Proton transfer limits protein translocation rate by the thylakoid DeltapH/Tat machinery.

abstract：:The effects of structural modifications of the flavin mononucleotide (FMN) prosthetic group of Clostridium pasteurianum flavodoxin on the kinetics of electron transfer to the oxidized form (from 5-deazariboflavin semiquinone produced by laser flash photolysis) and from the semiquinone form (to horse heart cytochrome c...

journal_title：Biochemistry

authors： Simondsen RP,Tollin G

更新日期：1983-06-07 00:00:00

abstract：:Nitric oxide is produced in mammals by the nitric oxide synthase (NOS) isoforms at a catalytic site comprising a heme associated with a biopterin cofactor. Through genome sequencing, proteins that are highly homologous to the oxygenase domain of NOSs have been identified, in particular in bacteria. The active site is ...

journal_title：Biochemistry

authors： Weisslocker-Schaetzel M,Lembrouk M,Santolini J,Dorlet P

更新日期：2017-02-07 00:00:00

abstract：:Most A/T specific heterocyclic diamidine derivatives need at least four A/T base pairs for tight binding to the DNA minor groove. Addition of a GC base pair to A/T sequences typically causes a large decrease in binding constant. The ability to target biologically important sequences of DNA could be significantly incre...

journal_title：Biochemistry

authors： Rahimian M,Kumar A,Say M,Bakunov SA,Boykin DW,Tidwell RR,Wilson WD

更新日期：2009-02-24 00:00:00

abstract：:Both the male and the female of Mastomys natalensis, an African rat, have high levels of nerve growth factor (NGF) in their submaxillary glands. Mastomys high molecular weight (HMW) NGF was purified by gel filtration and ion-exchange chromatography and was compared with HMW NGF from the male mouse submaxillary gland. ...

journal_title：Biochemistry

authors： Darling TL,Fahnestock M

更新日期：1988-09-06 00:00:00

abstract：:Among the molecular strategies bacteria have set up to quickly match their transcriptional program to new environments, changes in sequence-mediated DNA curvature play a crucial role. Bacterial promoters, especially those of mesophilic bacteria, are in general preceded by a curved region. The marked thermosensitivity ...

journal_title：Biochemistry

authors： Prosseda G,Mazzola A,Di Martino ML,Tielker D,Micheli G,Colonna B

更新日期：2010-04-06 00:00:00

abstract：:Most terpene synthase reactions follow Markovnikov rules for formation of high-energy carbenium ion intermediates. However, there are notable exceptions. For example, pentalenene synthase (PS) undergoes an initial anti-Markovnikov cyclization reaction followed by a 1,2-hydride shift to form an intermediate humulyl cat...

journal_title：Biochemistry

authors： Matos JO,Kumar RP,Ma AC,Patterson M,Krauss IJ,Oprian DD

更新日期：2020-09-08 00:00:00

abstract：:A method is described for measuring equilibrium constants of DNA-protein interactions using gel chromatography. This technique has been used to study the sequence-specific interaction of the HinfI restriction endonuclease with DNA. HinfI has a monomeric molecular weight of 31000 and exists as a dimer in its active for...

journal_title：Biochemistry

authors： Frankel AD,Ackers GK,Smith HO

更新日期：1985-06-04 00:00:00

abstract：:We have developed a new genomic sequencing method for detecting, with resolution at the nucleotide level, the interstrand DNA cross-links induced by 4,5',8-trimethylpsoralen along single-copy genes in mammalian cells. The cross-links (diadducts) initially formed are converted into monoadducts by alkali reversal prior ...

journal_title：Biochemistry

authors： Komura J,Ikehata H,Hosoi Y,Riggs AD,Ono T

更新日期：2001-04-03 00:00:00

abstract：:Natriuretic peptide receptor A (NPRA) is a noncovalent homodimeric receptor, composed of an extracellular domain (ECD) with a ligand-binding site, a single transmembrane domain (TM), and an intracellular domain (ICD) exhibiting guanylyl cyclase activity. NPRA activation by atrial natriuretic peptide (ANP) leads to cGM...

journal_title：Biochemistry

authors： Parat M,Blanchet J,De Léan A

更新日期：2010-06-08 00:00:00

abstract：:Human carbonic anhydrase II (CAII) possesses a binding site for an acidic motif (D887ADD) within the carboxyl-terminal region (Ct) of the human erythrocyte chloride/bicarbonate anion exchanger, AE1. In this study, the amino acid sequence comprising this AE1 binding site was localized to the first 17 residues of CAII, ...

journal_title：Biochemistry

authors： Vince JW,Carlsson U,Reithmeier RA

更新日期：2000-11-07 00:00:00

abstract：:The phagocyte superoxide-generating NADPH oxidase, a multicomponent, membrane-bound electron transport chain, consists of cytochrome b558, p47-phox, p67-phox, and p21rac1 or p21rac2. The mechanisms of oxidase assembly are poorly understood. In previous studies using a cell-free NADPH oxidase system, we showed that pre...

journal_title：Biochemistry

authors： Kleinberg ME,Malech HL,Mital DA,Leto TL

更新日期：1994-03-08 00:00:00

abstract：:We have studied the effect of melittin, a basic membrane-binding peptide, on Ca-ATPase activity and on protein and lipid dynamics in skeletal sarcoplasmic reticulum (SR), using time-resolved phosphorescence and fluorescence spectroscopy. Melittin completely inhibits Ca-ATPase activity, with half-maximal inhibition at ...

journal_title：Biochemistry

authors： Voss J,Birmachu W,Hussey DM,Thomas DD

更新日期：1991-07-30 00:00:00

abstract：:The apolipoprotein apoE plays a key role in cholesterol and lipid metabolism. There are three isoforms of this protein, one of which, apoE4, is the major risk factor for Alzheimer's disease. At micromolar concentrations all lipid-free apoE isoforms exist primarily as monomers, dimers, and tetramers. However, the molec...

journal_title：Biochemistry

authors： Garai K,Baban B,Frieden C

更新日期：2011-04-05 00:00:00

abstract：:We have examined the ability of the Escherichia coli single-stranded DNA binding protein (SSB) tetramer to form its different binding modes on poly(dC), poly(U), and poly(A) over a range of NaCl and NaF concentrations for comparison with previous studies with poly(dT). In reverse titrations with poly(U) and poly(A) at...

journal_title：Biochemistry

authors： Lohman TM,Bujalowski W

更新日期：1994-05-24 00:00:00

abstract：:The carbohydrate binding specificity of the seed lectin from Artocarpus integrifolia, artocarpin, has been elucidated by the enzyme-linked lectin absorbent assay [Misquith, S., et al (1994) J. Biol. Chem. 269, 30393-30401], wherein it was demonstrated to be a Man/Glc specific lectin with high affinity for the trisacch...

journal_title：Biochemistry

authors： Rani PG,Bachhawat K,Reddy GB,Oscarson S,Surolia A

更新日期：2000-09-05 00:00:00

abstract：:The pigment composition of the isolated photosystem II reaction center complex in its most stable and pure form currently is a matter of considerable debate. In this contribution, we present a new method based on a combination of gel filtration chromatography and diode array detection to analyze the composition of pho...

journal_title：Biochemistry

authors： Eijckelhoff C,van Roon H,Groot ML,van Grondelle R,Dekker JP

更新日期：1996-10-01 00:00:00

abstract：:Dynamin is a large GTPase involved in the regulation of membrane constriction and fission during receptor-mediated endocytosis. Dynamin contains a pleckstrin-homology domain which is essential for endocytosis and which binds to anionic phospholipids. Here, we show for the first time that dynamin is a membrane-active m...

journal_title：Biochemistry

authors： Burger KN,Demel RA,Schmid SL,de Kruijff B

更新日期：2000-10-10 00:00:00

abstract：:L6 is a monoclonal antitumor antibody which recognizes an epitope located in a 42-residue extracellular domain of a tumor-associated approximately 22 kDa glycoprotein antigen. The L6 mAb localizes to solid tumors in vivo and triggers complement activation and antibody-dependent cellular cytotoxicity. It has been the s...

journal_title：Biochemistry

authors： Stenzel-Johnson PR,Yelton D,Bajorath J

更新日期：1994-12-06 00:00:00

abstract：:The contributions to thermostability of interactions within the beta-sheet region of rubredoxins (Rds) were investigated by examining proteins in which beta-strand sequences of Rds from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) and the mesophilic bacterium Clostridium pasteurianum (Cp) were interchanged....

journal_title：Biochemistry

authors： Eidsness MK,Richie KA,Burden AE,Kurtz DM Jr,Scott RA

更新日期：1997-08-26 00:00:00

abstract：:Cytomegaloviruses (CMVs) are ubiquitous species-specific viruses that establish acute, persistent, and latent infections. Both human and mouse CMVs encode proteins that inhibit the activation of natural killer (NK) cells by downregulating cellular ligands for the NK cell activating receptor, NKG2D. The MCMV glycoprote...

journal_title：Biochemistry

authors： Zhi L,Mans J,Paskow MJ,Brown PH,Schuck P,Jonjić S,Natarajan K,Margulies DH

更新日期：2010-03-23 00:00:00

abstract：:The interaction of Ca2+ and vanadate with fluorescein isothiocyanate (FITC) labeled sarcoplasmic reticulum (SR) Ca2+-ATPase has been studied by following the kinetics of changes in the reporter group fluorescence and equilibrium fluorescence levels. The vanadate species bound to the enzyme is clearly monomeric orthova...

journal_title：Biochemistry

authors： Markus S,Priel Z,Chipman DM

更新日期：1989-01-24 00:00:00

abstract：:The fluorescence properties of three variants of alpha-lactalbumin (alpha-LA) containing a single tryptophan residue were investigated under native, molten globule, and unfolded conditions. These proteins have levels of secondary structure and stability similar to those of the wild type. The fluorescence signal in the...

journal_title：Biochemistry

authors： Chakraborty S,Ittah V,Bai P,Luo L,Haas E,Peng Z

更新日期：2001-06-19 00:00:00

abstract：:Endonuclease VIII (Nei) is one of three enzymes in Escherichia coli that are involved in base-excision repair of oxidative damage to DNA. We investigated the substrate specificity and excision kinetics of this DNA glycosylase for bases in DNA that have been damaged by free radicals. Two different DNA substrates were p...

journal_title：Biochemistry

authors： Dizdaroglu M,Burgess SM,Jaruga P,Hazra TK,Rodriguez H,Lloyd RS

更新日期：2001-10-09 00:00:00

abstract：:Urea-induced unfolding of Na,K-ATPase from pig kidney and from shark salt gland was studied by electron paramagnetic resonance (EPR) spectroscopy of a nitroxyl derivative of maleimide covalently attached to sulfhydryl groups which are essential for activity. Urea-induced structural changes lead to the inhibition of Na...

journal_title：Biochemistry

authors： Babavali M,Esmann M,Fedosova NU,Marsh D

更新日期：2009-09-29 00:00:00

abstract：:Formylglycinamide ribonucleotide amidotransferase (FGAR-AT) catalyzes the ATP- and glutamine-dependent formation of formylglycinamidine ribonucleotide, ADP, P(i), and glutamate in the fourth step of de novo purine biosynthesis. Like all amidotransferases (ATs), FGAR-AT is proposed to channel ammonia between a glutamin...

journal_title：Biochemistry

authors： Hoskins AA,Anand R,Ealick SE,Stubbe J

更新日期：2004-08-17 00:00:00

abstract：:Circular dichroism (CD) spectra at variable temperatures have been recorded for human cytosolic sialidase NEU2 in buffered water solutions and in the presence of divalent cations. The results show the prevalence of β-strands together with a considerable amount of α-helical structure, while in the solid state, from bot...

journal_title：Biochemistry

authors： Monti E,Benaglia G,Mozzi A,Fusi P,Longhi G,Gangemi F,Castiglioni E,Woody RW,Fornili SL,Abbate S

更新日期：2014-08-19 00:00:00

abstract：:High-resolution proton nuclear magnetic resonance (1H NMR) measurements were made on myosin, heavy meromyosin (HMM), myosin subfragment 1 (S1), light meromyosin (LMM), and actin. A strong signal from amino acid side chains undergoing motions too fast to be accounted for by simple rotations of groups on a rigid backbon...

journal_title：Biochemistry

authors： Highsmith S,Akasaka K,Konrad M,Goody R,Holmes K,Wade-Jardetzky N,Jardetzky O

更新日期：1979-09-18 00:00:00

abstract：:Perturbations within the transactivation and carboxy-terminal domains of HIV-1 Rev were examined for effects on Rev responsive element (RRE) binding activities in vitro and biological activity in vivo. Binding affinities, specificities, and multimerization of the transactivation mutants M10 and Rev/Rex M10-16 on the R...

journal_title：Biochemistry

authors： Daly TJ,Rennert P,Lynch P,Barry JK,Dundas M,Rusche JR,Doten RC,Auer M,Farrington GK

更新日期：1993-08-31 00:00:00

abstract：:The disulfide bond location of a homodimeric peptide, prothoracicotropic hormone (PTTH) of the silkworm, Bombyx mori, was determined by a combination of partial reduction and sequence analysis of peptide fragments generated through a partial reduction of PTTH followed by alkylation and enzyme digestion. The partial re...

journal_title：Biochemistry

authors： Ishibashi J,Kataoka H,Isogai A,Kawakami A,Saegusa H,Yagi Y,Mizoguchi A,Ishizaki H,Suzuki A

更新日期：1994-05-17 00:00:00

abstract：:The DNA interstrand cross-link (ICL) resulting from the C4'-oxidized abasic site (C4-AP) is a unique clustered lesion comprised of a cross-link adjacent to a nick. The ICL is a substrate for the UvrABC nucleotide excision repair system. The strand containing the nick is preferentially incised, but the nick influences ...

journal_title：Biochemistry

authors： Sczepanski JT,Jacobs AC,Van Houten B,Greenberg MM

更新日期：2009-08-18 00:00:00