Rational attempts to optimize non-natural nucleotides for selective incorporation opposite an abasic site.

abstract：:Factor V(a) (FV(a)) is a cofactor for the serine protease factor X(a) that activates prothrombin to thrombin in the presence of Ca(2+) and a membrane surface. FV(a) is a heterodimer composed of one heavy chain (A1 and A2 domains) and one light chain (A3, C1, and C2 domains). We use fluorescence, circular dichroism, an...

journal_title：Biochemistry

authors： Srivastava A,Quinn-Allen MA,Kim SW,Kane WH,Lentz BR

更新日期：2001-07-27 00:00:00

abstract：:Rhodopsin kinase phosphorylates serine- and threonine-containing peptides from bovine rhodopsin's carboxyl-terminal sequence. Km's for the peptides decrease as the length of the peptide is increased over the range 12-31 amino acids, reaching 1.7 mM for peptide 318-348 from the rhodopsin sequence. The Km for phosphoryl...

journal_title：Biochemistry

authors： Palczewski K,Arendt A,McDowell JH,Hargrave PA

更新日期：1989-10-31 00:00:00

abstract：:We prepared a series of cryptogein mutants, an elicitor from Phytophthora cryptogea, with altered abilities to bind sterols and fatty acids. The induction of the early events, i.e., synthesis of active oxygen species and pH changes, in suspension tobacco cells by these mutated proteins was proportional to their abilit...

journal_title：Biochemistry

authors： Lochman J,Kasparovsky T,Damborsky J,Osman H,Marais A,Chaloupkova R,Ponchet M,Blein JP,Mikes V

更新日期：2005-05-03 00:00:00

abstract：:A detailed interpretation of the grossly distorted ultraviolet absorption and circular dichroism spectra of the intact red blood cell is given, including an evaluation of the effects of protein conformation, detector geometry, cell hemoglobin content, and refractive index on the calculated cell spectra. The origins of...

journal_title：Biochemistry

authors： Gitter-Amir A,Schneider AS,Rosenheck K

更新日期：1976-07-13 00:00:00

abstract：:Most duplex DNAs that are in the "B" conformation are not immunogenic. One important exception is poly(dG) X poly(dC), which produces a good immune response even though, by many criteria, it adopts a conventional right-handed helix. In order to investigate what features are being recognized, monoclonal antibodies were...

journal_title：Biochemistry

authors： Lee JS,Woodsworth ML,Latimer LJ

更新日期：1984-07-03 00:00:00

abstract：:The role of interleukin-8 (IL-8) and related CXC chemokines has been demonstrated in many human diseases. However, more profound studies, e.g., by blocking the effect of these inflammatory mediators, request animal models and hence the identification of all human counterparts for commonly used laboratory animals. In t...

journal_title：Biochemistry

authors： Wuyts A,Schutyser E,Menten P,Struyf S,D'Haese A,Bult H,Opdenakker G,Proost P,Van Damme J

更新日期：2000-11-28 00:00:00

abstract：:The nitrile stretching vibration is increasingly used as a sensitive infrared probe of local protein environments. However, site-specific incorporation of a nitrile moiety into proteins is difficult. Here we show that various aromatic nitriles can be easily incorporated into peptides and proteins via either thiol alky...

journal_title：Biochemistry

authors： Jo H,Culik RM,Korendovych IV,Degrado WF,Gai F

更新日期：2010-12-14 00:00:00

abstract：:Thermal and chemical modification of protein structures is known to affect their interfacial activity. We have looked in detail at the effect of heating on the structure and subsequently the surface properties of LTP1, a nonspecific lipid transfer protein from barley, both in the presence and in the absence of its lip...

journal_title：Biochemistry

authors： Clare Mills EN,Gao C,Wilde PJ,Rigby NM,Wijesinha-Bettoni R,Johnson VE,Smith LJ,Mackie AR

更新日期：2009-12-29 00:00:00

abstract：:The effects of site-directed mutagenesis were used to explore the role of residues in M4 on the apparent Ki of a selective, K+-competitive inhibitor of the gastric H+,K+ ATPase, SCH28080. A double transfection expression system is described, utilizing HEK293 cells and separate plasmids encoding the alpha and beta subu...

journal_title：Biochemistry

authors： Munson KB,Lambrecht N,Sachs G

更新日期：2000-03-21 00:00:00

abstract：:Recent crystal structures of the small ribosomal subunit have made it possible to examine the detailed energetics of codon recognition on the ribosome by computational methods. The binding of cognate and near-cognate anticodon stem loops to the ribosome decoding center, with mRNA containing the Phe UUU and UUC codons,...

journal_title：Biochemistry

authors： Almlöf M,Andér M,Aqvist J

更新日期：2007-01-09 00:00:00

abstract：:Oligopeptides and dipeptides are transported into Saccharomyces cerevisiae by a carrier-mediated system. In the dark, leucyl-p-nitroanilide (Leu-p-NA) and leucyl-leucyl-4-azido-2-nitrophenylalanine [Leu-Leu-Phe-(4N3,2NO2)] are competitive inhibitors of peptide transport by S. cerevisiae cells. The photolysis of yeast ...

journal_title：Biochemistry

authors： Becker JM,Dunsmore KP,Steinfeld AS,Naider F

更新日期：1982-11-09 00:00:00

abstract：:Human alpha-lactalbumin (alpha-LA), a 123-residue calcium-binding protein, has been studied using (15)N NMR relaxation methods in order to characterize backbone dynamics of the native state at the level of individual residues. Relaxation data were collected at three magnetic field strengths and analyzed using the mode...

journal_title：Biochemistry

authors： Bruylants G,Redfield C

更新日期：2009-05-19 00:00:00

abstract：:The catalysis of the chemical exchange between acetaldehyde and its hydrate by bovine carbonic anhydrase B was investigated by the NMR line broadening and saturation transfer techniques. While both chemical exchange and binding to the enzyme had an effect on the line broadening of acetaldehyde and its hydrate, the sat...

journal_title：Biochemistry

authors： Cheshnovsky D,Navon G

更新日期：1980-04-29 00:00:00

abstract：:Neuronal PAS protein 2 (NPAS2), a heme-binding transcriptional regulatory factor, is involved in circadian rhythms. Period homologue (Per) is another important transcriptional regulatory factor that binds to cryptochrome (Cry). The resultant Per/Cry heterodimer interacts with the NPAS2/BMAL1 heterodimer to inhibit the...

journal_title：Biochemistry

authors： Kitanishi K,Igarashi J,Hayasaka K,Hikage N,Saiful I,Yamauchi S,Uchida T,Ishimori K,Shimizu T

更新日期：2008-06-10 00:00:00

abstract：:Probing and interrogating protein interactions that involve acyl carrier proteins (ACP's) in fatty acid synthases and polyketide synthases are critical to understanding the molecular basis for the programmed assembly of complex natural products. Here, we have used unnatural amino acid mutagenesis to site specifically ...

journal_title：Biochemistry

authors： Ye Z,Williams GJ

更新日期：2014-12-09 00:00:00

abstract：:The crystal structure of the complex between the quinoprotein methylamine dehydrogenase (MADH) and the type I blue copper protein amicyanin, both from Paracoccus denitrificans, has been determined at 2.5-A resolution using molecular replacement. The search model was MADH from Thiobacillus versutus. The amicyanin could...

journal_title：Biochemistry

authors： Chen L,Durley R,Poliks BJ,Hamada K,Chen Z,Mathews FS,Davidson VL,Satow Y,Huizinga E,Vellieux FM

更新日期：1992-06-02 00:00:00

abstract：:We report on the determination of the solution structure of two sequence-related oligonucleotides, d(GTACGTAC)2 and d(CATCGATG)2. Results have been obtained by using a combined approach of (a) two-dimensional NMR, including proton and phosphorus experiments, (b) restrained molecular mechanics performed with sugar phas...

journal_title：Biochemistry

authors： Lefebvre A,Mauffret O,Hartmann B,Lescot E,Fermandjian S

更新日期：1995-09-19 00:00:00

abstract：:Yeast mitochondrial NAD+-specific isocitrate dehydrogenase (IDH) has previously been shown to bind specifically to 5'-untranslated regions of yeast mitochondrial mRNAs, and transcripts containing these regions have been found to allosterically inhibit activity of the enzyme. This inhibition is relieved by AMP, an allo...

journal_title：Biochemistry

authors： Anderson SL,Schirf V,McAlister-Henn L

更新日期：2002-06-04 00:00:00

abstract：:AMSH, a deubiquitinating enzyme (DUB) with exquisite specificity for Lys63-linked polyubiquitin chains, is an endosome-associated DUB that regulates sorting of activated cell-surface signaling receptors to the lysosome, a process mediated by the members of the endosomal sorting complexes required for transport (ESCRT)...

journal_title：Biochemistry

authors： Davies CW,Paul LN,Das C

更新日期：2013-11-05 00:00:00

abstract：:The effects of Ca2+, Mg2+, and troponin I (TnI) inhibitory peptide (Ip) binding on the spectral properties of a Phe-154 to Trp mutant (F154W) of chicken recombinant troponin C (rTnC) have been examined. Residue 154 is positioned in the final flanking helix H of metal binding site IV. Since there are no other Tyr or Tr...

journal_title：Biochemistry

authors： Chandra M,McCubbin WD,Oikawa K,Kay CM,Smillie LB

更新日期：1994-03-15 00:00:00

abstract：:Inwardly rectifying potassium channels are important in cellular repolarization of many excitable tissues. Amino acid sequence alignment of different mammalian inward rectifier K(+) channels revealed two absolutely conserved cysteine residues in the putative extracellular face, suggesting a possible disulfide bond. Re...

journal_title：Biochemistry

authors： Cho HC,Tsushima RG,Nguyen TT,Guy HR,Backx PH

更新日期：2000-04-25 00:00:00

abstract：:Fusion of a prokaryotic promoter to a yeast tRNA gene provides a means for uncoupling analyses of mutations affecting splicing from requirements for transcription and other processing steps. For this purpose, a phage lambda promoter was fused to the Saccharomyces cerevisiae tRNATyr(SUP3a) coding sequence. This fusion ...

journal_title：Biochemistry

authors： Shapero MH,Greer CL

更新日期：1991-07-02 00:00:00

abstract：:Actin, an abundant cytosolic protein in eukaryotic cells, is dependent on the interaction with the chaperonin tail-less complex polypeptide 1 ring complex (TRiC) to fold to the native state. The prokaryotic chaperonin GroEL also binds non-native beta-actin, but is unable to guide beta-actin toward the native state. In...

journal_title：Biochemistry

authors： Villebeck L,Moparthi SB,Lindgren M,Hammarström P,Jonsson BH

更新日期：2007-11-06 00:00:00

abstract：:The RecB and RecC subunits of the RecBCD enzyme from Escherichia coli were purified from cells containing plasmids overproducing these proteins [Boehmer, P.E., & Emmerson, P.T. (1991) Gene 102, 1-6]. RecB hydrolyzes ATP in the presence of either single- or double-stranded DNA. RecC stimulates ATP hydrolysis by RecB, p...

journal_title：Biochemistry

authors： Korangy F,Julin DA

更新日期：1993-05-11 00:00:00

abstract：:1,3-15N-Labeled uracil was synthesized chemically and used to prepare labeled Escherichia coli tRNA(Val) biosynthetically. 500-MHz measurements of 15N and proton chemical shift were obtained, for all uridine and uridine-related bases, by heteronuclear multiple-quantum coherence spectroscopy. All the uracil NH group re...

journal_title：Biochemistry

authors： Choi BS,Redfield AG

更新日期：1992-12-29 00:00:00

abstract：:Binding of Ca(2+)-activated calmodulin (Ca(2+)-CaM) to neuronal nitric-oxide synthase (nNOS) increases the rate of 2,6-dichloroindolphenol (DCIP) reduction 2-3-fold and that of cytochrome c(3+) 10-20-fold. Parallel initial velocity patterns indicated that both substrates were reduced via two-half reactions in a ping-p...

journal_title：Biochemistry

authors： Wolthers KR,Schimerlik MI

更新日期：2001-04-17 00:00:00

abstract：:Staphylococcus aureus is a leading cause of surgical site infections that results in increased hospital stays due to the development of chronic wounds. Little is known about factors involved in S. aureus' ability to prevent wounds from healing. We discovered a novel secreted protein produced by a surgical site isolate...

journal_title：Biochemistry

authors： Merriman JA,Klingelhutz AJ,Diekema DJ,Leung DY,Schlievert PM

更新日期：2015-08-11 00:00:00

abstract：:Nephrocalcin (NC), a calcium-binding glycoprotein of 14,000 molecular weight as a monomer, is known to inhibit the growth of calcium oxalate monohydrate (COM) crystals in renal tubules. We have isolated NC from bovine kidney tissue and purified into four isoforms, fractions A-D. NC-A and NC-B strongly inhibit the grow...

journal_title：Biochemistry

authors： Mustafi D,Nakagawa Y

更新日期：1996-11-26 00:00:00

abstract：:Peptide NH resonances in the 250 MHZ 1H nuclear magnetic resonance (NMR) spectrum of oxytocin in H2O were assigned to specific amino acid residues by the "underwater decoupling" technique (i.e., decoupling from corresponding CalphaH resonances, which are buried beneath the intense water peak). These experiments confir...

journal_title：Biochemistry

authors： Glickson JD,Rowan R,Pitner TP,Dadok J,Bothner-By AA,Walter R

更新日期：1976-03-09 00:00:00

abstract：:A nucleoside triphosphate binding site on calf thymus RNA polymerase II was identified by using photoaffinity analogues of adenosine 5'-triphosphate and guanosine 5'-triphosphate. Both radiolabeled 8-azidoadenosine 5'-triphosphate (8-N3ATP) and radiolabeled 8-azidoguanosine 5'-triphosphate (8-N3GTP) bound to a single ...

journal_title：Biochemistry

authors： Freund E,McGuire PM

更新日期：1986-01-14 00:00:00