Dynamin is membrane-active: lipid insertion is induced by phosphoinositides and phosphatidic acid.

abstract：:Although the importance of interactions involving both the cytosolic and transmembrane regions of SecY and SecE has been documented, no information has been available for the physical contact sites of these translocase subunits in their cytosolic domains. We now carried out site-specific cross-linking experiments to i...

journal_title：Biochemistry

authors： Satoh Y,Mori H,Ito K

更新日期：2003-06-24 00:00:00

abstract：:The physiological effects of anesthetics have been ascribed to their interaction with hydrophobic sites within functionally relevant CNS proteins. Studies have shown that volatile anesthetics compete for luciferin binding to the hydrophobic substrate binding site within firefly luciferase and inhibit its activity (Fra...

journal_title：Biochemistry

authors： Ho C,Shanmugasundararaj S,Miller KW,Malinowski SA,Cook AC,Slater SJ

更新日期：2008-09-09 00:00:00

abstract：:Avian magnetoreception is assumed to occur in the retina. Although its molecular mechanism is unclear, magnetic field-dependent formation and the stability of radical-containing photointermediate(s) are suggested to play key roles in a hypothesis called the radical pair mechanism. Chicken cryptochrome4 (cCRY4) has bee...

journal_title：Biochemistry

authors： Otsuka H,Mitsui H,Miura K,Okano K,Imamoto Y,Okano T

更新日期：2020-09-29 00:00:00

abstract：:The effects of phosphorylation at Ser40 of rat tyrosine hydroxylase on the affinities of catechols have been determined with both the ferric and ferrous forms of the enzyme. Phosphorylation had no effect on the Ki value for the inhibition of the ferrous enzyme by either dopamine or DOPA when the initial rate of turnov...

journal_title：Biochemistry

authors： Ramsey AJ,Fitzpatrick PF

更新日期：1998-06-23 00:00:00

abstract：:Cysteinyl S-nitrosylation has emerged as an important post-translational modification affecting protein function in health and disease. Great emphasis has been placed on global, unbiased quantification of S-nitrosylated proteins because of physiologic and oxidative stimuli. However, current strategies have been hamper...

journal_title：Biochemistry

authors： Wiktorowicz JE,Stafford S,Rea H,Urvil P,Soman K,Kurosky A,Perez-Polo JR,Savidge TC

更新日期：2011-06-28 00:00:00

abstract：:The C-terminal domain (CT) of apolipoprotein E (apoE), a critical protein involved in cholesterol transport in the plasma and brain, plays an important role in high-affinity lipoprotein binding. Although high-resolution structural information is available for the N-terminal domain of apoE, the structural organization ...

journal_title：Biochemistry

authors： Patel AB,Khumsupan P,Narayanaswami V

更新日期：2010-03-02 00:00:00

abstract：:Protein C is a vitamin K dependent protein present in bovine plasma (Stenflo, J. (1976), J. Biol. Chem. 251, 355). It is a glycoprotein (mol wt approximately 62 000) composed of a heavy chain (mol wt 41 000) and a light chain (mol wt 21 000). The heavy chain has an amino-terminal sequence of Asp-Thr-Asn-Gln and contai...

journal_title：Biochemistry

authors： Kisiel W,Ericsson LH,Davie EW

更新日期：1976-11-02 00:00:00

abstract：:RP2 is a ubiquitously expressed protein encoded by a gene associated with X-linked retinitis pigmentosa (XLRP), a retinal degenerative disease that causes severe vision loss. Previous in vitro studies have shown that RP2 binds to ADP ribosylation factor-like 3 (Arl3) and activates its intrinsic GTPase activity, but th...

journal_title：Biochemistry

authors： Holopainen JM,Cheng CL,Molday LL,Johal G,Coleman J,Dyka F,Hii T,Ahn J,Molday RS

更新日期：2010-09-07 00:00:00

abstract：:Binding studies of the interaction of immobilized 1alpha- and 17alpha-aminoalkyl derivatives of 5alpha-dihydrotestosterone (DHT) with purified N-deglycosylated homodimeric human sex hormone-binding globulin (SHBG) were performed using a surface plasmon resonance biosensor. These 1alpha- and 17alpha-derivatives with sp...

journal_title：Biochemistry

authors： Metzger J,Schnitzbauer A,Meyer M,Söder M,Cuilleron CY,Hauptmann H,Huber E,Luppa PB

更新日期：2003-11-25 00:00:00

abstract：:The structural and functional roles of two conserved active site residues, Arg-97 and Arg-132, in chicken muscle adenylate kinase (AK) were evaluated by site-directed mutagenesis in conjunction with one- and two-dimensional proton nuclear magnetic resonance (NMR), kinetics, and guanidine hydrochloride-induced denatura...

journal_title：Biochemistry

authors： Dahnke T,Shi Z,Yan H,Jiang RT,Tsai MD

更新日期：1992-07-14 00:00:00

abstract：:Proton and phosphorus-31 nuclear magnetic resonance (1H and 31P NMR) studies of the interaction between a tridecapeptide pheromone, the alpha-factor of Saccharomyces cerevisiae, and sonicated lipid vesicles are reported. 31P NMR studies demonstrate that there is interaction of the peptide with the phosphorus headgroup...

journal_title：Biochemistry

authors： Jelicks LA,Broido MS,Becker JM,Naider FR

更新日期：1989-05-16 00:00:00

abstract：:Fragile X syndrome, the most common cause of inherited mental retardation, is caused by the absence of the fragile X mental retardation protein (FMRP). The emerging picture is that FMRP is involved in repression of translation through a complex network of protein-protein and protein-RNA interactions. Very little struc...

journal_title：Biochemistry

authors： Adinolfi S,Ramos A,Martin SR,Dal Piaz F,Pucci P,Bardoni B,Mandel JL,Pastore A

更新日期：2003-09-09 00:00:00

abstract：:Doublet signal and singlet-like signal induced in Ca(2+)-depleted PS II were investigated by pulsed EPR in one-dimensionally oriented photosystem (PS) II membranes. The doublet signal showed marked angular dependent change in its spectrum in term of the applied magnetic field, indicating that the magnetic dipole-dipol...

journal_title：Biochemistry

authors： Mino H,Kawamori A,Ono T

更新日期：2000-09-12 00:00:00

abstract：:Following a 1-h [3H]uridine pulse of cells of a human colon carcinoma line, 15% of the radioactivity in heterogeneous nuclear RNA associated with both chromatin and nuclear ribonucleoprotein was not digested to acid soluble fragments during a 2-h incubation with staphylococcal nuclease (EC 3.1.4.7). These [3H]uridine-...

journal_title：Biochemistry

authors： Augenlicht LH,McCormick M,Lipkin M

更新日期：1976-08-24 00:00:00

abstract：:Little is known about the extent to which protein flexibility contributes to antigen-antibody recognition and cross-reactivity. Using short coil peptides (leucine zippers) as model antigens, we demonstrate that a monoclonal antibody can force a noncognate peptide into a conformation that is similar to the conformation...

journal_title：Biochemistry

authors： Leder L,Berger C,Bornhauser S,Wendt H,Ackermann F,Jelesarov I,Bosshard HR

更新日期：1995-12-19 00:00:00

abstract：:Interaction of von Willebrand factor (vWF) with its platelet receptor only occurs in vitro in the presence of a modulator such as ristocetin. We have recently confirmed that the human platelet membrane glycoprotein (GP) Ib-IX complex is the receptor involved in the ristocetin-dependent binding of vWF by reconstitution...

journal_title：Biochemistry

authors： Andrews RK,Booth WJ,Gorman JJ,Castaldi PA,Berndt MC

更新日期：1989-10-17 00:00:00

abstract：:Thermodynamic parameters of helix formation were measured spectroscopically for 16 oligoribonucleotides containing either internal GU mismatches or the corresponding AU pairs. Internal GU mismatches stabilize each helix, but not as much as the corresponding AU pairs. The differences in the enthalpy and entropy changes...

journal_title：Biochemistry

authors： Sugimoto N,Kierzek R,Freier SM,Turner DH

更新日期：1986-09-23 00:00:00

abstract：:Catalysis by glycogen phosphorylase involves a mechanism in which binding of one substrate tightens the binding of the other substrate to produce a productive ternary enzyme-substrate complex. In this work the molecular basis for this synergism is probed in crystallographic studies on ternary complexes in which the gl...

journal_title：Biochemistry

authors： Mitchell EP,Withers SG,Ermert P,Vasella AT,Garman EF,Oikonomakos NG,Johnson LN

更新日期：1996-06-11 00:00:00

abstract：:The potency of cholesterol to affect the acyl chain order and dynamics of cardiolipin membranes in the liquid-crystalline state was monitored by steady-state and time-resolved fluorescence anisotropy as well as excited-state lifetime measurements with cis- and trans-parinaric acids as probes. Up to a cholesterol mole ...

journal_title：Biochemistry

authors： Gallay J,Vincent M

更新日期：1986-05-06 00:00:00

abstract：:Chicken histone H4, labeled separately at Met-84 with N-[[(iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonic acid and 5-(iodoacetamido)fluorescein, was reassociated with unlabeled histones H2A, H2B, and H3 and 146 base pairs of DNA to produce fluorescently labeled nucleosomes having physical characteristics virtually...

journal_title：Biochemistry

authors： Chung DG,Lewis PN

更新日期：1986-09-09 00:00:00

abstract：:Nucleolin, also called protein C23, is a RNA-associated protein implicated in the early stages of ribosome assembly. To study the general conformation and map the nucleic acid binding regions, rat nucleolin was subjected to limited proteolysis using trypsin and chymotrypsin in the presence or absence of poly(G). The c...

journal_title：Biochemistry

authors： Olson MO,Kirstein MN,Wallace MO

更新日期：1990-06-19 00:00:00

abstract：:The Rieske protein from Thermus thermophilus (TtRp) and a truncated version of the protein (truncTtRp), produced to achieve a low-pH crystallization condition, have been characterized using UV-visible and circular dichroism spectroscopies. TtRp and truncTtRp undergo a change in the UV-visible spectra with increasing p...

journal_title：Biochemistry

authors： Konkle ME,Muellner SK,Schwander AL,Dicus MM,Pokhrel R,Britt RD,Taylor AB,Hunsicker-Wang LM

更新日期：2009-10-20 00:00:00

abstract：:Ribonuclease T1 was crystallized in the presence of vanadate(V). The crystal structure was solved by molecular replacement and refined by least-squares methods using stereochemical restraints. The refinement was based on data between 10 and 1.8 A and converged at a crystallographic R factor of 0.137. Except for the su...

journal_title：Biochemistry

authors： Kostrewa D,Choe HW,Heinemann U,Saenger W

更新日期：1989-09-19 00:00:00

abstract：:A high molecular weight inert molecule, poly(ethylene glycol) (PEG), or a soluble protein, ovalbumin, causes parallel bundles of actin filaments in a crystalline-like structure under physiological conditions of ionic compositions and pH. The bundle formation depends on the molecular weight of PEG, and a larger molecul...

journal_title：Biochemistry

authors： Suzuki A,Yamazaki M,Ito T

更新日期：1989-07-25 00:00:00

abstract：:We have studied the binding nature of an aromatic aldehyde to the catalytic site of liver alcohol dehydrogenase from horse (LADH) using preresonance Raman spectroscopy. The compound p-(dimethylamino)benzaldehyde (DABA) is converted to the corresponding alcohol in the presence of nicotinamide adenine dinucleotide (NADH...

journal_title：Biochemistry

authors： Callender R,Chen D,Lugtenburg J,Martin C,Rhee KW,Sloan D,Vandersteen R,Yue KT

更新日期：1988-05-17 00:00:00

abstract：:From the larger set of 191 variants at all the variable contact positions in the turkey ovomucoid third domain, we selected a subset that consists of Asp, Glu, His, and Lys residues at eight of the nine contiguous P6-P3' positions (residues 13-21), the exception being P3-Cys16 which is involved in a conserved disulfid...

journal_title：Biochemistry

authors： Song J,Laskowski M Jr,Qasim MA,Markley JL

更新日期：2003-03-18 00:00:00

abstract：:Serine hydroxymethyltransferase (SHMT) catalyzes the reversible cleavage of serine to form glycine and single carbon groups that are essential for many biosynthetic pathways. SHMT requires both pyridoxal phosphate (PLP) and tetrahydropteroylpolyglutamate (H4PteGlun) as cofactors, the latter as a carrier of the single ...

journal_title：Biochemistry

authors： Scarsdale JN,Kazanina G,Radaev S,Schirch V,Wright HT

更新日期：1999-06-29 00:00:00

abstract：:The sequences and three-dimensional structures of the galactokinase, homoserine kinase, mevalonate kinase, and phosphomevalonate kinase (GHMP) family were compared to identify highly conserved surface residues. The functions of these solvent-accessible residues were assessed by determining the effects of their substit...

journal_title：Biochemistry

authors： Andreassi JL 2nd,Leyh TS

更新日期：2004-11-23 00:00:00

abstract：:In an effort to extend automated Edman degradation to nanomole quantities of protein, the method of sequenator analysis described by Edman and Begg (Edman, P., and Begg, G. (1967), Eur. J. Biochem. 1, 80) was modified to permit long degradations in the absence of carrier proteins. By using an aqueous 0.1 M Quadrol pro...

journal_title：Biochemistry

authors： Brauer AW,Margolies MN,Haber E

更新日期：1975-07-01 00:00:00

abstract：:To study whether protein kinase C (PKC) isoforms can interact with protein-tyrosine-phosphatases (PTPs) which are connected to the insulin signaling pathway, we co-overexpressed PKC isoforms together with insulin receptor, docking proteins, and the PTPs SHP1 and SHP2 in human embryonic kidney (HEK) 293 cells. After ph...

journal_title：Biochemistry

authors： Strack V,Krützfeldt J,Kellerer M,Ullrich A,Lammers R,Häring HU

更新日期：2002-01-15 00:00:00