Localization in the Golgi apparatus of rat liver UDP-Gal:glucosylceramide beta 1----4galactosyltransferase.


:The presence and subcellular localization of UDP-Gal:glucosylceramide beta 1----4galactosyltransferase (GalT-2) was investigated in rat liver. For this purpose, purified Golgi apparatus, endoplasmic reticulum, and plasma membrane fractions were prepared from the liver and used as the enzyme source for detecting GalT-2. A pure Golgi apparatus, highly enriched in many glycosyltransferases, was the only fraction where GalT-2 was measurable. The reaction product formation rate under appropriate assay conditions, which requires high detergent concentration and Mn2+, was low but comparable with that of other glycosyltransferases. The product formation was stimulated by exogenously added acceptor GlcCer, donor UDP-Gal, and Golgi protein. The reaction product was a single spot that was identified by chromatographic behavior, sensitivity to beta-galactosidase, and permethylation studies as Gal beta 1----4Glc beta 1----1'Cer (lactosylceramide). A metabolic experiment, performed by determining the glycosphingolipids which became radioactive in the above subcellular fractions prepared from the liver of animals treated with glucose-labeled glucosylceramide, further indicated that the in vivo glycosylation of glucosylceramide takes place in the Golgi apparatus.






Trinchera M,Fiorilli A,Ghidoni R




Has Abstract


1991-03-12 00:00:00












  • Use of azidobestatin as a photoaffinity label to identify the active site peptide of leucine aminopeptidase.

    abstract::Aminopeptidases catalyze the hydrolysis of amino acid residues from the amino terminus of peptide substrates. They are found in most cells and tissues, and their activity has been implicated in myriad fundamental biochemical and physiological processes. Nevertheless, little is known about the structure of the aminopep...


    pub_type: 杂志文章


    authors: Taylor A,Peltier CZ,Jahngen EG Jr,Laxman E,Szewczuk Z,Torre FJ

    更新日期:1992-04-28 00:00:00

  • Structural dynamics of soluble chloride intracellular channel protein CLIC1 examined by amide hydrogen-deuterium exchange mass spectrometry.

    abstract::Chloride intracellular channel protein 1 (CLIC1) functions as an anion channel in plasma and nuclear membranes when its soluble monomeric form converts to an integral-membrane form. The transmembrane region of CLIC1 is located in its thioredoxin-like domain 1, but the mechanism whereby the protein converts to its memb...


    pub_type: 杂志文章


    authors: Stoychev SH,Nathaniel C,Fanucchi S,Brock M,Li S,Asmus K,Woods VL Jr,Dirr HW

    更新日期:2009-09-08 00:00:00

  • Peptoid-Substituted Hybrid Antimicrobial Peptide Derived from Papiliocin and Magainin 2 with Enhanced Bacterial Selectivity and Anti-inflammatory Activity.

    abstract::Antimicrobial peptides (AMPs) are important components of the host innate immune system. Papiliocin is a 37-residue AMP purified from larvae of the swallowtail butterfly Papilio xuthus. Magainin 2 is a 23-residue AMP purified from the skin of the African clawed frog Xenopus laevis. We designed an 18-residue hybrid pep...


    pub_type: 杂志文章


    authors: Shin A,Lee E,Jeon D,Park YG,Bang JK,Park YS,Shin SY,Kim Y

    更新日期:2015-06-30 00:00:00

  • Structural and Mutagenesis Studies Evince the Role of the Extended Protuberant Domain of Ribosomal Protein uL10 in Protein Translation.

    abstract::The lateral stalk of ribosomes constitutes the GTPase-associated center and is responsible for recruiting translation factors to the ribosomes. The eukaryotic stalk contains a P-complex, in which one molecule of uL10 (formerly known as P0) protein binds two copies of P1/P2 heterodimers. Unlike bacterial uL10, eukaryot...


    pub_type: 杂志文章


    authors: Choi KA,Yang L,Lee KM,Yu CW,Banfield DK,Ito K,Uchiumi T,Wong KB

    更新日期:2019-09-10 00:00:00

  • Mechanistic investigations of a ribozyme derived from the Tetrahymena group I intron: insights into catalysis and the second step of self-splicing.

    abstract::Self-splicing of Tetrahymena pre-rRNA proceeds in two consecutive phosphoryl transesterification steps. One major difference between these steps is that in the first an exogenous guanosine (G) binds to the active site, while in the second the 3'-terminal G414 residue of the intron binds. The first step has been extens...


    pub_type: 杂志文章


    authors: Mei R,Herschlag D

    更新日期:1996-05-07 00:00:00

  • Resonance Raman studies of the flavin and iron-sulfur centers of milk xanthine oxidase.

    abstract::Resonance Raman spectroscopy has been used to study milk xanthine oxidase, an enzyme containing molybdenum, binuclear iron-sulfur clusters, and FAD as cofactors. The contribution of FAD dominates the resonance Raman spectrum at frequencies above 500 cm-1. As expected, no bands assignable to FAD are observed in deflavo...


    pub_type: 杂志文章


    authors: Willis LJ,Loehr TM

    更新日期:1985-05-21 00:00:00

  • Duplex oligodeoxyribonucleotides cross-linked by mitomycin C at a single site: synthesis, properties, and cross-link reversibility.

    abstract::Oligodeoxyribonucleotides cross-linked by reductively activated mitomycin C (MC) were prepared and purified for the first time. The cross-linked products were structurally characterized by nucleoside and MC-nucleoside adduct analysis. Optimal conditions were established for the cross-linking reaction, resulting in hig...


    pub_type: 杂志文章


    authors: Borowy-Borowski H,Lipman R,Chowdary D,Tomasz M

    更新日期:1990-03-27 00:00:00

  • The sigma enigma: in vitro/in silico site-directed mutagenesis studies unveil σ1 receptor ligand binding.

    abstract::The σ1 receptor is an integral membrane protein that shares no homology with other receptor systems, has no unequivocally identified natural ligands, but appears to play critical roles in a wide variety of cell functions. While the number of reports of the possible functions of the σ1 receptor is increasing, almost no...


    pub_type: 杂志文章


    authors: Brune S,Schepmann D,Klempnauer KH,Marson D,Dal Col V,Laurini E,Fermeglia M,Wünsch B,Pricl S

    更新日期:2014-05-13 00:00:00

  • The C2B domain of synaptotagmin I is a Ca2+-binding module.

    abstract::Synaptotagmin I is a synaptic vesicle protein that contains two C(2) domains and acts as a Ca(2+) sensor in neurotransmitter release. The Ca(2+)-binding properties of the synaptotagmin I C(2)A domain have been well characterized, but those of the C(2)B domain are unclear. The C(2)B domain was previously found to pull ...


    pub_type: 杂志文章


    authors: Ubach J,Lao Y,Fernandez I,Arac D,Südhof TC,Rizo J

    更新日期:2001-05-22 00:00:00

  • Effects of DNA structure on oxopropenylation by the endogenous mutagens malondialdehyde and base propenal.

    abstract::Malondialdehyde (MDA) and nucleobase propenals can transfer oxopropenyl groups to guanine residues of DNA to yield pyrimodopurinone (M(1)G) adducts. The DNA structural requirements for reaction with alpha,beta-unsaturated aldehydes were explored. We found that single-stranded DNA is more sensitive to oxopropenylation ...


    pub_type: 杂志文章


    authors: Plastaras JP,Dedon PC,Marnett LJ

    更新日期:2002-04-16 00:00:00

  • Calcium modulates the photoassembly of photosystem II (Mn)4-clusters by preventing ligation of nonfunctional high-valency states of manganese.

    abstract::The requirement for Ca2+ in the Mn(2+)-dependent photoactivation of oxygen evolution was re-evaluated using 17 kDa/24 kDa-less photosystem II (PSII) membranes depleted of (Mn)4-clusters by NH2OH extraction. At optimum conditions (1 mM Mn2+/10 microM 2,6-dichlorphenolindophenol (DCIP)/20 mM Ca2+), the light-induced inc...


    pub_type: 杂志文章


    authors: Chen C,Kazimir J,Cheniae GM

    更新日期:1995-10-17 00:00:00

  • Thermodynamics of transient conformations in the folding pathway of barnase: reorganization of the folding intermediate at low pH.

    abstract::New classes of small proteins have recently been found that refold rapidly with two-state kinetics from a substantially unfolded conformation ("U") and without the accumulation of a folding intermediate. Barnase, on the other hand, is representative of a class of proteins that display multistate kinetics and refold fr...


    pub_type: 杂志文章


    authors: Oliveberg M,Fersht AR

    更新日期:1996-02-27 00:00:00

  • Equilibrium and stop-flow kinetic studies of fluorescently labeled DNA substrates with DNA repair proteins XPA and replication protein A.

    abstract::Nucleotide excision repair (NER) is a crucial pathway in the maintenance of genome stability requiring at least two dozen proteins. XPA and RPA have essential roles in the damage recognition step of NER. To better understand the mechanism of their interactions with DNA, we utilized equilibrium and stop-flow kinetic ap...


    pub_type: 杂志文章


    authors: Iakoucheva LM,Walker RK,van Houten B,Ackerman EJ

    更新日期:2002-01-08 00:00:00

  • 1H NMR sequential resonance assignments, secondary structure, and global fold in solution of the major (trans-Pro43) form of bovine calbindin D9k.

    abstract::A wide range of two-dimensional 1H NMR experiments have been used to completely assign the 500-MHz 1H NMR spectrum of recombinant Ca2+-saturated bovine calbindin D9k (76 amino acids, Mr = 8500). In solution, calbindin D9k exists as an equilibrium mixture of isoforms with trans (75%) and cis (25%) isomers of the peptid...


    pub_type: 杂志文章


    authors: Kördel J,Forsén S,Chazin WJ

    更新日期:1989-08-22 00:00:00

  • Comparative studies on the 7-iodo and 7-fluoro derivatives of N-acetoxy-N-2-acetylaminofluorene: binding sites on DNA and conformational change of modified deoxytrinucleotides.

    abstract::GMP and native DNA were reacted with 7-iodo and 7-fluoro derivatives of N-acetoxy-N-2-acetylaminofluorene. It was shown that the 7-halogeno derivatives react on C-8 of guanine. Furthermore the respective amount of arylamidation (covalent linkage on the C-8 of guanine) and arylation (covalent linkage on 2-NH2 groups of...


    pub_type: 杂志文章


    authors: Lefèvre JF,Fuchs RP,Daune MP

    更新日期:1978-06-27 00:00:00

  • Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha-subunit from rat brain.

    abstract::Rat brain and kidney cDNA libraries were constructed and screened with a cDNA insert corresponding to the mRNA for the sheep kidney Na+,K+-ATPase catalytic subunit. The alpha-subunit cDNAs isolated from the kidney library were derived from a single class of messenger RNA, and the brain cDNAs were derived from three cl...


    pub_type: 杂志文章


    authors: Shull GE,Greeb J,Lingrel JB

    更新日期:1986-12-16 00:00:00

  • O-phosphoserine content of intermediate filament subunits.

    abstract::Purified subunits of intermediate filaments obtained from a variety of tissues and cell types contain O-phosphoserine and, in some cases, smaller amounts of O-phosphothreonine. The O-phosphoserine content was estimated by reaction of performic acid oxidized subunits with methylamine in NaOH. Decamin of BHK-21 and CHO ...


    pub_type: 杂志文章


    authors: Steinert PM,Wantz ML,Idler WW

    更新日期:1982-01-05 00:00:00

  • Proton Transfer and Tautomerism in 2-Aminopurine-Thymine and Pyrrolocytosine-Guanine Base Pairs.

    abstract::Pyrrolocytosine (PC) and 2-aminopurine (2AP) are fluorescent nucleobase analogues of the DNA nucleobases cytosine and adenine, respectively, and form base pairs with guanine and thymine. Both fluorescent nucleobases are used extensively as probes for local structure in nucleic acids as the fluorescence properties of P...


    pub_type: 杂志文章


    authors: Bull GD,Thompson KC

    更新日期:2018-07-31 00:00:00

  • DNA degradation by bleomycin: evidence for 2'R-proton abstraction and for C-O bond cleavage accompanying base propenal formation.

    abstract::Reaction of poly(dA-[2'S-3H]dU) with activated bleomycin yields [3H]uracil propenal that completely retains the tritium label. In contrast, we have previously shown that reaction of poly(dA-[2'R-3H]dU) with activated bleomycin affords unlabeled uracil propenal [Wu, J. C., Kozarich, J. W., & Stubbe, J. (1983) J. Biol. ...


    pub_type: 杂志文章


    authors: Ajmera S,Wu JC,Worth L Jr,Rabow LE,Stubbe J,Kozarich JW

    更新日期:1986-10-21 00:00:00

  • Sequence specificity of internal methylation in B77 avian sarcoma virus RNA subunits.

    abstract::Following ribonuclease digestion of methyl-3H-labeled B77 avian sarcoma virus RNA subunits, methylated oligonucleotides were isolated by diethylaminoethylcellulose chromotogrpahy. Partial nucleotide sequences were deduced from the known enzymatic specificities of the ribonucleases. In addition to methylated nucleoside...


    pub_type: 杂志文章


    authors: Dimock K,Stoltzfus CM

    更新日期:1977-02-08 00:00:00

  • Properties of tetrahydropteroylpentaglutamate bound to 10-formyltetrahydrofolate dehydrogenase.

    abstract::A new rapid procedure for purifying 10-formyltetrahydrofolate dehydrogenase results in 90 mg of pure enzyme from two rabbit livers. This abundant liver enzyme is known to bind its product tetrahydropteroylpentaglutamate (H4PteGlu5) so tightly that it does not dissociate during size exclusion chromatography. 10-Formylt...


    pub_type: 杂志文章


    authors: Kim DW,Huang T,Schirch D,Schirch V

    更新日期:1996-12-10 00:00:00

  • Cysteine auxotrophy of human leukemic lymphoblasts is associated with decreased amounts of intracellular cystathionase protein.

    abstract::A series of human lymphoblastoid cell lines derived from nonleukemic donors are known to be cysteine prototrophs (cys+), while several lymphoblastoid lines derived from leukemic donors are cysteine auxotrophs (cys-). We have tested representative cell lines of each type for their content of cystathionase enzyme activi...


    pub_type: 杂志文章


    authors: Glode LM,Kriegler MP,Livingston DM

    更新日期:1981-03-03 00:00:00

  • The putative Na+/H+ antiporter of Vibrio cholerae, Vc-NhaP2, mediates the specific K+/H+ exchange in vivo.

    abstract::The existence of bacterial K(+)/H(+) antiporters that prevent the overaccumulation of potassium in the cytoplasm was predicted by Peter Mitchell almost 50 years ago. The importance of K(+)/H(+) antiport for bacterial physiology is widely recognized, but its molecular mechanisms remain underinvestigated. Here, we demon...


    pub_type: 杂志文章


    authors: Resch CT,Winogrodzki JL,Patterson CT,Lind EJ,Quinn MJ,Dibrov P,Häse CC

    更新日期:2010-03-23 00:00:00

  • pH dependence and structural interpretation of the reactions of Coprinus cinereus peroxidase with hydrogen peroxide, ferulic acid, and 2,2'-azinobis.

    abstract::Steady-state and transient-state analysis of Coprinus cinereus peroxidase, CIP (identical to Arthromyces ramosus peroxidase), was used to characterize the kinetics of the three fundamental steps in heme peroxidase catalysis: compound I (cpd I) formation, cpd I reduction, and compound II (cpd II) reduction. The rate co...


    pub_type: 杂志文章


    authors: Abelskov AK,Smith AT,Rasmussen CB,Dunford HB,Welinder KG

    更新日期:1997-08-05 00:00:00

  • Photochemical reactions of photosystem II in ethylene glycol.

    abstract::The behavior of photosystem II (PSII) reactions was investigated under conditions of decreasing water content by the addition of increasing concentrations of ethylene glycol (EG). The photosynthetic activities were measured for PSII samples either directly in aqueous solutions of EG or in the standard buffer medium fo...


    pub_type: 杂志文章


    authors: Hillier W,Lukins P,Seibert M,Wydrzynski T

    更新日期:1997-01-07 00:00:00

  • A ParE-ParC fusion protein is a functional topoisomerase.

    abstract::Type II topoisomerases are responsible for DNA unlinking during DNA replication and chromosome segregation. Although eukaryotic enzymes are homodimers and prokaryotic enzymes are heterotetramers, both prokaryotic and eukaryotic type II topoisomerases belong to a single protein family. The amino- and carboxyl-terminal ...


    pub_type: 杂志文章


    authors: Lavasani LS,Hiasa H

    更新日期:2001-07-24 00:00:00

  • A novel pentasaccharide sequence GlcA(3-sulfate)(beta1-3)GalNAc(4-sulfate)(beta1-4)(Fuc alpha1-3)GlcA(beta1-3)GalNAc(4-sulfate) in the oligosaccharides isolated from king crab cartilage chondroitin sulfate K and its differential susceptibility to chondroi

    abstract::Novel sulfated tetrasaccharide structures containing 3-O-sulfated GlcA were isolated recently from king crab cartilage chondroitin sulfate K [Sugahara, K., Tanaka, Y., Yamada, S., Seno, N., Kitagawa, H., Haslam, S. M., Morris, H. R., & Dell, A. (1996) J. Biol. Chem. 271, 26745-26754]. In this study, we prepared a seri...


    pub_type: 杂志文章


    authors: Kitagawa H,Tanaka Y,Yamada S,Seno N,Haslam SM,Morris HR,Dell A,Sugahara K

    更新日期:1997-04-01 00:00:00

  • Disulfide-linked bovine beta-lactoglobulin dimers fold slowly, navigating a glassy folding landscape.

    abstract::To gain insight into the folding of large proteins, we constructed a bovine beta-lactoglobulin (beta-lg) dimeric mutant, A34C/C121A beta-lg. In the mutant, a free thiol group of wild-type beta-lg at Cys121 was removed and two beta-lg molecules were linked by a disulfide bridge through Cys34 created at the dimer's inte...


    pub_type: 杂志文章


    authors: Yagi M,Kameda A,Sakurai K,Nishimura C,Goto Y

    更新日期:2008-06-03 00:00:00

  • NMR solution structure of a synthetic troponin C heterodimeric domain.

    abstract::The C-terminal domain from the muscle protein troponin C (TnC) comprises two helix-loop-helix calcium-binding sites (residues 90-162). The assembly of these two sites is governed by calcium binding enabling a synthetic C-terminal domain to be preferentially and stoichiometrically assembled from two synthetic peptides ...


    pub_type: 杂志文章


    authors: Shaw GS,Sykes BD

    更新日期:1996-06-11 00:00:00

  • Structure of the conserved HAMP domain in an intact, membrane-bound chemoreceptor: a disulfide mapping study.

    abstract::The HAMP domain is a conserved motif widely distributed in prokaryotic and lower eukaryotic organisms, where it is often found in transmembrane receptors that regulate two-component signaling pathways. The motif links receptor input and output modules and is essential to receptor structure and signal transduction. Rec...


    pub_type: 杂志文章


    authors: Swain KE,Falke JJ

    更新日期:2007-12-04 00:00:00