当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Interaction and localization of the retinitis pigmentosa protein RP2 and NSF in retinal photoreceptor cells.

Interaction and localization of the retinitis pigmentosa protein RP2 and NSF in retinal photoreceptor cells.

Abstract：

:RP2 is a ubiquitously expressed protein encoded by a gene associated with X-linked retinitis pigmentosa (XLRP), a retinal degenerative disease that causes severe vision loss. Previous in vitro studies have shown that RP2 binds to ADP ribosylation factor-like 3 (Arl3) and activates its intrinsic GTPase activity, but the function of RP2 in the retina, and in particular photoreceptor cells, remains unclear. To begin to define the role of RP2 in the retina and XLRP, we have conducted biochemical studies to identify proteins in retinal cell extracts that interact with RP2. Here, we show that RP2 interacts with N-ethylmaleimide sensitive factor (NSF) in retinal cells as well as cultured embryonic kidney (HEK293) cells by mass spectrometry-based proteomics and biochemical analysis. This interaction is mediated by the N-terminal domain of NSF. The E138G and DeltaI137 mutations of RP2 known to cause XLRP abolished the interaction of RP2 with the N-terminal domain of NSF. Immunofluorescence labeling studies further showed that RP2 colocalized with NSF in photoreceptors and other cells of the retina. Intense punctate staining of RP2 was observed close to the junction between the inner and outer segments beneath the connecting cilium, as well as within the synaptic region of rod and cone photoreceptors. Our studies indicate that RP2, in addition to serving as a regulator of Arl3, interacts with NSF, and this complex may play an important role in membrane protein trafficking in photoreceptors and other cells of the retina.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Holopainen JM,Cheng CL,Molday LL,Johal G,Coleman J,Dyka F,Hii T,Ahn J,Molday RS

doi

10.1021/bi1005249

subject

Has Abstract

pub_date

2010-09-07 00:00:00

pages

7439-47

issue

35

eissn

0006-2960

issn

1520-4995

journal_volume

49

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Binding of cytochrome P450 monooxygenase and lipoxygenase pathway products by heart fatty acid-binding protein.

    abstract：:Arachidonic acid metabolism by lipoxygenases and cytochrome P450 monooxygenases produces regioisomeric hydroperoxyeicosatetraenoic acids (HPETEs), hydroxyeicosatetraenoic acids (HETEs), epoxyeicosatrienoic acids (EETs), and dihydroxyeicosatrienoic acids (DHETs), which serve as components of cell signaling cascades. In...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001602y

    authors： Widstrom RL,Norris AW,Spector AA

    更新日期：2001-01-30 00:00:00

  • Interaction of tRNAs and of phosphorothioate-substituted nucleic acids with an organomercurial. Probing the chemical environment of thiolated residues by affinity electrophoresis.

    abstract：:The interactions of 4-thiouridine and 5-[(methylamino)methyl]-2-thiouridine in tRNA and of phosphorothioate esters in nucleic acids with an organomercurial have been investigated. For this purpose, an affinity electrophoretic system has been developed in which the mercury derivative has been covalently immobilized in ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00410a048

    authors： Igloi GL

    更新日期：1988-05-17 00:00:00

  • Self-assembly of aqueous bilirubin ditaurate, a natural conjugated bile pigment, to contraposing enantiomeric dimers and M(-) and P(+) tetramers and their selective hydrophilic disaggregation by monomers and micelles of bile salts.

    abstract：:The solution behavior of bilirubin ditaurate (BDT), the first naturally occurring conjugated bile pigment to be physically and chemically characterized, was assessed in aqueous solution and in monomeric and micellar solutions of common taurine-conjugated bile salts (BS). Analytical ultracentrifugation revealed that BD...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi501251v

    authors： Neubrand MW,Carey MC,Laue TM

    更新日期：2015-02-24 00:00:00

  • Destabilization of i-Motif DNA at Neutral pH by G-Quadruplex Ligands.

    abstract：:Numerous studies have been published stressing the importance of finding ligands that can bind specifically to DNA secondary structures. Several have identified ligands that are presented as having specific binding to the G-quadruplex; however, these were not originally tested on the complementary i-motif structure. T...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00968

    authors： Abdelhamid MAS,Gates AJ,Waller ZAE

    更新日期：2019-01-29 00:00:00

  • Cleavage of DNA by the insulin-mimetic compound, NH4[VO(O2)2(phen)].

    abstract：:The kinetics and mechanism of cleavage of DNA by the insulin-mimetic peroxo-vanadate NH4[VO(O2)2(phen)], pV, are described. In the presence of low energy UV radiation or biologically common reducing agents, pV decomposes into the monomer, dimer, and tetramer of vanadate and an uncharacterized compound of V4+ as shown ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9606253

    authors： Hiort C,Goodisman J,Dabrowiak JC

    更新日期：1996-09-24 00:00:00

  • Preparation and X-ray structures of metal-free, dicobalt and dimanganese forms of soluble methane monooxygenase hydroxylase from Methylococcus capsulatus (Bath).

    abstract：:A three-component soluble methane monooxygenase (sMMO) enzyme system catalyzes the hydroxylation of methane to methanol at a carboxylate-bridged diiron center housed in the alpha-subunit of the hydroxylase (MMOH). Catalysis is facilitated by the presence of a regulatory protein (MMOB) and inhibited by MMOD, a protein ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048140z

    authors： Sazinsky MH,Merkx M,Cadieux E,Tang S,Lippard SJ

    更新日期：2004-12-28 00:00:00

  • Kinetic characterization of recombinant human acidic mammalian chitinase.

    abstract：:Human acidic mammalian chitinase (AMCase), a member of the family 18 glycosyl hydrolases, is one of the important proteins involved in Th2-mediated inflammation and has been implicated in asthma and allergic diseases. Inhibition of AMCase results in decreased airway inflammation and airway hyper-responsiveness in a mo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0525977

    authors： Chou YT,Yao S,Czerwinski R,Fleming M,Krykbaev R,Xuan D,Zhou H,Brooks J,Fitz L,Strand J,Presman E,Lin L,Aulabaugh A,Huang X

    更新日期：2006-04-11 00:00:00

  • Role of proline residues in the structure and function of a membrane transport protein.

    abstract：:By use of site-directed mutagenesis, each prolyl residue in the lac permease of Escherichia coli at positions 28 (putative helix I), 31 (helix I), 61 (helix II), 89 (helix III), 97 (helix III), 123 (helix IV), 192 (putative hydrophilic region 7), 220 (helix VII), 280 (helix VIII), and 327 [helix X; Lolkema, J. S., et ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00219a019

    authors： Consler TG,Tsolas O,Kaback HR

    更新日期：1991-02-05 00:00:00

  • Casein kinase II phosphorylation of caldesmon downregulates myosin-caldesmon interactions.

    abstract：:It is well-known that caldesmon (CaD) is a substrate for casein kinase II (CKII), and the phosphorylation of CaD by CKII regulates the interaction of CaD with myosin. However, the functionally relevant CKII phosphorylation site(s) on CaD and the precise role of CaD phosphorylation by CKII in mediating CaD's function h...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0006767

    authors： Wang Z,Yang ZQ

    更新日期：2000-09-12 00:00:00

  • Mechanistic insights from the binding of substrate and carbocation intermediate analogues to aristolochene synthase.

    abstract：:Aristolochene synthase, a metal-dependent sesquiterpene cyclase from Aspergillus terreus, catalyzes the ionization-dependent cyclization of farnesyl diphosphate (FPP) to form the bicyclic eremophilane (+)-aristolochene with perfect structural and stereochemical precision. Here, we report the X-ray crystal structure of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi400691v

    authors： Chen M,Al-lami N,Janvier M,D'Antonio EL,Faraldos JA,Cane DE,Allemann RK,Christianson DW

    更新日期：2013-08-13 00:00:00

  • Redox-dependent structural differences in putidaredoxin derived from homologous structure refinement via residual dipolar couplings.

    abstract：:Structural differences in the [2Fe-2S] ferredoxin, putidaredoxin (Pdx), from the camphor hydroxylation pathway of Pseudomonas putida have been investigated as a function of oxidation state of the iron cluster. Pdx is involved in biological electron transfer to cytochrome P450(cam) (CYP101). Redox-dependent differences...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050152c

    authors： Jain NU,Tjioe E,Savidor A,Boulie J

    更新日期：2005-06-28 00:00:00

  • Mutations in the B12-binding region of methionine synthase: how the protein controls methylcobalamin reactivity.

    abstract：:Vitamin B12-dependent methionine synthase catalyzes the transfer of a methyl group from methyltetrahydrofolate to homocysteine via the enzyme-bound cofactor methylcobalamin. To carry out this reaction, the enzyme must alternately stabilize six-coordinate methylcobalamin and four-coordinate cob(I)alamin oxidation state...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi952389m

    authors： Jarrett JT,Amaratunga M,Drennan CL,Scholten JD,Sands RH,Ludwig ML,Matthews RG

    更新日期：1996-02-20 00:00:00

  • Levuglandin forms adducts with histone h4 in a cyclooxygenase-2-dependent manner, altering its interaction with DNA.

    abstract：:Inflammation and subsequent cyclooxygenase-2 (COX-2) activity has long been linked with the development of cancer, although little is known about any epigenetic effects of COX-2. A product of COX-2 activation, levuglandin (LG) quickly forms covalent bonds with nearby primary amines, such as those in lysine, which lead...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi401673b

    authors： Carrier EJ,Zagol-Ikapitte I,Amarnath V,Boutaud O,Oates JA

    更新日期：2014-04-22 00:00:00

  • The stereoselectivity and catalytic properties of Xanthobacter autotrophicus 2-[(R)-2-Hydroxypropylthio]ethanesulfonate dehydrogenase are controlled by interactions between C-terminal arginine residues and the sulfonate of coenzyme M.

    abstract：:2-[(R)-2-Hydroxypropylthio]ethanesulfonate (R-HPC) dehydrogenase (DH) catalyzes the reversible oxidation of R-HPC to 2-(2-ketopropylthio)ethanesulfonate (2-KPC) in a key reaction in the bacterial conversion of chiral epoxides to beta-keto acids. R-HPCDH is highly specific for the R-enantiomer of HPC, while a separate ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049783h

    authors： Clark DD,Boyd JM,Ensign SA

    更新日期：2004-06-01 00:00:00

  • Functional interactions in cytochrome P450BM3. Evidence that NADP(H) binding controls redox potentials of the flavin cofactors.

    abstract：:NADP(H) binding is essential for fast electron transfer through the flavoprotein domain of the fusion protein P450BM3. Here we characterize the interaction of NADP(H) with the oxidized and partially reduced enzyme and the effect of this interaction on the redox properties of flavin cofactors and electron transfer. Mea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001068u

    authors： Murataliev MB,Feyereisen R

    更新日期：2000-10-17 00:00:00

  • Polymorphism of F-actin assembly. 2. Effects of barbed end capping on F-actin assembly.

    abstract：:In the accompanying paper [Suzuki, A., Yamazaki, M., & Ito, T. (1996) Biochemistry 35, 5238-5244], we presented a quantitative phase diagram of actin filament ( (F-actin) described with the F-actin concentration and delta chi value which characterizes the affinity of F-actin with solvent. The phase diagram shows that ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9526948

    authors： Suzuki A,Ito T

    更新日期：1996-04-23 00:00:00

  • Defining the precision with which a protein structure is determined by NMR. Application to motilin.

    abstract：:A simple procedure is introduced for accurately defining the precision with which the Cartesian coordinates of any macromolecular structure are determined by nuclear Overhauser data. The method utilizes an ensemble of structures obtained from an array of independent simulated data sets derived from a final structure. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00057a027

    authors： Shriver J,Edmondson S

    更新日期：1993-02-16 00:00:00

  • Preparation and Application of 13C-Labeled myo-Inositol to Identify New Catabolic Products in Inositol Metabolism in Lactobacillus casei.

    abstract：:myo-Inositol (mI) is widely distributed in all domains of life and is important for several cellular functions, including bacterial survival. The enzymes responsible for the bacterial catabolism of mI, encoded in the iol operon, can vary from one organism to another, and these pathways have yet to be fully characteriz...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00539

    authors： Ramos-Figueroa JS,Aamudalapalli HB,Jagdhane RC,Smith J,Palmer DRJ

    更新日期：2020-08-18 00:00:00

  • Osmotic swelling allows fusion of sendai virions with membranes of desialyzed erythrocytes and chromaffin granules.

    abstract：:Sendai virus particles are able to fuse with Pronase-neuraminidase-treated human erythrocyte membranes as well as with vesicles obtained from chromaffin granules of bovine medulla. Fusion is inferred either from electron microscopic studies or from the observation that incubation of fluorescently labeled (bearing octa...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00387a018

    authors： Citovsky V,Laster Y,Schuldiner S,Loyter A

    更新日期：1987-06-30 00:00:00

  • X-ray spectroscopy of the iron site in soybean lipoxygenase-1: changes in coordination upon oxidation or addition of methanol.

    abstract：:Iron K-edge X-ray spectroscopy (XANES and EXAFS) was used to study iron coordination in frozen solutions of soybean lipoxygenase-1 (SLO). The intensity of the 1s-->3d pre-edge transition of native iron(II) lipoxygenase is greater than what was found for six-coordinate high-spin iron(II) model complexes, but comparable...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00254a011

    authors： Scarrow RC,Trimitsis MG,Buck CP,Grove GN,Cowling RA,Nelson MJ

    更新日期：1994-12-20 00:00:00

  • Elucidation of the structural basis for the slow reactivity of thrombin with plasminogen activator inhibitor-1.

    abstract：:Plasminogen activator inhibitor-1 (PAI-1) is a serine protease inhibitor of the serpin superfamily which rapidly inactivates tissue plasminogen activator (tPA), but reacts with thrombin at a much slower rate. Based on the previous mutagenesis studies and the X-ray crystal structure of the thrombin E192Q-bovine pancrea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9808518

    authors： Rezaie AR

    更新日期：1998-09-22 00:00:00

  • Crystal Structure of the Zorbamycin-Binding Protein ZbmA, the Primary Self-Resistance Element in Streptomyces flavoviridis ATCC21892.

    abstract：:The bleomycins (BLMs), tallysomycins (TLMs), phleomycin, and zorbamycin (ZBM) are members of the BLM family of glycopeptide-derived antitumor antibiotics. The BLM-producing Streptomyces verticillus ATCC15003 and the TLM-producing Streptoalloteichus hindustanus E465-94 ATCC31158 both possess at least two self-resistanc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b01008

    authors： Rudolf JD,Bigelow L,Chang C,Cuff ME,Lohman JR,Chang CY,Ma M,Yang D,Clancy S,Babnigg G,Joachimiak A,Phillips GN Jr,Shen B

    更新日期：2015-11-17 00:00:00

  • Activity-dependent phosphorylation of dynamin 1 at serine 857.

    abstract：:Dynamin 1 is thought to mediate synaptic transmission through interactions with multiple endocytic accessory proteins in a phosphorylation-dependent manner. Previously, we have shown that DYRK1A, a chromosome 21-encoded kinase implicated in the mental retardation of Down syndrome, phosphorylates primarily serine 857 (...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi2017798

    authors： Xie W,Adayev T,Zhu H,Wegiel J,Wieraszko A,Hwang YW

    更新日期：2012-08-28 00:00:00

  • New synthetic cluster ligands for galactose/N-acetylgalactosamine-specific lectin of mammalian liver.

    abstract：:Synthetic ligands containing up to six residues of nonreducing terminal galactose were prepared. The synthesis involved coupling of carboxyl groups of N-benzyloxy-carbonylaspartic acid or of N-benzyloxycarbonyltyrosyl-gamma-glutamylglutamic acid to the omega-amino group of the aglycon of a glycoside that contained up ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00313a037

    authors： Lee RT,Lin P,Lee YC

    更新日期：1984-08-28 00:00:00

  • Combinatorial optimization of the DNA cleaving Ni(II) x Xaa-Xaa-His metallotripeptide domain.

    abstract：:A positional-scanning combinatorial protocol was employed to optimize the deoxyribose-based cleavage of B-form DNA by Ni(II) x Xaa-Xaa-His metallopeptides. This procedure employed 18 naturally occurring amino acids (excluding Cys and Trp) to generate two libraries in which the first and second positions of the peptide...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi982587o

    authors： Huang X,Pieczko ME,Long EC

    更新日期：1999-02-16 00:00:00

  • Structural, dynamic, and chemical characterization of a novel S-glycosylated bacteriocin.

    abstract：:Bacteriocins are bacterial peptides with specific activity against competing species. They hold great potential as natural preservatives and for their probiotic effects. We show here nuclear magnetic resonance-based evidence that glycocin F, a 43-amino acid bacteriocin from Lactobacillus plantarum, contains two β-link...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200217u

    authors： Venugopal H,Edwards PJ,Schwalbe M,Claridge JK,Libich DS,Stepper J,Loo T,Patchett ML,Norris GE,Pascal SM

    更新日期：2011-04-12 00:00:00

  • Complete structure of the adhesin receptor polysaccharide of Streptococcus oralis ATCC 55229 (Streptococcus sanguis H1).

    abstract：:This report describes the determination of the complete primary structure of the adhesin receptor polysaccharide of Streptococcus oralis ATCC 55229 (previously characterized as Streptococcus sanguis H1), a Gram-positive bacteria implicated in dental plaque formation. The polysaccharide was isolated from S. oralis ATCC...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00159a014

    authors： Glushka J,Cassels FJ,Carlson RW,van Halbeek H

    更新日期：1992-11-10 00:00:00

  • Discovery of a novel L-lyxonate degradation pathway in Pseudomonas aeruginosa PAO1.

    abstract：:The l-lyxonate dehydratase (LyxD) in vitro enzymatic activity and in vivo metabolic function were assigned to members of an isofunctional family within the mandelate racemase (MR) subgroup of the enolase superfamily. This study combined in vitro and in vivo data to confirm that the dehydration of l-lyxonate is the bio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi5004298

    authors： Ghasempur S,Eswaramoorthy S,Hillerich BS,Seidel RD,Swaminathan S,Almo SC,Gerlt JA

    更新日期：2014-05-27 00:00:00

  • The SIV tilted peptide induces cylindrical reverse micelles in supported lipid bilayers.

    abstract：:Elucidation of the molecular mechanism leading to biomembrane fusion is a challenging issue in current biomedical research in view of its involvement in controlling cellular functions and in mediating various important diseases. According to the generally admitted stalk mechanism described for membrane fusion, negativ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060317x

    authors： El Kirat K,Dufrêne YF,Lins L,Brasseur R

    更新日期：2006-08-01 00:00:00

  • Site-directed mutagenesis and 1H NMR spectroscopy of an interdomain segment in the pyruvate dehydrogenase multienzyme complex of Escherichia coli.

    abstract：:Deletion of two of the three homologous lipoyl domains that form the N-terminal half of each dihydrolipoamide acetyltransferase (E2p) polypeptide chain of the Escherichia coli pyruvate dehydrogenase complex can be achieved by in vitro deletion in the structural gene aceF. A site-directed mutagenesis of this shortened ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00401a044

    authors： Texter FL,Radford SE,Laue ED,Perham RN,Miles JS,Guest JR

    更新日期：1988-01-12 00:00:00