Molecular cloning of bovine cardiac muscle heat-shock protein 70 kDa and its phosphorylation by cAMP-dependent protein kinase in vitro.


:The 70-kDa heat-shock protein (Hsp70) has been cloned and sequenced from bovine cardiac muscle. On the basis of sequence features, the gene corresponds to the cytoplasmic form of Hsp70. This cardiac Hsp70 cDNA clone has an open reading frame of 1926 bp coding for 641 amino acids and a predicted molecular mass of 70.25 kDa. Comparison of the amino acid sequence revealed an extensive sequence identity with other species of Hsp70. Escherichia coli expressed cardiac Hsp70 stimulated a 2-fold increase in calcineurin (CaN) activity. Notably, we observed that Hsp70 directly interacts with CaN using a pull-down assay. Furthermore, expressed cardiac-specific Hsp70 was phosphorylated in vitro by cAMP-dependent protein kinase. Phosphorylation resulted in the incorporation of 0.1 mol of phosphate per mol of Hsp70. The phosphorylated Hsp70 was unable to activate the phosphatase activity of CaN. This is the first demonstration that Hsp70 is phosphorylated by cAMP-dependent protein kinase and provides an on/off switch for the regulation of CaN signaling by Hsp70.






Lakshmikuttyamma A,Selvakumar P,Anderson DH,Datla RS,Sharma RK




Has Abstract


2004-10-26 00:00:00












  • Structural composition of canine secretory component and immunoglobulin A.

    abstract::Dog serum and colostral immunoglobulin A (IgA) and free secretory component from colostrum were isolated using affinity chromatography. Both serum and colostral IgA showed similar susceptibility to reduction with dithiothreitol, but only colostral IgA released the additional subunit, bound secretory component. This re...


    pub_type: 杂志文章


    authors: Thompson RE,Reynolds HY,Waxdal MJ

    更新日期:1975-07-01 00:00:00

  • 1H and 15N magnetic resonance assignments, secondary structure, and tertiary fold of Escherichia coli DnaJ(1-78).

    abstract::We report the 1H and 15N chemical shift assignments along with an NMR-derived preliminary structure for DnaJ(1-78), a highly conserved N-terminal domain of DnaJ, the Escherichia coli Hsp40 homolog. This 9 kDa domain is believed to cooperate with DnaK, the E. coli Hsp70 homolog, in regulating a variety of cellular func...


    pub_type: 杂志文章


    authors: Hill RB,Flanagan JM,Prestegard JH

    更新日期:1995-04-25 00:00:00

  • Comparative studies on the 7-iodo and 7-fluoro derivatives of N-acetoxy-N-2-acetylaminofluorene: binding sites on DNA and conformational change of modified deoxytrinucleotides.

    abstract::GMP and native DNA were reacted with 7-iodo and 7-fluoro derivatives of N-acetoxy-N-2-acetylaminofluorene. It was shown that the 7-halogeno derivatives react on C-8 of guanine. Furthermore the respective amount of arylamidation (covalent linkage on the C-8 of guanine) and arylation (covalent linkage on 2-NH2 groups of...


    pub_type: 杂志文章


    authors: Lefèvre JF,Fuchs RP,Daune MP

    更新日期:1978-06-27 00:00:00

  • Ion effects on the lac repressor--operator equilibrium.

    abstract::The effects of ions on the interaction of lac repressor protein and operator DNA have been studied by the membrane filter technique. The equilibrium association constant was determined as a function of monovalent and divalent cation concentrations, anions, and pH. The binding of repressor and operator is extremely sen...


    pub_type: 杂志文章


    authors: Barkley MD,Lewis PA,Sullivan GE

    更新日期:1981-06-23 00:00:00

  • Enthalpic and entropic effects of salt and polyol osmolytes on site-specific protein-DNA association: the integrase Tn916-DNA complex.

    abstract::The effect of low molecular-weight compounds on the equilibrium constant K(A) can be used to explore the energetics and molecular mechanism of protein-DNA interactions. Here we use the complex composed of the integrase Tn916 DNA-binding domain and its target DNA duplex to investigate the effects of salt and the nonion...


    pub_type: 杂志文章


    authors: Milev S,Bosshard HR,Jelesarov I

    更新日期:2005-01-11 00:00:00

  • An RNA polymerase mutant with reduced accuracy of chain elongation.

    abstract::A new Escherichia coli RNA polymerase mutant was isolated which exhibited reduced accuracy of chain elongation in vivo and in vitro. The novel isolation procedure consisted of simultaneous selection for rifampicin resistance and screening for increased leakiness of an early, strongly polar nonsense mutation of lacZ, o...


    pub_type: 杂志文章


    authors: Blank A,Gallant JA,Burgess RR,Loeb LA

    更新日期:1986-10-07 00:00:00

  • Bleaching kinetics of artificial visual pigments with modifications near the ring-polyene chain connection.

    abstract::Absorbance difference spectra were recorded at 20 degrees C from 30 ns to milliseconds after photolysis of lauryl maltoside suspensions of artificial visual pigments derived from 9-cis isomers of 5-ethylretinal, 8,16-methanoretinal (a 6-s-trans-bicyclic analogue), or 5-demethyl-8-methylretinal. In all three pigments, ...


    pub_type: 杂志文章


    authors: Szundi I,de Lera AR,Pazos Y,Alvarez R,Oliana M,Sheves M,Lewis JW,Kliger DS

    更新日期:2002-02-12 00:00:00

  • Human liver alanine aminopeptidase. Inhibition by amino acids.

    abstract::Human liver alanine aminopeptidase is inhibited by L-amino acids having hydrophobic side chains such as Phe, Tyr, Trp, Met, and Leu. Blocking of the amino group or the carboxyl group greatly reduces the inhibitory capacity of the amino acid. Kinetic studies demonstrate that inhibition of hydrolysis of the substrate L-...


    pub_type: 杂志文章


    authors: Garner CW,Behal FJ

    更新日期:1975-07-15 00:00:00

  • Evidence for functional and structural multiplicity of pregnenolone-16 alpha-carbonitrile-inducible cytochrome P-450 isozymes in rat liver microsomes.

    abstract::Administration of pregnenolone-16 alpha-carbonitrile (PCN) to adult female rats caused a 2-fold increase in total liver microsomal cytochrome P-450 along with 5-7-fold increases in four in vitro monooxygenase activities considered diagnostic for the major PCN-inducible cytochrome P-450 isozyme. However, upon administr...


    pub_type: 杂志文章


    authors: Graves PE,Kaminsky LS,Halpert J

    更新日期:1987-06-30 00:00:00

  • Measurement of the complexity and diversity of poly(adenylic acid) containing messenger RNA from rat liver.

    abstract::The complexity of rat liver poly (A)+ messenger RNA (mRNA) has been measured by analysis of the kinetics of hydridization with both complementary DNA (cDNA) and single copy DNA. The complementary DNA-poly(A)+ mRNA hybridization reaction demonstrates the existence of three abundance classes representing 18, 37, and 45%...


    pub_type: 杂志文章


    authors: Savage MJ,Sala-Trepat JM,Bonner J

    更新日期:1978-02-07 00:00:00

  • Phosphorescence studies of the interaction of myelin basic protein with phosphatidylserine vesicles.

    abstract::Phosphorescence from the lone tryptophan residue has been studied to monitor the interaction of myelin basic protein with phosphatidylserine vesicles. Spectral shifts in the phosphorescence of the protein in a glycerol-buffer (70:30 w/w) solvent at low temperature are consistent with fluorescence data obtained under a...


    pub_type: 杂志文章


    authors: Vadas EB,Melançon P,Braun PE,Galley WC

    更新日期:1981-05-26 00:00:00

  • Interaction of calcium and vanadate with fluorescein isothiocyanate labeled Ca2+-ATPase from sarcoplasmic reticulum: kinetics and equilibria.

    abstract::The interaction of Ca2+ and vanadate with fluorescein isothiocyanate (FITC) labeled sarcoplasmic reticulum (SR) Ca2+-ATPase has been studied by following the kinetics of changes in the reporter group fluorescence and equilibrium fluorescence levels. The vanadate species bound to the enzyme is clearly monomeric orthova...


    pub_type: 杂志文章


    authors: Markus S,Priel Z,Chipman DM

    更新日期:1989-01-24 00:00:00

  • Insights into the Mechanism of the Cyanobactin Heterocyclase Enzyme.

    abstract::Cyanobactin heterocyclases share the same catalytic domain (YcaO) as heterocyclases/cyclodehydratases from other ribosomal peptide (RiPPs) biosynthetic pathways. These enzymes process multiple residues (Cys/Thr/Ser) within the same substrate. The processing of cysteine residues proceeds with a known order. We show the...


    pub_type: 杂志文章


    authors: Ge Y,Czekster CM,Miller OK,Botting CH,Schwarz-Linek U,Naismith JH

    更新日期:2019-04-23 00:00:00

  • Cold denaturation of the molten globule states of apomyoglobin and a profile for protein folding.

    abstract::Protein folding is a process in which an extended polypeptide chain acquires compact packing through the formation of specific secondary and tertiary structures and hydrophobic interactions. Although much attention has been paid to secondary and tertiary structures, there is no definitive view about the relationship b...


    pub_type: 杂志文章


    authors: Nishii I,Kataoka M,Tokunaga F,Goto Y

    更新日期:1994-04-26 00:00:00

  • Physical dissection of the structural elements responsible for regulatory properties and intersubunit interactions of protein kinase CK2 beta-subunit.

    abstract::The noncatalytic beta-subunit of protein kinase CK2 has been shown to display various and in some respects antagonistic effects on the catalytic alpha-subunit [Boldyreff et al. (1993) Biochemistry 32, 12672-12677; Meggio et al. (1994) Biochemistry 33, 4336-4342]. We have now examined the ability of peptides encompassi...


    pub_type: 杂志文章


    authors: Marin O,Meggio F,Sarno S,Pinna LA

    更新日期:1997-06-10 00:00:00

  • Glutathione induces helical formation in the carboxy terminus of human glutathione transferase A1-1.

    abstract::The structure and dynamic properties of the C-terminal region of the human class alpha glutathione transferase A1-1 have been investigated with high-resolution NMR methods. On the basis of crystallographic and fluorescence measurements, this 13-residue segment of the enzyme is presumed to be disordered in the unligand...


    pub_type: 杂志文章


    authors: Zhan Y,Rule GS

    更新日期:2004-06-15 00:00:00

  • Insights into Cu(I) exchange in HAH1 using quantum mechanical and molecular simulations.

    abstract::The human antioxidant protein, HAH1, is an important participant in a Cu(I) transport chain, delivering one Cu(I) ion to the Wilson's (WND) or Menkes disease protein (MNK). Full geometry optimizations and second-derivative calculations were performed on several binding site models using the B3LYP functional to derive ...


    pub_type: 杂志文章


    authors: Op't Holt BT,Merz KM Jr

    更新日期:2007-07-31 00:00:00

  • Nitrogenase reactivity: cyanide as substrate and inhibitor.

    abstract::We have examined the reduction of cyanide by using the purified component proteins of nitrogenase (Av1 and Av2). The previously reported self-inhibition phenomenon was found to be an artifact. One of the two species present in cyanide solutions, CN-, was shown to be a potent reversible inhibitor (Ki = 27 microM) of to...


    pub_type: 杂志文章


    authors: Li J,Burgess BK,Corbin JL

    更新日期:1982-08-31 00:00:00

  • Using saturation-recovery EPR to measure distances in proteins: applications to photosystem II.

    abstract::The stable tyrosine radical YD. (tyrosine 160 in the D2 polypeptide) in photosystem II (PSII) exhibits nonexponential electron spin-lattice relaxation transients at low temperature. As previously reported, the tetranuclear Mn complex in PSII significantly enhances the spin-lattice relaxation of YD.. However, in Mn-dep...


    pub_type: 杂志文章


    authors: Hirsh DJ,Beck WF,Innes JB,Brudvig GW

    更新日期:1992-01-21 00:00:00

  • 2- and 8-azido photoaffinity probes. 2. Studies on the binding process of 2-5A synthetase by photosensitive ATP analogues.

    abstract::The photoaffinity probes [gamma-32P]2-azidoATP (2-N3ATP) and [alpha-32P]8-azido-ATP (8-N3ATP) were used to investigate the binding of ATP to highly purified 2-5A synthetase. 2-N3ATP and 8-N3ATP are substrates for 2-5A synthetase [Suhadolnik, R.J., Karikó, K., Sobol, R.W., Jr., Li, S.W., Reichenbach, N.L., & Haley, B.E...


    pub_type: 杂志文章


    authors: Suhadolnik RJ,Li SW,Sobol RW Jr,Haley BE

    更新日期:1988-11-29 00:00:00

  • Urea-induced unfolding of Na,K-ATPase as evaluated by electron paramagnetic resonance spectroscopy.

    abstract::Urea-induced unfolding of Na,K-ATPase from pig kidney and from shark salt gland was studied by electron paramagnetic resonance (EPR) spectroscopy of a nitroxyl derivative of maleimide covalently attached to sulfhydryl groups which are essential for activity. Urea-induced structural changes lead to the inhibition of Na...


    pub_type: 杂志文章


    authors: Babavali M,Esmann M,Fedosova NU,Marsh D

    更新日期:2009-09-29 00:00:00

  • Anisotropic dynamics of the JE-2147-HIV protease complex: drug resistance and thermodynamic binding mode examined in a 1.09 A structure.

    abstract::The structure of HIV protease (HIV Pr) bound to JE-2147 (also named AG1776 or KNI-764) is determined here to 1.09 A resolution. This highest-resolution structure for HIV Pr allows refinement of anisotropic displacement parameters (ADPs) for all atoms. Clustering based on the directional information in ADPs defines two...


    pub_type: 杂志文章


    authors: Reiling KK,Endres NF,Dauber DS,Craik CS,Stroud RM

    更新日期:2002-04-09 00:00:00

  • Importance of central alpha-helices of human apolipoprotein A-I in the maturation of high-density lipoproteins.

    abstract::We have studied the role of amphipathic alpha-helices in the ability of apoA-I to promote cholesterol efflux from human skin fibroblasts and activate lecithin:cholesterol acyltransferase (LCAT). Three apoA-I mutants were designed, each by deletion of a pair of predicted adjacent central alpha-helices [Delta(100-143), ...


    pub_type: 杂志文章


    authors: Frank PG,N'Guyen D,Franklin V,Neville T,Desforges M,Rassart E,Sparks DL,Marcel YL

    更新日期:1998-09-29 00:00:00

  • Distal site aspartate is essential in the catalase activity of catalase-peroxidases.

    abstract::Structural and biochemical characterization of aspartate 152 at the distal heme side of catalase-peroxidase (KatG) from Synechocystis PCC 6803 reveals an important functional role for this residue. In the wild-type protein, the side chain carboxyl group of Asp152 is 7.8 A apart from the heme iron and is hydrogen-bonde...


    pub_type: 杂志文章


    authors: Jakopitsch C,Auer M,Regelsberger G,Jantschko W,Furtmüller PG,Rüker F,Obinger C

    更新日期:2003-05-13 00:00:00

  • Stimulation of RecA-mediated D-loop formation by oligonucleotide-directed triple-helix formation: guided homologous recombination (GOREC).

    abstract::Oligonucleotide-directed triple helix formation provides an elegant rational basis for gene-specific DNA targeting and has been widely used to interfere with gene expression ("antigene" strategies) and as a molecular tool for biological studies. Various strategies have been developed to introduce sequence modification...


    pub_type: 杂志文章


    authors: Biet E,Maurisse R,Dutreix M,Sun Js

    更新日期:2001-02-13 00:00:00

  • Structural and Dynamic Insights into the Mechanism of Allosteric Signal Transmission in ERK2-Mediated MKP3 Activation.

    abstract::The mitogen-activated protein kinases (MAPKs) are key components of cellular signal transduction pathways, which are down-regulated by the MAPK phosphatases (MKPs). Catalytic activity of the MKPs is controlled both by their ability to recognize selective MAPKs and by allosteric activation upon binding to MAPK substrat...


    pub_type: 杂志文章


    authors: Lu C,Liu X,Zhang CS,Gong H,Wu JW,Wang ZX

    更新日期:2017-11-21 00:00:00

  • Uptake of cesium ions by human erythrocytes and perfused rat heart: a cesium-133 NMR study.

    abstract::Cesium-133 NMR studies have been carried out on suspended human erythrocytes and on perfused rat hearts in media containing CsCl. The resulting spectra exhibit two sharp resonances, arising from intra- and extracellular Cs+, separated in chemical shift by 1.0-1.4 ppm. Thus, intra- and extracellular resonances are easi...


    pub_type: 杂志文章


    authors: Davis DG,Murphy E,London RE

    更新日期:1988-05-17 00:00:00

  • Distinct phosphate backbone contacts revealed by some mutant peptides of zinc finger protein Spl: effect of protein-induced bending on DNA recognition.

    abstract::By using some mutant peptides of transcription factor Spl, phosphate backbone contacts with the DNA binding protein containing three zinc fingers have been investigated by alkylation interference, circular permutation, DNase I footprinting, and methylation protection methods. The ethylation interference analyses of Sp...


    pub_type: 杂志文章


    authors: Nagaoka M,Sugiura Y

    更新日期:1996-07-02 00:00:00

  • Local-access model for proton transfer in bacteriorhodopsin.

    abstract::The accessibility of the retinal Schiff base in bacteriorhodopsin was studied in the D85N/D96N mutant where the proton acceptor and donor are absent. Protonation and deprotonation of the Schiff base after pH jump without illumination and in the photocycle of the unprotonated Schiff base were measured in the visible an...


    pub_type: 杂志文章


    authors: Brown LS,Dioumaev AK,Needleman R,Lanyi JK

    更新日期:1998-03-17 00:00:00

  • Kinetics of polymerization of a fluoresceinated derivative of complement protein C9 by the membrane-bound complex of complement proteins C5b-8.

    abstract::The fluorescence self-quenching by energy transfer of FITC-C9, a fluoresceinated derivative of human complement protein C9 [Sims, P.J. (1984) Biochemistry (preceding paper in this issue)], has been used to monitor the kinetics of C9 polymerization induced by the membrane-associated complex of complement proteins C5b-8...


    pub_type: 杂志文章


    authors: Sims PJ,Wiedmer T

    更新日期:1984-07-03 00:00:00