Abstract：
:Green sulfur bacteria possess two light-harvesting antenna systems, the chlorosome and the Fenna-Matthews-Olson (FMO) protein. In addition to self-aggregated bacteriochlorophyll (BChl) c, chlorosomes of Chlorobium tepidum contain a small amount of BChl a (ratio 100:1). The chlorosomal BChl a is associated with CsmA, a 6.2 kDa protein that accounts for more than 50% of the protein content of chlorosomes. This CsmA-BChl a complex is located in the chlorosome baseplate with the hydrophilic C-terminal part of CsmA in contact with the FMO protein. CsmA was purified from Chl. tepidum. Isolated chlorosomes were lyophilized and extracted with chloroform/methanol (1:1, v/v). The extract was further purified using gel filtration and reverse-phase HPLC and the purity of the preparation confirmed by SDS-PAGE. Mass spectrometric analysis showed an m/z of 6154.8, in agreement with the calculated mass of the csmA gene product after C-terminal processing. CD spectroscopy of the isolated protein showed that the main structural motif was an alpha-helix. We have reconstituted the isolated CsmA protein with BChl a in micelles of n-octyl beta-d-glucopyranoside. The resulting preparation reproduced the spectral characteristics of the CsmA-BChl a complex present in the chlorosome baseplate.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Pedersen MØ,Pham L,Steensgaard DB,Miller Mdoi
10.1021/bi701616rsubject
Has Abstractpub_date
2008-02-05 00:00:00pages
1435-41issue
5eissn
0006-2960issn
1520-4995journal_volume
47pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The kinetics of the slow folding and unfolding reactions of barstar, a bacterial ribonuclease inhibitor protein, have been studied at 23(+/-1) degrees C, pH 8, by the use of tryptophan fluorescence, far-UV circular dichroism (CD), near-UV CD, and transient mixing (1)H nuclear magnetic resonance (NMR) spectroscopic mea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990285w
更新日期：1999-07-13 00:00:00
abstract：:We have determined the nucleotide sequence and the derived amino acid sequence of the phosphoprotein-encoding region of the chicken vitellogenin III gene. The sequence of this minor vitellogenin could be aligned with exon 22 up to exon 27 of the previously sequenced major vitellogenin II gene (van het Schip et al., 19...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00432a034
更新日期：1989-03-21 00:00:00
abstract：:The importance of DNA polymerase-DNA minor groove interactions on translesion synthesis (TLS) was examined in vitro using variants of exonuclease-deficient Klenow polymerase and site-specifically modified DNA oligonucleotides. Polymerase variant R668A lacks primer strand interactions, while variant Q849A lacks templat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi047591g
更新日期：2005-05-10 00:00:00
abstract：:Substrates homoprotocatechuate (HPCA) and O(2) bind to the Fe(II) of homoprotocatechuate 2,3-dioxygenase (FeHPCD) in adjacent coordination sites. Transfer of an electron(s) from HPCA to O(2) via the iron is proposed to activate the substrates for reaction with each other to initiate aromatic ring cleavage. Here, rapid...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201436n
更新日期：2011-11-29 00:00:00
abstract：:We have investigated the interaction of the bisbenzimidazole derivative Hoechst 33258 with the self-complementary dodecadeoxynucleotide duplex d(GTGGAATTCCAC)2 using one-dimensional (1D) and two-dimensional (2D) proton nuclear magnetic resonance (1H NMR) spectroscopy. To monitor the extent of complex formation, we use...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00112a004
更新日期：1991-12-03 00:00:00
abstract：:The mechanisms by which multisubunit histone acetyltransferase (HAT) complexes recognize and perform efficient acetylation on nucleosome substrates are largely unknown. Here, we use a variety of biochemical approaches and compare histone-based substrates of increasing complexity to determine the critical components of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi602366n
更新日期：2007-02-27 00:00:00
abstract：:The enthalpies of transfer of hen egg white lysozyme from water to aqueous solutions of 1-propanol were determined by isothermal flow calorimetry at 10, 17, 25, and 40 degrees C in 0.04 M, pH 2 glycine buffer. Alcohol concentrations up to 3.4 M were employed. Four regions in the dependence of the enthalpy of transfer ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00514a016
更新日期：1981-05-26 00:00:00
abstract：:The interaction of the Escherichia coli single-stranded DNA binding protein (SSB) with oligo- and poly-nucleotides has been studied by 270-MHz 1H NMR spectroscopy and fast kinetic techniques. d(pT)8 and poly(dT) were used to study noncooperative and cooperative binding, respectively. The H6, H1', and CH3 resonances of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00320a036
更新日期：1984-12-04 00:00:00
abstract：:Understanding the structural basis for defects in protein function that underlie protein-based genetic diseases is the fundamental requirement for development of therapies. This situation is epitomized by the cystic fibrosis transmembrane conductance regulator (CFTR)-the gene product known to be defective in CF patien...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi702209s
更新日期：2008-02-12 00:00:00
abstract：:In order to test the proposal [Stehle, T., Claiborne, A., & Schulz, G. E. (1993) Eur. J. Biochem. 211, 221-226] that the active-site His10 of NADH peroxidase functions as an essential acid-base catalyst, we have analyzed mutants in which this residue has been replaced by Gln or Ala. The k(cat) values for both H10Q and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952347y
更新日期：1996-02-20 00:00:00
abstract：:The hammerhead ribozyme undergoes a well-defined two-stage conformational folding process, induced by the binding of magnesium ions. In this study, we have used isothermal titration calorimetry to analyze the thermodynamics of magnesium binding and magnesium ion-induced folding of the ribozyme. Binding to the natural ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002231o
更新日期：2001-02-06 00:00:00
abstract：:Phosphorus-31 magic angle sample spinning NMR spectra have been employed to investigate the structure and composition of the mineral deposits in chicken bone. Three different pulse sequences, Bloch decay, cross-polarization, and dipolar suppression, were employed to obtain spectra from bone specimens of varying age. T...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00320a033
更新日期：1984-12-04 00:00:00
abstract：:The 70-kDa heat-shock protein (Hsp70) has been cloned and sequenced from bovine cardiac muscle. On the basis of sequence features, the gene corresponds to the cytoplasmic form of Hsp70. This cardiac Hsp70 cDNA clone has an open reading frame of 1926 bp coding for 641 amino acids and a predicted molecular mass of 70.25...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049036k
更新日期：2004-10-26 00:00:00
abstract：:Ultraviolet absorption techniques were used to study the thermodynamics of duplex formation for a DNA decamer, d(GCGAAAAGCG).d(CGCTTTTCGC), and a series of related duplexes, each of which contains a bulged base centered in the A.T tract. Thermodynamic parameters were obtained from nonlinear least-squares fits of the m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00230a031
更新日期：1991-04-23 00:00:00
abstract：:Nitric oxide synthases (NOSs) are proposed to generate NO and citrulline from L-arginine in two steps: initial N-hydroxylation to generate Nomega-hydroxyarginine (NOHA) followed by a three-electron oxidation of the hydroxylated nitrogen to form products. Both steps consume NADPH and may involve heme iron-based activat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971414g
更新日期：1997-09-09 00:00:00
abstract：:Pyrophosphate-dependent 6-phosphofructo-1-kinase (PPi-PFK) from Propionibacterium freudenreichii was inactivated by low concentrations of the lysine-specific reagent pyridoxal phosphate (PLP) after sodium borohydride reduction. The substrates fructose 6-phosphate and fructose 1,6-bisphosphate protected against inactiv...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00135a011
更新日期：1992-05-26 00:00:00
abstract：:Escherichia coli 16S RNA from 30S ribosomal subunits was isolated, oxidized at the 3' end, and labeled with the thiosemicarbazide derivatives of fluorescein or eosin. Labeled 16S RNA was reconstituted into 30S subunits. They were almost fully active compared to 30S subunits reconstituted from unlabeled 16S RNA by usin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00521a033
更新日期：1981-09-01 00:00:00
abstract：:Absorbance difference spectra were recorded at 20 degrees C from 30 ns to milliseconds after photolysis of lauryl maltoside suspensions of artificial visual pigments derived from 9-cis isomers of 5-ethylretinal, 8,16-methanoretinal (a 6-s-trans-bicyclic analogue), or 5-demethyl-8-methylretinal. In all three pigments, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011461f
更新日期：2002-02-12 00:00:00
abstract：:Intracellular poliovirus-specific RNA species can be measured directly by electrophoresis of total cytoplasmic nucleic acids through 1% agarose gels, resulting in the separation of single- and double-stranded forms of poliovirus RNA from each other and from HeLa cell 28S ribosomal RNA. Single-stranded RNA molecules di...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00631a027
更新日期：1977-06-14 00:00:00
abstract：:Resonance Raman spectra of the photosynthetic reaction center isolated from the green bacterium Chloroflexus aurantiacus have been obtained with excitation in the near-infrared absorption bands of the special pair (P) and the accessory bacteriochlorophyll (B) using shifted-excitation Raman difference spectroscopy (SER...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00015a044
更新日期：1995-04-18 00:00:00
abstract：:Caldesmon from chicken gizzard muscle has been examined for its ability to interact with caltropin using affinity chromatography and the fluorescent probe acrylodan. The action of caltropin on the inhibitory effect of caldesmon on actomyosin ATPase was also studied. Like calmodulin, caltropin could release the inhibit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00162a031
更新日期：1992-12-01 00:00:00
abstract：:Interaction of affinity chromatographically purified antihistone H3 and antihistone H4 with isolated HeLa core particles, followed by separation of unreacted and reacted particles by sedimentation, demonstrates that every core particle contains these histones. Taken together with our previous data indicating the prese...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00643a033
更新日期：1977-11-29 00:00:00
abstract：:We mutated residue 166, located in the putative Na(+) transport pathway between transmembrane segments 4 and 5 of human Na(+)/glucose cotransporter (hSGLT1), from alanine to cysteine (A166C). A166C was expressed in Xenopus laevis oocytes, and electrophysiological methods were used to assay function. The affinity for N...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011026l
更新日期：2001-10-02 00:00:00
abstract：:Acyl carrier protein (ACP) is the central player in fatty acid (FA) biosynthesis. It covalently binds all FA intermediates and presents them to the enzymes needed for elongation. Bacterial ACP must interact with a large number of proteins, which raises the question of how different acyl-ACPs are recognized and disting...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901713r
更新日期：2010-04-06 00:00:00
abstract：:Attenuated total reflection Fourier transform infrared (ATR-FTIR) difference spectroscopy has been performed on samples of bovine cytochrome c oxidase that have been deposited as a thin film on the surface of a silicon microprism. The technique has several advantages over transmission methods in terms of amount of mat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0109717
更新日期：2002-01-22 00:00:00
abstract：:Many strains of Campylobacter jejuni display modified heptose residues in their capsular polysaccharides (CPS). The precursor heptose was previously shown to be GDP-d-glycero-α-d-manno-heptose, from which a variety of modifications of the sugar moiety have been observed. These modifications include the generation of 6...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00096
更新日期：2020-04-07 00:00:00
abstract：:Protein arginine methyltransferases (PRMTs) catalyze the transfer of methyl groups from S-adenosylmethionine (SAM) to the guanidinium group of arginine residues in a number of important cell signaling proteins. PRMT1 is the founding member of this family, and its activity appears to be dysregulated in heart disease an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi102022e
更新日期：2011-04-26 00:00:00
abstract：:A covalent complex between tRNA (m5U54)methyltransferase, 5-fluorouridine tRNA(Phe), and S-adenosyl-L-[methyl-3H]methionine was formed in vitro and purified. Previously, it was shown that in this complex the 6-position of fluorouridine-54 is covalently linked to a catalytic nucleophile and the 5-position is bound to t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00104a022
更新日期：1991-10-08 00:00:00
abstract：:The pigment composition of the isolated photosystem II reaction center complex in its most stable and pure form currently is a matter of considerable debate. In this contribution, we present a new method based on a combination of gel filtration chromatography and diode array detection to analyze the composition of pho...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960991m
更新日期：1996-10-01 00:00:00
abstract：:The effects of phosphorylation at Ser40 of rat tyrosine hydroxylase on the affinities of catechols have been determined with both the ferric and ferrous forms of the enzyme. Phosphorylation had no effect on the Ki value for the inhibition of the ferrous enzyme by either dopamine or DOPA when the initial rate of turnov...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980582l
更新日期：1998-06-23 00:00:00
