Interaction of the fluorescence-labeled secretory component with human polymeric immunoglobulins.

Abstract:

:The secretory component (SC) isolated from human milk was labeled with 2 mol of the fluorescent thiol reagent N-[7-(dimethylamino)-4-methylcoumarinyl]maleimide (DACM) per mol of SC through the reactive disulfide bond of SC. The binding of the labeled SC to polymeric immunoglobulins was examined by gel filtration by measuring the fluorescence of DACM at 478 nm. The labeled SC was bound to immunoglobulin M (IgM) and its (Fc)5 mu fragment and to dimeric immunoglobulin A (IgA). When the labeled SC was bound to IgM or the (Fc)5 mu fragment, the fluorescence of DACM increased about 30%. By use of this fluorescence change, quantitative studies were made on the equilibrium and kinetics of the reversible interactions of the labeled SC with two IgM proteins and their (Fc)5 mu fragments at pH 7.0 and 25 degrees C. All the IgM proteins and their (Fc)5 mu fragments had one binding site per mole of polymers. The affinity constant (6 X 10(8) M-1), the association rate constant (7 X 10(7) M-1 min-1), and the dissociation rate constant (0.1 min-1) of one IgM were different from those of the other IgM (1.7 X 10(9) M-1, 1.0 X 10(8) M-1 min-1, and 0.06 min-1, respectively). However, the values for the (Fc)5 mu fragments of the two proteins were the same (1.9 X 10(9) M-1, 1.1 X 10(8) M-1 min-1, and 0.06 min-1, respectively) and were very similar to those of the IgM with the higher affinity constant.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Biochemistry

journal_title

Biochemistry

authors

Goto Y,Aki K

doi

10.1021/bi00321a070

subject

Has Abstract

pub_date

1984-12-18 00:00:00

pages

6736-44

issue

26

eissn

0006-2960

issn

1520-4995

journal_volume

23

pub_type

杂志文章
  • Platelet-neutrophil-smooth muscle cell interactions: lipoxygenase-derived mono- and dihydroxy acids activate cholesteryl ester hydrolysis by the cyclic AMP dependent protein kinase cascade.

    abstract::Fluid-phase interactions between hematologic cells and those of the vessel wall were studied in order to define a role for lipoxygenase products as cell signals in the control of vascular cholesterol metabolism. A functional parameter for hydroxy acids in this system has not been previously demonstrated. We report her...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00448a030

    authors: Hajjar DP,Marcus AJ,Etingin OR

    更新日期:1989-10-31 00:00:00

  • A kissing complex together with a stable dimer is involved in the HIV-1Lai RNA dimerization process in vitro.

    abstract::Retroviruses contain a dimeric RNA consisting of two identical molecules of genomic RNA. The interaction between the two monomers is thought to occur near their 5'ends. We previously identified a region upstream from the splice donor site, comprising an autocomplementary sequence, responsible for the formation of dime...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi952822s

    authors: Muriaux D,Fossé P,Paoletti J

    更新日期:1996-04-16 00:00:00

  • Purification of botrocetin from Bothrops jararaca venom. Analysis of the botrocetin-mediated interaction between von Willebrand factor and the human platelet membrane glycoprotein Ib-IX complex.

    abstract::Interaction of von Willebrand factor (vWF) with its platelet receptor only occurs in vitro in the presence of a modulator such as ristocetin. We have recently confirmed that the human platelet membrane glycoprotein (GP) Ib-IX complex is the receptor involved in the ristocetin-dependent binding of vWF by reconstitution...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00447a009

    authors: Andrews RK,Booth WJ,Gorman JJ,Castaldi PA,Berndt MC

    更新日期:1989-10-17 00:00:00

  • Identification by proton nuclear magnetic resonance of the histidines in cytochrome b5 modified by diethyl pyrocarbonate.

    abstract::Diethyl pyrocarbonate (DEP) is an electrophilic reagent that is used to modify reversibly the histidine residues of proteins. Unfortunately, the lability of the acylated histidine adduct usually does not permit the isolation and identification of the modified histidine. By use of 500-MHz proton NMR spectroscopy, it ha...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00445a004

    authors: Altman J,Lipka JJ,Kuntz I,Waskell L

    更新日期:1989-09-19 00:00:00

  • Presence of two rhodopsin intermediates responsible for transducin activation.

    abstract::To identify how many rhodopsin intermediates interact with retinal G-protein transducin, the photobleaching process of chicken rhodopsin has been investigated in the presence or absence of transducin by means of time-resolved low-temperature spectroscopy. Singular value decomposition (SVD) analysis of the spectral dat...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi970932o

    authors: Tachibanaki S,Imai H,Mizukami T,Okada T,Imamoto Y,Matsuda T,Fukada Y,Terakita A,Shichida Y

    更新日期:1997-11-18 00:00:00

  • Direct observation of the enzyme-intermediate complex of 5-enolpyruvylshikimate-3-phosphate synthase by 13C NMR spectroscopy.

    abstract::The interaction of 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase, 4,5-dideoxyshikimate 3-phosphate (ddS3P), and [2-13C]-and [3-13C]phosphoenolpyruvate (PEP) has been examined by 13C NMR spectroscopy. Although no resonances due to a dead-end intermediate complex could be detected, an enzyme active site specific fo...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00446a003

    authors: Barlow PN,Appleyard RJ,Wilson JO,Evans JN

    更新日期:1989-10-03 00:00:00

  • Ti(IV) uptake and release by human serum transferrin and recognition of Ti(IV)-transferrin by cancer cells: understanding the mechanism of action of the anticancer drug titanocene dichloride.

    abstract::The organometallic anticancer agent titanocene dichloride, Cp(2)TiCl(2), is now in phase II clinical trials as an anticancer drug, but its mechanism of action is poorly understood. We show here that the interactions of Cp(2)TiCl(2) with human serum transferrin (hTF) and that of Ti(2)-hTF with adenosine triphosphate (A...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi000798z

    authors: Guo M,Sun H,McArdle HJ,Gambling L,Sadler PJ

    更新日期:2000-08-22 00:00:00

  • Photoaffinity labeling of the human receptor for urokinase-type plasminogen activator using a decapeptide antagonist. Evidence for a composite ligand-binding site and a short interdomain separation.

    abstract::Binding of urokinase-type plasminogen activator (uPA) to its cellular receptor (uPAR) renders the cell surface a favored site for plasminogen activation. Recently, a 15-mer peptide antagonist of the uPA-uPAR interaction, with an IC50 value of 10 nM, was identified using phage display technology [Goodson, R. J., Doyle,...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi972787k

    authors: Ploug M,Ostergaard S,Hansen LB,Holm A,Danø K

    更新日期:1998-03-17 00:00:00

  • The metastasis-associated extracellular matrix protein osteopontin forms transient structure in ligand interaction sites.

    abstract::Osteopontin (OPN) is an acidic hydrophilic glycophosphoprotein that was first identified as a major sialoprotein in bones. It functions as a cell attachment protein displaying a RGD cell adhesion sequence and as a cytokine that signals through integrin and CD44 cell adhesion molecules. OPN is also implicated in human ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi200291e

    authors: Platzer G,Schedlbauer A,Chemelli A,Ozdowy P,Coudevylle N,Auer R,Kontaxis G,Hartl M,Miles AJ,Wallace BA,Glatter O,Bister K,Konrat R

    更新日期:2011-07-12 00:00:00

  • Binding of Clinical Inhibitors to a Model Precursor of a Rationally Selected Multidrug Resistant HIV-1 Protease Is Significantly Weaker Than That to the Released Mature Enzyme.

    abstract::We have systematically validated the activity and inhibition of a HIV-1 protease (PR) variant bearing 17 mutations (PR(S17)), selected to represent high resistance by machine learning on genotype-phenotype data. Three of five mutations in PR(S17) correlating with major drug resistance, M46L, G48V, and V82S, and five o...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.6b00012

    authors: Park JH,Sayer JM,Aniana A,Yu X,Weber IT,Harrison RW,Louis JM

    更新日期:2016-04-26 00:00:00

  • Role of the covalent flavin linkage in monomeric sarcosine oxidase.

    abstract::Monomeric sarcosine oxidase (MSOX) is a prototypical member of a recently recognized family of amine-oxidizing enzymes that all contain covalently bound flavin. Mutation of the covalent flavin attachment site in MSOX produces a catalytically inactive apoprotein (apoCys315Ala) that forms an unstable complex with FAD (K...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0607352

    authors: Hassan-Abdallah A,Zhao G,Jorns MS

    更新日期:2006-08-08 00:00:00

  • Circular dichroism and electron microscopy of a core Y61F mutant of the F1 gene 5 single-stranded DNA-binding protein and theoretical analysis of CD spectra of four Tyr --> Phe substitutions.

    abstract::A core Y61F mutant of the gene 5 single-stranded DNA-binding protein (g5p) of f1 bacterial virus aggregated when expressed from a plasmid, but, after refolding in vitro, it behaved much like wild-type and may be a stability or folding mutant. Circular dichroism (CD) titrations showed the same cooperative polynucleotid...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi972545k

    authors: Thompson TM,Mark BL,Gray CW,Terwilliger TC,Sreerama N,Woody RW,Gray DM

    更新日期:1998-05-19 00:00:00

  • Properties of tRNA species modified in the 3'-terminal ribose moiety in an eukaryotic ribosomal system.

    abstract::Phe-tRNAPhe species modified on the 3'-terminal ribose residue were investigated for their ability to participate in individual steps of the elongation cycle using eukaryotic ribosomes from reticulocytes. None of the Phe-tRNAs used, namely Phe-tRNAPhe-C-C-3'dA, Phe-tRNAPhe-C-C-3'-NH2A, and Phe-tRNAPhe-C-C-Aoxi-red, ca...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00661a035

    authors: Baksht E,de Groot N,Sprinzl M,Cramer F

    更新日期:1976-08-10 00:00:00

  • Modulation of mitochondrial complex I activity by reversible Ca2+ and NADH mediated superoxide anion dependent inhibition.

    abstract::Complex I, a key component of the mitochondrial respiratory chain, exhibits diminished activity as a result of cardiac ischemia/reperfusion. Cardiac ischemia/reperfusion is associated with increases in the levels of mitochondrial Ca(2+) and pro-oxidants. In the current in vitro study, we sought evidence for a mechanis...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi049803f

    authors: Sadek HA,Szweda PA,Szweda LI

    更新日期:2004-07-06 00:00:00

  • Disulfide bond assignment in human J chain and its covalent pairing with immunoglobulin M.

    abstract::The assignment of disulfide bonds in human J chain and its covalent pairing with immunoglobulin M was determined under conditions which minimize disulfide bond interchange. We show that in J chain the three intradisulfide bridges are formed between Cys 12 and 100, Cys 71 and 91, and Cys 108 and 133. Previous reports [...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00165a014

    authors: Frutiger S,Hughes GJ,Paquet N,Lüthy R,Jaton JC

    更新日期:1992-12-22 00:00:00

  • Beta-elimination of indole from L-tryptophan catalyzed by bacterial tryptophan synthase: a comparison between reactions catalyzed by tryptophanase and tryptophan synthase.

    abstract::Although tryptophan synthase catalyzes a number of pyridoxal phosphate dependent beta-elimination and beta-replacement reactions that are also catalyzed by tryptophanase, a principal and puzzling difference between the two enzymes lies in the apparent inability of tryptophan synthase to catalyze beta-elimination of in...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00363a010

    authors: Ahmed SA,Martin B,Miles EW

    更新日期:1986-07-29 00:00:00

  • A calcium-specific site influences the structure and activity of the manganese cluster responsible for photosynthetic water oxidation.

    abstract::EPR studies have revealed that removal of calcium using citric acid from the site in spinach photosystem II which is coupled to the photosynthetic O2-evolving process produces a structural change in the manganese cluster responsible for water oxidation. If done in the dark, this yields a modified S1' oxidation state w...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00450a032

    authors: Sivaraja M,Tso J,Dismukes GC

    更新日期:1989-11-28 00:00:00

  • Vibrationally enhanced hydrogen tunneling in the Escherichia coli thymidylate synthase catalyzed reaction.

    abstract::The enzyme thymidylate synthase (TS) catalyzes a complex reaction that involves forming and breaking at least six covalent bonds. The physical nature of the hydride transfer step in this complex reaction cascade has been studied by means of isotope effects and their temperature dependence. Competitive kinetic isotope ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi036124g

    authors: Agrawal N,Hong B,Mihai C,Kohen A

    更新日期:2004-02-24 00:00:00

  • Phosphorylation mechanism of nucleoside diphosphate kinase: 31P-nuclear magnetic resonance studies.

    abstract::The phosphorylation mechanism of Dictyostelium discoideum nucleoside diphosphate (NDP) kinase was investigated by NMR. 31P chemical shifts were measured on both native and denatured enzyme. In the enzymatically phosphorylated enzyme denatured by 9 M urea or 7 M guanidine hydrochloride, the NDP kinase phosphohistidine ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00038a043

    authors: Lecroisey A,Lascu I,Bominaar A,Véron M,Delepierre M

    更新日期:1995-09-26 00:00:00

  • Major groove (R)-alpha-(N6-adenyl)styrene oxide adducts in an oligodeoxynucleotide containing the human N-ras codon 61 sequence: conformations of the R(61,2) and R(61,3) sequence isomers from 1H NMR.

    abstract::Conformations of (R)-alpha-(N6-adenyl)styrene oxide adducts at positions X6 in d(CGGACXAGAAG).d(CTTCTTGTCCG) and X7 in d(CGGACAXGAAG).d(CTTCTTGTCCG), incorporating codons 60, 61 (underlined), and 62 of the human n-ras protooncogene, were refined from 1H NMR data. These were the R(61,2) and R(61,3) adducts. Chemical sh...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00043a008

    authors: Feng B,Zhou L,Passarelli M,Harris CM,Harris TM,Stone MP

    更新日期:1995-10-31 00:00:00

  • High resolution proton nuclear magnetic resonance investigation of the structural and dynamic properties of d(C15A15)-d(T15G15).

    abstract::The high resolution proton nuclear magnetic resonance (NMR) spectra of the synthetic DNA block polymer d(C15A15)-d(T15G15) were studied in order to more completely understand telestability in DNA, and to provide fundamental NMR data on DNA helices and random coils. Spectra were measured in the spectral region from 0 t...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00622a031

    authors: Early TA,Kearns DR,Burd JF,Larson JE,Wells RD

    更新日期:1977-02-08 00:00:00

  • In vitro interaction between ceruloplasmin and human serum transferrin.

    abstract::The thermodynamics of the interactions of serum apotransferrin (T) and holotransferrin (TFe(2)) with ceruloplasmin (Cp), as well as those of human lactoferrin (Lf), were assessed by fluorescence emission spectroscopy. Cp interacts with two Lf molecules. The first interaction depends on pH and μ, whereas the second doe...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi1014503

    authors: Ha-Duong NT,Eid C,Hémadi M,El Hage Chahine JM

    更新日期:2010-12-07 00:00:00

  • Role of enzyme-peptide substrate backbone hydrogen bonding in determining protein kinase substrate specificities.

    abstract::As part of a search for peptides that have specificity for selected protein kinases, the possibility that adenosine cyclic 3',5'-phosphate dependent protein kinase (A-kinase) recognizes the hydrogen-bonding potential of its peptide substrates was investigated. A-Kinase catalyzes the phosphorylation of five N alpha-met...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00388a041

    authors: Thomas NE,Bramson HN,Miller WT,Kaiser ET

    更新日期:1987-07-14 00:00:00

  • Phosphorylation of beta III-tubulin.

    abstract::There is considerable evidence that mammalian beta-tubulin is phosphorylated. Specifically, of the seven beta isotypes, the phosphorylated one is beta III, the isotype found almost entirely in neurons. The phosphate is added at a serine and perhaps a tyrosine near the C-terminus. All the evidence to date has been gath...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi951247p

    authors: Khan IA,Ludueña RF

    更新日期:1996-03-26 00:00:00

  • Relationship between Amphipathic β Structures in the β1 Domain of Apolipoprotein B and the Properties of the Secreted Lipoprotein Particles in McA-RH7777 Cells.

    abstract::Our previous studies demonstrated that the first 1000 amino acid residues (the βα1 domain) of human apolipoprotein (apo) B-100, termed apoB:1000, are required for the initiation of lipoprotein assembly and the formation of a monodisperse stable phospholipid (PL)-rich particle. The objectives of this study were (a) to ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.6b01174

    authors: Manchekar M,Kapil R,Sun Z,Segrest JP,Dashti N

    更新日期:2017-08-08 00:00:00

  • Potentiation of progesterone receptor-mediated transcription by the immunosuppressant FK506.

    abstract::The nontransformed steroid receptors contain several non-steroid binding proteins, such as hsp90, hsp70, and p59. Recently, we and others have shown that p59 (FKBP59) is an immunophilin which binds two potent immunosuppressants, FK506 and rapamycin. This raises the possibility that FK506 or rapamycin may modify the fu...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00201a014

    authors: Tai PK,Albers MW,McDonnell DP,Chang H,Schreiber SL,Faber LE

    更新日期:1994-09-06 00:00:00

  • Identification of proximal and distal axial ligands in Leishmania major pseudoperoxidase.

    abstract::Previous optical and electron paramagnetic resonance (EPR) spectroscopic studies of the newly discovered peroxynitrite scavenging pseudoperoxidase from Leishmania major (LmPP) suggested that ferric LmPP contained a six-coordinate low-spin (6cLS) heme with a thiolate ligand, presumably a cysteine, bound to its heme iro...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi401343t

    authors: Saha R,Bose M,Sen Santara S,Roy J,Adak S

    更新日期:2013-12-10 00:00:00

  • Binding efficiency of protein-protein complexes.

    abstract::We examine the relationship between binding affinity and interface size for reversible protein-protein interactions (PPIs), using cytokines from the tumor necrosis factor (TNF) superfamily and their receptors as a test case. Using surface plasmon resonance, we measured single-site binding affinities for binding of the...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi301039t

    authors: Day ES,Cote SM,Whitty A

    更新日期:2012-11-13 00:00:00

  • Effect of phosphorylation on hydrogen-bonding interactions of the active site histidine of the phosphocarrier protein HPr of the phosphoenolpyruvate-dependent phosphotransferase system determined by 15N NMR spectroscopy.

    abstract::The phosphocarrier protein HPr of the phosphoenolpyruvate-dependent sugar transport system of Escherichia coli can exist in a phosphorylated and a nonphosphorylated form. During phosphorylation, the phosphoryl group is carried on a histidine residue, His15. The hydrogen-bonding state of this histidine was examined wit...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00487a026

    authors: van Dijk AA,de Lange LC,Bachovchin WW,Robillard GT

    更新日期:1990-09-04 00:00:00

  • Thermal behavior of synthetic sphingomyelin-cholesterol dispersions.

    abstract::The thermotropic behavior of aqueous dispersions of palmitoylsphingomyelin-cholesterol and lignoceryl-sphingomyelin-cholesterol mixtures has been examined by high-sensitivity differential scanning calorimetry. When less than 25 mol % cholesterol is mixed with either sphingomyelin, the calorimetric endotherm is compose...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00577a042

    authors: Estep TN,Mountcastle DB,Barenholz Y,Biltonen RL,Thompson TE

    更新日期:1979-05-15 00:00:00