Mutations in the B12-binding region of methionine synthase: how the protein controls methylcobalamin reactivity.

abstract：:The structural domains of human apolipoprotein(a) (apo(a)) in the lipoprotein(a) (Lp(a)) particle have been recently investigated by limited proteolysis [Huby, T., Doucet, C., Dieplinger, H., Chapman, J., & Thillet, J. (1994) Biochemistry 33, 3335-3341]. We have shown that apo(a) can be cleaved into two structural dom...

journal_title：Biochemistry

authors： Huby T,Schröder W,Doucet C,Chapman J,Thillet J

更新日期：1995-06-06 00:00:00

abstract：:A 15-residue fragment within the major continuous domain of gp120 from HIV-1 that can bind independently to the CD4 receptor has been shown to have the property of behaving as a solvent polarity-dependent conformational switch. The switch behavior (cooperative transition from beta-sheet to helical conformation as a fu...

journal_title：Biochemistry

authors： Graf von Stosch A,Kinzel V,Reed J

更新日期：1996-01-16 00:00:00

abstract：:To avoid nonspecific and/or undesirable binding and reactivity of metal ions with cellular components, organisms have evolved metal-specific systems for trafficking proteins. Although systems differ, those handling soft metal ions such as Hg(2+), Cu(+), Zn(2+), etc., all utilize heavy metal-associated (HMA) proteins a...

journal_title：Biochemistry

authors： Ledwidge R,Hong B,Dötsch V,Miller SM

更新日期：2010-10-19 00:00:00

abstract：:DNA topoisomerase I (Top1) is an essential nuclear enzyme and a validated target for anticancer agent screening. In a previous study, we found that indolizinoquinoline-5,12-dione derivatives show significant biological activity against several human cancer cell lines. To understand their mechanism of inhibition of can...

journal_title：Biochemistry

authors： Wu N,Wu XW,Agama K,Pommier Y,Du J,Li D,Gu LQ,Huang ZS,An LK

更新日期：2010-11-30 00:00:00

abstract：:Thioltransferase activity was identified and the enzyme purified to apparent homogeneity from human red blood cells. Activity was measured as glutathione-dependent reduction of the prototype substrate hydroxyethyl disulfide; formation of oxidized glutathione (GSSG) was coupled to NADPH oxidation by GSSG reductase (1 u...

journal_title：Biochemistry

authors： Mieyal JJ,Starke DW,Gravina SA,Dothey C,Chung JS

更新日期：1991-06-25 00:00:00

abstract：:The inhibitory effects of guanosine 5'-(gamma-fluorotriphosphate) [GTP(gamma F)] on both the polymerization and the colchicine-dependent GTPase activity of calf brain tubulin have been studied. The results demonstrate that this analogue of GTP, with a fluorine atom on the gamma-phosphate, is a reversible competitive d...

journal_title：Biochemistry

authors： Monasterio O,Timasheff SN

更新日期：1987-09-22 00:00:00

abstract：:Native EcoRI DNA methyltransferase (Mtase, Mr 38,050) is proteolyzed by trypsin to generate an intermediate 36-kDa fragment (p36) followed by the formation of two polypeptides of Mr 23,000 and 13,000 (p23 and p13, respectively). Protein sequence analysis of the tryptic fragments indicates that p36 results from removal...

journal_title：Biochemistry

authors： Reich NO,Maegley KA,Shoemaker DD,Everett E

更新日期：1991-03-19 00:00:00

abstract：:NMR spectroscopic experiments were performed to study the structure of synthetic peptides identical to two extracellular regions of the human thrombin receptor. The smaller molecule, comprising 14 amino acids, was biologically active and was equivalent to the "tethered ligand" exposed after cleavage of the receptor by...

journal_title：Biochemistry

authors： Smith KJ,Trayer IP,Grand RJ

更新日期：1994-05-24 00:00:00

abstract：:Ribonucleotide reductase from Escherichia coli consists of two nonidentical subunits, named protein B1 (170 000) and protein B2 (87 000). We purified and characterized five monoclonal antibodies against B1 and three against B2 from hybridomas obtained by fusion of spleen cells from immunized mice and the myeloma cell ...

journal_title：Biochemistry

authors： Anderson A,Barlow T,Pontis E,Reichard P

更新日期：1986-02-25 00:00:00

abstract：:Alpha2u-globulin is a male rat liver protein under multihormonal control which represents approximately 1% of hepatic protein synthesis. We have measured the rate of transcription of the alpha2u-globulin gene using a nuclear cell-free transcriptional system with mercurated CTP as substrate for the endogenous RNA polym...

journal_title：Biochemistry

authors： Chan KM,Kurtz DT,Feigelson P

更新日期：1978-07-25 00:00:00

abstract：:The heterotrimeric G protein subunit, alpha(s), can move reversibly from plasma membranes to cytoplasm in response to activation by GPCRs or activating mutations. We examined the importance of the unique N-terminus of alpha(s) in this translocation in cultured cells. alpha(s) contains a single site for palmitoylation ...

journal_title：Biochemistry

authors： Thiyagarajan MM,Bigras E,Van Tol HH,Hébert TE,Evanko DS,Wedegaertner PB

更新日期：2002-07-30 00:00:00

abstract：:Protein phosphatase 1 (PP1) is an essential and ubiquitous serine/threonine protein phosphatase that is regulated by more than 100 known inhibitor and targeting proteins. It is currently unclear how protein inhibitors distinctly and specifically regulate PP1 to enable rapid responses to cellular alterations. We demons...

journal_title：Biochemistry

authors： Dancheck B,Nairn AC,Peti W

更新日期：2008-11-25 00:00:00

abstract：:Succinyl-CoA:3-oxoacid coenzyme A transferase (EC 2.8.3.5), which catalyzes the reversible conversion of succinyl-CoA and acetoacetate into acetoacetyl-CoA and succinate through a covalent enzyme thiol ester intermediate, E-CoA, utilizes binding energy from noncovalent interactions with CoA to bring about an increase ...

journal_title：Biochemistry

authors： Whitty A,Fierke CA,Jencks WP

更新日期：1995-09-19 00:00:00

abstract：:The occurrence of 8-oxo-7,8-dihydroguanine (OG) in the genome, as one of the major DNA oxidative damages, has been implicated in an array of biological processes, ranging from mutagenesis to transcriptional regulation. Genome-wide mapping of oxidative damages could shed light on the underlying cellular mechanism. In t...

journal_title：Biochemistry

authors： Fang Y,Zou P

更新日期：2020-01-14 00:00:00

abstract：:Peptidylglycine monooxygenase (PHM) catalyzes the final step in the biosynthesis of amidated peptides that serve as important signaling molecules in numerous endocrine pathways. The catalytic mechanism has attracted much attention because of a number of unique attributes, including the presence of a pair of uncoupled ...

journal_title：Biochemistry

authors： Chauhan S,Kline CD,Mayfield M,Blackburn NJ

更新日期：2014-02-18 00:00:00

abstract：:The biodegradative 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase of Pseudomonas mevalonii catalyzes the NAD(+)-dependent conversion of (S)-HMG-CoA to (R)-mevalonate. Crystallographic analysis of abortive ternary complexes of this enzyme revealed lysine 267 located at a position in the active site, suggesti...

journal_title：Biochemistry

authors： Bochar DA,Tabernero L,Stauffacher CV,Rodwell VW

更新日期：1999-07-13 00:00:00

abstract：:The dynamic equilibrium of a catalytic site between active and inactive conformations, the missing link between the structure and function of allosteric enzymes, was identified using protein engineering and NMR techniques. Kinetic analyses of the wild-type and three mutants of Thermus L-lactate dehydrogenase establish...

journal_title：Biochemistry

authors： Koide S,Yokoyama S,Matsuzawa H,Miyazawa T,Ohta T

更新日期：1992-06-16 00:00:00

abstract：:The Wiskott-Aldrich syndrome protein (WASP) and neural WASP (N-WASP) are key players in regulating actin cytoskeleton via the Arp2/3 complex. It has been widely reported that the WASP proteins are activated by Rho family small GTPase Cdc42 and that Rac1 acts through SCAR/WAVE proteins. However, a systematic study of t...

journal_title：Biochemistry

authors： Tomasevic N,Jia Z,Russell A,Fujii T,Hartman JJ,Clancy S,Wang M,Beraud C,Wood KW,Sakowicz R

更新日期：2007-03-20 00:00:00

abstract：:The nuclear transport of the chromatin remodeling protein nucleoplasmin and chromatin building histones is mediated by importins. Nucleoplasmin (NP) contains a classical bipartite nuclear localization signal (NLS) that is recognized by the importin α/β heterodimer, while histones present multiple NLS-like motifs that ...

journal_title：Biochemistry

authors： Arregi I,Falces J,Bañuelos S,Urbaneja MA,Taneva SG

更新日期：2011-08-23 00:00:00

abstract：:The amino acid sequence of human alpha-fetoprotein, a 67-kDa protein present in mammalian embryonic serum, was verified by fast atom bombardment mass spectrometric (FAB/MS) analyses of three different enzymatic digests of the protein. Human alpha-fetoprotein obtained from a large-scale cell culture was digested with t...

journal_title：Biochemistry

authors： Pucci P,Siciliano R,Malorni A,Marino G,Tecce MF,Ceccarini C,Terrana B

更新日期：1991-05-21 00:00:00

abstract：:Crystals of the Y195F mutant of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 were subjected to a double soaking procedure, in which they were first soaked in a solution containing the inhibitor acarbose and subsequently in a solution containing maltohexaose. The refined structure of the resultin...

journal_title：Biochemistry

authors： Strokopytov B,Knegtel RM,Penninga D,Rozeboom HJ,Kalk KH,Dijkhuizen L,Dijkstra BW

更新日期：1996-04-02 00:00:00

abstract：:The thermotropic behavior of 20 chemically related glycosphingolipids (GSLs) of high purity, containing neutral and anionic carbohydrate residues in their oligosaccharide chains, was studied by high-sensitivity differential scanning calorimetry. In general, the polar head group of GSLs appears to be one of the major d...

journal_title：Biochemistry

authors： Maggio B,Ariga T,Sturtevant JM,Yu RK

更新日期：1985-02-26 00:00:00

abstract：:The high molecular weight folate binding protein of rat liver cytosol has been purified to apparent homogeneity. Purification was achieved by using a combination of gel filtration, O-(diethylaminoethyl)cellulose chromatography, and affinity chromatography. This folate binding protein was initially identified during pu...

journal_title：Biochemistry

authors： Cook RJ,Wagner C

更新日期：1982-08-31 00:00:00

abstract：:The arrangement of the mRNA on the Escherichia coli ribosome with respect to ribosomal RNA sites has been investigated by photochemical cross-linking experiments. mRNA analogues 51-54 nucleotides in length contained a Shine-Dalgarno sequence, a single codon for tRNA(Gly), and 4-thiouridine (s4U) in the 5' third of the...

journal_title：Biochemistry

authors： Bhangu R,Juzumiene D,Wollenzien P

更新日期：1994-03-15 00:00:00

abstract：:Bactenecin 5 and its fragments [BN22 (1-22), BN16 (7-22), and BC24 (20-43)] were synthesized by solid-phase methods. Their antifungal activities on Candida albicans have been studied and compared with those of the native bactenecin 5. The conformational preferences of these peptides in aqueous and nonaqueous solutions...

journal_title：Biochemistry

authors： Raj PA,Marcus E,Edgerton M

更新日期：1996-04-09 00:00:00

abstract：:The mechanisms by which multisubunit histone acetyltransferase (HAT) complexes recognize and perform efficient acetylation on nucleosome substrates are largely unknown. Here, we use a variety of biochemical approaches and compare histone-based substrates of increasing complexity to determine the critical components of...

journal_title：Biochemistry

authors： Berndsen CE,Selleck W,McBryant SJ,Hansen JC,Tan S,Denu JM

更新日期：2007-02-27 00:00:00

abstract：:We have developed an assay to continuously monitor the branched amino acid preferring peptidase (BrAAP) activity of the proteasome. This assay is based on the hydrolysis of the fluorogenic peptide, Abz-Gly-Pro-Ala-Leu-Ala-Nba (Abz is 2-aminobenzoyl and Nba is 4-nitrobenzylamide) which is cleaved exclusively at the Leu...

journal_title：Biochemistry

authors： McCormack TA,Cruikshank AA,Grenier L,Melandri FD,Nunes SL,Plamondon L,Stein RL,Dick LR

更新日期：1998-05-26 00:00:00

abstract：:The dynamic cytoskeletal network of microtubules and actin filaments can be disassembled by drugs. Cytoskeletal drugs work by perturbing the monomer-polymer equilibrium, thus changing the size and number of macromolecular crowders inside cells. Changes in both crowding and nonspecific surface interactions ("sticking")...

journal_title：Biochemistry

authors： Davis CM,Gruebele M

更新日期：2020-07-21 00:00:00

abstract：:N-Linked sugar chains were liberated by hydrazinolysis from porcine zona pellucida glycoproteins obtained from ovarian follicular oocytes. Neutral sugar chains were separated from acidic ones by paper electrophoresis and fractionated with a serial lectin column chromatography and Bio-Gel P-4 column chromatography. The...

journal_title：Biochemistry

authors： Mori E,Takasaki S,Hedrick JL,Wardrip NJ,Mori T,Kobata A

更新日期：1991-02-26 00:00:00

abstract：:Previous optical and electron paramagnetic resonance (EPR) spectroscopic studies of the newly discovered peroxynitrite scavenging pseudoperoxidase from Leishmania major (LmPP) suggested that ferric LmPP contained a six-coordinate low-spin (6cLS) heme with a thiolate ligand, presumably a cysteine, bound to its heme iro...

journal_title：Biochemistry

authors： Saha R,Bose M,Sen Santara S,Roy J,Adak S

更新日期：2013-12-10 00:00:00