofactors and electron transfer. Mea...

journal_title：Biochemistry

authors： Murataliev MB,Feyereisen R

更新日期：2000-10-17 00:00:00

abstract：:Posttranslational glycosylation is critical for biological function of many proteins, but its structural characterization is complicated by natural heterogeneity, multiple glycosylation sites, and different forms. Here, a top-down mass spectrometry (MS) characterization is applied to three constructs of the Fc segment...

journal_title：Biochemistry

authors： Fridriksson EK,Beavil A,Holowka D,Gould HJ,Baird B,McLafferty FW

更新日期：2000-03-28 00:00:00

abstract：:A minor fraction of plasma high-density lipoprotein (pre beta-1 HDL) has been shown to promote cholesterol efflux from peripheral cell membranes [Castro, G. R., & Fielding, C. J. (1988) Biochemistry 27, 25-29]. When isolated native plasma is incubated at 37 degrees C, this fraction is specifically decreased. On the ot...

journal_title：Biochemistry

authors： Miida T,Kawano M,Fielding CJ,Fielding PE

更新日期：1992-11-17 00:00:00

abstract：:Heme oxygenases convert heme to free iron, CO, and biliverdin. Saccharomyces cerevisiae and Candida albicans express putative heme oxygenases that are required for the acquisition of iron from heme, a critical process for fungal survival and virulence. The putative heme oxygenases Hmx1 and CaHmx1 from S. cerevisiae an...

journal_title：Biochemistry

authors： Kim D,Yukl ET,Moënne-Loccoz P,Montellano PR

更新日期：2006-12-12 00:00:00

abstract：:The effect of Ca2+ binding on the circular dichroism (CD) and 270-MHz proton nuclear magnetic resonance (NMR) spectra of brain-specific S-100b calcium binding protein has been examined at two pH values, 8.5 and 7.5. At pH 8.5, S-100b protein binds two Ca2+ per monomer with Kd values of 6 x 10(-5) and 2 x 10(-4) M, whe...

journal_title：Biochemistry

authors： Mani RS,Shelling JG,Sykes BD,Kay CM

更新日期：1983-03-29 00:00:00

abstract：:Combined quantum mechanical/molecular mechanical (QM/MM) simulations are carried out to analyze factors that dictate the proton transfer in carbonic anhydrase II (CAII), an enzyme that has been used as a prototypical example of long-range proton transfers in biomolecules. In contrast to the long-held conjecture in the...

journal_title：Biochemistry

authors： Riccardi D,König P,Guo H,Cui Q

更新日期：2008-02-26 00:00:00