Abstract：
:The effect of the polyene antibiotic amphotericin B on the electron paramagnetic resonance spectra of lipid probes intercalated in model membranes was examined. When the antibiotic was added to the aqueous phase, no spectral effects occurred. However, when the antibiotic was incorporated during membrane preparation, changes in spectral parameters suggested the appearance of a new phase. The spectral changes do not necessarily corroborate the pore models proposed previously for amphotericin B in membranes. With a spin probe that partitions between water and membrane, an interaction between the amphotericin B and probe is observed. This interaction does not occur in the membrane, but in the aqueous phase, between the probe and the aggregated antibiotic. Some of the equilibria involving the antibiotic appear to be achieved slowly.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Aracava Y,Smith IC,Schreier Sdoi
10.1021/bi00523a010subject
Has Abstractpub_date
1981-09-29 00:00:00pages
5702-7issue
20eissn
0006-2960issn
1520-4995journal_volume
20pub_type
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