Regulation of the concentration of pre beta high-density lipoprotein in normal plasma by cell membranes and lecithin-cholesterol acyltransferase activity.


:A minor fraction of plasma high-density lipoprotein (pre beta-1 HDL) has been shown to promote cholesterol efflux from peripheral cell membranes [Castro, G. R., & Fielding, C. J. (1988) Biochemistry 27, 25-29]. When isolated native plasma is incubated at 37 degrees C, this fraction is specifically decreased. On the other hand, the level of plasma pre beta-1 HDL is fully protected in the presence of even very low levels of fibroblasts, vascular smooth muscle cells, or macrophages. Blood cells were completely inactive in maintaining plasma pre beta-1 HDL levels in the absence of peripheral cells, even at the relatively high levels present in whole blood. The loss of pre beta-1 observed in isolated plasma was dependent upon lecithin-cholesterol acyltransferase (LCAT) activity. These data suggest that reverse cholesterol transport catalyzed by pre beta-1 HDL, and subsequent LCAT-mediated cholesterol esterification, is directly dependent upon the interaction between this HDL species and competent peripheral cells.






Miida T,Kawano M,Fielding CJ,Fielding PE




Has Abstract


1992-11-17 00:00:00












  • Overexpression and functional characterization of the extracellular domain of the human alpha1 glycine receptor.

    abstract::A novel truncated form (residues 1-214, with a randomized C-terminal tail) of the ligand-binding extracellular domain (ECD) of the human alpha1 glycine receptor (GlyR), with amino acids from the corresponding sequence of an acetylcholine binding protein (AChBP) substituted for two relatively hydrophobic membrane-proxi...


    pub_type: 杂志文章


    authors: Liu Z,Ramanoudjame G,Liu D,Fox RO,Jayaraman V,Kurnikova M,Cascio M

    更新日期:2008-09-16 00:00:00

  • Mechanism of inhibition of the class C beta-lactamase of Enterobacter cloacae P99 by phosphonate monoesters.

    abstract::The class C serine beta-lactamase of Enterobacter cloacae P99 was inhibited by a series of aryl methylphosphonate monoester monoanions. The effectiveness of these inhibitors was promoted by an acylamido substituent on the methyl group and a good leaving group at phosphorus. The former preference suggests that noncoval...


    pub_type: 杂志文章


    authors: Rahil J,Pratt RF

    更新日期:1992-06-30 00:00:00

  • Cardiac-specific Nkx2.5 homeodomain: conformational stability and specific DNA binding of Nkx2.5(C56S).

    abstract::The cardiac-specific Nkx2.5 homeodomain has been expressed as a 79-residue protein with the oxidizable Cys(56) replaced with Ser. The Nkx2.5 or Nkx2.5(C56S) homeodomain is 73% identical in sequence to and has the same NMR structure as the vnd (ventral nervous system defective)/NK-2 homeodomain of Drosophila when bound...


    pub_type: 杂志文章


    authors: Fodor E,Mack JW,Maeng JS,Ju JH,Lee HS,Gruschus JM,Ferretti JA,Ginsburg A

    更新日期:2005-09-20 00:00:00

  • Volume changes during enzyme reactions: indications of enzyme pulsation during fumarase catalysis.

    abstract::Overall activation volumes for multistep reactions are not usually pressure independent. The present investigation gives a quantitative description of this effect under Theory. Simple relations are obtained which can easily be applied to experimental data and which allow more insight into the dynamics of enzyme reacti...


    pub_type: 杂志文章


    authors: Butz P,Greulich KO,Ludwig H

    更新日期:1988-03-08 00:00:00

  • Inhibitor Bound Crystal Structures of Bacterial Nitric Oxide Synthase.

    abstract::Nitric oxide generated by bacterial nitric oxide synthase (NOS) increases the susceptibility of Gram-positive pathogens Staphylococcus aureus and Bacillus anthracis to oxidative stress, including antibiotic-induced oxidative stress. Not surprisingly, NOS inhibitors also improve the effectiveness of antimicrobials. Dev...


    pub_type: 杂志文章


    authors: Holden JK,Dejam D,Lewis MC,Huang H,Kang S,Jing Q,Xue F,Silverman RB,Poulos TL

    更新日期:2015-07-07 00:00:00

  • 31P NMR visibility and characterization of rat liver mitochondrial matrix adenine nucleotides.

    abstract::Compartmentation and NMR visibility of mitochondrial adenine nucleotides were quantitated in isolated rat liver mitochondria respiring on succinate and glutamate in vitro at 8 and 25 degrees C. Intra- and extramitochondrial nucleotides were discriminated by adding the chelator trans-1,2-diaminocyclohexane-N,N,N',N'-te...


    pub_type: 杂志文章


    authors: Hutson SM,Berkich D,Williams GD,LaNoue KF,Briggs RW

    更新日期:1989-05-16 00:00:00

  • Phosphorylation of calmodulin alters its potency as an activator of target enzymes.

    abstract::Previous work has shown that calmodulin (CaM) is constitutively phosphorylated in rat liver, probably by casein kinase II [Quadroni, M., James, P., and Carafoli, E. (1994) J. Biol. Chem. 269, 16116-16122]. A procedure is now described for the isolation of the phosphorylated forms of calmodulin (PCaM) free from CaM, si...


    pub_type: 杂志文章


    authors: Quadroni M,L'Hostis EL,Corti C,Myagkikh I,Durussel I,Cox J,James P,Carafoli E

    更新日期:1998-05-05 00:00:00

  • Local interactions drive the formation of nonnative structure in the denatured state of human alpha-lactalbumin: a high resolution structural characterization of a peptide model in aqueous solution.

    abstract::There are a small number of peptides derived from proteins that have a propensity to adopt structure in aqueous solution which is similar to the structure they possess in the parent protein. There are far fewer examples of protein fragments which adopt stable nonnative structures in isolation. Understanding how nonnat...


    pub_type: 杂志文章


    authors: Demarest SJ,Hua Y,Raleigh DP

    更新日期:1999-06-01 00:00:00

  • Carbon-13 nuclear magnetic resonance studies on the interaction of glycophorin with lecithin in reconstituted vesicles.

    abstract::Glycophorin, the MN blood group substance, is a major intrinsic glycoprotein in erythrocyte membranes. The interaction of glycophorin with phosphatidylcholine, 13C-labeled in specific positions in reconstituted unilamellar vesicles, was investigated by using the 13C NMR technique. 1-Palmitoyl-2-([14-13C]linoleoyl)-sn-...


    pub_type: 杂志文章


    authors: Utsumi H,Tunggal BD,Stoffel W

    更新日期:1980-05-27 00:00:00

  • Different binding modes of two flaviolin substrate molecules in cytochrome P450 158A1 (CYP158A1) compared to CYP158A2.

    abstract::Cytochrome P450 158A2 (CYP158A2) has been shown to catalyze an unusual oxidative C-C coupling reaction to polymerize flaviolin and form highly conjugated pigments (three isomers of biflaviolin and one triflaviolin) in Streptomyces coelicolor A3(2) which protect the soil bacterium from deleterious effects of UV irradia...


    pub_type: 杂志文章


    authors: Zhao B,Lamb DC,Lei L,Kelly SL,Yuan H,Hachey DL,Waterman MR

    更新日期:2007-07-31 00:00:00

  • Site selectivity of daunomycin.

    abstract::We have reexamined the binding properties of the antitumor drug daunomycin using double-helical oligonucleotides 16 base pairs long that were designed to contain preferred binding sites for the drug. The preferred sites are contained in a six base pair core which is flanked on the 5' and 3' ends by tracts of adenines....


    pub_type: 杂志文章


    authors: Roche CJ,Thomson JA,Crothers DM

    更新日期:1994-02-01 00:00:00

  • Reaction mechanism of the iron-sulfur flavoenzyme adenosine-5'-phosphosulfate reductase based on the structural characterization of different enzymatic states.

    abstract::The iron-sulfur flavoenzyme adenosine-5'-phosphosulfate (APS) reductase catalyzes a key reaction of the global sulfur cycle by reversibly transforming APS to sulfite and AMP. The structures of the dissimilatory enzyme from Archaeoglobus fulgidus in the reduced state (FAD(red)) and in the sulfite adduct state (FAD-sulf...


    pub_type: 杂志文章


    authors: Schiffer A,Fritz G,Kroneck PM,Ermler U

    更新日期:2006-03-07 00:00:00

  • NH2-terminal extensions on skin collagen from sheep with a genetic defect in conversion of procollagen into collagen.

    abstract::A modified form of procollagen was extracted with 10 M urea from the skin of lambs with dermatosparaxis, a disease which is produced by a genetic defect in the conversion of procollagen to collagen. The extracts contained little if any alpha1 and alpha2 chains of normal type I collagen, and instead they contained the ...


    pub_type: 杂志文章


    authors: Becker U,Timpl R

    更新日期:1976-06-29 00:00:00

  • Heme binding properties of glyceraldehyde-3-phosphate dehydrogenase.

    abstract::Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme that also functions in transcriptional regulation, oxidative stress, vesicular trafficking, and apoptosis. Because GAPDH is required for the insertion of cellular heme into inducible nitric oxide synthase [Chakravarti, R., et al. (2010) Proc. Natl...


    pub_type: 杂志文章


    authors: Hannibal L,Collins D,Brassard J,Chakravarti R,Vempati R,Dorlet P,Santolini J,Dawson JH,Stuehr DJ

    更新日期:2012-10-30 00:00:00

  • Kinetics of oxidative phosphorylation in Paracoccus denitrificans. 1. Mechanism of ATP synthesis at the active site(s) of F0F1-ATPase.

    abstract::(1) The rate of ATP synthesis during NADH-driven aerobic respiration has been measured in plasma membrane vesicles from Paracoccus denitrificans as a function of the concentration of the substrates, ADP and inorganic phosphate (Pi). In both cases, the response of the reaction to changes in the degree of saturation of ...


    pub_type: 杂志文章


    authors: Pérez JA,Ferguson SJ

    更新日期:1990-11-20 00:00:00

  • Effect of flap modifications on human FEN1 cleavage.

    abstract::The flap endonuclease, FEN1, plays a critical role in DNA replication and repair. Human FEN1 exhibits both a 5' to 3' exonucleolytic and a structure-specific endonucleolytic activity. On primer-template substrates containing an unannealed 5'-tail, or flap structure, FEN1 employs a unique mechanism to cleave at the poi...


    pub_type: 杂志文章


    authors: Bornarth CJ,Ranalli TA,Henricksen LA,Wahl AF,Bambara RA

    更新日期:1999-10-05 00:00:00

  • Interaction and localization of the retinitis pigmentosa protein RP2 and NSF in retinal photoreceptor cells.

    abstract::RP2 is a ubiquitously expressed protein encoded by a gene associated with X-linked retinitis pigmentosa (XLRP), a retinal degenerative disease that causes severe vision loss. Previous in vitro studies have shown that RP2 binds to ADP ribosylation factor-like 3 (Arl3) and activates its intrinsic GTPase activity, but th...


    pub_type: 杂志文章


    authors: Holopainen JM,Cheng CL,Molday LL,Johal G,Coleman J,Dyka F,Hii T,Ahn J,Molday RS

    更新日期:2010-09-07 00:00:00

  • Triple helical structures involving inosine: there is a penalty for promiscuity.

    abstract::Inosine has the ability to act as a "wild-card" binding nonspecifically to both A.T and G.C base pairs. This has obvious implications for the design of oligonucleotide site-directed probes. In this paper we present a series of oligonucleotides with a 5'pur9-pyr9-pyr9 motif which are designed to fold up sequentially in...


    pub_type: 杂志文章


    authors: Mills M,Völker J,Klump HH

    更新日期:1996-10-15 00:00:00

  • Kinetic and secondary structure analysis of Naegleria andersoni GIR1, a group I ribozyme whose putative biological function is site-specific hydrolysis.

    abstract::NanGIR1 is a catalytic element inserted in the P6 loop of a group I intron (NanGIR2) in the small subunit rRNA precursor of the protist Naegleria andersoni [Einvik, C., Decatur, W. A., Embley, T. M., Vogt, V. M., and Johansen, S. (1997) RNA 3, 710-720]. It catalyzes site-specific hydrolysis at an internal processing s...


    pub_type: 杂志文章


    authors: Jabri E,Aigner S,Cech TR

    更新日期:1997-12-23 00:00:00

  • Cooperative binding of R17 coat protein to RNA.

    abstract::The binding of the R17 coat protein to synthetic RNAs containing one or two coat protein binding sites was characterized by using nitrocellulose filter and gel-retention assays. RNAs with two available sites bound coat protein in a cooperative manner, resulting in a higher affinity and reduced sensitivity to pH, ionic...


    pub_type: 杂志文章


    authors: Witherell GW,Wu HN,Uhlenbeck OC

    更新日期:1990-12-18 00:00:00

  • G-protein-dependent cell surface dynamics of the human serotonin1A receptor tagged to yellow fluorescent protein.

    abstract::Serotonergic signaling appears to play a key role in the generation and modulation of various cognitive, behavioral, and developmental processes. The serotonin(1A) receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors and is the most extensively studied among the ...


    pub_type: 杂志文章


    authors: Pucadyil TJ,Kalipatnapu S,Harikumar KG,Rangaraj N,Karnik SS,Chattopadhyay A

    更新日期:2004-12-21 00:00:00

  • Identification of active-site residues of the pro-metastatic endoglycosidase heparanase.

    abstract::Heparanase is a beta-D-endoglucuronidase that cleaves heparan sulfate (HS) and has been implicated in many important physiological and pathological processes, including tumor cell metastasis, angiogenesis, and leukocyte migration. We report herein the identification of active-site residues of human heparanase. Using P...


    pub_type: 杂志文章


    authors: Hulett MD,Hornby JR,Ohms SJ,Zuegg J,Freeman C,Gready JE,Parish CR

    更新日期:2000-12-26 00:00:00

  • Structurally diverse chitolipooligosaccharide nod factors accumulate primarily in membranes of wild type Rhizobium leguminosarum biovar trifolii.

    abstract::The general view on Rhizobium chitolipooligosaccharides (CLOS) is that they are made in very low levels as diffusible molecules and are primarily secreted by the bacteria into the extracellular milieu where they interact with the host. However, the structural and predicted physicochemical properties of these amphiphil...


    pub_type: 杂志文章


    authors: Orgambide GG,Lee J,Hollingsworth RI,Dazzo FB

    更新日期:1995-03-21 00:00:00

  • Exchange of histidine spacing between Sp1 and GLI zinc fingers: distinct effect of histidine spacing-linker region on DNA binding.

    abstract::In the DNA recognition mode of C(2)H(2)-type zinc fingers, the finger-finger connection region, consisting of the histidine spacing (HX(3-5)H) and linker, would be important for determining the orientation of the zinc finger domains. To clarify the influence of spacing between two ligand histidines in the DNA binding,...


    pub_type: 杂志文章


    authors: Shiraishi Y,Imanishi M,Sugiura Y

    更新日期:2004-05-25 00:00:00

  • The EcoRI-DNA complex as a model for investigating protein-DNA interactions by atomic force microscopy.

    abstract::Atomic force microscopy (AFM) is a technique widely used to image protein-DNA complexes, and its application has now been extended to the measurements of protein-DNA binding constants and specificities. However, the spreading of the protein-DNA complexes on a flat substrate, generally mica, is required prior to AFM im...


    pub_type: 杂志文章


    authors: Sorel I,Piétrement O,Hamon L,Baconnais S,Cam EL,Pastré D

    更新日期:2006-12-12 00:00:00

  • Three-dimensional structure of the apo form of the N-terminal EGF-like module of blood coagulation factor X as determined by NMR spectroscopy and simulated folding.

    abstract::The three-dimensional structure of a 42-residue fragment containing the N-terminal EGF-like module of blood coagulation factor X was determined by means of 2D NMR spectroscopy and computer simulation. The spectroscopic data consisted of 370 NOE distances and 27 dihedral angle constraints. These were used to generate p...


    pub_type: 杂志文章


    authors: Ullner M,Selander M,Persson E,Stenflo J,Drakenberg T,Teleman O

    更新日期:1992-07-07 00:00:00

  • Separation and quantitation of intracellular forms of poliovirus RNA by agarose gel electrophoresis.

    abstract::Intracellular poliovirus-specific RNA species can be measured directly by electrophoresis of total cytoplasmic nucleic acids through 1% agarose gels, resulting in the separation of single- and double-stranded forms of poliovirus RNA from each other and from HeLa cell 28S ribosomal RNA. Single-stranded RNA molecules di...


    pub_type: 杂志文章


    authors: Hewlett MJ,Rozenblatt S,Ambros V,Baltimore D

    更新日期:1977-06-14 00:00:00

  • Functional groups at the catalytic site of BF1 adenosinetriphosphatase from Escherichia coli.

    abstract::The rates of inactivation of BF1 adenosinetriphosphatase (BF1-ATPase) from Escherichia coli by 7-chloro-4-nitro-2,1,3-benzoxadiazole, 1-fluoro-2,4-dinitrobenzene, 2,4,6-trinitrobenzenesulfonate, phenylglyoxal, and N,N'-dicyclohexylcarbodiimide have been measured in the presence and absence of various concentrations of...


    pub_type: 杂志文章


    authors: Ting LP,Wang JH

    更新日期:1982-01-19 00:00:00

  • Structure-activity relationships for the interaction of bovine pancreatic trypsin inhibitor with an intracellular site on a large conductance Ca(2+)-activated K(+) channel.

    abstract::Large conductance Ca(2+)-activated K(+) channels (BK(Ca)) contain an intracellular binding site for bovine pancreatic trypsin inhibitor (BPTI), a well-known inhibitor of various serine proteinase (SerP) enzymes. To investigate the structural basis of this interaction, we examined the activity of 11 BPTI mutants using ...


    pub_type: 杂志文章


    authors: Favre I,Moss GW,Goldenberg DP,Otlewski J,Moczydlowski E

    更新日期:2000-02-29 00:00:00

  • Substitution of Tyr254 with Phe at the active site of flavocytochrome b2: consequences on catalysis of lactate dehydrogenation.

    abstract::A role for Tyr254 in L-lactate dehydrogenation catalyzed by flavocytochrome b2 has recently been proposed on the basis of the known active-site structure and of studies that had suggested a mechanism involving the initial formation of a lactate carbanion [Lederer, F., & Mathews, F.S. (1987) in Flavins and Flavoprotein...


    pub_type: 杂志文章


    authors: Dubois J,Chapman SK,Mathews FS,Reid GA,Lederer F

    更新日期:1990-07-10 00:00:00