Cytochrome P-450 transfer from adrenocortical submitochondrial particles to liposome membranes.

abstract：:The light-induced QA-/QA FTIR difference spectrum of the photoreduction of the primary quinone (QA) in reaction centers (RCs) from Rhodobacter sphaeroides exhibits a set of complex differential bands between 1750 and 1715 cm(-1). Several of these features correspond in frequency to bands that bleach in the HA-/HA FTIR...

journal_title：Biochemistry

authors： Breton J,Nabedryk E,Allen JP,Williams JC

更新日期：1997-04-15 00:00:00

abstract：:Binding of mitochondrial cyclophilin (CyP) to the inner mitochondrial membrane is induced by treatment of mitochondria with thiol reagents or oxidative stress and correlates with a sensitization to [Ca2+] of the cyclosporin A-sensitive mitochondrial permeability transition pore (MTP) [Connern, C. P., & Halestrap, A. P...

journal_title：Biochemistry

authors： Connern CP,Halestrap AP

更新日期：1996-06-25 00:00:00

abstract：:The product of transesterification of phospholipid acyl chains and unesterified cholesterol (UC) by the enzyme lecithin:cholesterol acyltransferase (LCAT) is cholesteryl ester (CE). Activation of LCAT by apolipoprotein (apo) A-I on nascent (discoidal) high-density lipoproteins (HDL) is essential for formation of matur...

journal_title：Biochemistry

authors： Jones MK,Catte A,Li L,Segrest JP

更新日期：2009-12-01 00:00:00

abstract：:Cyanobacteriochromes (CBCRs) are photosensory proteins related to the red/far-red phytochromes. Like phytochromes, CBCRs use linear tetrapyrrole (bilin) chromophores covalently attached via a thioether linkage to a conserved Cys residue also found in plant and cyanobacterial phytochromes. Unlike almost all phytochrome...

journal_title：Biochemistry

authors： Rockwell NC,Martin SS,Lagarias JC

更新日期：2012-05-01 00:00:00

abstract：:ncd is a kinesin-related motor protein from Drosophila that moves in the opposite direction along microtubules to kinesin. To learn more about the ncd mechanism, ncd motor domain (R335-K700) was expressed in Escherichia coli and its enzymatic characteristics were studied. The ncd motor domain was purified from the cel...

journal_title：Biochemistry

authors： Shimizu T,Sablin E,Vale RD,Fletterick R,Pechatnikova E,Taylor EW

更新日期：1995-10-10 00:00:00

abstract：:The sequential solid-phase synthesis of a peptide analogue of bovine brain calmodulin calcium binding site III covering residues 81-113 of the natural sequence is described. Methionine-109 is replaced by a leucine residue to avoid complications in the synthesis and purification. In an attempt to relate the structure o...

journal_title：Biochemistry

authors： Reid RE

更新日期：1987-09-22 00:00:00

abstract：:The first positive evidence for the utilization of a direct C-6' ' oxidation/reduction mechanism by ADP-l-glycero-d-manno-heptose 6-epimerase is reported here. The epimerase (HldD or AGME, formerly RfaD) operates in the biosynthetic pathway of l-glycero-d-manno-heptose, which is a conserved sugar in the core region of...

journal_title：Biochemistry

authors： Morrison JP,Read JA,Coleman WG Jr,Tanner ME

更新日期：2005-04-19 00:00:00

abstract：:The three subunits I, II, and III of bovine procarboxypeptidase A separated by reversible dimethylmaleylation can reassociate to form the reconstituted complexes I + II, I + III, and I + II + III. Since the association II + III is not possible, subunit I appears to play a central role in the formation of the complex. ...

journal_title：Biochemistry

authors： Puigserver A,Desnuelle P

更新日期：1977-05-31 00:00:00

abstract：:HSC70 interacting protein (HIP) is an essential cytoplasmic cochaperone involved in the regulation of HSC70 chaperone activity and the maturation of progesterone receptor. To determine the quaternary structure and the gross conformation of the protein in solution, a wide array of biochemical and biophysical techniques...

journal_title：Biochemistry

authors： Velten M,Villoutreix BO,Ladjimi MM

更新日期：2000-01-18 00:00:00

abstract：:Escherichia coli class Ib ribonucleotide reductase (RNR) converts nucleoside 5'-diphosphates to deoxynucleoside 5'-diphosphates in iron-limited and oxidative stress conditions. We have recently demonstrated in vitro that this RNR is active with both diferric-tyrosyl radical (Fe(III)(2)-Y(•)) and dimanganese(III)-Y(•) ...

journal_title：Biochemistry

authors： Cotruvo JA,Stubbe J

更新日期：2011-03-15 00:00:00

abstract：:Ornithine decarboxylase (ODC) catalyzes the first committed step in the biosynthesis of polyamines, and it has been identified as a drug target for the treatment of African sleeping sickness, caused by Trypanosoma brucei. ODC is a pyridoxal 5'-phosphate (PLP) dependent enzyme and an obligate homodimer. X-ray structura...

journal_title：Biochemistry

authors： Jackson LK,Brooks HB,Osterman AL,Goldsmith EJ,Phillips MA

更新日期：2000-09-19 00:00:00

abstract：:His216 is a well-conserved residue in pyruvate carboxylases and, on the basis of structures of the enzyme, appears to have a role in the binding of MgATP, forming an interaction with the 3'-hydroxyl group of the ribose ring. Mutation of this residue to asparagine results in a 9-fold increase in the Km for MgATP in its...

journal_title：Biochemistry

authors： Adina-Zada A,Jitrapakdee S,Wallace JC,Attwood PV

更新日期：2014-02-18 00:00:00

abstract：:Adenylosuccinate synthetase catalyzes the first committed step in the de novo biosynthesis of AMP, coupling L-aspartate and IMP to form adenylosuccinate. Km values of IMP and 2'-deoxy-IMP are nearly identical with each substrate supporting comparable maximal velocities. Nonetheless, the Km value for L-aspartate and th...

journal_title：Biochemistry

authors： Iancu CV,Zhou Y,Borza T,Fromm HJ,Honzatko RB

更新日期：2006-09-26 00:00:00

abstract：:Sedimentation equilibrium and sedimentation velocity measurements demonstrate that turkey gizzard caldesmon is an elongated molecule of molecular mass 75 +/- 2 kDa. The frictional ratio (2.14) is consistent with a prolate ellipsoid of axial ratio 24, corresponding to an apparent length and width of 516 and 21.5 A, res...

journal_title：Biochemistry

authors： Malencik DA,Ausio J,Byles CE,Modrell B,Anderson SR

更新日期：1989-10-03 00:00:00

abstract：:We have measured the temperature dependence of the oxygen-binding isotherms of human and bovine hemoglobin at pH 9.0 in 0.1 M borate buffer. In both hemoglobins the ionization of the Bohr protons is finished at this pH; therefore, their heat does not interfere with the measurements. Two sets of curves have been obtain...

journal_title：Biochemistry

authors： Bucci E,Fronticelli C,Gryczynski Z

更新日期：1991-04-02 00:00:00

abstract：:Influenza virus gains access to the cytoplasm of its host cell by means of a fusion event between viral and host cell membrane. Fusion is mediated by the envelope glycoprotein hemagglutinin (HA) and is triggered by low pH. To learn how many hemagglutinin trimers are necessary to cause membrane fusion, we have used two...

journal_title：Biochemistry

authors： Ellens H,Bentz J,Mason D,Zhang F,White JM

更新日期：1990-10-16 00:00:00

abstract：:The interaction of profilin and non-muscle beta,gamma-actin prepared from bovine spleen has been investigated under physiologic ionic conditions. Profilin binding to actin decreases the affinity of actin for MgADP and MgATP by about 65- and 13-fold, respectively. Kinetic measurements indicate that profilin binding to ...

journal_title：Biochemistry

authors： Kinosian HJ,Selden LA,Gershman LC,Estes JE

更新日期：2000-10-31 00:00:00

abstract：:In the present study we establish that specific members of the Sp1 family of transcription factors (Sp1 and Sp3) bind to all six GC-rich motifs (elements 1-6) present in the proximal promoter of the human cell cycle inhibitor p21(WAF-1/Cip1) gene. Competition analysis showed that Sp1 and Sp3 bound with high affinity t...

journal_title：Biochemistry

authors： Koutsodontis G,Moustakas A,Kardassis D

更新日期：2002-10-22 00:00:00

abstract：:The natural ATPase inhibitor (IF1) from beef heart mitochondria was labeled with phenyl (14C)isothiocyanate [(14C)PITC]. Chemical labeling by (14C)PITC does not modify the inhibitory properties of IF1, provided the number of residues of (14C)PITC bound per molecule of IF1 is lower than five to six, which corresponds t...

journal_title：Biochemistry

authors： Klein G,Satre M,Dianoux AC,Vignais PV

更新日期：1980-06-24 00:00:00

abstract：:Adeno-associated virus (AAV) nonstructural proteins Rep78 and Rep68 carry out all DNA transactions that regulate the AAV life cycle. They share two multifunctional domains: an N-terminal origin binding/nicking domain (OBD) from the HUH superfamily and a SF3 helicase domain. A short linker of ∼20 amino acids that is cr...

journal_title：Biochemistry

authors： Musayev FN,Zarate-Perez F,Bardelli M,Bishop C,Saniev EF,Linden RM,Henckaerts E,Escalante CR

更新日期：2015-09-29 00:00:00

abstract：:A cationic, copper(II)-containing ligand, derived from bulky 5,10,15,20-tetrakis(N-methylpyridinium-4-yl)porphyrin, Cu(T4), and two sterically friendlier forms, [trans-5,15-di(N-methylpyridinium-4-yl)porphyrinato]copper(II), Cu(tD4), and [cis-5,10-di(N-methylpyridinium-4-yl)porphyrinato]copper(II), Cu(cD4), bind to DN...

journal_title：Biochemistry

authors： Briggs BN,Gaier AJ,Fanwick PE,Dogutan DK,McMillin DR

更新日期：2012-09-25 00:00:00

abstract：:The fibronectin receptor (FnR), the vitronectin receptor (VnR), and the platelet membrane glycoprotein (GP) IIb-IIIa complex are members of a family of cell adhesion receptors, which consist of noncovalently associated alpha- and beta-subunits. The present study was designed to compare the cDNA-derived protein sequenc...

journal_title：Biochemistry

authors： Fitzgerald LA,Poncz M,Steiner B,Rall SC Jr,Bennett JS,Phillips DR

更新日期：1987-12-15 00:00:00

abstract：:The reduction of substrates catalyzed by nitrogenase normally requires nucleotide-dependent Fe protein delivery of electrons to the MoFe protein, which contains the active site FeMo cofactor. Here, it is reported that independent substitution of three amino acids (β-98(Tyr→His), α-64(Tyr→His), and β-99(Phe→His)) locat...

journal_title：Biochemistry

authors： Danyal K,Rasmussen AJ,Keable SM,Inglet BS,Shaw S,Zadvornyy OA,Duval S,Dean DR,Raugei S,Peters JW,Seefeldt LC

更新日期：2015-04-21 00:00:00

abstract：:Several 3-halo-3-(1-haloalkyl)-1(3H)-isobenzofuranones, 3-(1-haloalkylidene)-1(3H)-isobenzofuranones, and 3-bromomethyl-1H-2-benzopyran-1-ones containing masked halo ketone functional groups were synthesized and tested as inhibitors of several serine proteases including human leukocyte (HL) elastase and cathepsin G. W...

journal_title：Biochemistry

authors： Hemmi K,Harper JW,Powers JC

更新日期：1985-04-09 00:00:00

abstract：:The presence and subcellular localization of UDP-Gal:glucosylceramide beta 1----4galactosyltransferase (GalT-2) was investigated in rat liver. For this purpose, purified Golgi apparatus, endoplasmic reticulum, and plasma membrane fractions were prepared from the liver and used as the enzyme source for detecting GalT-2...

journal_title：Biochemistry

authors： Trinchera M,Fiorilli A,Ghidoni R

更新日期：1991-03-12 00:00:00

abstract：:The topography of the photosynthetic reaction center (RC) polypeptides (H, M, and L) was investigated by proteolysis and radioiodination of membrane vesicles isolated from Rhodopseudomonas sphaeroides. Chromatophores, obtained from French-pressed cell lysates, are closed vesicles' and oriented inside out with respect ...

journal_title：Biochemistry

authors： Bachmann RC,Gillies K,Takemoto JY

更新日期：1981-08-04 00:00:00

abstract：:The dependence of the activity of bovine Cu,Zn superoxide dismutase on pH and ionic strength was extensively investigated in the ranges of pH 7.4-pH 12.3 and of ionic strength of 0.02-0.25 M. The results obtained indicate that two positively charged groups having pK values of approximately 10.1 and 10.8 are involved i...

journal_title：Biochemistry

authors： Argese E,Viglino P,Rotilio G,Scarpa M,Rigo A

更新日期：1987-06-02 00:00:00

abstract：:The solution structure of the actinorhodin acyl carrier protein (act apo-ACP) from the polyketide synthase (PKS) of Streptomyces coelicolor A3(2) has been determined using 1H NMR spectroscopy, representing the first polyketide synthase component for which detailed structural information has been obtained. Twenty-four ...

journal_title：Biochemistry

authors： Crump MP,Crosby J,Dempsey CE,Parkinson JA,Murray M,Hopwood DA,Simpson TJ

更新日期：1997-05-20 00:00:00

abstract：:The complete amino acid sequence of the major component myoglobin from the dwarf sperm whale, Kogia simus, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequenator. Three easily separable peptides were obtained by cleaving the protein at its two...

journal_title：Biochemistry

authors： Dwulet FE,Jones BN,Lehman LD,Gurd FR

更新日期：1977-03-08 00:00:00

abstract：:The abnormal isoform of the scrapie prion protein PrPSc is both a host-derived protein and a component of the infectious agent causing scrapie. PrPSc and the normal cellular isoform PrPC have different physical properties that apparently arise from a posttranslational event. Both PrP isoforms are covalently modified a...

journal_title：Biochemistry

authors： Stahl N,Borchelt DR,Prusiner SB

更新日期：1990-06-05 00:00:00