Radiolabeling of natural adenosine triphosphatase inhibitor with phenyl (14C)isothiocyanate and study of its interaction with mitochondrial adenosine triphosphatase. Localization of inhibitor binding sites and stoichiometry of binding.

abstract：:The potential of the frequently encountered (βα)8-barrel fold to acquire new functions was tested by an approach combining random mutagenesis and selection in vivo. For this purpose, the genes encoding 52 different phosphate-binding (βα)8-barrel proteins were subjected to error-prone PCR and cloned into an expression ...

journal_title：Biochemistry

authors： Rohweder B,Lehmann G,Eichner N,Polen T,Rajendran C,Ruperti F,Linde M,Treiber T,Jung O,Dettmer K,Meister G,Bott M,Gronwald W,Sterner R

更新日期：2019-10-15 00:00:00

abstract：:PsbO, the photosystem II manganese stabilizing protein, contains an aspartate residue [Asp157 (spinach numbering)], which is highly conserved in eukaryotic and prokaryotic PsbOs. The homology model of the PSII-bound conformation of spinach PsbO presented here positions Asp157 in the large flexible loop of the protein....

journal_title：Biochemistry

authors： Popelkova H,Commet A,Yocum CF

更新日期：2009-12-22 00:00:00

abstract：:Stabilization of the plasma membrane Ca-ATPase (PMCA) in an inactive conformation upon oxidation of multiple methionines in the calcium regulatory protein calmodulin (CaM) is part of an adaptive cellular response to minimize ATP utilization and the generation of reactive oxygen species (ROS) under conditions of oxidat...

journal_title：Biochemistry

authors： Chen B,Mayer MU,Squier TC

更新日期：2005-03-29 00:00:00

abstract：:The N-terminal 17% of apolipoprotein B (apoB-17) is secreted lipid-poor while apoB-41 particles are secreted with a triacylglycerol (TAG)-rich core. Thus, the sequence between apoB-17 and apoB-41 is necessary for the assembly of TAG-rich lipoproteins. To delineate this region, C127 cells were permanently transfected t...

journal_title：Biochemistry

authors： Carraway M,Herscovitz H,Zannis V,Small DM

更新日期：2000-08-15 00:00:00

abstract：:UDP-GlcNAc 2-epimerase/ManNAc kinase (GNE) catalyzes the first two committed steps in sialic acid synthesis. In addition to the three previously described human GNE isoforms (hGNE1-hGNE3), our database and polymerase chain reaction analysis yielded five additional human isoforms (hGNE4-hGNE8). hGNE1 is the ubiquitousl...

journal_title：Biochemistry

authors： Yardeni T,Choekyi T,Jacobs K,Ciccone C,Patzel K,Anikster Y,Gahl WA,Kurochkina N,Huizing M

更新日期：2011-10-18 00:00:00

abstract：:The five-membered nitrogen plus heteroatom rings known as azolines or in their oxidized form as azoles are very common in natural products and drugs. The oxidation of thiazoline to thiazole in the cyanobactin class of natural products is one of the several important transformations that are known to alter the biologic...

journal_title：Biochemistry

authors： Gao S,Ge Y,Bent AF,Schwarz-Linek U,Naismith JH

更新日期：2018-10-16 00:00:00

abstract：:Structural properties of heparan sulfate preparations from hog mucosa and beef lung sources were obtained by application of Smith degradation and nitrous acid reactions. Products formed by these reactions indicated that most of the iduronic acid present in these mucopolysaccharides is ester sulfated, whereas N-sulfate...

journal_title：Biochemistry

authors： Cifonelli JA,King JA

更新日期：1977-05-17 00:00:00

abstract：:Mutagenesis of Escherichia coli manganese superoxide dismutase (MnSD) demonstrates involvement of the strictly conserved gateway tyrosine (Y34) in exogenous ligand interactions. Conservative replacement of this residue by phenylalanine (Y34F) affects the pH sensitivity of the active-site metal ion and perturbs ligand ...

journal_title：Biochemistry

authors： Whittaker MM,Whittaker JW

更新日期：1997-07-22 00:00:00

abstract：:The structures of beta class carbonic anhydrases (beta-CAs) determined so far fall into two distinct subclasses based on the observed coordination of the catalytic zinc (Zn2+) ion. The subclass of beta-CAs that coordinate Zn2+ tetrahedrally with four protein-derived ligands is represented by the structures of ortholog...

journal_title：Biochemistry

authors： Cronk JD,Rowlett RS,Zhang KY,Tu C,Endrizzi JA,Lee J,Gareiss PC,Preiss JR

更新日期：2006-04-11 00:00:00

abstract：:The manganese transport regulator (MntR) of Bacillus subtilis is activated by Mn(2+) to repress transcription of genes encoding transporters involved in the uptake of manganese. MntR is also strongly activated by cadmium, both in vivo and in vitro, but it is poorly activated by other metal cations, including calcium a...

journal_title：Biochemistry

authors： Kliegman JI,Griner SL,Helmann JD,Brennan RG,Glasfeld A

更新日期：2006-03-21 00:00:00

abstract：:To identify the specific component(s) in the target membrane involved in fusion of vesicular stomatitis virus (VSV), we examined the interaction of the virus with human erythrocyte membranes with asymmetric and symmetric bilayer distributions of phospholipids. Fusion was monitored spectrofluorometrically by the octade...

journal_title：Biochemistry

authors： Herrmann A,Clague MJ,Puri A,Morris SJ,Blumenthal R,Grimaldi S

更新日期：1990-05-01 00:00:00

abstract：:The lateral and rotational dynamics of phosphoinositides and their interactions with proteins were characterized using pyrene-labeled lipid analogues. In these systems, the collision frequency of pyrene-labeled lipids was studied by monitoring the monomeric pyrene fluorescence yield as a function of their mole fractio...

journal_title：Biochemistry

authors： Pap EH,Hanicak A,van Hoek A,Wirtz KW,Visser AJ

更新日期：1995-07-18 00:00:00

abstract：:Human S100A7 (psoriasin) is a metal-chelating protein expressed by epithelial cells. It is a 22-kDa homodimer with two EF-hand domains per subunit and two transition-metal-binding His3Asp sites at the dimer interface. Each subunit contains two cysteine residues that can exist as free thiols (S100A7red) or as an intram...

journal_title：Biochemistry

authors： Cunden LS,Brophy MB,Rodriguez GE,Flaxman HA,Nolan EM

更新日期：2017-10-31 00:00:00

abstract：:Casein kinase II (CKII) is a ubiquitous protein kinase, found predominantly in cell nuclei, which has two subunits in a tetrameric alpha 2 beta 2 or alpha alpha' beta 2 conformation. The catalytic center is present in the alpha subunit which is active by itself while beta is a regulatory subunit that can greatly enhan...

journal_title：Biochemistry

authors： Hinrichs MV,Jedlicki A,Tellez R,Pongor S,Gatica M,Allende CC,Allende JE

更新日期：1993-07-20 00:00:00

abstract：:A novel phosphotriesterase was recently discovered and purified from Sphingobium sp. TCM1 (Sb-PTE) and shown to catalyze the hydrolysis of a broad spectrum of organophosphate esters with a catalytic efficiency that exceeds 10(6) M(-1) s(-1) for the hydrolysis of triphenyl phosphate. The enzyme was crystallized and the...

journal_title：Biochemistry

authors： Mabanglo MF,Xiang DF,Bigley AN,Raushel FM

更新日期：2016-07-19 00:00:00

abstract：:Homoisocitrate dehydrogenase (HIcDH, 3-carboxy-2-hydroxyadipate dehydrogenase) catalyzes the fourth reaction of the alpha-aminoadipate pathway for lysine biosynthesis, the conversion of homoisocitrate to alpha-ketoadipate using NAD as an oxidizing agent. A chemical mechanism for HIcDH is proposed on the basis of the p...

journal_title：Biochemistry

authors： Lin Y,Volkman J,Nicholas KM,Yamamoto T,Eguchi T,Nimmo SL,West AH,Cook PF

更新日期：2008-04-01 00:00:00

abstract：:High-pressure 31P NMR was used for the first time to investigate the effects of pressure on the structure and dynamics of the phosphocholine headgroup in pure 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) multilamellar aqueous dispersions and in DPPC bilayers containing the positively charged form of the local an...

journal_title：Biochemistry

authors： Peng X,Jonas J

更新日期：1992-07-21 00:00:00

abstract：:Human placental lipocortin is a high-affinity substrate for rat brain protein kinase C in vitro with phosphorylation occurring on serine and threonine residues in a ratio of approximately 2 to 1. Comparison of the ability of various N-terminal-truncated derivatives of lipocortin to serve as phosphorylation substrates,...

journal_title：Biochemistry

authors： Schlaepfer DD,Haigler HT

更新日期：1988-06-14 00:00:00

abstract：:A stereochemical analysis has been carried out on two vinylpyruvate hydratases (VPH), which convert 2-hydroxy-2,4-pentadienoate to 2-keto-4S-hydroxypentanoate in meta-fission pathways. Bacterial strains with this pathway can use aromatic compounds as sole sources of energy and carbon. The analysis was carried out usin...

journal_title：Biochemistry

authors： Johnson WH Jr,Stack TM,Taylor SM,Burks EA,Whitman CP

更新日期：2016-07-26 00:00:00

abstract：:Formylglycinamide ribonucleotide amidotransferase (FGAR-AT) catalyzes the ATP- and glutamine-dependent formation of formylglycinamidine ribonucleotide, ADP, P(i), and glutamate in the fourth step of de novo purine biosynthesis. Like all amidotransferases (ATs), FGAR-AT is proposed to channel ammonia between a glutamin...

journal_title：Biochemistry

authors： Hoskins AA,Anand R,Ealick SE,Stubbe J

更新日期：2004-08-17 00:00:00

abstract：:Hypoxic respiratory diseases or hypoxia exposures are frequently accompanied by glucose intolerance and impaired nitric oxide (NO) availability. However, the molecular mechanism responsible for impaired NO production and insulin resistance (IR) during hypoxia remains obscure. In this study, we investigated the possibl...

journal_title：Biochemistry

authors： Singh B,Biswas I,Garg I,Sugadev R,Singh AK,Dey S,Khan GA

更新日期：2014-01-14 00:00:00

abstract：:Amyloid fibrils have been classically defined as linear, nonbranched polymeric proteins with a cross beta-sheet structure and the ability to alter the optical properties of the amyloid-specific dye Congo Red. Mounting evidence suggests that soluble oligomeric peptide assemblies approximately 2-20 nm in diameter are cr...

journal_title：Biochemistry

authors： Moore RA,Hayes SF,Fischer ER,Priola SA

更新日期：2007-06-19 00:00:00

abstract：:Escherichia coli, Clostridium sticklandii, and Methanococcus vannielii synthesize 75Se-labeled amino acid transfer ribonucleic acids [( 75Se]tRNAs) when grown with low levels (approximately equal to 1 microM) of 75SeO32-. When E. coli [75Se]tRNA was digested to nucleosides and analyzed by reversed-phase high-performan...

journal_title：Biochemistry

authors： Wittwer AJ,Tsai L,Ching WM,Stadtman TC

更新日期：1984-09-25 00:00:00

abstract：:The rate of the reaction between p-nitroperoxybenzoic acid and cytochrome c peroxidase (CcP) has been investigated as a function of pH and ionic strength. The pH dependence of the reaction between CcP and peracetic acid has also been determined. The rate of the reactions are influenced by two heme-linked ionizations i...

journal_title：Biochemistry

authors： Palamakumbura AH,Foshay MC,Vitello LB,Erman JE

更新日期：1999-11-23 00:00:00

abstract：:The existence of bacterial K(+)/H(+) antiporters that prevent the overaccumulation of potassium in the cytoplasm was predicted by Peter Mitchell almost 50 years ago. The importance of K(+)/H(+) antiport for bacterial physiology is widely recognized, but its molecular mechanisms remain underinvestigated. Here, we demon...

journal_title：Biochemistry

authors： Resch CT,Winogrodzki JL,Patterson CT,Lind EJ,Quinn MJ,Dibrov P,Häse CC

更新日期：2010-03-23 00:00:00

abstract：:The melanocortin-4 receptor (MC4R) is involved in regulating energy homeostasis and is a potential therapeutic target for obesity and cachexia. Molecular interactions between peptide ligands and MC4R have been studied in detail. Less is known regarding the role of these interactions in the mechanism of MC4R activation...

journal_title：Biochemistry

authors： Fleck BA,Ling N,Chen C

更新日期：2007-09-18 00:00:00

abstract：:The structural features of a chemically modified DNA template strand may promote error-prone DNA synthesis during replication. The resulting higher incidence of mutations, in turn, can eventually lead to tumor initiation. Structural insights into this process can be monitored by studying chemically modified base adduc...

journal_title：Biochemistry

authors： Cosman M,Hingerty BE,Geacintov NE,Broyde S,Patel DJ

更新日期：1995-11-21 00:00:00

abstract：:The effects of site-directed mutagenesis were used to explore the role of residues in M4 on the apparent Ki of a selective, K+-competitive inhibitor of the gastric H+,K+ ATPase, SCH28080. A double transfection expression system is described, utilizing HEK293 cells and separate plasmids encoding the alpha and beta subu...

journal_title：Biochemistry

authors： Munson KB,Lambrecht N,Sachs G

更新日期：2000-03-21 00:00:00

abstract：:A small metal-binding protein (SmbP) with no known similarity to other proteins in current databases was isolated and characterized from the periplasm of Nitrosomonas europaea. The primary structure of this small (9.9 kDa) monomeric protein is characterized by a series of 10 repeats of a seven amino acid motif and an ...

journal_title：Biochemistry

authors： Barney BM,LoBrutto R,Francisco WA

更新日期：2004-09-07 00:00:00

abstract：:Epidermal growth factor receptor (EGFR) exemplifies the family of receptor tyrosine kinases that mediate numerous cellular processes, including growth, proliferation, and differentiation. Moreover, gene amplification and EGFR mutations have been identified in a number of human malignancies, making this receptor an imp...

journal_title：Biochemistry

authors： Truong TH,Carroll KS

更新日期：2012-12-18 00:00:00