A reconstituted light-harvesting complex from the green sulfur bacterium Chlorobium tepidum containing CsmA and bacteriochlorophyll a.


:Green sulfur bacteria possess two light-harvesting antenna systems, the chlorosome and the Fenna-Matthews-Olson (FMO) protein. In addition to self-aggregated bacteriochlorophyll (BChl) c, chlorosomes of Chlorobium tepidum contain a small amount of BChl a (ratio 100:1). The chlorosomal BChl a is associated with CsmA, a 6.2 kDa protein that accounts for more than 50% of the protein content of chlorosomes. This CsmA-BChl a complex is located in the chlorosome baseplate with the hydrophilic C-terminal part of CsmA in contact with the FMO protein. CsmA was purified from Chl. tepidum. Isolated chlorosomes were lyophilized and extracted with chloroform/methanol (1:1, v/v). The extract was further purified using gel filtration and reverse-phase HPLC and the purity of the preparation confirmed by SDS-PAGE. Mass spectrometric analysis showed an m/z of 6154.8, in agreement with the calculated mass of the csmA gene product after C-terminal processing. CD spectroscopy of the isolated protein showed that the main structural motif was an alpha-helix. We have reconstituted the isolated CsmA protein with BChl a in micelles of n-octyl beta-d-glucopyranoside. The resulting preparation reproduced the spectral characteristics of the CsmA-BChl a complex present in the chlorosome baseplate.






Pedersen MØ,Pham L,Steensgaard DB,Miller M




Has Abstract


2008-02-05 00:00:00












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    pub_type: 杂志文章


    authors: Holowka DA,Cathou RE

    更新日期:1976-07-27 00:00:00

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    pub_type: 杂志文章


    authors: Itoh M,Kurokawa H,Usui M,Ohno M,Shimoda E,Hashimoto Y,Shudo K

    更新日期:1985-11-05 00:00:00

  • Structural Insights into the Inhibitory Mechanism of Insulin Secretagogues on the Pancreatic ATP-Sensitive Potassium Channel.

    abstract::Sulfonylureas and glinides are commonly used oral insulin secretagogues (ISs) that act on the pancreatic ATP-sensitive potassium (KATP) channel to promote insulin secretion in order to lower the blood glucose level. Physiologically, KATP channels are inhibited by intracellular ATP and activated by Mg-ADP. Therefore, t...


    pub_type: 杂志文章


    authors: Wu JX,Ding D,Wang M,Chen L

    更新日期:2020-01-14 00:00:00

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    pub_type: 杂志文章


    authors: Hutchinson JP,Eccleston JF

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    pub_type: 杂志文章


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  • Single-strand and double-strand deoxyribonucleic acid breaks produced by several bleomycin analogues.

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    pub_type: 杂志文章


    authors: Huang CH,Mirabelli CK,Jan Y,Crooke ST

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  • Evolvability of thermophilic proteins from archaea and bacteria.

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    pub_type: 杂志文章


    authors: Takano K,Aoi A,Koga Y,Kanaya S

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    pub_type: 杂志文章


    authors: Mårdh G,Vallee BL

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    pub_type: 杂志文章


    authors: Webb RJ,East JM,Sharma RP,Lee AG

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    pub_type: 杂志文章


    authors: Ma H,Peterson YK,Bernard ML,Lanier SM,Graber SG

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    pub_type: 杂志文章


    authors: Rodday SM,Webber AN,Bingham SE,Biggins J

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  • Mechanisms of specific and nonspecific binding of architectural proteins in prokaryotic gene regulation.

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    pub_type: 杂志文章


    authors: Benevides JM,Danahy J,Kawakami J,Thomas GJ Jr

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    pub_type: 杂志文章


    authors: Fabian M,Palmer G

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    pub_type: 杂志文章


    authors: Zhitkovich A,Song Y,Quievryn G,Voitkun V

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Ishihama A,Aiba H,Saitoh T,Takahashi S

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Dancheck B,Nairn AC,Peti W

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    pub_type: 杂志文章


    authors: Chan-Huot M,Sharif S,Tolstoy PM,Toney MD,Limbach HH

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    pub_type: 杂志文章


    authors: Browning KS,Leung DW,Clark JM Jr

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    pub_type: 杂志文章


    authors: Giri M,Maulik A,Singh M

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    pub_type: 杂志文章


    authors: Seymour KK,Lyons SD,Phillips L,Rieckmann KH,Christopherson RI

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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