Arrangement of messenger RNA on Escherichia coli ribosomes with respect to 10 16S rRNA cross-linking sites.

abstract：:The structural basis for the improvement in catalytic efficiency of the mutant E165D chicken triosephosphate isomerase by the secondary mutation, S96P, has been analyzed using a combination of X-ray crystallography and Fourier transform infrared spectroscopy. All X-ray structures were of the complex of phosphoglycoloh...

journal_title：Biochemistry

authors： Komives EA,Lougheed JC,Liu K,Sugio S,Zhang Z,Petsko GA,Ringe D

更新日期：1995-10-17 00:00:00

abstract：:6-Hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes the pyrophosphoryl transfer from ATP to 6-hydroxymethyl-7,8-dihydropterin (HP), the first reaction in the folate biosynthetic pathway. Arginine residues 82 and 92, strictly conserved in 35 HPPK sequences, play dynamic roles in the catalytic cycle of ...

journal_title：Biochemistry

authors： Blaszczyk J,Li Y,Shi G,Yan H,Ji X

更新日期：2003-02-18 00:00:00

abstract：:In bacteria, regulatory phosphorylation of proteins at serine and/or threonine residues by Ser/Thr protein kinase (STPK) is an emerging theme in prokaryotic signaling, particularly since the prediction of the occurrence of several STPKs from genome sequencing and sequence surveys. Here we show that protein PknH posses...

journal_title：Biochemistry

authors： Molle V,Kremer L,Girard-Blanc C,Besra GS,Cozzone AJ,Prost JF

更新日期：2003-12-30 00:00:00

abstract：:The complete amino acid sequence of a small, basic protein with cardiotoxic activity is described. This toxin, designated Naja naja F8, was isolated from the venom of Naja naja, of Cambodian origin, by gel filtration on Sephadex G-75 followed by gradient ion exchange chromatography on Bio-Rex 70. The cardiotoxin F8, m...

journal_title：Biochemistry

authors： Fryklund L,Eaker D

更新日期：1975-07-01 00:00:00

abstract：:For many different enveloped viruses the crystal structure of the fusion protein core has been established. A striking conservation in the tertiary and quaternary arrangement of these core structures is repeatedly revealed among members of diverse families. It has been proposed that the primary role of the core involv...

journal_title：Biochemistry

authors： Sackett K,Shai Y

更新日期：2002-04-09 00:00:00

abstract：:Potent inhibitors of human cysteine proteases of the papain family have been made and assayed versus a number of relevant family members. We describe the synthesis of peptide alpha-ketoheterocyclic inhibitors that occupy binding subsites S1'-S3 of the cysteine protease substrate recognition cleft and that form a rever...

journal_title：Biochemistry

authors： McGrath ME,Sprengeler PA,Hill CM,Martichonok V,Cheung H,Somoza JR,Palmer JT,Janc JW

更新日期：2003-12-30 00:00:00

abstract：:The electron density map of the 3D crystal of Photosystem II from Thermosynechococcus vulcanus with a 1.9 Å resolution (PDB: 3ARC ) exhibits, in the two monomers in the asymmetric unit cell, an, until now, unidentified and uninterpreted strong difference in electron density centered at a distance of around 1.5 Å from ...

journal_title：Biochemistry

authors： Sugiura M,Koyama K,Umena Y,Kawakami K,Shen JR,Kamiya N,Boussac A

更新日期：2013-12-31 00:00:00

abstract：:The crystal structure of fiddler crab collagenase complexed with the dimeric serine protease inhibitor ecotin at 2.5 A resolution reveals an extended cleft providing binding sites for at least 11 contiguous substrate residues. Comparison of the positions of nine intermolecular main chain hydrogen bonding interactions ...

journal_title：Biochemistry

authors： Perona JJ,Tsu CA,Craik CS,Fletterick RJ

更新日期：1997-05-06 00:00:00

abstract：:The ribosome-dependent stringent factor reaction was found to be nonspecific with regard to the number of phosphate groups linked to the 5' position of guanosine nucleotides. Both GMP and guanosine 5'-tetraphosphate could accept a pyrophosphoryl group from ATP although at a much lower rate than GDP or GTP. Guanosine 5...

journal_title：Biochemistry

authors： Sy J

更新日期：1975-03-11 00:00:00

abstract：:X-ray crystal structures of glutamine-dependent amidotransferases in their "active" conformation have revealed the existence of multiple active sites linked by solvent inaccessible intramolecular channels, giving rise to the widely accepted view that ammonia released in a glutaminase site is channeled efficiently into...

journal_title：Biochemistry

authors： Li KK,Beeson WT 4th,Ghiviriga I,Richards NG

更新日期：2007-04-24 00:00:00

abstract：:Chicken nucleoside triphosphate diphosphohydrolase 8 (NTPDase8) is a cell surface ectonucleotidase with a large extracellular domain (ECD) containing the active site and is anchored to the membrane by two transmembrane domains (TMDs) at the N- and C-termini. Unlike other cell surface NTPDases that have been characteri...

journal_title：Biochemistry

authors： Li CS,Lee Y,Knowles AF

更新日期：2010-01-12 00:00:00

abstract：:We have biochemically and kinetically characterized the polymerase and exonuclease activities of the third B-family polymerase (Dpo3) from the hyperthermophilic Crenarchaeon, Sulfolobus solfataricus (Sso). We have established through mutagenesis that despite incomplete sequence conservation, the polymerase and exonucl...

journal_title：Biochemistry

authors： Bauer RJ,Begley MT,Trakselis MA

更新日期：2012-03-06 00:00:00

abstract：:Murine class alpha glutathione S-transferase subunit types A2 (mGSTA2-2) and A1 (mGSTA1-1) have high catalytic efficiency for glutathione (GSH) conjugation of the ultimate carcinogenic metabolite of benzo[a]pyrene, (+)-anti-7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene, [(+)-anti-BPDE]. Only 10 residues dif...

journal_title：Biochemistry

authors： Gu Y,Xiao B,Wargo HL,Bucher MH,Singh SV,Ji X

更新日期：2003-02-04 00:00:00

abstract：:The human transferrin receptor (TfR) has three N-linked oligosaccharides. A combination of site-directed mutagenesis and carbohydrate and protein chemistry was used to characterize the structures of the N-linked oligosaccharides and to map their locations. We find that the type of oligosaccharide at each position was ...

journal_title：Biochemistry

authors： Hayes GR,Williams AM,Lucas JJ,Enns CA

更新日期：1997-04-29 00:00:00

abstract：:A new fluorescence method to measure viscosity in cell cytosol [Fushimi, K., & Verkman, A. S. (1991) J. Cell Biol. 112, 719-725] has been applied to determine fluid-phase viscosity in sea urchin eggs. Freshly harvested eggs from Lytechinus pictus were loaded with the dyes 2,7-bis(2-carboxyethyl)-5-(and-6-)carboxyfluor...

journal_title：Biochemistry

authors： Periasamy N,Armijo M,Verkman AS

更新日期：1991-12-24 00:00:00

abstract：:The oxygen transfer to p-hydroxybenzoate catalyzed by p-hydroxybenzoate hydroxylase (PHBH) has been shown to occur via a C4a-hydroperoxide of the flavin. Two factors are likely to be important in facilitating the transfer of oxygen from the C4a-hydroperoxide to the substrate. (a) The positive electrostatic potential o...

journal_title：Biochemistry

authors： Ortiz-Maldonado M,Aeschliman SM,Ballou DP,Massey V

更新日期：2001-07-31 00:00:00

abstract：:There are highly conserved hydrogen bonds between the distal His and the adjacent Asn in many peroxidases. Although the crystal structure of horseradish peroxidase C (HRP) is not available, comparison of the amino acid sequence with cytochrome c peroxidase indicates that Asn70 is making the hydrogen bond with the dist...

journal_title：Biochemistry

authors： Nagano S,Tanaka M,Ishimori K,Watanabe Y,Morishima I

更新日期：1996-11-12 00:00:00

abstract：:Nephrocalcin (NC), a calcium-binding glycoprotein of 14,000 molecular weight as a monomer, is known to inhibit the growth of calcium oxalate monohydrate (COM) crystals in renal tubules. We have isolated NC from bovine kidney tissue and purified into four isoforms, fractions A-D. NC-A and NC-B strongly inhibit the grow...

journal_title：Biochemistry

authors： Mustafi D,Nakagawa Y

更新日期：1996-11-26 00:00:00

abstract：:There is considerable evidence that long-range interactions stabilize residual protein structure under denaturing conditions. However, evaluation of the effect of a specific contact on structure in the denatured state has been difficult. Iso-1-cytochrome c variants with a Lys54 → His mutation form a particularly stabl...

journal_title：Biochemistry

authors： Danielson TA,Stine JM,Dar TA,Briknarova K,Bowler BE

更新日期：2017-12-26 00:00:00

abstract：:Perturbations within the transactivation and carboxy-terminal domains of HIV-1 Rev were examined for effects on Rev responsive element (RRE) binding activities in vitro and biological activity in vivo. Binding affinities, specificities, and multimerization of the transactivation mutants M10 and Rev/Rex M10-16 on the R...

journal_title：Biochemistry

authors： Daly TJ,Rennert P,Lynch P,Barry JK,Dundas M,Rusche JR,Doten RC,Auer M,Farrington GK

更新日期：1993-08-31 00:00:00

abstract：:Class A beta-lactamases hydrolyze penicillins and other beta-lactams via an acyl-enzyme catalytic mechanism. Ser70 is the active site nucleophile. By constructing the S70A mutant, which is unable to form the acyl-enzyme intermediate, it was possible to make stable ES complexes with various substrates. The stability of...

journal_title：Biochemistry

authors： Hokenson MJ,Cope GA,Lewis ER,Oberg KA,Fink AL

更新日期：2000-05-30 00:00:00

abstract：:A dark state tertiary structure in the cytoplasmic domain of rhodopsin is presumed to be the key to the restriction of binding of transducin and rhodopsin kinase to rhodopsin. Upon light-activation, this tertiary structure undergoes a conformational change to form a new structure, which is recognized by the above prot...

journal_title：Biochemistry

authors： Klein-Seetharaman J,Hwa J,Cai K,Altenbach C,Hubbell WL,Khorana HG

更新日期：2001-10-23 00:00:00

abstract：:Thermal perturbation of the dicluster ferredoxin from Acidianus ambivalens was investigated employing a toolbox of spectroscopic methods. FTIR and visible CD were used for assessing changes of the secondary structure and coarse alterations of the [3Fe4S] and [4Fe4S] cluster moieties, respectively. Fine details of the ...

journal_title：Biochemistry

authors： Todorovic S,Leal SS,Salgueiro CA,Zebger I,Hildebrandt P,Murgida DH,Gomes CM

更新日期：2007-09-18 00:00:00

abstract：:Recognition of and binding to cholesterol on the host cell membrane is an initial step in the mechanism of numerous pathogens, including viruses, bacteria, and bacterial toxins; however, a viable method of inhibiting this interaction has not yet been uncovered. Here, we describe the mechanism by which a cholesterol re...

journal_title：Biochemistry

authors： Koufos E,Chang EH,Rasti ES,Krueger E,Brown AC

更新日期：2016-08-30 00:00:00

abstract：:Postsynaptic polypeptide neurotoxins isolated from the venoms of elapid and hydrophid snakes exhibit the ability to bind selectively to and inhibit different types of receptors that function in nerve signal transmission. On the basis of their amino acid sequences and three-dimensional structures, these neurotoxins are...

journal_title：Biochemistry

authors： Fiordalisi JJ,al-Rabiee R,Chiappinelli VA,Grant GA

更新日期：1994-04-05 00:00:00

abstract：:Two scorpion neurotoxins active only on insects, the insect toxins AaH IT1 and AaH IT2, were purified from the venom of scorpions Androctonus australis Hector collected in Tozeur (Tunisia) and characterized. AaH IT2 was sequenced and found to differ in four amino acid positions from AaH IT, the single previously seque...

journal_title：Biochemistry

authors： Loret EP,Mansuelle P,Rochat H,Granier C

更新日期：1990-02-13 00:00:00

abstract：:Histone redistributions between endogenous DNA in calf thymus chromatin and exogenous DNA from Clostridium perfringens (69% A + T) or from Micrococcus luteus (30% A + T) induced by 0.6 M NaCl or by 2% formaldehyde were studied by thermal denaturation. The observed redistribution occurred on histone Hl when the exogeno...

journal_title：Biochemistry

authors： Polacow I,Cabasso L,Li HJ

更新日期：1976-10-19 00:00:00

abstract：:Previous studies have shown that the short-motif electroplax Na channel is phosphorylated in vitro by cyclic AMP-dependent protein kinase (PKA) at serines 6 or 7 and 1776 and threonine 17 (Emerick & Agnew, 1989). We here show that phosphatase treatment of solubilized, purified Na channels enhanced subsequent PKA label...

journal_title：Biochemistry

authors： Emerick MC,Shenkel S,Agnew WS

更新日期：1993-09-14 00:00:00

abstract：:Single lamellar phosphatidyl[methyl-2H]choline vesicles were incubated with an excess of unlabeled phosphatidylcholine vesicles or phosphatidylcholine-cholesterol vesicles containing 8 mol % glucuronosyldiglyceride. Incubation of the two vesicle populations was performed in the presence or absence of a purified phosph...

journal_title：Biochemistry

authors： Shaw JM,Thompson TE

更新日期：1982-03-02 00:00:00

abstract：:Light-induced GTP-dependent scattering changes are studied in suspensions of retinal disc membranes to which one or both of the purified proteins involved in the phototransduction mechanism (G-protein and cGMP phosphodiesterase) are reassociated; a scattering change which depends on the presence of both G-protein (G) ...

journal_title：Biochemistry

authors： Bennett N,Clerc A

更新日期：1992-02-18 00:00:00