Novel Staphylococcus aureus Secreted Protein Alters Keratinocyte Proliferation and Elicits a Proinflammatory Response In Vitro and In Vivo.

abstract：:In animals, the post-translational hydroxylation of hypoxia inducible factor (HIF) is a central mechanism for regulating gene expression in an oxygen-dependent manner. The oxygenase-catalyzed trans-4-prolyl hydroxylation of HIF-alpha increases its affinity for the von Hippel Lindau protein elongin B/C (VCB) ubiquitin ...

journal_title：Biochemistry

authors： Illingworth CJ,Loenarz C,Schofield CJ,Domene C

更新日期：2010-08-17 00:00:00

abstract：:The coordination complex tris(4,7-diphenyl-1,10-phenanthroline)rhodium(III) [Rh(DIP)3(3+)], which promotes RNA cleavage upon photoactivation, has been shown to target specifically guanine-uracil (G-U) mismatches in double-helical regions of folded RNAs. Photoactivated cleavage by Rh(DIP)3(3+) has been examined on a se...

journal_title：Biochemistry

authors： Chow CS,Barton JK

更新日期：1992-06-23 00:00:00

abstract：:The multifunctional proline utilization A (PutA) flavoenzyme from Escherichia coli catalyzes the oxidation of proline to glutamate in two reaction steps using separate proline dehydrogenase (PRODH) and Δ(1)-pyrroline-5-carboxylate (P5C) dehydrogenase domains. Here, the kinetic mechanism of PRODH in PutA is studied by ...

journal_title：Biochemistry

authors： Moxley MA,Becker DF

更新日期：2012-01-10 00:00:00

abstract：:The cyclic antimicrobial lipopeptide daptomycin is now frequently used as a first-line therapy in serious infections caused by multidrug-resistant Enterococcus faecium. Resistance to daptomycin in E. faecium is mediated by activation of the LiaFSR membrane stress response pathway. Deletion of liaR, encoding the respon...

journal_title：Biochemistry

authors： Davlieva M,Wu C,Zhou Y,Arias CA,Shamoo Y

更新日期：2018-12-11 00:00:00

abstract：:All retroviruses package two copies of their genomes during virus assembly, both of which are required for strand transfer-mediated recombination during reverse transcription. Genome packaging is mediated by interactions between the nucleocapsid (NC) domains of assembling Gag polyproteins and an RNA packaging signal, ...

journal_title：Biochemistry

authors： Dey A,York D,Smalls-Mantey A,Summers MF

更新日期：2005-03-15 00:00:00

abstract：:We extended the technique of frequency-domain fluorometry to an upper frequency limit of 2000 MHz. This was accomplished by using the harmonic content of a laser pulse train (3.76 MHz, 5 ps) from a synchronously pumped and cavity-dumped dye laser. We used a microchannel plate photomultiplier as the detector to obtain ...

journal_title：Biochemistry

authors： Lakowicz JR,Laczko G,Gryczynski I

更新日期：1987-01-13 00:00:00

abstract：:p-Hydroxyphenylpyruvate dioxygenase (HPD) plays a key role in the normal catabolism of tyrosine. An Fe2+/oxygen-dependent enzyme, it converts p-hydroxyphenylpyruvate into homogentisate and is part of the superfamily of alpha-ketoglutarate-dependent enzymes that couples oxidative decarboxylation of an alpha-ketoacid co...

journal_title：Biochemistry

authors： Gunsior M,Ravel J,Challis GL,Townsend CA

更新日期：2004-01-27 00:00:00

abstract：:Carbamoyl phosphate synthetase from E. coli catalyzes the synthesis of carbamoyl phosphate through a series of four reactions occurring at three active sites connected by a molecular tunnel of 100 A. To understand the mechanism for coordination and synchronization among the active sites, the pre-steady-state time cour...

journal_title：Biochemistry

authors： Miles BW,Raushel FM

更新日期：2000-05-02 00:00:00

abstract：:Illumination of envelope vesicles prepared from Halobacterium halobium cells causes translocation of protons from inside to outside, due to the light-induced cycling of bacteriorhodopsin. This process results in a pH gradient across the membranes, an electrical potential, and the movements of K+ and Na+. The electrica...

journal_title：Biochemistry

authors： Renthal R,Lanyi JK

更新日期：1976-05-18 00:00:00

abstract：:Myosin belongs to the family of motor proteins. Its interaction with actin coupled with hydrolysis of ATP is the molecular basis of muscle contraction. The head segment of myosin, called subfragment 1 (S1), contains the distinct binding sites for ATP and actin and responsible for the ATPase activity. The rate-limiting...

journal_title：Biochemistry

authors： Peyser YM,Ben-Hur M,Werber MM,Muhlrad A

更新日期：1996-04-09 00:00:00

abstract：:R67 dihydrofolate reductase (DHFR) is a novel homotetrameric protein that possesses 222 symmetry and a single, voluminous active site pore. This symmetry poses numerous limitations on catalysis; for example, two dihydrofolate (DHF) molecules or two NADPH molecules, or one substrate plus one cofactor can bind. Only the...

journal_title：Biochemistry

authors： Chopra S,Lynch R,Kim SH,Jackson M,Howell EE

更新日期：2006-05-30 00:00:00

abstract：:The Y70F mutant of aspartate aminotransferase has reduced affinity for coenzymes compared to the wild type. The equilibrium dissociation constants for pyridoxamine phosphate (PMP) holoenzymes, KPMPdiss, were determined from the association and dissociation rate constants to be 1.3 nM and 30 nM for the wild type and mu...

journal_title：Biochemistry

authors： Toney MD,Kirsch JF

更新日期：1991-07-30 00:00:00

abstract：:The electron paramagnetic resonance (EPR) spectra of spin-labeled DNA duplexes, both bound to DEAE-Sephadex and free in solution, have been analyzed. The nitroxide spin-labels are covalently linked to a deoxyuridine residue using either a monoacetylene or diacetylene tether. This difference in tether length produces a...

journal_title：Biochemistry

authors： Hustedt EJ,Kirchner JJ,Spaltenstein A,Hopkins PB,Robinson BH

更新日期：1995-04-04 00:00:00

abstract：:Microdomains known as "rafts" have been isolated from many cell types as detergent-resistant membranes (DRMs) and are enriched in sphingolipids and cholesterol. However, there has been considerable controversy over whether such domains are found in native membranes or are artificially generated by the purification pro...

journal_title：Biochemistry

authors： Luria A,Vegelyte-Avery V,Stith B,Tsvetkova NM,Wolkers WF,Crowe JH,Tablin F,Nuccitelli R

更新日期：2002-11-05 00:00:00

abstract：:Green sulfur bacteria possess two light-harvesting antenna systems, the chlorosome and the Fenna-Matthews-Olson (FMO) protein. In addition to self-aggregated bacteriochlorophyll (BChl) c, chlorosomes of Chlorobium tepidum contain a small amount of BChl a (ratio 100:1). The chlorosomal BChl a is associated with CsmA, a...

journal_title：Biochemistry

authors： Pedersen MØ,Pham L,Steensgaard DB,Miller M

更新日期：2008-02-05 00:00:00

abstract：:If cytochrome c oxidase is subjected to pressure during the aerobic steady state, large spectral changes are apparent. These seem to be associated with the inhibition of electron transport within the oxidase. The volume change for the transition is about 80 mL/mol. When the oxidase in the aerobic steady state, with po...

journal_title：Biochemistry

authors： Kornblatt JA,Hui Bon Hoa G,Heremans K

更新日期：1988-07-12 00:00:00

abstract：:The phosphoryl-binding elements in the GDP-binding domain of elongation factor Tu were studied by heteronuclear proton observe methods. Five proton resonances were found below 10.5 ppm. Two of these were assigned to the amide groups of Lys 24 and Gly 83. These are conserved residues in each of the consensus sequences....

journal_title：Biochemistry

authors： Lowry DF,Cool RH,Redfield AG,Parmeggiani A

更新日期：1991-11-12 00:00:00

abstract：:Hydrophobic interaction of 8-anilino-1-naphthalene sulfonic acid (ANS) with proteins is one of the widely used methods for characterizing/detecting partially folded states of proteins. We have carried out a systematic investigation on the effect of ANS, a charged hydrophobic fluorescent dye, on structural properties o...

journal_title：Biochemistry

authors： Ali V,Prakash K,Kulkarni S,Ahmad A,Madhusudan KP,Bhakuni V

更新日期：1999-10-12 00:00:00

abstract：:d-2-Hydroxyglutarate dehydrogenase from Pseudomonas aeruginosa PAO1 (PaD2HGDH) catalyzes the oxidation of d-2-hydroxyglutarate to 2-ketoglutarate, which is a necessary step in the serine biosynthetic pathway. The dependence of P. aeruginosa on PaD2HGDH makes the enzyme a potential therapeutic target against P. aerugin...

journal_title：Biochemistry

authors： Quaye JA,Gadda G

更新日期：2020-12-29 00:00:00

abstract：:The Gbeta and Ggamma subunit of the heterotrimeric G proteins form a functional dimer that is stable once assembled in vivo or in vitro. The requirements, mechanism, and specificity of dimer formation are still incompletely understood, but represent important biochemical processes involved in the specificity of cellul...

journal_title：Biochemistry

authors： Dingus J,Wells CA,Campbell L,Cleator JH,Robinson K,Hildebrandt JD

更新日期：2005-09-06 00:00:00

abstract：:The Rieske protein from Thermus thermophilus (TtRp) and a truncated version of the protein (truncTtRp), produced to achieve a low-pH crystallization condition, have been characterized using UV-visible and circular dichroism spectroscopies. TtRp and truncTtRp undergo a change in the UV-visible spectra with increasing p...

journal_title：Biochemistry

authors： Konkle ME,Muellner SK,Schwander AL,Dicus MM,Pokhrel R,Britt RD,Taylor AB,Hunsicker-Wang LM

更新日期：2009-10-20 00:00:00

abstract：:The structure of a mutant Escherichia coli thioredoxin with a glutamic acid substituted for a conserved lysine at position 36 adjacent to the active site has been solved using molecular replacement and refined at 2.0-A resolution to a crystallographic residual of 19.9%. The mutant was crystallized in an orthorhombic s...

journal_title：Biochemistry

authors： Nikkola M,Gleason FK,Fuchs JA,Eklund H

更新日期：1993-05-18 00:00:00

abstract：:Herpesvirus saimiri codes for a tyrosine kinase interacting protein (Tip) that interacts with both the SH3 domain and the kinase domain of the T-cell-specific tyrosine kinase Lck via two separate motifs. The activation of Lck by Tip is considered as a key event in the transformation of human T-lymphocytes during herpe...

journal_title：Biochemistry

authors： Schweimer K,Hoffmann S,Bauer F,Friedrich U,Kardinal C,Feller SM,Biesinger B,Sticht H

更新日期：2002-04-23 00:00:00

abstract：:In this work, we have studied the role of the arginine finger region in determining the specificity of the GTPase activating proteins (GAPs) Saccharomyces cerevisiae Ira2p and human p120-GAP toward yeast Ras2p and human Ha-Ras p21. It is known that p120-GAP can enhance both Ras2p and Ha-Ras GTPase activities, whereas ...

journal_title：Biochemistry

authors： te Biesebeke R,Krab IM,Parmeggiani A

更新日期：2001-06-26 00:00:00

abstract：:Cibacron Blue F3GA, the polycyclic blue chromophore of Blue Dextran 2000, inhibits the catalytic subunit of bovine brain protein kinase. The rate of inactivation exhibits a hyperbolic dependence on the dye concentration. This suggests that an enzyme-dye complex forms prior to inactivation. Protein and peptide substrat...

journal_title：Biochemistry

authors： Witt JJ,Roskoski R Jr

更新日期：1980-01-08 00:00:00

abstract：:Various 6-alkylubiquinone or 6-(omega-haloalkyl)ubiquinone derivatives were synthesized through a radical coupling reaction between alkanoyl or omega-haloalkanoyl peroxides and ubiquinone 0. The latter was synthesized from 2-methoxy-4-methylphenol via nitration, methylation, reduction, and oxidation by modifications o...

journal_title：Biochemistry

authors： Yu CA,Yu L

更新日期：1982-08-17 00:00:00

abstract：:There is considerable evidence that mammalian beta-tubulin is phosphorylated. Specifically, of the seven beta isotypes, the phosphorylated one is beta III, the isotype found almost entirely in neurons. The phosphate is added at a serine and perhaps a tyrosine near the C-terminus. All the evidence to date has been gath...

journal_title：Biochemistry

authors： Khan IA,Ludueña RF

更新日期：1996-03-26 00:00:00

abstract：:The role of acetylation on the conformation and association state of the inner core histone octamer isolated from HeLa cells was examined. Preparation of suitable quantities of pure acetylated and control inner core histones from HeLa cells required the development of a new preparative procedure. The results from size...

journal_title：Biochemistry

authors： Prevelige PE Jr,Fasman GD

更新日期：1987-05-19 00:00:00

abstract：:Expression of the PSR132 protein from Dianthus caryophyllus (carnation, clover pink) is induced in response to ethylene production associated with petal senescence, and thus the protein is named petal death protein (PDP). Recent work has established that despite the annotation of PDP in sequence databases as carboxyph...

journal_title：Biochemistry

authors： Teplyakov A,Liu S,Lu Z,Howard A,Dunaway-Mariano D,Herzberg O

更新日期：2005-12-20 00:00:00

abstract：:In the present study we establish that specific members of the Sp1 family of transcription factors (Sp1 and Sp3) bind to all six GC-rich motifs (elements 1-6) present in the proximal promoter of the human cell cycle inhibitor p21(WAF-1/Cip1) gene. Competition analysis showed that Sp1 and Sp3 bound with high affinity t...

journal_title：Biochemistry

authors： Koutsodontis G,Moustakas A,Kardassis D

更新日期：2002-10-22 00:00:00