A convenient gHMQC-based NMR assay for investigating ammonia channeling in glutamine-dependent amidotransferases: studies of Escherichia coli asparagine synthetase B.

abstract：:Transferrin exhibits heterogeneity in glycosylation characteristic of pathological changes in alcohol abuse and congenital disorders in glycosylation. This study investigated an alternative approach in the detection of carbohydrate-deficient transferrin based on the premise that glycosylation may afford some degree of...

journal_title：Biochemistry

authors： Valmu L,Kalkkinen N,Husa A,Rye PD

更新日期：2005-12-13 00:00:00

abstract：:The side chains of R269 and N270 interact with the phosphodianion of dihydroxyacetone phosphate (DHAP) bound to glycerol 3-phosphate dehydrogenase (GPDH). The R269A, N270A, and R269A/N270A mutations of GPDH result in 9.1, 5.6, and 11.5 kcal/mol destabilization, respectively, of the transition state for GPDH-catalyzed ...

journal_title：Biochemistry

authors： Reyes AC,Amyes TL,Richard JP

更新日期：2016-03-15 00:00:00

abstract：:The primary structure of the 43-kilodalton peripheral membrane protein (43-kDa protein) of Torpedo nicotinic postsynaptic membrane has been determined. The 43-kDa protein, which was isolated by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis, has an amino terminus resistant to Edman degradation, ...

journal_title：Biochemistry

authors： Carr C,McCourt D,Cohen JB

更新日期：1987-11-03 00:00:00

abstract：:Under mildly acidic conditions, annexin 12 (ANX) inserts into lipid membranes to form a transbilayer pore [Langen, R., et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 14060]. In this study, we have addressed the question of the oligomeric state of ANX in this transbilayer conformation by means of Forster-type resonanc...

journal_title：Biochemistry

authors： Ladokhin AS,Haigler HT

更新日期：2005-03-08 00:00:00

abstract：:The ionization and phase behavior of 1,2-dipalmitoyl-sn-glycero-3-phosphoserine have been investigated under a variety of condtions by several different methods. As measured by turbidity changes, the temperature of the crystal-liquid crystal phase transition of this lipid is influenced by pH and mono- and divalent ca...

journal_title：Biochemistry

authors： MacDonald RC,Simon SA,Baer E

更新日期：1976-02-24 00:00:00

abstract：:A short chain dehydrogenase like l-threonine 3-dehydrogenase (SDR-TDH) from metagenome data (mtTDH) was identified by database mining. Its enzymatic properties suggested that mtTDH has unique characteristics relative to other SDR-TDHs, including two mesophilic and thermophilic SDR-TDHs identified in this study. The ac...

journal_title：Biochemistry

authors： Motoyama T,Nakano S,Yamamoto Y,Tokiwa H,Asano Y,Ito S

更新日期：2017-10-31 00:00:00

abstract：:The structural determinants of substrate specificity of human liver cytochrome P450 2C8 (CYP2C8) were investigated using site-directed mutants chosen on the basis of a preliminary substrate pharmacophore and a three-dimensional (3D) model. Analysis of the structural features common to CYP2C8 substrates exhibiting a mi...

journal_title：Biochemistry

authors： Melet A,Marques-Soares C,Schoch GA,Macherey AC,Jaouen M,Dansette PM,Sari MA,Johnson EF,Mansuy D

更新日期：2004-12-14 00:00:00

abstract：:We have studied the role of amphipathic alpha-helices in the ability of apoA-I to promote cholesterol efflux from human skin fibroblasts and activate lecithin:cholesterol acyltransferase (LCAT). Three apoA-I mutants were designed, each by deletion of a pair of predicted adjacent central alpha-helices [Delta(100-143), ...

journal_title：Biochemistry

authors： Frank PG,N'Guyen D,Franklin V,Neville T,Desforges M,Rassart E,Sparks DL,Marcel YL

更新日期：1998-09-29 00:00:00

abstract：:Chorismate-utilizing enzymes catalyze diverse reactions, providing critical physiological functions unique to plants, bacteria, fungi, and some parasites. Their absence in animals makes them excellent targets for antimicrobials and herbicides. 4-Amino-4-deoxychorismate synthase (ADCS) catalyzes the first step in folat...

journal_title：Biochemistry

authors： He Z,Toney MD

更新日期：2006-04-18 00:00:00

abstract：:Escherichia coli PurK, a dimeric N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) synthetase, catalyzes the conversion of 5-aminoimidazole ribonucleotide (AIR), ATP, and bicarbonate to N5-CAIR, ADP, and Pi. Crystallization of both a sulfate-liganded and the MgADP-liganded E. coli PurK has resulted in structures at 2....

journal_title：Biochemistry

authors： Thoden JB,Kappock TJ,Stubbe J,Holden HM

更新日期：1999-11-23 00:00:00

abstract：:Substitution of cysteine for proline-39 in Escherichia coli dihydrofolate reductase by oligonucleotide-directed mutagenesis positions the new cysteine adjacent to already existing cysteine-85. When the mutant protein is expressed in the E. coli cytosol, the cysteine sulfur atoms are found, by X-ray crystallographic an...

journal_title：Biochemistry

authors： Villafranca JE,Howell EE,Oatley SJ,Xuong NH,Kraut J

更新日期：1987-04-21 00:00:00

abstract：:Alignment of more than 20 deduced sequences for mevalonate diphosphate decarboxylase (MDD) indicates that serines 34, 36, 120,121, 153, and 155 are invariant residues that map within a proposed interdomain active site cleft. To test possible active site roles for these invariant serines, each has been mutated to alani...

journal_title：Biochemistry

authors： Krepkiy DV,Miziorko HM

更新日期：2005-02-22 00:00:00

abstract：:Deuterium magnetic resonance spectra (55.26 MHz) of cholesterol-3 alpha-d1 and epicholesterol-3 beta-d1 in dipalmitoylglycerophosphocholine (DPPC) liposomes were measured as a function of sterol-to-phospholipid ratio below (24 degrees C) and above (60 degrees C) the phase transition temperature of DPPC. From the quadr...

journal_title：Biochemistry

authors： Murari R,Murari MP,Baumann WJ

更新日期：1986-03-11 00:00:00

abstract：:Sedimentation and gel retardation studies show a stronger interaction of HMG 1 and 2 with negatively supercoiled DNA than with linear, nicked-circular, or positively supercoiled ds-DNA. An apparent unwinding angle of 58 degrees was obtained for HMG 1 and 2 when assayed by protection of negatively supercoiled DNA from ...

journal_title：Biochemistry

authors： Sheflin LG,Fucile NW,Spaulding SW

更新日期：1993-04-06 00:00:00

abstract：:Previously, we have described significant effects of human insulin on glucose metabolism in the yeast Saccharomyces cerevisiae under conditions of growth limitation. These regulations apparently rely on a transmembrane receptor capable of binding human insulin and responding by tyrosine/serine phosphorylation of a spe...

journal_title：Biochemistry

authors： Müller G,Grey S,Jung C,Bandlow W

更新日期：2000-02-15 00:00:00

abstract：:The Mn4 complex which is involved in water oxidation in photosystem II (PSII) is known to exhibit two types of EPR signals in the S2 state, one of the five redox states of the enzyme cycle: either a multiline signal (S = 1/2) or a signal at g = 4.1 (S = 3/2 or S= 5/2). The S = 1/2 state can be converted to that respon...

journal_title：Biochemistry

authors： Boussac A,Un S,Horner O,Rutherford AW

更新日期：1998-03-24 00:00:00

abstract：:Mercury ions can inhibit Candida albicans phosphomannose isomerase (PMI) by two different processes at sub-micromolar concentrations. Kinetic studies show that mercury ions are in rapid equilibrium with the enzyme and cause a clear partial noncompetitive inhibition when mannose 6-phosphate is used as the substrate. Th...

journal_title：Biochemistry

authors： Wells TN,Payton MA,Proudfoot AE

更新日期：1994-06-21 00:00:00

abstract：:H1 can rearrange in chicken erythrocyte polynucleosomes in 80 mM NaCl buffers. These rearrangements have been studied by sedimentation analysis. H1 redistributes between polynucleosomes as well as between polynucleosomes and monosomes. In these rearrangements H1 molecules move to free DNA sites. In contrast to H1, the...

journal_title：Biochemistry

authors： Lasters I,Muyldermans S,Wyns L,Hamers R

更新日期：1981-03-03 00:00:00

abstract：:The role of interleukin-8 (IL-8) and related CXC chemokines has been demonstrated in many human diseases. However, more profound studies, e.g., by blocking the effect of these inflammatory mediators, request animal models and hence the identification of all human counterparts for commonly used laboratory animals. In t...

journal_title：Biochemistry

authors： Wuyts A,Schutyser E,Menten P,Struyf S,D'Haese A,Bult H,Opdenakker G,Proost P,Van Damme J

更新日期：2000-11-28 00:00:00

abstract：:Recently, we described the formation of a complex between liposomes and the unfolded protein rhodanese (thiosulfate:cyanide sulfurtransferase, EC 2.8.1.1), which could be liberated and efficiently reactivated after treatment of the complex with detergents [Zardeneta, G., & Horowitz, P. M. (1992) Eur. J. Biochem. 210, ...

journal_title：Biochemistry

authors： Zardeneta G,Horowitz PM

更新日期：1993-12-21 00:00:00

abstract：:PLK1 (polo-like kinase 1) is a key mitotic kinase and a therapeutic target in the treatment of proliferative diseases. Here we investigate the relative substrate specificity and pharmacological relatedness of PLK1, -2, -3, and -4 that together comprise a conserved family of Ser/Thr kinases (PLK family). We report cons...

journal_title：Biochemistry

authors： Johnson EF,Stewart KD,Woods KW,Giranda VL,Luo Y

更新日期：2007-08-21 00:00:00

abstract：:The carboxy-terminal domain of the alpha subunit of Escherichia coli RNA polymerase, which is connected with the core part of RNA polymerase through a long flexible linker, plays decisive roles in transcription activation by directly interacting with a large number of transcription factors and upstream (UP) element DN...

journal_title：Biochemistry

authors： Fujita N,Endo S,Ishihama A

更新日期：2000-05-23 00:00:00

abstract：:Understanding the process of amyloidogenesis is important for the future treatment of misfolding-based diseases, such as Alzheimer's, spongiform encephalopathies, and other important disorders affecting humans. In this work, we have used one of the best-characterized models for folding and misfolding, the activation d...

journal_title：Biochemistry

authors： Cerdà-Costa N,De la Arada I,Avilés FX,Arrondo JL,Villegas S

更新日期：2009-11-10 00:00:00

abstract：:Alkalimetric, spectrophotometric, NMR, and resonance Raman titrations are reported for the sulfonamide Neoprontosil in aqueous solution. An assignment of the magnetic resonance peaks for each of the Neoprontosil protons has been made. Neoprontosil is shown to have two "coupled" iity of the microscopic pKs for these tw...

journal_title：Biochemistry

authors： Carey PR,King RW

更新日期：1979-06-26 00:00:00

abstract：:Despite significant progress in the past decade, questions still remain about the complete structural, dynamic, and thermodynamic effect of the cis-syn cyclobutane pyrimidine dimer lesion (hereafter called the thymine dimer) on double-stranded genomic DNA. We examined a 19-mer oligodeoxynucleotide duplex containing a ...

journal_title：Biochemistry

authors： Rumora AE,Kolodziejczak KM,Malhowski Wagner A,Núñez ME

更新日期：2008-12-09 00:00:00

abstract：:Translesion DNA synthesis represents the ability of a DNA polymerase to misinsert a nucleotide opposite a DNA lesion. Previous kinetic studies of the bacteriophage T4 DNA polymerase using a series of non-natural nucleotides suggest that pi-electron density of the incoming nucleotide substantially contributes to the ef...

journal_title：Biochemistry

authors： Zhang X,Donnelly A,Lee I,Berdis AJ

更新日期：2006-11-07 00:00:00

abstract：:The targeting of the insulin-responsive glucose transporter, GLUT-4, to an intracellular compartment in adipocytes and muscle is one of the key features responsible for the unique insulin sensitivity of this transporter. Through expression of epitope-tagged GLUT-4 mutants in 3T3-L1 adipocytes, two motifs have been ide...

journal_title：Biochemistry

authors： Melvin DR,Marsh BJ,Walmsley AR,James DE,Gould GW

更新日期：1999-02-02 00:00:00

abstract：:Nogo-66 receptor (NgR1) is a leucine-rich repeat (LRR) protein that forms part of a signaling complex modulating axon regeneration. Previous studies have shown that the entire LRR region of NgR1, including the C-terminal cap of the LRR, LRRCT, is needed for ligand binding, and that the adjacent C-terminal region (CT s...

journal_title：Biochemistry

authors： Wen D,Wildes CP,Silvian L,Walus L,Mi S,Lee DH,Meier W,Pepinsky RB

更新日期：2005-12-20 00:00:00

abstract：:Mutation of Gly90, Glu113, Ala292, and Lys296 in the visual pigment rhodopsin constitutively activates the protein for activation of the G protein transducin. Three of these mutations have been shown to cause two different human diseases. Mutation of Gly90 and Ala292 results in complete night blindness, and mutation o...

journal_title：Biochemistry

authors： Rim J,Oprian DD

更新日期：1995-09-19 00:00:00

abstract：:Our recent demonstration that DNA polymerase X (Pol X), the DNA repair polymerase encoded by the African swine fever virus (ASFV), is extremely error prone during single-nucleotide gap filling led us to hypothesize that it might contribute to genetic variability in ASFV. For the infidelity of Pol X to be relevant, how...

journal_title：Biochemistry

authors： Lamarche BJ,Showalter AK,Tsai MD

更新日期：2005-06-14 00:00:00